Exogenous Zinc Induced NaCl Tolerance in Okra (Abelmoschus Esculentus) by Ameliorating Osmotic Stress and Oxidative Metabolism

Author(s):  
Muhammad Ali ◽  
Yasir Niaz ◽  
Ghulam Hassan Abbasi ◽  
Salman Ahmad ◽  
Zaffar malik ◽  
...  
1982 ◽  
Vol 60 (11) ◽  
pp. 2745-2752 ◽  
Author(s):  
M. E. Chamberlin ◽  
J. E. Phillips

Levels of individual and total (40–50 mM) amino acids remained relatively constant in locust hemolymph despite 50% changes in hemolymph volume during hydration and dehydration: proline, glycine, and glutamine constituted 70% of the total. Likewise, amino acid levels in tubular fluid did not change substantially during osmotic stress: proline constituted 80% of the total. Most hemolymph amino acids (P) appeared at lower concentrations in the tubular fluid (U); however, U/P ratios for glutamate and proline were 5.8 and 3.2, respectively. These results suggest that neutrally charged proline is actively transported by locust tubules, and this was confirmed by further experiments in vitro. Secreted proline is actively reabsorbed in the rectum where it probably serves as the principal substrate for oxidative metabolism in this tissue.


Author(s):  
Egbert W. Henry

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.


2019 ◽  
Vol 476 (22) ◽  
pp. 3521-3532
Author(s):  
Eric Soubeyrand ◽  
Megan Kelly ◽  
Shea A. Keene ◽  
Ann C. Bernert ◽  
Scott Latimer ◽  
...  

Plants have evolved the ability to derive the benzenoid moiety of the respiratory cofactor and antioxidant, ubiquinone (coenzyme Q), either from the β-oxidative metabolism of p-coumarate or from the peroxidative cleavage of kaempferol. Here, isotopic feeding assays, gene co-expression analysis and reverse genetics identified Arabidopsis 4-COUMARATE-COA LIGASE 8 (4-CL8; At5g38120) as a contributor to the β-oxidation of p-coumarate for ubiquinone biosynthesis. The enzyme is part of the same clade (V) of acyl-activating enzymes than At4g19010, a p-coumarate CoA ligase known to play a central role in the conversion of p-coumarate into 4-hydroxybenzoate. A 4-cl8 T-DNA knockout displayed a 20% decrease in ubiquinone content compared with wild-type plants, while 4-CL8 overexpression boosted ubiquinone content up to 150% of the control level. Similarly, the isotopic enrichment of ubiquinone's ring was decreased by 28% in the 4-cl8 knockout as compared with wild-type controls when Phe-[Ring-13C6] was fed to the plants. This metabolic blockage could be bypassed via the exogenous supply of 4-hydroxybenzoate, the product of p-coumarate β-oxidation. Arabidopsis 4-CL8 displays a canonical peroxisomal targeting sequence type 1, and confocal microscopy experiments using fused fluorescent reporters demonstrated that this enzyme is imported into peroxisomes. Time course feeding assays using Phe-[Ring-13C6] in a series of Arabidopsis single and double knockouts blocked in the β-oxidative metabolism of p-coumarate (4-cl8; at4g19010; at4g19010 × 4-cl8), flavonol biosynthesis (flavanone-3-hydroxylase), or both (at4g19010 × flavanone-3-hydroxylase) indicated that continuous high light treatments (500 µE m−2 s−1; 24 h) markedly stimulated the de novo biosynthesis of ubiquinone independently of kaempferol catabolism.


Planta Medica ◽  
2013 ◽  
Vol 79 (10) ◽  
Author(s):  
O Gordon ◽  
AC Ketron ◽  
N Osheroff ◽  
C Schneider

2017 ◽  
pp. 44-54
Author(s):  
Zenaida Gonzaga ◽  
Warren Obeda ◽  
Ana Linda Gorme ◽  
Jessie Rom ◽  
Oscar Abrantes ◽  
...  

Okra or Lady’s finger, botanically known as Abelmoschus esculentus (L.) Moench, is a tropical and sub-tropical indigenous vegetable crop commonly grown for its fibrous, slimy, and nutritious fruits and consumed by all classes of population. It has also several medicinal and economic values. Despite its many uses and potential value, its importance is under estimated, under-utilized, and considered a minor crop and little attention was paid to its improvement. The study was conducted to evaluate the effects of different planting densities and mulching materials on the growth and yield of okra grown in slightly sloping area in the marginal uplands in Sta. Rita, Samar, Philippines. A split-plot experiment was set up with planting density as main plot and the different mulching materials as the sub-plot which were: unmulched or bare soil, rice straw, rice hull, hagonoy and plastic mulch. Planting density did not significantly affect the growth and yield of okra. Regardless ofthe mulching materials used, mulched plants were taller and yielded higher compared to unmulched plants. Moreover, the use of plastic mulch resulted to the highest total fruit yield. The results indicate the potential of mulching in increasing yield and thus profitability of okra production under marginal upland conditions.


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