Role of cyclooxygenase-2 in the development and treatment of oesophageal adenocarcinoma

Streptococcus suis is a common pathogen colonising the respiratory tract of pigs. It can cause meningitis, sepsis and pneumonia leading to economic losses in the pig industry worldwide. Cyclooxygenase-2 (COX-2) and its metabolites play an important regulatory role in different biological processes like inflammation modulation and immune activation. In this report we analysed the induction of COX-2 and the production of its metabolite prostaglandin E2 (PGE2) in a porcine precision-cut lung slice (PCLS) model. Using Western blot analysis, we found a time-dependent induction of COX-2 in the infected tissue resulting in increased PGE2 levels. Immunohistological analysis revealed a strong COX-2 expression in the proximity of the bronchioles between the ciliated epithelial cells and the adjacent alveolar tissue. The morphology, location and vimentin staining suggested that these cells are subepithelial bronchial fibroblasts. Furthermore, we showed that COX-2 expression as well as PGE2 production was detected following infection with two prevalent S. suis serotypes and that the pore-forming toxin suilysin played an important role in this process. Therefore, this study provides new insights in the response of porcine lung cells to S. suis infections and serves as a basis for further studies to define the role of COX-2 and its metabolites in the inflammatory response in porcine lung tissue during infections with S. suis.

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Radiation Induced Inflammatory Changes in the Mouse Bladder: The Role of Cyclooxygenase-2

The Journal of Urology ◽

10.1016/s0022-5347(05)00658-0 ◽

2006 ◽

Vol 175 (4) ◽

pp. 1529-1533 ◽

Cited By ~ 26

Author(s):

Jana Jaal ◽

Wolfgang Dörr

Keyword(s):

Cyclooxygenase 2 ◽

Radiation Induced ◽

Inflammatory Changes

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The role of cyclooxygenase-2 on endurance exercise training in female LDL-receptor knockout ovariectomized mice

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652013005000046 ◽

2013 ◽

Vol 85 (3) ◽

pp. 1157-1164 ◽

Cited By ~ 3

Author(s):

FLAVIA DE OLIVEIRA ◽

LAURA B.M. MAIFRINO ◽

GUSTAVO P.P. DE JESUS ◽

JULIANA G. CARVALHO ◽

CLAUDIA MARCHON ◽

...

Keyword(s):

Cardiovascular Risk ◽

Exercise Training ◽

Ldl Receptor ◽

Density Lipoprotein ◽

Cyclooxygenase 2 ◽

Sedentary Control ◽

Down Regulation ◽

Ovariectomized Mice ◽

Cox 2

Estrogen deprivation in postmenopausal women increases cardiovascular risk. Cardiovascular risk as a result of atherosclerosis is able to induce an inflammatory disease as far as cyclooxygenase-2 ( COX-2) expression. The purpose of the study was to investigate the role of COX-2 on exercise training in female mice low-density lipoprotein receptor knockout ( LDL-KO) with or without ovariectomy. A total of 15 female C57BL/6 mice and 15 female LDL-KO mice were distributed into 6 groups: sedentary control, sedentary control ovariectomized, trained control ovariectomized, LDL-KO sedentary, LDL-KO sedentary ovariectomized and LDL-KO trained ovariectomized. The ascending part of the aorta was stained with H&E and COX-2 expression was assessed by immunohistochemistry. Results revealed that ovariectomy as well as exercise training were not able to induce histopathological changes in mouse aorta for all groups investigated. LDL-KO mice demonstrated plaque containing cholesterol clefts, foamy histiocytes and mild inflammatory process for all groups indistinctly. Ovariectomy induced a strong immunoexpression in atherosclerosis lesion of LDL-KO mice. Nevertheless, a down-regulation of COX-2 expression was detected in LDL-KO trained ovariectomized when compared to LDL-KO sedentary. Our results are consistent with the notion that exercise training is able to modulate COX-2 expression in LDL-KO mice as a result of COX-2 down-regulation.

