Prevalence, antimicrobial resistance profiles and virulence-associated genes of thermophilic Campylobacter spp. isolated from ducks in a Chinese slaughterhouse

Human campylobacteriosis caused by thermophilic Campylobacter species is the most commonly reported foodborne zoonosis. Consumption of contaminated poultry meat is regarded as the main source of human infection. This study was undertaken to determine the antimicrobial susceptibility and the molecular epidemiology of 205 Campylobacter isolates derived from Greek flocks slaughtered in three different slaughterhouses over a 14-month period. A total of 98.5% of the isolates were resistant to at least one antimicrobial agent. In terms of multidrug resistance, 11.7% of isolates were resistant to three or more groups of antimicrobials. Extremely high resistance to fluoroquinolones (89%), very high resistance to tetracycline (69%), and low resistance to macrolides (7%) were detected. FlaA sequencing was performed for the subtyping of 64 C. jejuni and 58 C. coli isolates. No prevalence of a specific flaA type was observed, indicating the genetic diversity of the isolates, while some flaA types were found to share similar antimicrobial resistance patterns. Phylogenetic trees were constructed using the neighbor-joining method. Seven clusters of the C. jejuni phylogenetic tree and three clusters of the C. coli tree were considered significant with bootstrap values >75%. Some isolates clustered together were originated from the same or adjacent farms, indicating transmission via personnel or shared equipment. These results are important and help further the understanding of the molecular epidemiology and antimicrobial resistance of Campylobacter spp. derived from poultry in Greece.

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Microdilution testing reveals considerable and diverse antimicrobial resistance of Escherichia coli, thermophilic Campylobacter spp. and Salmonella spp. isolated from wild birds present in urban areas

European Journal of Wildlife Research ◽

10.1007/s10344-017-1125-2 ◽

2017 ◽

Vol 63 (4) ◽

Cited By ~ 6

Author(s):

Salome Troxler ◽

Claudia Hess ◽

Cornelia Konicek ◽

Zdeněk Knotek ◽

Pavel Barták ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Urban Areas ◽

Wild Birds ◽

Salmonella Spp ◽

Thermophilic Campylobacter ◽

Campylobacter Spp

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Prevalence, Antimicrobial Resistance, and Molecular Typing of Thermophilic Campylobacter Spp. in a Greek Poultry Slaughterhouse

Acta veterinaria ◽

10.2478/acve-2019-0027 ◽

2019 ◽

Vol 69 (3) ◽

pp. 325-339

Author(s):

Ioannis Sakaridis ◽

Theofilos Papadopoulos ◽

Evridiki Boukouvala ◽

Loukia Ekateriniadou ◽

Georgios Samouris ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Molecular Typing ◽

Environmental Samples ◽

Pfge Type ◽

Cross Contamination ◽

Thermophilic Campylobacter ◽

Antimicrobial Resistance Profile ◽

Relationship Of ◽

The Relationship ◽

Campylobacter Spp

Abstract Campylobacter species are one of the leading causes of foodborne disease. Poultry is a major reservoir and source of its transmission to humans. The aim of this study was to estimate the prevalence and antimicrobial resistance of Campylobacter spp. isolated from chicken carcasses, the environment, and processing equipment of a poultry slaughterhouse in Greece, to identify the dominant Campylobacter species and to determine if there are clonal relationships among the isolates. Fifty poultry samples and 25 environmental samples were examined using microbial cultures and PCR. Forty-nine of 50 poultry samples (98%) were found to be positive for Campylobacter spp. The environment of the slaughterhouse was also found to be significantly contaminated with Campylobacter spp. Thirty-seven isolates were found to be susceptible to all antimicrobials tested (56.1%) and 29 isolates showed resistance to at least two of the antimicrobials tested (43.9%). We observed 24 different PFGE-types among the 53 isolates with 14 of them isolated only once, while five PFGE-types were represented by two isolates. The remaining 29 isolates were represented by five PFGE-types each consisting of three to 12 isolates. Regarding the relationship of the PFGE types and corresponding resistance profiles, all strains of each PFGE-type shared the same antimicrobial resistance profile. This study reports evidence for Campylobacter spp. cross-contamination among broiler carcasses in a Greek slaughterhouse.

