Determination of free phenolic acids and antioxidant capacity of methanolic extracts obtained from leaves and flowers of camel thorn (Alhagi maurorum)

2011 ◽  
Vol 26 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Abdul Hafeez Laghari ◽  
Ayaz Ali Memon ◽  
Shahabuddin Memon ◽  
Aisha Nelofar ◽  
Khalid M. Khan ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Methanolic Extracts ◽  
Free Phenolic Acids

Determination of free phenolic acids and antioxidant activity of methanolic extracts obtained from fruits and leaves of Chenopodium album

2011 ◽  
Vol 126 (4) ◽  
pp. 1850-1855 ◽  
Author(s):  
Abdul Hafeez Laghari ◽  
Shahabuddin Memon ◽  
Aisha Nelofar ◽  
Khalid Mohammed Khan ◽  
Arfa Yasmin
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Chenopodium Album ◽  
Methanolic Extracts ◽  
Free Phenolic Acids

scholarly journals Physiologically Active Compounds in Four Species of Phellinus

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Katarzyna Sułkowska-Ziaja ◽  
Anna Maślanka ◽  
Agnieszka Szewczyk ◽  
Bożena Muszyńska
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Total Content ◽  
Dry Weight ◽  
Hplc Method ◽  
Indole Compounds ◽  
Methanolic Extracts ◽  
Free Phenolic Acids ◽  
Layer Chromatography ◽  
Physiologically Active Compounds

The content of two groups of compounds with biological activity (non-hallucinogenic indole compounds and free phenolic acids) were analyzed in extracts of fruiting bodies of four species of Phellinus: P. igniarius, P. pini, P. pomaceus and P. robustus. The presence of indole compounds in methanolic extracts was analyzed by high-performance liquid chromatography and thin-layer chromatography coupled with densitometric detection. Three metabolites (serotonin, tryptamine, and L-tryptophan) were identified. The contents of individual indole compounds ranged from 1.70 (tryptamine in P. robustus) to 8.32 mg x 100 g1 dry weight (L-tryptophan in P. robustus). Four free phenolic acids were detected in methanolic extracts by the HPLC method. The total content ranged from 9.9 mg x 100 g1 DW (P. igniarius) to 32.5 mg x 100 g1 DW (P. robustus).

Determination of Free Phenolic Acids in Wort and Beer by Coulometric Array Detection

2003 ◽  
Vol 51 (6) ◽  
pp. 1548-1554 ◽  
Author(s):  
Simona Floridi ◽  
Luigi Montanari ◽  
Ombretta Marconi ◽  
Paolo Fantozzi
Keyword(s):  
Phenolic Acids ◽  
Array Detection ◽  
Free Phenolic Acids

Determination of lignans, phenolic acids and antioxidant capacity in transformed hairy root culture of Linum usitatissimum

2017 ◽  
Vol 32 (15) ◽  
pp. 1867-1871 ◽  
Author(s):  
Ahmed M. M. Gabr ◽  
Hoda B. Mabrok ◽  
Emam A. Abdel-Rahim ◽  
Mohamed K. El-Bahr ◽  
Iryna Smetanska
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Hairy Root ◽  
Linum Usitatissimum ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Transformed Hairy Root

scholarly journals Anethum graveolens L. In Vitro Cultures – a Potential Source of Bioactive Metabolites, Phenolic Acids and Furanocoumarins

2015 ◽  
Vol 57 (2) ◽  
pp. 29-37 ◽  
Author(s):  
Agnieszka Szopa ◽  
Halina Ekiert
Keyword(s):  
Phenolic Acids ◽  
In Vitro Cultures ◽  
Bioactive Metabolites ◽  
Hydroxybenzoic Acid ◽  
Production Medium ◽  
Anethum Graveolens ◽  
Methanolic Extracts ◽  
Potential Source ◽  
Free Phenolic Acids

