Silk fibroin/hydroxyapatite scaffold: a highly compatible material for bone regeneration

After an osteosarcoma excision, recurrence and bone defects are significant challenges for clinicians. In this study, the curcumin (Cur) loaded chitosan (CS) nanoparticles (CCNP) encapsulated silk fibroin (SF)/hyaluronic acid esterified by methacrylate (HAMA) (CCNPs-SF/HAMA) hydrogel for the osteosarcoma therapy and bone regeneration was developed by photocuring and ethanol treatment. The micro or nanofibers networks were observed in the CCNPs-SF/HAMA hydrogel. The FTIR results demonstrated that alcohol vapor treatment caused an increase in β-sheets of SF, resulting in the high compression stress and Young’s modulus of CCNPs-SF/HAMA hydrogel. According to the water uptake analysis, SF caused a slight decrease in water uptake of CCNPs-SF/HAMA hydrogel while CCNPs could enhance the water uptake of it. The swelling kinetic results showed that both the CCNPs and the SF increased the swelling ratio of CCNPs-SF/HAMA hydrogel. The accumulative release profile of CCNPs-SF/HAMA hydrogel showed that the release of Cur from CCNPs-SF/HAMA hydrogel was accelerated when pH value was decreased from 7.4 to 5.5. Besides, compared with CCNPs, the CCNPs-SF/HAMA hydrogel had a more sustainable drug release, which was beneficial for the long-term treatment of osteosarcoma. In vitro assay results indicated that CCNPs-SF/HAMA hydrogel with equivalent Cur concentration of 150 μg/mL possessed both the effect of anti-cancer and promoting the proliferation of osteoblasts. These results suggest that CCNPs-SF/HAMA hydrogel with superior physical properties and the bifunctional osteosarcoma therapy and bone repair may be an excellent candidate for local cancer therapy and bone regeneration.

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Silk-based hybrid microfibrous mats as guided bone regeneration membranes

Journal of Materials Chemistry B ◽

10.1039/d0tb02687e ◽

2021 ◽

Author(s):

Mi Wu ◽

Zhengyi Han ◽

Wen Liu ◽

Jinrong Yao ◽

Bingjiao Zhao ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Cell Adhesion ◽

Bone Regeneration ◽

Silk Fibroin ◽

Guided Bone Regeneration ◽

Regenerated Silk Fibroin ◽

Marrow Mesenchymal Stem Cells ◽

Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

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Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach

Biomedical Materials ◽

10.1088/1748-605x/ac355a ◽

2021 ◽

Vol 17 (1) ◽

pp. 015003

Author(s):

Lya Piaia ◽

Simone S Silva ◽

Joana M Gomes ◽

Albina R Franco ◽

Emanuel M Fernandes ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Regeneration ◽

Bone Tissue ◽

Silk Fibroin ◽

Cellular Proliferation ◽

Mechanical Performance ◽

Tissue Growth ◽

Polymeric Scaffolds ◽

Bioactive Agents

Abstract Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth. By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process. Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions. The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering. Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60–170 μm, and a stiffness ranging from 1 to 2 MPa. Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture. Besides, cellular proliferation was higher on the scaffolds coated with silk. These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.

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Clinical study of guided bone regeneration of extracted socket with PLA/PGA membrane and silk fibroin membrane

The Journal of the Korean Academy of Periodontology ◽

10.5051/jkape.2009.39.2.129 ◽

2009 ◽

Vol 39 (2) ◽

pp. 129 ◽

Cited By ~ 3

Author(s):

Woo-Jin Hwang ◽

Seong-nyum Jeong ◽

Yun-Sang Kim ◽

Sung-Hee Pi ◽

Hyung-Keun You ◽

...

Keyword(s):

Clinical Study ◽

Bone Regeneration ◽

Silk Fibroin ◽

Guided Bone Regeneration

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Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration

Scientific Reports ◽

10.1038/s41598-018-25643-x ◽

2018 ◽

Vol 8 (1) ◽

Cited By ~ 14

Author(s):

Anuya Nisal ◽

Raeesa Sayyad ◽

Prachi Dhavale ◽

Bhakti Khude ◽

Rucha Deshpande ◽

...

