Evaluating the effect of various bacterial consortia on antibacterial activity of marine Streptomyces sp. AC117

Streptomyces sp. strain AcE210 exhibited antibacterial activity toward Gram-positive microorganisms and turned out to be a rare producer of the specialized metabolite xanthocidin.

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ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM SOIL OF AD-DAWADMI, SAUDI ARABIA AND SCREENING THEIR ANTIBACTERIAL ACTIVITIES

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i10.15402 ◽

2017 ◽

Vol 9 (10) ◽

pp. 276 ◽

Cited By ~ 3

Author(s):

Gouse Basha Sheik ◽

Muazzam Sheriff Maqbul ◽

Gokul Shankar S. ◽

Ranjith M S

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Activities ◽

Soil Samples ◽

Streptomyces Sp ◽

Crude Extracts ◽

Test Organisms ◽

Inhibition Zones

Objective: To isolate and characterize novel actinomycetes and to evaluate their antibacterial activity against drug-resistant pathogenic bacteriaMethods: In the present study, 19 soil samples were collected from different localities of Ad-Dawadmi, Saudi Arabia. Actinomycetes were isolated from these samples using serial dilution and plating method on Actinomycetes isolation agar supplemented with nalidixic acid and actidione to inhibit bacteria and fungi. Crude extracts of potential actinomycetes were produced by submerged fermentation. The antimicrobial activity of crude extracts of actinomycetes was tested against different bacteria using the agar well diffusion method. Characterization of the isolates was done by morphological, physiological and biochemical methods.Results: A total of 9 (47%) isolates of actinomycetes were isolated from 19 different soil samples tested. Among them, 4 (44%) isolates confirmed as Streptomyces sp. showed potential antimicrobial activity against one or more test organisms. Crude extracts were made from these 4 actinomycetes isolates(DOM1, DOM3, DP3, DP4)and tested for their antibacterial activities against 4 different clinical bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus). Crude extract from DP3 isolate showed highest antibacterial activity against all the four test organisms (28 mm, 21 mm, 20 mm and 18 mm) respectively and DP4 showed lowest antibacterial activity against all the four test organisms (14 mm, 12 mm, 0 mm, 6 mm) respectively. The highest zone of inhibition was shown by DP3 against Staphylococcus aureus (28 mm) and Escherichia coli was resistant for DP4. Most of the Inhibition zones produced by crude extracts showed significant differences when compared with control, tested against test organisms (P<0.05). Inhibition zones produced by DP3 and DOM1 against Staphylococcus aureus were 28 mm and 23 mm, respectively which were strong active when compared with control Ciprofloxacin (18 mm).Conclusion: Further studies for purification of bioactive metabolites and molecular characterization analysis of isolated Streptomyces sp. are in progress which would be helpful in discovering novel compounds of commercial value.

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Antibacterial and antifungal activities of polyketide metabolite from marine Streptomyces sp. AP-123 and its cytotoxic effect

Chemosphere ◽

10.1016/j.chemosphere.2012.08.006 ◽

2013 ◽

Vol 90 (2) ◽

pp. 479-487 ◽

Cited By ~ 63

Author(s):

Mariadhas Valan Arasu ◽

Veeramuthu Duraipandiyan ◽

Savarimuthu Ignacimuthu

Keyword(s):

Cytotoxic Effect ◽

Streptomyces Sp ◽

Antifungal Activities ◽

Antibacterial And Antifungal Activities ◽

Marine Streptomyces ◽

Antibacterial And Antifungal

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Ala-geninthiocin, a new broad spectrum thiopeptide antibiotic, produced by a marine Streptomyces sp. ICN19

The Journal of Antibiotics ◽

10.1038/s41429-018-0115-2 ◽

2018 ◽

Vol 72 (2) ◽

pp. 99-105 ◽

Cited By ~ 12

Author(s):

Appadurai Muthamil Iniyan ◽

Enge Sudarman ◽

Joachim Wink ◽

Rajaretinam Rajesh Kannan ◽

Samuel Gnana Prakash Vincent

Keyword(s):

Broad Spectrum ◽

Streptomyces Sp ◽

Marine Streptomyces

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Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture

Revista de biología marina y oceanografía ◽

10.22370/rbmo.2018.53.2.1291 ◽

2018 ◽

Vol 53 (2) ◽

pp. 171 ◽

Cited By ~ 2

Author(s):

Mohammed Ishaque Nabila ◽

Krishnan Kannabiran

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Vibrio Harveyi ◽

Bacterial Pathogens ◽

Vibrio Anguillarum ◽

Antagonistic Activity ◽

Edwardsiella Tarda ◽

Streptomyces Sp ◽

Aeromonas Caviae ◽

Fish And Shellfish

A total of 72 morphologically different actinomycetes isolates were isolated from samples collected at different regions of Vellore, Tamil Nadu, India and screened for its antibacterial activity against fish and shellfish pathogens. All actinomycetes isolates were screened for antibacterial activity by cross streak method against the selected fish and shellfish bacterial pathogens including Aeromonas caviae, Aeromonas hydroplila, Edwardsiella tarda, Vibrio anguillarum and Vibrio harveyi. Secondary screening of antagonistic isolates by well diffusion method leads to the identification of potential isolate. Culture conditions for the potential isolate were optimized for maximal growth and yield of the ethyl acetate (EA) crude extract. The potential isolate was characterized by molecular taxonomy and phylogeny and identified as Streptomyces species and named as Streptomyces sp. VITNK9. The 16S rDNA nucleotide sequence was searched through the GenBank database and showed 83% similarity to Streptomyces vinaceusdrappus. The EA extract prepared from Streptomyces sp. VITNK9 showed moderate antagonistic activity accessed by the formation of zone of growth inhibition against, Aeromonas caviae (15.33 mm), Aeromonas hydrophila (17.66 mm), Edwardsiella tarda (18.33 mm), Vibrio anguillarum (14.33 mm) and Vibrio harveyi (14.33 mm). The MIC value of EA extract was ranged between 0.03-0.125 mg mL-1. The GC-MS spectrum of the ethyl acetate extract revealed the presence of two major compounds, pyrrolo [1,2-A] pyrazine-1,4-Dione (56.67%) and Hexahydro-3-(2-Methylpropyl) (27.91%), respectively. The results of the study suggest that Streptomyces sp. VITNK9 is a potential source for antagonistic secondary metabolites against fish and shellfish bacterial pathogens.

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