A study was conducted on 5 weeks old 64 (32 male and 32 female) Swiss albino mice to assess
the haemato-biochemical and immunological effects of acetamiprid. All the male and female mice
were randomly divided into eight different groups. The groups I (male) and II (female) served as
controls whereas remaining groups served as treatment groups and were administered acetamiprid
at the daily dose rate of 20, 10, 5 mg/kg body weight in males(Group III, V, VII) and females (Group
IV, VI,VIII),respectively for 28 days. After 28 days treatment, blood samples were collected for
hematological, biochemical as well as immunological analysis. There was significant decrease in
haematological parameters like Hb, TEC, TLC, neutrophils and lymphocytes count in high dose
groups and revealed potential adversity of acetamiprid at rates of 20 mg/kg/day on haematopoetic
system of mice. A dose dependent significant rise in mean values of AST and ALT was observed
in treatment groups, whereas there was significant decrease in total protein and albumin and
increase in BUN in high and mid dose treated groups, irrespective of sex of mice. Dinitroflurobenzene
(DNFB) test conducted to assess the cell mediated immunity revealed the toxic effect of acetamiprid
on cell mediated immunity of mice at dose level of 10 mg/kg/day. The mice of high dose group
revealed a significant decrease in HA titer and indicated the immunotoxic potential of acetamiprid
at dose level of 20 mg/kg/day.