Steviol glycosides and bioactive compounds of a beverage with exotic fruits and Stevia rebaudiana Bert. as affected by thermal treatment

Abstract This study was conducted to find out the sweetener and bioactive compounds in the Stevia plant’s enzyme extract and its antioxidant and antibacterial effect. The enzyme extract was used in a ratio of 1:15 (w: v) with the use of sodium phosphate buffer solution (pH=4) in an equal mixing ratio with the enzyme. Extraction was carried out at 55°C with a time of 25 minutes to extract and determine steviol glycosides using HPLC. Stevioside and Rebaudioside-A (5.101 and 3.027 mg/g) were obtained, respectively. The study showed that Stevia contains high amounts of phenols and flavonoids (83.052 and 71,765) mg/ml, respectively. The enzyme extract gave an antioxidant activity of 71.367% compared to BHT which gave an activity of 77.267%. It gave the highest inhibitory activity against Bacillus subtilis, which was 14 mm, followed by Staphylococcus aureus and E. coli. The bioactive compounds were diagnosed by GC-MS and contained important bioactive compounds such as Hydroxydehydrostevic acid (steviol), 1,2-Benzenediol, 4-Ethylcatechol, 9-Octadecenamide, (Z)-, Isosteviol methyl ester, gamma-Sitosterol.

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Salt Stress Mitigation via the Foliar Application of Chitosan-Functionalized Selenium and Anatase Titanium Dioxide Nanoparticles in Stevia (Stevia rebaudiana Bertoni)

Molecules ◽

10.3390/molecules26134090 ◽

2021 ◽

Vol 26 (13) ◽

pp. 4090

Author(s):

Morteza Sheikhalipour ◽

Behrooz Esmaielpour ◽

Gholamreza Gohari ◽

Maryam Haghighi ◽

Hessam Jafari ◽

...

Keyword(s):

Titanium Dioxide ◽

Salt Stress ◽

Titanium Dioxide Nanoparticles ◽

Chitosan Nanoparticles ◽

Stevia Rebaudiana ◽

High Salt ◽

Steviol Glycosides ◽

Rebaudioside A ◽

Tio2 Nps ◽

Stevia Rebaudiana Bertoni

High salt levels are one of the significant and major limiting factors on crop yield and productivity. Out of the available attempts made against high salt levels, engineered nanoparticles (NPs) have been widely employed and considered as effective strategies in this regard. Of these NPs, titanium dioxide nanoparticles (TiO2 NPs) and selenium functionalized using chitosan nanoparticles (Cs–Se NPs) were applied for a quite number of plants, but their potential roles for alleviating the adverse effects of salinity on stevia remains unclear. Stevia (Stevia rebaudiana Bertoni) is one of the reputed medicinal plants due to their diterpenoid steviol glycosides (stevioside and rebaudioside A). For this reason, the current study was designed to investigate the potential of TiO2 NPs (0, 100 and 200 mg L−1) and Cs–Se NPs (0, 10 and 20 mg L−1) to alleviate salt stress (0, 50 and 100 mM NaCl) in stevia. The findings of the study revealed that salinity decreased the growth and photosynthetic traits but resulted in substantial cell damage through increasing H2O2 and MDA content, as well as electrolyte leakage (EL). However, the application of TiO2 NPs (100 mg L−1) and Cs–Se NPs (20 mg L−1) increased the growth, photosynthetic performance and activity of antioxidant enzymes, and decreased the contents of H2O2, MDA and EL under the saline conditions. In addition to the enhanced growth and physiological performance of the plant, the essential oil content was also increased with the treatments of TiO2 (100 mg L−1) and Cs–Se NPs (20 mg L−1). In addition, the tested NPs treatments increased the concentration of stevioside (in the non-saline condition and under salinity stress) and rebaudioside A (under the salinity conditions) in stevia plants. Overall, the current findings suggest that especially 100 mg L−1 TiO2 NPs and 20 mg L−1 Cs–Se could be considered as promising agents in combating high levels of salinity in the case of stevia.

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Comparative Assessment of the Basic Chemical Composition and Antioxidant Activity of Stevia rebaudiana Bertoni Dried Leaves, Grown in Poland, Paraguay and Brazil—Preliminary Results

Applied Sciences ◽

10.3390/app11083634 ◽

2021 ◽

Vol 11 (8) ◽

pp. 3634

Author(s):

Teresa Leszczyńska ◽

Barbara Piekło ◽

Aneta Kopeć ◽

Benno F. Zimmermann

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Vitamin C ◽

Dietary Fiber ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Stevia Rebaudiana ◽

Steviol Glycosides ◽

Total Polyphenols ◽

Stevia Rebaudiana Bertoni

This study compares the content of basic nutrients (proteins, fats, digestible carbohydrates, dietary fiber and ash), steviol glycosides, selected antioxidants (vitamin C, total polyphenols) and antioxidant activity in dried leaves of Stevia rebaudiana Bertoni cultivated in Poland, Paraguay and Brazil and available in the direct sale. The basic chemical composition was determined by standard AOAC (Association of Official Analytical Chemists) methods. Content of steviol glycosides was determined by the UHPLC-UV chromatographic method. Total polyphenols content was expressed as gallic acid equivalent (GAE) and catechins equivalent (CE). Antioxidant activity was measured as ABTS●+ free radical scavenging activity. Dried leaves of S. rebaudiana grown in Poland had significantly higher contents of dietary fiber, and lower protein and ash content, compared to those derived from Paraguay and Brazil. The former had, however, considerably higher contents of total steviol glycosides, stevioside and rebaudioside D, compared to the remaining two plants. In the Paraguay-derived dried leaves, the content of rebaudioside A, C, E and rubusoside was found to be significantly lower. Dried leaves of S. rebaudiana Bertoni, cultivated in Poland, contained substantially more vitamin C and a similar content of total polyphenols, compared to those from Brazil and Paraguay. The examined material from Brazil and Paraguay plantations showed similar antioxidant activity, while that obtained from Polish cultivation was characterized by a significantly lower value of this parameter.

