Constituents and antibacterial activity of leaf essential oil of Plectranthus scutellarioides

Author(s):  
Pakeeza Aziz ◽  
Nadeem Muhammad ◽  
Azeem Intisar ◽  
Muhammad Amin Abid ◽  
Muhammad Imran Din ◽  
...  
2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
José Cárdenas ◽  
Janne Rojas ◽  
Luís Rojas-Fermin ◽  
María Lucena ◽  
Alexis Buitrago

The essential oils from fresh aerial parts of Monticalia greenmaniana (Hieron) C. Jeffrey (Asteraceae) collected in March, were analyzed by GC/MS. Oil yields (w/v) of 0.1% (flowers), 0.07%, (stems) and 0.1% (leaves) were obtained by hydrodistillation. Thirteen, sixteen and eighteen components, respectively, were identified by comparison of their mass spectra with those in the Wiley GC-MS Library data base. The major components of the flower and stem oils were 1-nonane (38.8% flowers; 33.5% stems), α-pinene (29.0% flowers; 14.8% stems) and germacrene D (15.6% flowers; 18.6% stems). However, in the leaf oil, germacrene D was observed at 50.7%, followed by β-cedrene at 8.4 %. The leaf essential oil showed a broad spectrum of antibacterial activity against the important human pathogenic Gram-positive and Gram-negative bacteria Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 19433), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Klebsiella pneumoniae (ATCC 25955) with MIC values ranging from 75 to 6000 ppm.


2021 ◽  
Vol 20 (1) ◽  
pp. 23-29
Author(s):  
Opstaria Saptarini ◽  
Ismi Rahmawati

Kaffir lime leaves essential oil has activity to inhibit bacterial growth. The mechanism of the inhibition is thought to be located in thebacterial cell wall. This study aimed to determine the antibacterial work location of kaffir lime leaf essential oil against Staphylococcus aureus ATCC 25923. Kaffir lime leaves were distilled by steam distillation, essential oils obtained were identified by organoleptic quality, evaporation, solubility in alcohol, refractive index and specific gravity. The results were compared under with the literature. The results of essential oils were made with various concentrations of 100, 50, 25, 12.5, 6.25, 3.13, 1.56, 0.73, 0.39, and 0.20%. The results of the concentration series were tested for antibacterial activity with the macrodilution method followed by the determination for the location of bacterial growth inhibition using the Scanning Electron Microscope (SEM). The yield of kaffir lime leaf essential oil was 0.82%. The observation of organoleptic essential oil has a light yellow color, the distinctive odor of kaffir lime, a liquid form, and distinctive lime taste. Refractive index examination resulted 1.454; specific gravity 0.8317, and soluble in 70% ethanol with a ratio of 1:1. The Minimum BactericidalConcentration (MBC) obtained from the antibacterial activity test was 6.25%. Kaffir lime leaves essential oil mechanism occurred in the cell walls and cell membranes of Staphylococcus aureus. 


2010 ◽  
Vol 50 (S1) ◽  
pp. 70-73 ◽  
Author(s):  
A. Senthil Kumar ◽  
V. Venkatesalu ◽  
K. Kannathasan ◽  
M. Chandrasekaran

2019 ◽  
Vol 22 (6) ◽  
pp. 269-274
Author(s):  
Novena Risnalani Rintank Constani ◽  
Hartati Soetjipto ◽  
Sri Hartini

Peacock flower (Caesalpinia pulcherrima L.) leaves contain essential oils which can be used as an ingredient in cosmetics, perfume, aromatherapy, medicine, and supplements. The study was conducted to obtain essential oils from peacock flower leaves and determine the antibacterial activity against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa). Antibacterial activity test was carried out by the agar diffusion method, using paper discs. Measurements were made for the inhibition zone diameter (IZD) that appeared, while the essential oil component was analyzed using GC-MS. The results showed that the peacock flower leaves (C. pulcherrima) had a moderate to strong antibacterial effect at a concentration of 7.5%-20% against gram-positive bacteria (B. subtilis and S. aureus) and gram-negative bacteria (E. coli and P. aeruginosa). Gram-negative E. coli bacteria are relatively more sensitive to peacock flower leaf essential oil compared to other test bacteria. Peacock flower (C. pulcherrima) leaf essential oil is composed of 7 main components namely β-Cubebene 33.87%; Caryophyllene 23.00%; γ-Elemene 13.18%; α-Pinene 10.96%; Cadina-1(10),4-diene 10.20%; Copaene; 7.09%; β-Pinene 1.70%.


2009 ◽  
Vol 25 (7) ◽  
pp. 1297-1300 ◽  
Author(s):  
Annadurai Senthilkumar ◽  
Krishnan Kannathasan ◽  
Venugopalan Venkatesalu

2018 ◽  
Vol 66 (3) ◽  
pp. 207-214 ◽  
Author(s):  
H.V. Nguyen ◽  
J.-C. Meile ◽  
M. Lebrun ◽  
D. Caruso ◽  
S. Chu-Ky ◽  
...  

2009 ◽  
Vol 21 (4) ◽  
pp. 377-380 ◽  
Author(s):  
Henrik T. Simonsen ◽  
Casper Riedel ◽  
Lise B. Gade ◽  
Christina P. Jebjerg ◽  
Alfonso Guzman ◽  
...  

Author(s):  
Fenita Shoviantari

The essential oil of basil leaves (Oscimum basillicum L) has a mixture of chemical compositions, the most common of which are terpenes, aldehydes, alcohols, esters, phenols and ketones. Of these compounds that have antibacterial activity is linalool (51.2-74.73%) which is included in the terpenoid compound group. Objective: This study aims to determine whether there is an effect of the concentration of basil essential oil on the physical quality test of gel preparations and to determine whether there is a difference in the effect of the concentration of basil essential oil on antibacterial activity. The essential oil of basil leaves essential oil hand antiseptic gel was formulated with 4%, 6%, and 8% concentration of basil essential oil. Method: Antibacterial activity test was performed using the Disk Diffusion method. Results: The results of the research for the hand antiseptic gel of the essential oil of basil leaves (Oscimum basillicum L) met the parameters of the organoleptic test, homogeneity test, pH test, spreadability test and adhesion test. Basil leaf essential oil hand antiseptic gel with a concentration of 4% can inhibit bacterial growth with an average of 9mm, a concentration of 6% with an average of 10mm, and a concentration of 8% with an average of 12mm. By analyzing the data using One Way ANOVA, the Sig. 0,000 which means that there are differences in the three formulations. Conclusion: The three formulations are susceptible to strong inhibition.


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