Simple HPLC Method for the Determination of Thymoquinone in Black Seed Oil (Nigella Sativa Linn)

1995 ◽  
Vol 18 (5) ◽  
pp. 895-902 ◽  
Author(s):  
Hassan Y. Aboul-Enein ◽  
Laila I. Abou-Basha
2004 ◽  
Vol 15 (5) ◽  
pp. 320-324 ◽  
Author(s):  
Astrid Michelitsch ◽  
Anna Rittmannsberger ◽  
Antje Hüfner ◽  
Ulla Rückert ◽  
Werner Likussar

2020 ◽  
Vol 1469 ◽  
pp. 012022
Author(s):  
S E Priani ◽  
S S Maulidina ◽  
F Darusman ◽  
L Purwanti ◽  
D Mulyanti

2021 ◽  
Vol 7 (2) ◽  
pp. 132-137
Author(s):  
Afrina Mustari ◽  
Khaled Mahmud Sujan ◽  
Debasree Sarker Moni ◽  
Mahabub Alam ◽  
Mohammad Alam Miah ◽  
...  

Nigella sativa (Black seed) has been recognized as one of the most popular herbs in many parts of the world for centuries and used as folk medicine to cure different kinds of diseases. Vitamin E is well known for its antioxidative and anti-inflammatory properties, and has been studied in the prevention of cardiovascular disease (CVD). The present study was performed to determine the effect of black seed oil and Vit-E on growth performance and blood biochemistry in mice. A total of 40 mice weight between 25-27 gm were used for conducting the research. At first, the mice were randomly divided into 4 groups consisting 10 mice in each group. Group A served as the control group whereas Group B was administered black seed oil @ 0.5 ml/kg/day, Groups C was taken vitamin E @ 200 mg/kg/day and Group D was received both black seed oil @ 0.5 ml/kg/day + vitamin E @ 200 mg/kg/day respectively for 42 days. Result showed that, body weight of B, C and D group revealed a significant decrease than the control group (p<0.05). Hemoglobin concentration (Hb) and Total Erythrocyte Count (TEC) showed a significant change in B, C and D group than the control group (p<0.05). On the other hand, Total Leukocyte Count (TLC) and Packed Cell Volume (PCV) were insignificant. The results of biochemical test explored that serum cholesterol and triglyceride value decreased significantly whereas HDL increased significantly in black seed oil, Vit-E treated groups than the control group (p<0.05). But the LDL showed no change after treatment. It could be concluded that the black seed oil and Vit-E can be used in the therapeutic strategy of obesity, anemia and coronary diseases. Asian J. Med. Biol. Res. 2021, 7 (2), 132-137


Author(s):  
Sabah E Al-muhtaseb ◽  
Najah I Al-muhtaseb ◽  
Mahmoud Al-masri ◽  
Elham N Al-kaissi ◽  
Ibrahim S Al-adham ◽  
...  

Objective: Increasing use of medicinal plants in the treatment of infectious diseases are due to the development of multi-antibiotics resistant microorganisms, and had alerted our interest in the examination of some natural products. This study was carried out to investigate the antimicrobial activity of Jordanian propolis, black seed oil (Nigella sativa) extract, alone or in combination against clinically isolated microorganisms (bacteria and fungi).Methods: Jordanian propolis samples were collected. Aqueous and alcoholic extractions were done; black seed oil was extracted from Nigella sativa seeds. Seven clinical isolated microorganisms namely: Micrococcus luteus, Bacillus pumilus, Bordetella bronchisptica, Enterococcus fecalis, Bacillus subtilis, and Staphylococcus aureus, and one yeast strain namely Candida albicans were used. The antimicrobial activity was investigated by agar diffusion technique and microplate dilution to determine the MIC.Results: The results indicated that the alcoholic propolis extract showed higher antimicrobial activity than the aqueous propolis extract. The antimicrobial activity of black seed oil was significantly higher than that of the propolis. Mixing propolis with black seed oil showed synergism effects against some microorganisms as Enterococcus fecalis (24±1.1), Bordetella bronchisptica (20±0.9) and Candida albicans (40±2.3), and additive with others as Bacillus subtilis (28±1.8).Conclusion: Black seed oil and propolis might be used as a potential source of safe and effective natural antimicrobial in pharmaceutical and food industries.


2019 ◽  
Vol 47 (1) ◽  
pp. 59-65
Author(s):  
Sharmin Akter ◽  
Shanjida Sultana ◽  
Tangin Akter ◽  
Shefali Begum

The effectiveness of three different edible oils - black seed (Nigella sativa), sesame (Sesamum indicum) and soybean (Glycene max) was evaluated for the control of pulse beetle, Callosobruchus chinensis on green mung pulse, Vigna radiata. The oviposition deterrent and ovicidal activity of these three oils were compared between fresh mung pulses and pulses treated at different doses (1, 3 and 6 μl). The results indicated that the rate of oviposition and the mean number of emerged adults of C. chinensis on mung pulses treated with black seed, sesame and soybean oil were lower than their respective control pulses. The lowest number of eggs were recorded in soybean oil treated seeds (13.8 ± 1.07, 12.6 ± 1.36, 10.0 ± 1.82) followed by sesame (51.8 ± 4.63, 25.8 ± 8.52, 14.2 ± 4.50) and black seed oil (67.2 ± 9.71, 27.4 ± 5.52, 21.0 ± 5.54) at dose 1 μl, 3 μl and 6 μl, respectively in order. Soybean oil at the rate of 6μl/50 seeds was significantly effective (10.0 ± 1.82) to inhibit egg deposition. In case of ovicidal activity, minimum number of adults were emerged from black seed oil treated pulses (25.2 ± 2.73, 24.2 ± 0.58, 22.4 ± 1.17 at dose 1, 3 and 6 μl, respectively). Black seed oil at the rate of 6μl/ 50 seeds was the most effective (22.4 ± 1.17) than other edible oils. Bangladesh J. Zool. 47(1): 59-65, 2019


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