Application of Exogenous Sodium Nitroprussid Alleviates Boron Toxicity in Wheat Seedlings: Investigation of Thiol Compounds, Macro/Micronutrient, and Polyamine Contents

SummaryTreatment of human and dog oxalated plasma with 0.2 to 1.0 × 10−1 M 2.3-dithiopropanol (BAL) or dithiothreitol (DTT) at 2–4° C for 30 min results in the reduction of the vitamin-K dependent clotting factors II, VII, IX and X to the respective-SH derivatives. The reaction is pH dependent. Under aerobic conditions the delayed one stage prothrombin time can be partly reversed. Under anaerobic conditions a gradual prolongation of the one stage prothrombin time occurs without reversal.In very diluted plasma treated with the dithiols, prothrombin can be converted into thrombin if serum as source of active factors VII and X is added. In contrast SH factors VII, IX and X are inactive in the specific tests. Reoxidation to active factors II, VII, IX and X takes place during adsorption and elution of the SH derivatives. The experiments have indicated that not only factor II but also factors VII, IX and X have active-S-S-centres.

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Lectin activity of winter wheat seedlings affected by eyespot causal agent under the action of Bacillus subtilis 537/Б1 bacterial isolates

Visnyk agrarnoi nauky ◽

10.31073/agrovisnyk201810-04 ◽

2018 ◽

Vol 96 (10) ◽

pp. 27-34

Author(s):

M. Musiienko ◽

L. Batsmanova ◽

Ju. Pys'menna ◽

T. Kondratiuk ◽

N. Taran ◽

...

Keyword(s):

Bacillus Subtilis ◽

Winter Wheat ◽

Causal Agent ◽

Bacterial Isolates ◽

Lectin Activity ◽

Wheat Seedlings

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Influence of the lipoic acid on the regenerative potential of thiol compounds in the retina in modeling age maculodystrophy in animals with allergic uveitis

Oftalmologicheskii Zhurnal ◽

10.31288/oftalmolzh201417480 ◽

2014 ◽

Vol 47 (1) ◽

pp. 74-80

Author(s):

V. Savko ◽

◽

Vashah Zijad Mahmud Ahmed ◽

Keyword(s):

Lipoic Acid ◽

Thiol Compounds

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Effect of 24-epibrassinolide on localization of ABA, AZP, and dehydrins in wheat plant roots during dehydration

Biomics ◽

10.31301/2221-6197.bmcs.2020-20 ◽

2020 ◽

Vol 12 (3) ◽

pp. 329-336

Author(s):

A.R. Lubyanova ◽

F.M. Shakirova ◽

M.V. Bezrukova

Keyword(s):

Triticum Aestivum ◽

Wheat Germ ◽

Wheat Plant ◽

Triticum Aestivum L ◽

Immunohistochemical Localization ◽

Plant Roots ◽

Wheat Roots ◽

Wheat Seedlings ◽

Apoplastic Barrier ◽

Protective Proteins

We studied the immunohistochemical localization of abscisic acid (ABA), wheat germ agglutinin (WGA) and dehydrins in the roots of wheat seedlings (Triticum aestivum L.) during 24-epibrassinolide-pretreatment (EB-pretreatment) and PEG-induced dehydration. It was found coimmunolocalization of ABA, WGA and dehydrins in the cells of central cylinder of basal part untreated and EB-pretreated roots of wheat seedlings under normal conditions and under osmotic stress. Such mutual localization ABA and protective proteins, WGA and dehydrins, indicates the possible effect of their distribution in the tissues of EB-pretreated wheat roots during dehydration on the apoplastic barrier functioning, which apparently contributes to decrease the water loss under dehydration. Perhaps, the significant localization of ABA and wheat lectin in the metaxylem region enhances EB-induced transport of ABA and WGA from roots to shoots under stress. It can be assumed that brassinosteroids can serve as intermediates in the realization of the protective effect of WGA and wheat dehydrins during water deficit.

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Cereals frost resistance correlates with an integral indicators of content of low-molecular-weight protective compounds and activity of antioxidant enzymes

Vìsnik Harkìvsʹkogo nacìonalʹnogo agrarnogo unìversitetu. Serìâ Bìologiâ ◽

10.35550/vbio2020.03.071 ◽

2020 ◽

Vol 2020 (3) ◽

pp. 71-86

Author(s):

O. I. Horielova ◽

◽

N. I. Ryabchun ◽

M. A. Shkliarevskyi ◽

A. M. Reznik ◽

...

Keyword(s):

Molecular Weight ◽

Superoxide Dismutase ◽

Antioxidant Enzymes ◽

Antioxidant System ◽

Frost Resistance ◽

Low Molecular Weight ◽

Guaiacol Peroxidase ◽

Wheat Seedlings ◽

Enzymatic Antioxidant ◽

Adult Plants

Along with specific adaptive reactions, universal defense reactions, in particular activation of antioxidant system, are of great importance for plant survival under cold conditions. We have studied a relationship among the content of low-molecular-weight protective compounds with antioxidant properties (proline, soluble carbohydrates, flavonoids), the activity of antioxidant enzymes (superoxide dismutase, catalase, and guaiacol peroxidase) in seedlings of winter wheat, rye and triticale, and frost resistance of etiolated seedlings and adult plants at tillering stage. It was found that there was a fairly close correlation between the frost resistance of seedlings and adult cereal plants (r = 0,78). It was shown that a pronounced relationship between individual indicators of antioxidant system functioning in unhardened seedlings and their frost resistance was not found. After 6-day hardening of seedlings at 2-4°C, there was a high correlation between the total indicator of the enzymatic antioxidant system (the sum of normalized indicators of superoxide dismutase, peroxidase, and catalase activity) and their frost resistance (r = 0,86), but the correlation coefficient of this index with frost resistance of plants in tillering phase was significantly lower (r = 0,47). At the same time, a high correlation was found between the content of low-molecular-weight protectors in hardened seedlings and frost resistance of tillering adult plants (r = 0.89). The closest correlation was observed between the integral normalized indicator, comprising the sum of normalized values of antioxidant enzymes activity and the content of low-molecular-weight protectors in hardened seedlings, and frost resistance of seedlings (r = 0,94) and plants in tillering phase (r = 0,89). A presence of specific features in the functioning of antioxidant system during cold adaptation of cereal seedlings was established. Rye is characterized by a high content of low-molecular-weight protective compounds; at the same time, increased activity of antioxidant enzymes - superoxide dismutase and catalase - was noted in wheat seedlings. In triticale, depending on the genotype, the values of both enzymatic antioxidant activity and the content of low-molecular-weight protectors varied.

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