Study on a novel oil-in-water-type microemulsion system of water/Triton X-100/Tween80/n-hexyl alcohol/n-octane

Attempts were made to maximize biomass production in the oxidation of fish oil by Candida lipolytica Y1094 and Geotrichum candidum Y552 in shake flasks. Maximum biomass was produced on 5% w/v oil in water containing 1.5% (NH4)2 SO4, 2.2% KH2PO4, 0.12% MgSO4, 0.03% fish stickwater solids, 10 ppm FeSO4 · 7H2O, 1.5 ppm MnSO4 · H2O, and adjusted to pH 7.5. For G. candidum 40 ppb biotin were added to the medium. When inoculum size was varied from 1 to 8% v/v, the most appropriate inoculum size was about 2%. Aeration rates were increased by decreasing the medium volume per 500-ml shake flask. Maximum yields were obtained with 25 ml of medium. Cell recoveries were complicated by formation of oil-media emulsions. Several methods of biomass recovery were studied. Warming the acidified cell suspension in the presence of Triton X-100 allowed removal of oil from the cell mass. Extraction of the dried cell mass with ethyl ether yielded highest recovery of cell material compared to other methods. Dry cells contained 40.1 to 49.3% crude protein. These studies suggest the feasibility of obtaining 800 g of single cell biomass with a crude protein yield of 320 g from 1 kg of fish oil using batch or continuous culture systems.

Download Full-text

Uji Stabilitas Sediaan Mikroemulsi Menggunakan Hidrolisat Pati (DE 35–40) Sebagai Stabilizer

Pharmaceutical Sciences and Research ◽

10.7454/psr.v3i1.3396 ◽

2006 ◽

Vol 3 (1) ◽

pp. 8-21

Author(s):

Mahdi Jufri ◽

◽

Effionora Anwar ◽

Putri Margaining Utami

Keyword(s):

Drug Delivery ◽

Dosage Form ◽

Tween 80 ◽

Microemulsion System ◽

Hydrophobic Drug ◽

Dextrose Equivalent ◽

Oil In Water ◽

External Phase ◽

The Stability ◽

Made In

Various solubilization techniques have been developed to enhance the bioavailability of hydrophobic drugs. One of the solubilization techniques is preparation of microemulsion. Microemulsion is a potential carrier in drug delivery system because it has many advantageous characteristics. In this research, hydrophobic drug was made in a dosage form of oil in water (O/W) microemulsion using ketoprofen as a model and investigated the influence of adding starch hydrolisates with dextrose equivalent (DE) 35-40 in variety concentrations (0,0%; 1,5%; 2,0%; 2,5%) to the stability of this microemulsion system. This microemulsion consisted of isopropyl miritate as oil phase, tween 80 and lechitin as surfactants, ethanol as cosurfactant, propylene glycol as cosolvent, starch hydrolisates DE 35Â–40 as stabilizer, and water as external phase. The evaluation was stability test both phisically and chemically. The result showed that the stability of microemulsion system increased significantly by adding starch hydrolisates DE 35-40 at 2,5%.

Download Full-text

Comparative Study on Surfactants Mixtures in Aqueous Solution at Atmospheric Pressure and 10 bar Reservoir Atmosphere

Periodica Polytechnica Chemical Engineering ◽

10.3311/ppch.12342 ◽

2018 ◽

Author(s):

Andrea Elekes ◽

Roland Nagy ◽

László Bartha ◽

Árpád Vágó

Keyword(s):

Crude Oil ◽

Petroleum Industry ◽

Test Methods ◽

Petroleum Exploration ◽

Process Conditions ◽

Water Type ◽

The Real ◽

Real Process ◽

Oil In Water ◽

Wide Range

This paper considers anionic and nonionic surfactants, as candidates for crude oil production by enhancement applications. In this study some colloidal properties of surfactants were tested by conventional and new test methods. The oil in water type emulsions have great importance in the petroleum industry. The stability of crude oil in water emulsions are investigated in a wide range of physical and chemical circumstances. Investigations at 10 bar are needed to get knowledges on the real conditions of the given petroleum exploration processes. A special glass cell was used for the tests of the mixtures various crude oil-water emulsions under hydrocarbon gas atmosphere and for the oil disclapement efficiency. Based on the experimental results it was found that emulsifying capacity was changed significantly by the real process conditions. The decrease of the density of hydrocarbon phase was contributed to the overall reduction in the efficiency of emulsifiers also occurred. Based on the experimented data it is supported to pay more attention to apply the real test conditions or approximate the real values.