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Role of nuclear factor-κB in gastric ulcer healing in rats

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.2001.280.6.g1296 ◽

2001 ◽

Vol 280 (6) ◽

pp. G1296-G1304 ◽

Cited By ~ 15

Author(s):

Satoru Takahashi ◽

Takuya Fujita ◽

Akira Yamamoto

Keyword(s):

Gastric Ulcer ◽

Mrna Expression ◽

Ulcer Healing ◽

Nuclear Factor Κb ◽

Normal Mucosa ◽

Cyclooxygenase 2 ◽

Interleukin 1Β ◽

Nuclear Factor ◽

Gastric Ulcer Healing

We investigated the role of nuclear factor-κB (NF-κB) in gastric ulcer healing in rats. NF-κB was activated in ulcerated tissue but not in normal mucosa, and the level of the activation was decreased with ulcer healing. NF-κB activation was observed in fibroblasts, monocytes/macrophages, and neutrophils. Treatment of gastric fibroblasts, isolated from the ulcer base, with interleukin-1β activated NF-κB and the subsequently induced cyclooxygenase-2 and cytokine-induced neutrophil chemoattractant-1 (CINC-1) mRNA expression. Inhibition of activated NF-κB action resulted in suppression of both their mRNA expression and increases in PGE2 and CINC-1 levels induced by interleukin-1β. Persistent prevention of NF-κB activation caused an impairment of ulcer healing in rats. Gene expression of interleukin-1β, CINC-1, cyclooxygenase-2, and inducible nitric oxide synthase in ulcerated tissue had been inhibited before the delay in ulcer healing became manifest. The increased levels of cyclooxygenase-2 protein and PGE2 production were also reduced. These results demonstrate that NF-κB, activated in ulcerated tissue, might upregulate the expression of healing-promoting factors responsible for gastric ulcer healing in rats.

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Up-Regulation of Cyclooxygenase 2 and Matrix Metalloproteinases-2 and -9 in Cutaneous Squamous Cell Carcinoma: Active Role of Inflammation and Tissue Remodeling in Carcinogenesis

Annals of Dermatology ◽

10.5021/ad.2013.25.2.145 ◽

2013 ◽

Vol 25 (2) ◽

pp. 145 ◽

Cited By ~ 12

Author(s):

Jeong-Hoon Lee ◽

Mei Shan Piao ◽

Jee-Young Choi ◽

Sook Jung Yun ◽

Jee-Bum Lee ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Matrix Metalloproteinases ◽

Cell Carcinoma ◽

Squamous Cell ◽

Tissue Remodeling ◽

Active Role ◽

Cutaneous Squamous Cell Carcinoma ◽

Cyclooxygenase 2

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THE ROLE OF KI-67, P16, CD34, BCL-2, AND CYCLOOXYGENASE-2 IN THE PATHOGENESIS OF PROLIFERATIVE VERRUCOUS LEUKOPLAKIA

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology ◽

10.1016/j.oooo.2017.05.314 ◽

2017 ◽

Vol 124 (2) ◽

pp. e121

Author(s):

RAGHU RADHAKRISHNAN

Keyword(s):

Cyclooxygenase 2 ◽

Ki 67 ◽

Proliferative Verrucous Leukoplakia

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The Role of Propolis in Pulp Pain by Inhibiting Cyclooxygenase-2 Expression

Conservative Dentistry Journal ◽

10.20473/cdj.v11i1.2021.11-18 ◽

2021 ◽

Vol 11 (1) ◽

pp. 11

Author(s):

Ira Widjiastuti ◽

Widya Saraswati ◽

Annisa Rahma

Keyword(s):

Side Effects ◽

Pain Relief ◽

Inflammatory Pain ◽

Cyclooxygenase 2 ◽

Propolis Extract ◽

In Silico Studies ◽

Cox 2

Background: Inflammation of the pulp can lead to elicit pain. Pain in inflammation is induced by the cyclooxygenase-2 enzyme (COX-2) which induces prostaglandin E2 (PGE2) resulting in pain. Pain in the pulp can be relieved by eugenol. In its application, eugenol is toxic to pulp fibroblasts. Due to the side effect, it is worth considering other biocompatible materials with minimal side effects, such as propolis. Flavonoids and phenolic acids that contained in propolis can inhibit COX-2. Therefore, an analysis outlined in the literature review is needed to examine the results of research related to the role of propolis as pulp pain relief by inhibiting COX-2 expression. Purpose: To analyze the role of propolis in pulp pain by inhibiting COX-2 expression. Reviews: Propolis extract that extracted by ethanol, water, and hydroalcohol has pain relief properties in the pulp by inhibiting COX-2 by directly binding to the COX-2 receptors and by reducing the production of proinflammatory cytokines which are COX-2 inducers, proven through in vivo, in vitro, and in silico studies in various target cell organs. Conclusion: Propolis extract has high prospect as inflammatory pain inhibitor in the pulp by inhibit COX-2 expression.

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