A preliminary comparison of the ammonium acetate‐edta soil phosphorus extraction method to the bray‐1 and olsen soil phosphorus extraction methods

Field experiments were conducted over 6 years at 33 sites throughout the main potato growing areas of South Australia to examine the effects of applied phosphorus (banded at planting), at rates up to 300 kg/ha, on the total yield and size distribution of tubers and to calibrate, in terms of total yield, 8 soil phosphorus extraction procedures (Colwell, Olsen, Bray 1, Bray 2, Mehlich no. 1, lactate, fluoride and total). Phosphorus application significantly (P< 0.05) increased total tuber yield at 16 sites. The mean relative yield for these responsive sites was 69.7% (range 37.4- 91.2%) compared with 97.5% (range 88.0-102.5%) for the non-responsive sites. Tuber size distributions were determined at 13 sites and, depending on site and cultivar, the yield of 80-450 g tubers for the highest yielding treatments represented from 64.2 to 93.7% of the total yield of tubers for those treatments. For each soil phosphorus extraction procedure the Mitscherlich and Smith-Dolby bent-hyperbola models and the Cate-Nelson separation were used to investigate the correlations between yield response and extractable and total phosphorus in the surface (0- 15 cm) soil samples and to calculate critical values. For loamy sand to sandy clay loam surface soils, the order of efficacy of soil tests based on the coefficients of determination (r2) calculated using the Mitscherlich and Smith-Dolby bent-hyperbola models was Bray 1 and Bray 2 > Olsen > lactate, Mehlich no. 1, fluoride and Colwell. The coefficients of determination ranged from 0.88 (Bray 1) to 0.64 (Colwell) for the Smith-Dolby bent-hyperbola model and from 0.86 (Bray 1) to 0.65 (fluoride) for the Mitscherlich model. Yield response was not correlated with total phosphorus concentration. Using the Smith-Dolby benthyperbola model the critical phosphorus values (s.e. in parentheses) were: 25.8(1.8), 40.9(2.6), l6.8(1.4), 13.9(1.0), 38.4(3.1), 24.2(2.9) and 35.1(3.0) mg/kg for the Bray 1, Bray 2, Olsen, lactate, fluoride, Mehlich no. 1 and Colwell methods, respectively. Yield deficits >20% were associated with phosphorus soil test values t 2 0 mg/kg (Bray 1 method) and P-sorption values >240 mg/kg. Rates of 48-73 kg P/ha banded at planting were required for 95% of maximum yield at the deficient sites. For acid coarse-grain sand surface soils, significant Cate-Nelson separations were obtained for the Colwell, Bray 1, Bray 2, Mehlich no. 1 and fluoride methods, the critical phosphorus values were 7.5, 7.0, 5.5, 6.5 and 8.0 mg/kg, respectively. The order of efficacy of the soil tests was Bray 2 (r2 = 0.66) >Bray 1, Colwell, Mehlich no. 1 and fluoride (all r2 = 0.55). Yield deficits >10% were associated with soil test values t 6 mg/kg (Bray 1 method). Rates of 27-59 kg P/ha banded at planting were required for 95% of maximum yield at the deficient sites. Data are presented which suggest that for similar soil types and extraction procedures critical values or critical concentration ranges may apply across a range of growing conditions, planting times and cultivars.

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Penentuan Metode Terbaik Uji Fosfor untuk Tanaman Tomat pada Tanah Inceptisols

Jurnal Hortikultura ◽

10.21082/jhort.v22n2.2012.p139-147 ◽

2013 ◽

Vol 22 (2) ◽

pp. 139

Author(s):

L Izhar ◽

A D Susila ◽

B S Puswoko ◽

A Sutandi ◽

I W Mangku

Keyword(s):