Download Full-text

Influence of Cosurfactant on the Structure and Properties of ZrO2 Nano-Powders Prepared in Microemulsion System

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.368-372.729 ◽

2008 ◽

Vol 368-372 ◽

pp. 729-731

Author(s):

Chun Lei Geng ◽

Zhen Feng Zhu ◽

Wei Jie Yu

Keyword(s):

Specific Surface ◽

Xrd Analysis ◽

Microemulsion System ◽

Structure And Properties ◽

Distillation Process ◽

Mass Ratio ◽

Surface Areas ◽

Specific Surface Areas ◽

Average Size ◽

Triton X

ZrO2 nano-powders were prepared by a microemulsion process with the water-in-oil system of water/cyclohexane/Triton X-100/hexyl alcohol. The influence of cosurfactant content and the heterogeneous distillation process on the structure and properties of the particles was studied. XRD analysis showed that the cosurfactant content has little influence on the crystal phase of ZrO2. With the increasing mass ratio of cosurfactant to surfactant from 0.3 to 0.5 and 0.7, the average size of ZrO2 powders increases from 23.3 to 25.2 and 27.5 nm. The heterogeneous distillation process can effectively inhibit the formation of hard agglomeration during evaporation and increase the specific surface areas of ZrO2 powders.

Download Full-text

Triton X-100-stabilized "oil-in-water" emulsions as suitable media for alkalimetric determination of hydrophobic organic acids

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-002-1647-6 ◽

2003 ◽

Vol 375 (2) ◽

pp. 255-258 ◽

Cited By ~ 9

Author(s):

S. Kulichenko ◽

G. Shevchenko

Keyword(s):

Organic Acids ◽

Oil In Water ◽

Triton X

Download Full-text

Preparation and Characterization of Oil-in-Water Type Poly (D, L-Lactic Acid) Microspheres Containing Testosterone Enanthate

Drug Development and Industrial Pharmacy ◽

10.3109/03639049809097272 ◽

1998 ◽

Vol 24 (10) ◽

pp. 927-934 ◽

Cited By ~ 4

Author(s):

Satoru Tsubuku ◽

Shinya Sugawara ◽

Masaharu Miyajima ◽

Masaru Yoshida ◽

Masaharu Asano ◽

...

Keyword(s):

Lactic Acid ◽

Water Type ◽

Testosterone Enanthate ◽

Oil In Water

Download Full-text

Microemulsion-Based Solid Lipid Nanoparticles As Emulsion Stabilisers

10.32920/ryerson.14662881 ◽

2021 ◽

Author(s):

Elham H. Hazfi

Keyword(s):

Solid Lipid Nanoparticles ◽

Lipid Nanoparticles ◽

Continuous Phase ◽

Tween 20 ◽

Microemulsion System ◽

Solid Lipid ◽

Oil In Water ◽

Phase Crystal ◽

Surface Active ◽

Oil Water

The preparation and properties of water-in-oil (W/O) emulsions stabilised solely by adsorbed surface-active solid lipid nanoparticles (SLNs) at the oil-water interface were studied. Monostearin-based SLNs were prepared using food-grade micoremulsions as nanoscle 'reactors'. Hot oil-in-water (O/W) microemulsions (70°C) consisting of monostearin, Tween 20, ethanol and water were crash-cooled to 4°C to promote the liquid-solid transition of the monostearin and thus develop sub-micron solid lipid particles. SLNs obtained from the cooled microemulsions were partially stabilised with addition to lecithin (0.5% w/w) to the microemulsion system. With 2% (w/w) added monstearin, the W/O emulsion was stable for the 14 days of study. The microstructure of the emulsions revealed the presence of two stabilisation mechanisms, namely Pickering-type and continuous phase crystal network stabilisation, which both contributed to slowing dispersed droplet coalescence. Overall, this study demonstrated that surface-active SLNs developed using a microemulsion technique could effectively kinetically stabilise model W/O emulsions.

Download Full-text