Determination of free phenolic acids and antioxidant activity of methanolic extracts obtained from fruits and leaves of Chenopodium album

2011 ◽  
Vol 126 (4) ◽  
pp. 1850-1855 ◽  
Author(s):  
Abdul Hafeez Laghari ◽  
Shahabuddin Memon ◽  
Aisha Nelofar ◽  
Khalid Mohammed Khan ◽  
Arfa Yasmin
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Chenopodium Album ◽  
Methanolic Extracts ◽  
Free Phenolic Acids

Determination of free phenolic acids and antioxidant capacity of methanolic extracts obtained from leaves and flowers of camel thorn (Alhagi maurorum)

2011 ◽  
Vol 26 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Abdul Hafeez Laghari ◽  
Ayaz Ali Memon ◽  
Shahabuddin Memon ◽  
Aisha Nelofar ◽  
Khalid M. Khan ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Methanolic Extracts ◽  
Free Phenolic Acids

scholarly journals Physiologically Active Compounds in Four Species of Phellinus

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Katarzyna Sułkowska-Ziaja ◽  
Anna Maślanka ◽  
Agnieszka Szewczyk ◽  
Bożena Muszyńska
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Total Content ◽  
Dry Weight ◽  
Hplc Method ◽  
Indole Compounds ◽  
Methanolic Extracts ◽  
Free Phenolic Acids ◽  
Layer Chromatography ◽  
Physiologically Active Compounds

The content of two groups of compounds with biological activity (non-hallucinogenic indole compounds and free phenolic acids) were analyzed in extracts of fruiting bodies of four species of Phellinus: P. igniarius, P. pini, P. pomaceus and P. robustus. The presence of indole compounds in methanolic extracts was analyzed by high-performance liquid chromatography and thin-layer chromatography coupled with densitometric detection. Three metabolites (serotonin, tryptamine, and L-tryptophan) were identified. The contents of individual indole compounds ranged from 1.70 (tryptamine in P. robustus) to 8.32 mg x 100 g1 dry weight (L-tryptophan in P. robustus). Four free phenolic acids were detected in methanolic extracts by the HPLC method. The total content ranged from 9.9 mg x 100 g1 DW (P. igniarius) to 32.5 mg x 100 g1 DW (P. robustus).

Free Phenolic Acids from the Seaweed Halimeda monile with Antioxidant Effect Protecting against Liver Injury

2009 ◽  
Vol 64 (9-10) ◽  
pp. 657-663 ◽  
Author(s):  
Jorge Mancini-Filho ◽  
Alexis Vidal Novoa ◽  
Ana Elsa Batista González ◽  
Elma Regina S de Andrade-Wartha ◽  
Dalva Assunção Portari Mancini
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Chemical Agents ◽  
Liver Injuries ◽  
Dpph Method ◽  
Other Substances ◽  
Free Phenolic Acids

Phenolic compounds are found in seaweed species together with other substances presenting antioxidant activity. The objective of this work was to evaluate the antioxidant activity of the free phenolic acids (FPA) fraction from the seaweed Halimeda monile, and its activity to protect the expression of hepatic enzymes in rats, under experimental CCl4 injury. The antioxidant activity was measured by the DPPH method. The FPA fraction (80 mg/kg, p.o.) was administered during 20 consecutive days to rats. The peroxidation was performed by thiobarbituric acid reactive substances (TBARS). The SOD and CAT enzymatic expressions were measured by RT/PCR. The histology technique was used to evaluate liver injuries. The expression of both, CAT and SOD genes, was more preserved by FPA. Only partial injury could be observed by histology in the liver of rats receiving FPA as compared with the control group; and CCl4 administration induced 60% more peroxidation as compared with the rats receiving FPA. These data suggest that FPA could modulate the antioxidant enzymes and oxidative status in the liver through protection against adverse effects induced by chemical agents

Determination of Free Phenolic Acids in Wort and Beer by Coulometric Array Detection

2003 ◽  
Vol 51 (6) ◽  
pp. 1548-1554 ◽  
Author(s):  
Simona Floridi ◽  
Luigi Montanari ◽  
Ombretta Marconi ◽  
Paolo Fantozzi
Keyword(s):  
Phenolic Acids ◽  
Array Detection ◽  
Free Phenolic Acids

Determination of the nutritional and functional metabolites of Nanochloropsis gaditana produced in Algeria and evaluation of its antioxidant activity

2017 ◽  
Vol 7 (1) ◽  
pp. 13-20
Author(s):  
Asmaa Kherraf ◽  
Wafaa Tehami ◽  
Wahiba Boufeldja ◽  
Imène Yahla ◽  
Ghislaine Amira Dra ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Tannic Acid ◽  
Reducing Power ◽  
Matter Content ◽  
Bioactive Substances ◽  
Fresh Matter ◽  
Nannochloropsis Gaditana ◽  
Methanolic Extracts ◽  
Gallic Acid Equivalent