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Attaining higher biomass and steviol glycosides yields of Stevia rebaudiana through adjustment of plant population and nitrogen rate

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.113426 ◽

2021 ◽

Vol 165 ◽

pp. 113426

Author(s):

Mitali Mahajan ◽

Anuradha ◽

Probir Kumar Pal

Keyword(s):

Stevia Rebaudiana ◽

Plant Population ◽

Steviol Glycosides ◽

Nitrogen Rate

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UHPLC Coupled with Charged Aerosol Detector for Rapid Separation of Steviol Glycosides in Commercial Sweeteners and Extract of Stevia Rebaudiana

SSRN Electronic Journal ◽

10.2139/ssrn.3875187 ◽

2021 ◽

Author(s):

Marcela Hollá ◽

Dalibor Šatínský ◽

František Švec ◽

Hana Sklenarova

Keyword(s):

Stevia Rebaudiana ◽

Steviol Glycosides ◽

Rapid Separation ◽

Charged Aerosol ◽

Charged Aerosol Detector

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Release Kinetic Studies of Stevia rebaudiana Extract Capsules from Sodium Alginate and Inulin by Ionotropic Gelation

Advances in Materials Science and Engineering ◽

10.1155/2018/6354924 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8

Author(s):

Juan Pablo Quintal Martínez ◽

Jorge Carlos Ruiz Ruiz ◽

Maira Rubí Segura Campos

Keyword(s):

Sodium Alginate ◽

Release Kinetics ◽

Stevia Rebaudiana ◽

Kinetic Studies ◽

Release Kinetic ◽

Steviol Glycosides ◽

Ionotropic Gelation ◽

Diffusion Controlled ◽

Release Analysis

This study was oriented towards encapsulation of S. rebaudiana extract and the study of its release kinetics. The desired encapsulation was achieved by the ionotropic gelation method using sodium alginate and inulin of polymeric constituents. Characterization of the capsules was performed by micrometric properties, encapsulation efficiency, in vitro extract release analysis, and biological activity of released extract. The in vitro release profiles from different capsules were applied on different kinetic models. The prepared capsules were found spherical in shape with diameters ranging from 2.07 to 2.63 mm, having the encapsulation efficiencies of 43.77% and 56.53% for phenolic compounds and steviol glycosides, respectively. The best-fit model with the highest correlation coefficient was observed in the Ritger–Peppas model, indicating diffusion controlled principle. The release exponent n value obtained from the Korsmeyer–Peppas model varied between 0.2273 and 1.1719, confirming that the mechanism of S. rebaudiana extract bioactive compounds release was diffusion controlled.

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Effect of the application of elicitors on the amount of steviol glycosides in Stevia rebaudiana Bertoni

2018 XIV International Engineering Congress (CONIIN) ◽

10.1109/coniin.2018.8489805 ◽

2018 ◽

Author(s):

Ma. Cristina Vazquez-Hernandez ◽

Aurora Alvarado-Mariana ◽

Humberto Aguirre-Becerra ◽

Genaro Martin Soto-Zarazua ◽

Ana Angelica Feregrino-Perez ◽

...

Keyword(s):

Stevia Rebaudiana ◽

Steviol Glycosides ◽

Stevia Rebaudiana Bertoni

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Comparison between Two Solid-Liquid Extraction Methods for the Recovery of Steviol Glycosides from Dried Stevia Leaves Applying a Numerical Approach

Processes ◽

10.3390/pr6080105 ◽

2018 ◽

Vol 6 (8) ◽

pp. 105 ◽

Cited By ~ 6

Author(s):

Monica Gallo ◽

Andrea Formato ◽

Gaetano Formato ◽

Daniele Naviglio

Keyword(s):

Stevia Rebaudiana ◽

Value Added ◽

Numerical Approach ◽

Extraction Process ◽

Extraction Methods ◽

Liquid Extraction ◽

Steviol Glycosides ◽

Stevia Rebaudiana Bertoni ◽

Solid Liquid Extraction ◽

Solid Liquid

Abstract: Stevia rebaudiana Bertoni is a perennial shrub belonging to the Asteraceae family. The leaves contain a mixture of steviol glycosides with extraordinary sweetening properties, among which the most important are stevioside and rebaudioside A. These components have a high sweetening power, which is about 300 times that of sucrose, and a negligible calorie content. However, their extraction and purification are not easy. In this paper, the extraction technique under cyclic pressure, known as rapid solid-liquid dynamic extraction (RSLDE), was compared using a Naviglio extractor (NE) with conventional maceration. The aim was to identify an efficient and economically viable method for obtaining high amounts of steviol glycosides in a short time. Furthermore, a numerical model was set up for the solid-liquid extraction process of value-added compounds from natural sources. Several parameters must be evaluated in relation to the characteristics of the parts of the plant subjected to extraction. Therefore, since diffusion and osmosis are highly dependent on temperature, it is necessary to control the temperature of the extraction system. On the other hand, the final aim of this work was to provide a scientific and quantitative basis for RSLDE. Therefore, the results obtained from stevia extracts using the corresponding mathematical model allowed hypothesizing the application of this model to the extraction processes of other vegetable matrices.

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