Abstract In vitro cultures of Anethum graveolens (dill) were maintained on the Linsmaier and Skoog (LS) medium – three variants, and the Murashige and Skoog (MS) medium – seven variants, which contained different amounts of plant growth regulators, cytokinin (BAP) and auxin (NAA) (from 0.1 mg l−1 to 3.0 mg l−1). Methanolic extracts from in vitro grown biomass were analyzed by HPLC for free phenolic acids and furanocoumarins. The total amounts of free phenolic acids on the LS medium variants were similar (35.23–38.65 mg 100 g−1 DW), but higher on the MS variants, ranging from about 66 mg 100 g−1 DW to 100 mg 100 g−1 DW. The main metabolites were: p-hydroxybenzoic acid (max. 24.41 mg 100 g−1 DW) on the LS−based media, and salicylic acid (max. 57.88 mg 100 g−1 DW) and p-hydroxybenzoic acid (max. 36.27 mg 100 g−1 DW) on the MS−based media. The total amounts of furanocoumarins were lower, as they did not exceed 8.5 mg 100 g−1 DW on the LS media and 25 mg 100 g−1 DW on the MS media. The main compounds in this group were bergapten (max. 15.01 mg 100 g−1 DW) and marmesin (max. 8.12 mg 100 g−1 DW). The MS variant containing 0.5 mg l−1 BAP and 2.0 mg l−1 NAA was proposed as the best production medium for both groups of metabolites. The maximum total amounts of free phenolic acids obtained in the in vitro grown biomass were slightly higher than their amounts in the fruits of the mother plant analyzed for comparison (99.66 mg 100 g−1 DW and 93.34 mg 100 g−1 DW, respectively); the maximum total amounts of furanocoumarins were approximately 1.8 times higher than in the fruits (24.26 mg 100 g−1 DW and 13.67 mg 100 g−1 DW, respectively).

scholarly journals Content of phenolic acids in rye caryopses determined using DAD-HPLC method

2013 ◽  
Vol 19 (No. 6) ◽  
pp. 201-205 ◽  
Author(s):  
R. Amarowicz ◽  
S. Weidner
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Hplc Method ◽  
Uv Spectra ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Uv Spectrum ◽  
Free Phenolic Acids

Phenolic compounds were extracted from rye caryopses with 80% (v/v) methanol. Phenolic acids were determined as free compounds and those liberated from soluble esters and glycosides. The analyses were performed using a Waters HPLC system equipped with a diode array detector (DAD). The following free phenolic acids were found: p-coumaric, ferulic and sinapic; the phenolic acids liberated from soluble esters were as follows: vanillic, caffeic, p-coumaric, ferulic and sinapic; and those liberated from soluble glycosides were the following: vanillic, p-coumaric, ferulic and sinapic. In rye caryopses, phenolic acids were chiefly in the form of soluble esters. A diode array detector was especially useful for the determination of vanillic acid: the UV spectrum of this compound showed a maximum at 260 nm whereas UV spectra of other phenolic acids were characterised by maxima at longer wavelengths.

Determination of Antioxidant Capacity, Phenolic Acids, and Fatty Acid Composition of Rapeseed Varieties

2010 ◽  
Vol 58 (13) ◽  
pp. 7502-7509 ◽  
Author(s):  
Aleksandra Szydłowska-Czerniak ◽  
Konrad Trokowski ◽  
György Karlovits ◽  
Edward Szłyk
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Antioxidant Capacity ◽  
Acid Composition ◽  
Phenolic Acids

scholarly journals Determination of free phenolic acids from leaves within different colored maize

2017 ◽  
Vol 82 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Jelena Mesarovic ◽  
Vesna Dragicevic ◽  
Snezana Mladenovic-Drinic ◽  
Danijela Ristic ◽  
Natalija Kravic
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Limit Of Detection ◽  
Principal Component ◽  
Dry Weight ◽  
Fermented Food ◽  
Array Detector ◽  
Maize Leaves ◽  
Free Phenolic Acids

Along with other plant parts, maize leaves are widely used for making fermented food for cattle, known as silage. Since there have been only a few reports on studies concerning the extraction and determination of phenolic acids from maize leaves, the main goal of this experiment was to evaluate free phenolic acids content in leaves of fifteen different maize inbred lines. Reverse-phase high performance liquid chromatography (RP-HPLC), equipped with photodiode array detector (DAD), was performed. Under the optimization of chromatographic conditions, referring to short time of samples preparation, small quantities of solvent and direct injection of extract into HPLC, phenolic acids (i.e. gallic, protocatechuic, caffeic, p-coumaric and ferulic acid) were successfully separated in less than 25 min, indicating that the method can be applied for routine analysis. The efficiency and validation of the method was evaluated by measuring the rate of parameters: linearity, limit of detection and quantification, accuracy and precision. Results obtained revealed that the most abundant free phenolic acid was p-coumaric acid (23.57 ?g g-1 dry weight), followed by ferulic and caffeic acids (21.27 and 20.78 ?g g-1 dry weight, respectively). Principal Component Analysis (PCA) revealed the existence of link.

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