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Prevalence of thermophilic Campylobacter spp. in non-human primates of the Macaca mulatta species from a breeding center for use as biomodels

Revista Brasileira de Análises Clínicas ◽

10.21877/2448-3877.201800654 ◽

2018 ◽

Vol 50 (1) ◽

Author(s):

Paula Borba Cruz ◽

Fernanda Nunes Santos ◽

Wagner Thadeu Cardoso Esteves ◽

Jaime Antonio Abrantes ◽

Fabio Alves da Silva ◽

...

Keyword(s):

Macaca Mulatta ◽

Thermophilic Campylobacter ◽

Breeding Center ◽

Campylobacter Spp

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Impact of Ceftiofur Administration in Steers on the Prevalence and Antimicrobial Resistance of Campylobacter spp.

Microorganisms ◽

10.3390/microorganisms9020318 ◽

2021 ◽

Vol 9 (2) ◽

pp. 318

Author(s):

Sicun Fan ◽

Derek Foster ◽

William G. Miller ◽

Jason Osborne ◽

Sophia Kathariou

Keyword(s):

Antimicrobial Resistance ◽

Bacterial Resistance ◽

Active Drug ◽

Free Acid ◽

Structural Similarity ◽

Drug Concentrations ◽

Pre Treatment ◽

Ceftiofur Hydrochloride ◽

Campylobacter Spp

Bacterial resistance to ceftiofur raises health concerns due to ceftiofur’s extensive veterinary usage and structural similarity with the human antibiotic ceftriaxone. Ceftiofur crystalline-free acid (CCFA) and ceftiofur hydrochloride (CHCL) are ceftiofur types used therapeutically in cattle, but their potential impacts on Campylobacter prevalence and antimicrobial resistance remain unclear. In this study two groups of steers were each treated with CCFA or CHCL. In vivo active drug concentrations were measured and fecal samples were analyzed for Campylobacter for up to 42 days post-treatment. Following administration, the colonic concentration of ceftiofur initially increased then dropped to pre-treatment levels by day 8. The estimated prevalence of Campylobacter spp. was significantly (p = 0.0009) higher during the first week after CCFA treatment than after CHCL treatment (81.3% vs. 45.2%). Campylobacter jejuni predominated overall, with other Campylobacter spp. mainly identified in the first week after CCFA treatment. No treatment impacts were noted on ceftiofur minimum inhibitory concentration (MIC) for C. jejuni (10–20 μg/mL). More C. jejuni genotypes were detected in CCFA-treated than CHCL-treated steers. These findings suggest that ceftiofur did not significantly impact Campylobacter prevalence or ceftiofur MIC. However, CHCL may be preferable due to the lower likelihood of temporary increases in Campylobacter prevalence.

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Prevalence and Antimicrobial Susceptibility of Campylobacter spp. in Oklahoma Conventional and Organic Retail Poultry

The Open Microbiology Journal ◽

10.2174/1874285801408010130 ◽

2014 ◽

Vol 8 (1) ◽

pp. 130-137 ◽

Cited By ~ 24

Author(s):

Aneesa Noormohamed ◽

Mohamed K Fakhr

Keyword(s):

Multidrug Resistance ◽

Antimicrobial Resistance ◽

Foodborne Pathogens ◽

Antimicrobial Susceptibility ◽

Grocery Stores ◽

Campylobacter Spp

Campylobacteris one of the most important foodborne pathogens that cause bacterial gastroenteritis.This study was conducted to investigate the prevalence and antimicrobial resistance ofCampylobacterin conventional and organic retail poultry samples purchased from grocery stores in Tulsa, Oklahoma.One hundred and fifty six chilled retail chicken samples (85 conventional and 71 organic) and 65 chilled retail conventional turkey samples were collected in this study. The prevalence ofCampylobacterin the conventional chicken samples 32/85 (38%) was higher than in the organic ones 21/71 (30%). The prevalence ofCampylobacterin the conventional turkey samples was 11/65 (17%). Of the 53 positive chicken samples, 42 wereC. jejuni, 8 wereC. coliand three isolates were contaminated with both species. Of the 11 positive turkey samples, 8 containedC. jejuniand 3 harboredC. coliisolates. The antimicrobial susceptibility of one hundred and forty nine recoveredCampylobacterisolates (130 chickens and 19 turkeys) towards sixteen antimicrobials was determined. The majority of the recovered turkey isolates (13/19) showed resistance to more than 7 antimicrobials while most of the recovered chicken ones (82/130) were resistant to 5 to 7 antimicrobials. Multidrug resistance was not limited to isolates from conventional sources but was also available in isolates of an organic background and was generally lower inC. jejuniisolates when compared to theC. coliones.

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