scholarly journals Effect of hydrophilic polymers on the wettability, static and dynamic, of solid substrate covered by confluent monolayer of air-damaged SIRC cells

2015 ◽  
Vol 29 (2) ◽  
pp. 390-394 ◽  
Author(s):  
Petar Eftimov ◽  
Nadezhda Stefanova ◽  
Zdravko Lalchev ◽  
Georgi As. Georgiev
Keyword(s):  
Solid Substrate ◽  
Hydrophilic Polymers ◽  
Confluent Monolayer

Uultrastructure of Microtubules

1972 ◽  
Vol 30 ◽  
pp. 252-253
Author(s):  
Ariaki Nagayama
Keyword(s):  
Fine Structure ◽  
Cultured Cells ◽  
Present Report ◽  
Confluent Monolayer ◽  
Purification Method ◽  
Vinca Rosea ◽  
L Cells ◽  
Antimitotic Activity ◽  
Monolayer Cultures ◽  
Rapid Purification

Vinblastine(Vb) or vincristine, alkaloid derived from Vinca rosea is known for its antimitotic activity by regrouping of microtubules into paracrystalline form within the cells. A rapid purification method of vinblastine-induced microtubular paracrystals(PC) has provided us with a fresh and pure microtubular material demonstrating the presence of a labile ATPase associated with the PC. The present report is concerned with the fine structure of purified microtubules of mammalian cultured cells.Confluent monolayer cultures of L cells were incubated for 20hrs with 10-5 M Vb (donated from Shionogi Seiyaku & Co., Osaka, Japan).

Purification and Characterization of Natural Solid-Substrate Degrading and Alcohol Producing Hyperthermostable Alkaline Amylase from Bacillus cereus (sm-sr14)

2020 ◽  
Vol 21 (9) ◽  
pp. 872-881
Author(s):  
Sumit Sahoo ◽  
Sudipta Roy ◽  
Dipannita Santra ◽  
Sayantani Maiti ◽  
Sonali Roul ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Main Property ◽  
Solid Substrate ◽  
Industrial Applications ◽  
Starch Degradation ◽  
Significant Similarity ◽  
Alcohol Production ◽  
Cazy Database ◽  
Tim Barrel ◽  
Alkaline Amylase

Objective: Amylases enzymes hydrolyze starch molecules to produce diverse products including dextrins, and progressively smaller polymers. These include glucose units linked through α-1- 1, α-1-4, α-1-6, glycosidic bonds. Methods: This enzyme carrying an (α /β) 8 or TIM barrel structure is also produced containing the catalytic site residues. These groups of enzymes possess four conserved regions in their primary sequence. In the Carbohydrate-Degrading Enzyme (CAZy) database, α-amylases are classified into different Glycoside Hydrolase Families (GHF) based on their amino acid sequence. The present objective was to study one such enzyme based on its molecular characterization after purification in our laboratory. Its main property of solid-natural starch degradation was extensively investigated for its pharmaceutical/ industrial applications. Results: Amylase producing bacteria Bacillus cereus sm-sr14 (Accession no. KM251578.1) was purified to homogeneity on a Seralose 6B-150 gel-matrix and gave a single peak during HPLC. MALDITOF mass-spectrometry with bioinformatics studies revealed its significant similarity to α/β hydrolase family. The enzyme showed an efficient application; favourable Km, Vmax and Kcat during the catalysis of different natural solid starch materials. Analysis for hydrolytic product showed that this enzyme can be classified as the exo-amylase asit produced a significant amount of glucose. Conclusion: Besides the purified enzyme, the present organism Bacillus cereus sm-sr14 could degrade natural solid starch materials like potato and rice up to the application level in the pharmaceutical/ industrial field for alcohol production.

Modulation of amyloid β peptide aggregation by hydrophilic polymers

2019 ◽  
Vol 21 (37) ◽  
pp. 20999-21006
Author(s):  
Zhanna Evgrafova ◽  
Bruno Voigt ◽  
Andreas H. Roos ◽  
Gerd Hause ◽  
Dariush Hinderberger ◽  
...  
Keyword(s):  
Amyloid Β ◽  
Hydrophilic Polymers ◽  
Amyloid Β Peptide ◽  
Peptide Aggregation ◽  
Amyloidogenic Protein

Careful balance of hydrophilicity of precisely engineered polymers alters aggregation of the amyloidogenic protein Aβ1–40.

scholarly journals The microscopic distribution of hydrophilic polymers in interpenetrating polymer networks (IPNs) of medical grade silicone

Polymer ◽  
2021 ◽  
Vol 224 ◽  
pp. 123671
Author(s):  
Gregory N. Smith ◽  
Erik Brok ◽  
Martin Schmiele ◽  
Kell Mortensen ◽  
Wim G. Bouwman ◽  
...  
Keyword(s):  
Polymer Networks ◽  
Interpenetrating Polymer Networks ◽  
Hydrophilic Polymers ◽  
Microscopic Distribution ◽  
Medical Grade

scholarly journals Sarocladium and Lecanicillium Associated with Maize Seeds and Their Potential to Form Selected Secondary Metabolites

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 98
Author(s):  
Lidia Błaszczyk ◽  
Agnieszka Waśkiewicz ◽  
Karolina Gromadzka ◽  
Katarzyna Mikołajczak ◽  
Jerzy Chełkowski
Keyword(s):  
Large Subunit ◽  
Solid Substrate ◽  
Rrna Gene ◽  
Decenoic Acid ◽  
Bioactive Substances ◽  
Lecanicillium Lecanii ◽  
Maize Seeds ◽  
Internally Transcribed Spacer ◽  
First Time

The occurrence and diversity of Lecanicillium and Sarocladium in maize seeds and their role in this cereal are poorly understood. Therefore, the present study aimed to investigate Sarocladium and Lecanicillium communities found in endosphere of maize seeds collected from fields in Poland and their potential to form selected bioactive substances. The sequencing of the internally transcribed spacer regions 1 (ITS 1) and 2 (ITS2) and the large-subunit (LSU, 28S) of the rRNA gene cluster resulted in the identification of 17 Sarocladium zeae strains, three Sarocladium strictum and five Lecanicillium lecanii isolates. The assay on solid substrate showed that S. zeae and S. strictum can synthesize bassianolide, vertilecanin A, vertilecanin A methyl ester, 2-decenedioic acid and 10-hydroxy-8-decenoic acid. This is also the first study revealing the ability of these two species to produce beauvericin and enniatin B1, respectively. Moreover, for the first time in the present investigation, pyrrocidine A and/or B have been annotated as metabolites of S. strictum and L. lecanii. The production of toxic, insecticidal and antibacterial compounds in cultures of S. strictum, S. zeae and L. lecanii suggests the requirement to revise the approach to study the biological role of fungi inhabiting maize seeds.

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