Extraction Method ◽

Soil Phosphorus ◽

Block Design ◽

Dry Weight ◽

Extraction Methods ◽

Soil P ◽

Soil P Test ◽

Coefficient Corrélation ◽

P Fertilizer ◽

Nutrient Extraction

ABSTRAK. Fosfor merupakan salah satu hara penting tanah dan aplikasi hara tersebut pada konsentrasi yang sesuai sangat berpengaruh terhadap pertumbuhan tomat. Penelitian tentang studi analisis fosfor tanah dan aplikasi pupuk fosfor pada budidaya tomat pada tanah Inceptisols dilakukan di Kebun Percobaan dan Rumah Kaca di Cikabayan, Institut Pertanian Bogor, dari bulan Maret sampai dengan November 2010. Tujuan penelitian ialah untuk mendapatkan metode ekstraksi fosfor tanah yang terbaik guna menentukan dosis pupuk fosfor pada budidaya tomat pada tanah Inceptisols. Penelitian menggunakan rancangan acak kelompok dengan perlakuan pemberian pupuk fosfor pada beberapa tingkat dosis yaitu 0X, ¼ X, ½ X, ¾ X, dan 1X, di mana nilai X ialah 368,5 kg/ha P2O5 dengan empat ulangan. Perlakuan pemupukan fosfor diterapkan pada 6 bulan sebelum penanaman tomat. Analisis korelasi dilakukan antara kandungan P tanah dan pertumbuhan tanaman yang ditanam di dalam rumah kaca menggunakan media inkubasi berasal dari tanah yang diberi perlakuan dan dianalisis. Uji fosfor tanah menggunakan lima metode ekstraksi, yaitu metode Bray I (NH4F 0,03 N + HCl 0,025 N, nisbah 1:7); Bray II (NH4F 0,03 N + HCl 0,10 N ); Mehlich I (HCl 0,05 N + H2SO4 0,025 N); Morgan Wolf (NaC2H2H3O2.3H2O; pH 4,8); dan Truogh [HCl 0,10 N + (NH4)2SO4; pH 3]. Hasil penelitian menunjukkan adanya perbedaan yang nyata antara pengaruh perlakuan pupuk P terhadap parameter tinggi tanaman, jumlah daun, dan diameter batang tomat. Bobot segar biomassa dan bobot kering tomat juga menunjukkan perbedaan pengaruh yang signifikan antarperlakuan. Nilai korelasi terbaik ditunjukkan oleh metode pengekstrak Mehlich I melalui parameter bobot kering dan bobot basah relatif tanaman. Dengan demikian, metode uji P tanah yang menggunakan Mehlich I dapat digunakan sebagai metode ekstraksi yang paling tepat untuk menganalisis unsur hara fosfor dengan koefisien korelasi 0,88, sehingga metode Mehlich I dapat diusulkan sebagai rekomendasi pemupukan P pada budidaya tomat pada tanah Inceptisols (nilai r = 0,89). <br /><br />ABSTRACT. Izhar, L, Susila, AD, Purwoko, BS, Sutandi, A, and Mangku, IW. 2012. Determination of the Best Method of Soil P Test for Tomato (Lycopersicon esculentum Mill. L) on Inceptisols Soil. Phosphorus is one of important soil elements and application of the element in suitable concentration give high effect on tomato growth. A study on phosphorus analysis and its application for recommendation of soil fertilization of tomato cultivation on Inceptisols soil was conducted at the field and Greenhouse of Cikabayan, Bogor Agricultural University, from March to November 2010. The objective of this research was to obtain the best extraction method of soil-P test for determining phosphorus nutrient required for tomato cultivation on Inceptisol soil. Rate of phosphorus of 0X, ¼ X, ½ X, ¾ X, and 1X, where X was 368.5 kg/ha P2O5 with four replications, was applied in the study. The treatments were applied 6 months before planting date. The research was arranged in randomized complete block design. Analysis of correlation between soil-P and plant growth based on data collected from the plants grown in the greenhouse using incubation media in treated-soil was analyzed. Soil-P test was carried out by using five extraction methods i.e. Bray I (HCl 0,025 N + NH4F 0.03), Bray II (NH4F 0.03 N + HCl 0.10 N), Mehlich I (HCl 0.05 N + H2SO4 0.025 N), Morgan Wolf (NaC2H2H3O2.3H2O; pH 4.8), and Truogh [HCl 0.10 N + (NH4)2SO4; pH 3]. The results showed that there were significant differences among the treatments of P fertilizer on the variables of plant height, leaf number, and stem diameter of tomato. Biomass fresh and dry weight of tomato also showed significantly different between the treatments applied. The highest correlation was shown on Mehlich I extraction reagent between plant dry and fresh weight. It means that, this P-nutrient extraction method was the most appropriate in determining phosphorus nutrient for tomatoes on Inceptisols soil with a coefficient correlation of 0.88. Mehlich I can also be used to develop a comprehensive phosphorus fertilizer recommendation for tomato cultivation on Inceptisols soil (r value = 0.89).<br /><br />