Keyword(s):

Bone Regeneration ◽

Silk Fibroin ◽

Compression Modulus

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Bioinspired 3D porous human placental derived extracellular matrix/silk fibroin sponges for accelerated bone regeneration

Materials Science and Engineering C ◽

10.1016/j.msec.2020.110990 ◽

2020 ◽

Vol 113 ◽

pp. 110990

Author(s):

Arun Prabhu Rameshbabu ◽

Kamakshi Bankoti ◽

Sayanti Datta ◽

Elavarasan Subramani ◽

Anupam Apoorva ◽

...

Keyword(s):

Extracellular Matrix ◽

Bone Regeneration ◽

Silk Fibroin

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Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

Journal of Tissue Engineering ◽

10.1177/2041731420967791 ◽

2020 ◽

Vol 11 ◽

pp. 204173142096779

Author(s):

Ziquan Wu ◽

Zhulong Meng ◽

Qianjin Wu ◽

Delu Zeng ◽

Zhengdong Guo ◽

...

Keyword(s):

Bone Regeneration ◽

Bone Tissue ◽

Silk Fibroin ◽

Bone Repair ◽

Composite Scaffold ◽

Bone Tissue Regeneration ◽

Bone Mesenchymal Stem Cells ◽

Differentiation Potential ◽

Composite Effect ◽

Sd Rat

Artificial bioactive materials have received increasing attention worldwide in clinical orthopedics to repair bone defects that are caused by trauma, infections or tumors, especially dedicated to the multifunctional composite effect of materials. In this study, a weakly alkaline, biomimetic and osteogenic, three-dimensional composite scaffold (3DS) with hydroxyapatite (HAp) and nano magnesium oxide (MgO) embedded in fiber (F) of silkworm cocoon and silk fibroin (SF) is evaluated comprehensively for its bone repair potential in vivo and in vitro experiments, particularly focusing on the combined effect between HAp and MgO. Magnesium ions (Mg2+) has long been proven to promote bone tissue regeneration, and HAp is provided with osteoconductive properties. Interestingly, the weak alkaline microenvironment from MgO may also be crucial to promote Sprague-Dawley (SD) rat bone mesenchymal stem cells (BMSCs) proliferation, osteogenic differentiation and alkaline phosphatase (ALP) activities. This SF/F/HAp/nano MgO (SFFHM) 3DS with superior biocompatibility and biodegradability has better mechanical properties, BMSCs proliferation ability, osteogenic activity and differentiation potential compared with the scaffolds adding HAp or MgO alone or neither. Similarly, corresponding meaningful results are also demonstrated in a model of distal lateral femoral defect in SD rat. Therefore, we provide a promising 3D composite scaffold for promoting bone regeneration applications in bone tissue engineering.

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Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications

Journal of Nanomaterials ◽

10.1155/2019/8050413 ◽

2019 ◽

Vol 2019 ◽

pp. 1-16 ◽

Cited By ~ 4

Author(s):

Jihang Yao ◽

Yilong Wang ◽

Wendi Ma ◽

Wenying Dong ◽

Mei Zhang ◽

...

Keyword(s):

Drug Release ◽

Sustained Release ◽

Bone Regeneration ◽

Silk Fibroin ◽

Bone Morphogenetic Protein 2 ◽

Burst Release ◽

Nanofiber Membrane ◽

Differentiation Potential ◽

Dual Drug

Developing scaffold materials with excellent biocompatibility, mechanical properties, and controlled drug release properties is vital to tissue engineering. In this study, we fabricated silk fibroin (SF)/poly(lactide-co-glycolide) (PLGA) nanofiber scaffolds containing recombinant human bone morphogenetic protein 2 (rhBMP2) and dexamethasone (DXM) via coaxial electrospinning, which were used in in vitro bone formation with rat bone marrow mesenchymal stem cells (rBMSCs). An in vitro drug release study was adopted to evaluate the sustained release potential of the core-shell structured nanofibers. Furthermore, we detected the potential of the SF/PLGA nanofiber membrane in vitro. In vitro studies showed that rhBMP2 still remained active on the nanofiber membrane. In addition, the dual-drug-loaded nanofiber membrane showed an early burst release of DXM and late sustained release of rhBMP2. rhBMP2 and DXM exhibited strong osteogenic differentiation potential when they acted on rBMSCs. Therefore, the SF/PLGA nanofiber membrane loaded with rhBMP2 and DXM has great potential for the enhancement of bone regeneration.

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