The aim of this work was to valorize the microalgae Nannochloropsis gaditana grown in Algeria for the purpose of producing human and animal food supplements as well as bioactive substances. The microalga had a fresh matter content of 9.33±1.15%. The amount of ash (6.4 ± 0.23%) was high and reflected mineral richness. Total sugars represented 7.13±0.23%. Proteins and lipids represented 13.38±0.2% and 7±1%, respectively. The ethanolic (EENg) and methanolic (MENg) extracts obtained from 10 g of lyophilisates of N. gaditana represented respectively 24±1.65% and 14.2±0.7%. The evalu-ation of the polyphenols in these extracts showed interesting rates repre-senting 41.53±0.41 mg gallic acid equivalent (GAE)/g of EENg dry extract and 38.72±0.33 mg GAE/g for the MENg. The flavonoid contents were 19.82±1.65 and 18.61±1.45 mg quercetin equivalent (QE)/g respectively for the ethanol-ic and methanolic extracts of this microalgae. The condensed and hydrolyza-ble tannins represented respectively 0.35±0.04 and 0.63±0.09 mg of tannic acid/g of dry EENg. They also represented respectively 0.50±0.08 and 0.18±0.02 mg of tannic acid/g of dry MENg. The antioxidant activity generat-ed by the extracts EENg and MENg against 2,2-diphenyl-1-picrylhydrazil (DPPH ) and by the reducing power of iron (FRAP) seems appreciable com-pared to that of ascorbic acid. These results are in favor of the development of the microalgae sector constituting a futuristic vision to allow the development of the sector with a view to sustainability.

scholarly journals Content of Phenolic Compounds and Antioxidant Activity of New Gluten-Free Pasta with the Addition of Chestnut Flour

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2623 ◽  
Author(s):  
Oniszczuk ◽  
Widelska ◽  
Wójtowicz ◽  
Oniszczuk ◽  
Wojtunik-Kulesza ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Unsaturated Fatty Acids ◽  
Antioxidant Properties ◽  
Polyphenolic Compounds ◽  
Field Bean ◽  
Gluten Free ◽  
Total Polyphenols ◽  
Free Phenolic Acids ◽  
Chestnut Flour

Chestnut fruit abounds in carbohydrates, proteins, unsaturated fatty acids, fiber, polyphenolic compounds, as well as vitamins and micronutrients, that are behind the health-promoting properties of this plant. The purpose of the discussed research was to obtain innovative gluten-free pasta from rice and field bean flour enriched with a various addition of chestnut flour. Regarding the studied pasta, the following were determined: the content of free phenolic acids, total polyphenols, and antioxidant properties. Chromatographic analysis (HPLC-ESI-MS/MS (high-performance liquid chromatography-electrospray ionization tandem mass spectrometry)) revealed a wide variety of phenolic acids. In a sample with 20% and higher content of chestnut flour, as many as 13 acids were detected. Isoferulic acid prevailed. The total content of free phenolic acids and total polyphenols increased along with the increasing chestnut content. Moreover, in most cases, the content of individual acids increased with the addition of chestnut flour. Besides, the antioxidant activity was positively correlated with the addition of chestnut fruit flour, the content of free phenolic acids, and total polyphenols. Our research has demonstrated that our innovative gluten-free pasta, with the addition of chestnut flour, has a potential to be a source of polyphenolic compounds, including free phenolic acids, that are valuable for human health.

scholarly journals Anethum graveolens L. In Vitro Cultures – a Potential Source of Bioactive Metabolites, Phenolic Acids and Furanocoumarins

2015 ◽  
Vol 57 (2) ◽  
pp. 29-37 ◽  
Author(s):  
Agnieszka Szopa ◽  
Halina Ekiert
Keyword(s):  
Phenolic Acids ◽  
In Vitro Cultures ◽  
Bioactive Metabolites ◽  
Hydroxybenzoic Acid ◽  
Production Medium ◽  
Anethum Graveolens ◽  
Methanolic Extracts ◽  
Potential Source ◽  
Free Phenolic Acids

Abstract In vitro cultures of Anethum graveolens (dill) were maintained on the Linsmaier and Skoog (LS) medium – three variants, and the Murashige and Skoog (MS) medium – seven variants, which contained different amounts of plant growth regulators, cytokinin (BAP) and auxin (NAA) (from 0.1 mg l−1 to 3.0 mg l−1). Methanolic extracts from in vitro grown biomass were analyzed by HPLC for free phenolic acids and furanocoumarins. The total amounts of free phenolic acids on the LS medium variants were similar (35.23–38.65 mg 100 g−1 DW), but higher on the MS variants, ranging from about 66 mg 100 g−1 DW to 100 mg 100 g−1 DW. The main metabolites were: p-hydroxybenzoic acid (max. 24.41 mg 100 g−1 DW) on the LS−based media, and salicylic acid (max. 57.88 mg 100 g−1 DW) and p-hydroxybenzoic acid (max. 36.27 mg 100 g−1 DW) on the MS−based media. The total amounts of furanocoumarins were lower, as they did not exceed 8.5 mg 100 g−1 DW on the LS media and 25 mg 100 g−1 DW on the MS media. The main compounds in this group were bergapten (max. 15.01 mg 100 g−1 DW) and marmesin (max. 8.12 mg 100 g−1 DW). The MS variant containing 0.5 mg l−1 BAP and 2.0 mg l−1 NAA was proposed as the best production medium for both groups of metabolites. The maximum total amounts of free phenolic acids obtained in the in vitro grown biomass were slightly higher than their amounts in the fruits of the mother plant analyzed for comparison (99.66 mg 100 g−1 DW and 93.34 mg 100 g−1 DW, respectively); the maximum total amounts of furanocoumarins were approximately 1.8 times higher than in the fruits (24.26 mg 100 g−1 DW and 13.67 mg 100 g−1 DW, respectively).

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