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Critical Level Determination of Phosphorus for Big Red Chili (Capsicum annuum L.) Growth on Soil in Java Island

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.10.3.190-199 ◽

2019 ◽

Vol 10 (3) ◽

pp. 190-199

Author(s):

Arief Hartono ◽

Desi Nadalia ◽

Siti Lathifah Husnul Khuluq

Keyword(s):

Extraction Method ◽

Critical Level ◽

Plant Biomass ◽

Growth Parameters ◽

Soil Analysis ◽

Dry Weight ◽

Extraction Methods ◽

P Fertilization ◽

Soil P ◽

Bray 1

One of the efforts that can be done so that the fertilization of phosphorus (P) accommodate the P needs of big red chili is by conducting soil analysis and determine the critical level of P in the soil related to the yield. The objective of this research was to determine the critical level of P for big red chili in Java Island. Soil samples were collected from 19 locations in Java Island. The soil P before planting of 19 locations was determined by three methods of P extraction namely Bray 1, Olsen, and Mehlich III. After soil P analyses, big red chili was planted in a pot containing 500 g of soil (oven-dry weight) from 19 locations where each location had 4 rates of P fertilization. The rates of P fertilization applied were 0, 55, 110, and 220 kg ha-1 P2O5. Urea and KCl were applied with the rate of 110 kg ha-1 and 180 kg ha-1 respectively as basal fertilizers. Plant biomass was harvested after the age of 4 weeks after planting. The effect of P fertilizer on growth parameters was evaluated using analysis of variance. The critical level of soil P was determined by the Cate and Nelson method. The results showed that P fertilization had a significant effect on plant height, fresh weight and dry weight of plants. Fertilization treatment of 220 kg ha-1 P2O5 resulted in the highest responses to plant growth parameters. The correlation test results showed that Bray 1, Olsen and Mehlich III extraction methods significantly and positively correlated with plant dry weight. The Olsen extraction method showed the highest correlation with plant dry weight (r=0.665). The critical levels of P in the soil for big red chili in Java determined by Bray 1, Olsen, and Mehlich III extraction methods, were 15 ppm, 40 ppm, and 50 ppm, respectively. Keywords: Bray 1, Cate and Nelson, extraction method, Mehlich, Olsen, soil analysis

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Sensitivity of soil phosphorus tests in predicting the potential risk of phosphorus loss from pasture soil

Agricultural and Food Science ◽

10.2137/145960608786118785 ◽

2008 ◽

Vol 17 (3) ◽

pp. 265 ◽

Cited By ~ 2

Author(s):

H. SOINNE ◽

K. SAARIJÄRVI ◽

M. KARPPINEN

Keyword(s):

Ammonium Acetate ◽

Soil Phosphorus ◽

Extraction Methods ◽

Low Ph ◽

Short Term ◽

Soil Extraction ◽

P Loss ◽

P Concentration ◽

Extractable P ◽

Total P

The objective of this study was to examine the effects of urine and dung additions on the phosphorus (P) chemistry of pasture land and to compare the sensitivity of two soil extraction methods in assessing the P-loading risk. In a field experiment, urine and dung were added to soil in amounts corresponding to single excrement portions and the soil samples, taken at certain intervals, were analysed for pHH2O, acid ammonium acetate extractable P (PAc) and water extractable total P (TPw), and molybdate reactive P (MRPw). Urine additions immediately increased soil pH and MRPw, but no such response was observed in PAc extraction due to the low pH (4.65) of the extractant enhancing the resorption of P. The PAc responded to the dunginduced increase in soil total P similarly as did Pw, which suggests that both tests can serve to detect areas of high P concentration. However, water extraction was a more sensitive method for estimating short-term changes in P solubility. In pasture soils, the risk of P loss increases as a result of the interaction of urination and high P concentration in the topsoil resulting from continuous dung excretion.;

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A Comparison of Different Soil Phosphorus Extraction Methods for Used to Determine Plant Available Soil Phosphorus of Erzurum Plain Agricultural Soils

International Journal of Innovative Research in Engineering & Management ◽

10.21276/ijirem.2017.4.3.1 ◽

2017 ◽

pp. 644-647

Author(s):

Nesrin YILDIZ ◽

Tülay DİZİKISA

Keyword(s):

Agricultural Soils ◽

Soil Phosphorus ◽

Extraction Methods ◽

Phosphorus Extraction ◽

Available Soil Phosphorus

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Relative Suitability of Phosphorus Extraction Procedures for F Tropical Ferralsol

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2020/v32i830315 ◽

2020 ◽

pp. 27-35

Author(s):

J. S. Tenywa ◽

E. Odama ◽

A. K. Amoding

Keyword(s):

Soil Phosphorus ◽

Extraction Methods ◽

P Uptake ◽

Strong Positive Correlation ◽

Predictive Capacity ◽

Soil P ◽

Application Rates ◽

Plant P Uptake ◽

Phosphorus Extraction ◽

P Application

Purpose: To evaluate the predictive capacity common procedures for soil P extraction and testing in laboratories in the region. Materials and Methods: A pot study with treatments viz. soil phosphorus extraction methods (Bray I, Bray II and Mehlich 3), and six P application rates (0, 20, 40, 60, 80 and 100 kg P ha-1). Maize (Zea mays L.) variety Longe IV was the test crop. A Ferralsol from northwestern Uganda (West Nile) was used in this study. Results and Conclusion: Mehlich 3 correlated most with plant P uptake by presenting the highest correlation coefficient with plant P content (r = 0.254) and a number of leaves per plant (r = 0.733). A strong positive correlation existed between Bray I and Mehlich 3 extractable P values (r = 0.975), suggesting lack of a marked difference between them; implying that either of the two procedures could be applied for soil P extraction in Ferralsols. However, Mehlich 3, being a multi-nutrient extractant, was recommended as the most suitable for P extraction for the Ferralsol used in this study.

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A comparison of 14 soil phosphorus extraction methods applied to 50 agricultural soils from Central Europe

Plant Soil and Environment ◽

10.17221/932/2014-pse ◽

2016 ◽

Vol 61 (No. 2) ◽

pp. 86-96 ◽

Cited By ~ 61

Author(s):

R. Wuenscher ◽

H. Unterfrauner ◽

R. Peticzka ◽

F. Zehetner

Keyword(s):

Central Europe ◽

Agricultural Soils ◽

Soil Phosphorus ◽

Extraction Methods ◽

Phosphorus Extraction

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Evaluation of DNA Extraction Methods Developed for Forensic and Ancient DNA Applications Using Bone Samples of Different Age

Genes ◽

10.3390/genes12020146 ◽

2021 ◽

Vol 12 (2) ◽

pp. 146

Author(s):

Catarina Xavier ◽

Mayra Eduardoff ◽

Barbara Bertoglio ◽

Christina Amory ◽

Cordula Berger ◽

...

Keyword(s):

Dna Extraction ◽

Ancient Dna ◽

Extraction Method ◽

Fragment Size ◽

Molecular Genetic ◽

Extraction Methods ◽

Degraded Dna ◽

Size Analysis ◽

Dna Fragments ◽

Dna Amount

The efficient extraction of DNA from challenging samples, such as bones, is critical for the success of downstream genotyping analysis in molecular genetic disciplines. Even though the ancient DNA community has developed several protocols targeting small DNA fragments that are typically present in decomposed or old specimens, only recently forensic geneticists have started to adopt those protocols. Here, we compare an ancient DNA extraction protocol (Dabney) with a bone extraction method (Loreille) typically used in forensics. Real-time quantitative PCR and forensically representative typing methods including fragment size analysis and sequencing were used to assess protocol performance. We used four bone samples of different age in replicates to study the effects of both extraction methods. Our results confirm Loreille’s overall increased gain of DNA when enough tissue is available and Dabney’s improved efficiency for retrieving shorter DNA fragments that is beneficial when highly degraded DNA is present. The results suggest that the choice of extraction method needs to be based on available sample, degradation state, and targeted genotyping method. We modified the Dabney protocol by pooling parallel lysates prior to purification to study gain and performance in single tube typing assays and found that up to six parallel lysates lead to an almost linear gain of extracted DNA. These data are promising for further forensic investigations as the adapted Dabney protocol combines increased sensitivity for degraded DNA with necessary total DNA amount for forensic applications.

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