scholarly journals Transcriptome profiling revealed heat stress-responsive genes in Arabidopsis through integrated bioinformatics analysis

2021 ◽  
Vol 17 (1) ◽  
pp. 85-95
Author(s):  
Meili Guo ◽  
Xin Liu ◽  
Jiahui Wang ◽  
Yusu Jiang ◽  
Jinhuan Yu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Bioinformatics Analysis ◽  
Transcriptome Profiling ◽  
Stress Responsive Genes

scholarly journals Characterization of novel regulators for heat stress tolerance in tomato from Indian sub-continent

2019 ◽  
Author(s):  
Sonia Balyan ◽  
Sombir Rao ◽  
Sarita Jha ◽  
Chandni Bansal ◽  
Jaishri Rubina Das ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Functional Characterization ◽  
Transcriptome Profiling ◽  
Negative Regulator ◽  
Comparative Transcriptome ◽  
Regulatory Pathways ◽  
Cool Region ◽  
Promoter Swapping

AbstractThe footprint of tomato cultivation, a cool region crop that exhibits heat stress (HS) sensitivity, is increasing in the tropics/sub-tropics. Knowledge of novel regulatory hot-spots from varieties growing in the Indian sub-continent climatic zones could be vital for developing HS-resilient crops. Comparative transcriptome-wide signatures of a tolerant (CLN1621L) and sensitive (CA4) cultivar-pair short-listed from a pool of varieties exhibiting variable thermo-sensitivity using physiological, survival and yield-related traits revealed redundant to cultivar-specific HS-regulation with more up-regulated genes for CLN1621L than CA4. The anatgonisiticly-expressing genes include enzymes; have roles in plant defense and response to different abiotic stresses. Functional characterization of three antagonistic genes by overexpression and TRV-VIGS silencing established Solyc09g014280 (Acylsugar acyltransferase) and Solyc07g056570 (Notabilis), that are up-regulated in tolerant cultivar, as positive regulators of HS-tolerance and Solyc03g020030 (Pin-II proteinase inhibitor), that is down-regulated in CLN1621L, as negative regulator of thermotolerance. Transcriptional assessment of promoters of these genes by SNPs in stress-responsive cis-elements and promoter swapping experiments in opposite cultivar background showed inherent cultivar-specific orchestration of transcription factors in regulating transcription. Moreover, overexpression of three ethylene response transcription factors (ERF.C1/F4/F5) also improved HS-tolerance in tomato. This study identifies several novel HS-tolerance genes and provides proof of their utility in tomato-thermotolerance.HighlightNovel heat stress regulatory pathways uncovered by comparative transcriptome profiling between contrasting tomato cultivars from Indian sub-continent for improving thermotolerance. (20/30)

Transcriptome Profiling ofClematis apiifolia: Insights into Heat-Stress Responses

2017 ◽  
Vol 36 (11) ◽  
pp. 938-946 ◽  
Author(s):  
Lulu Gao ◽  
Yuzhu Ma ◽  
Peng Wang ◽  
Shu'an Wang ◽  
Rutong Yang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Responses ◽  
Transcriptome Profiling

Analysis of Heat-Stress Responsive Genes inAneurolepidium chinenseLeaves by Differential Display

2002 ◽  
Vol 5 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Weiming Shi ◽  
Akihiro Ueda ◽  
Keiko Ozaki ◽  
Mayumi Inada ◽  
Aya Takamatsu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Differential Display ◽  
Stress Responsive Genes

Transcriptome profiling of wheat genotypes under heat stress during grain-filling

2020 ◽  
Vol 91 ◽  
pp. 102895 ◽  
Author(s):  
Parimalan Rangan ◽  
Agnelo Furtado ◽  
Robert Henry
Keyword(s):  
Heat Stress ◽  
Transcriptome Profiling ◽  
Grain Filling ◽  
Wheat Genotypes

scholarly journals Integrated analysis of co-expression, conserved genes and gene families reveal core regulatory network of heat stress response in Cleistogenes songorica, a xerophyte perennial desert plant

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Qi Yan ◽  
Xifang Zong ◽  
Fan Wu ◽  
Jie Li ◽  
Tiantian Ma ◽  
...  
Keyword(s):  
Heat Stress ◽  
Comparative Analysis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Expression Analysis ◽  
Regulatory Network ◽  
Transcriptome Profiling ◽  
Gene Families ◽  
Differentially Expressed ◽  
Conserved Genes

Abstract Background As global warming continues, heat stress (HS) is becoming an increasingly significant factor limiting plant growth and reproduction, especially for cool-season grass species. The objective of this study was to determine the transcriptional regulatory network of Cleistogenes songorica under HS via transcriptome profiling, identify of gene families and comparative analysis across major Poaceae species. Results Physiological analysis revealed significantly decreased leaf relative water content (RWC) but increased proline (Pro) content in C. songorica under 24 h of HS. Transcriptome profiling indicated that 16,028 and 14,645 genes were differentially expressed in the shoots and roots of C. songorica under HS, respectively. Two subgenomes of C. songorica provide equal contribution under HS on the basis of the distribution and expression of differentially expressed genes (DEGs). Furthermore, 216 DEGs were identified as key evolutionarily conserved genes involved in the response to HS in C. songorica via comparative analysis with genes of four Poaceae species; these genes were involved in the ‘response to heat’ and ‘heat acclimation’. Notably, most of the conserved DEGs belonged to the heat-shock protein (HSP) superfamily. Similar results were also obtained from co-expression analysis. Interestingly, hub-genes of co-expression analysis were found to overlap with conserved genes, especially heat-shock protein (HSP). In C. songorica, 84 HSP and 32 heat-shock transcription factor (HSF) genes were identified in the allotetraploid C. songorica genome, and might have undergone purifying selection during evolutionary history based on syntenic and phylogenetic analysis. By analysing the expression patterns of the CsHSPs and CsHSFs, we found that the transcript abundance of 72.7% of the CsHSP genes and of 62.5% of the CsHSF genes changed under heat stress in both the shoots and roots. Finally, a core regulatory network of HS was constructed on the basis of the CsHSP, CsHSF and other responsive genes in C. songorica. Conclusions Regulatory network and key genes were comprehensively analysed and identified in C. songorica under HS. This study improves our knowledge of thermotolerance mechanisms in native grasses, and also provides candidate genes for potential applications in the genetic improvement of grasses.

scholarly journals Thermotolerant coral symbionts modulate heat stress‐responsive genes in their hosts

2020 ◽  
Vol 29 (15) ◽  
pp. 2940-2950
Author(s):  
Ross Cunning ◽  
Andrew C. Baker
Keyword(s):  
Heat Stress ◽  
Stress Responsive Genes

scholarly journals Melatonin Suppressed the Heat Stress-Induced Damage in Wheat Seedlings by Modulating the Antioxidant Machinery

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 809 ◽  
Author(s):  
Zeeshan Ali Buttar ◽  
Sheng Nan Wu ◽  
Marino B. Arnao ◽  
Chaojie Wang ◽  
Ikram Ullah ◽  
...  
Keyword(s):  
Heat Stress ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Oxygen Species ◽  
Defense System ◽  
Wheat Seedlings ◽  
Mda Content ◽  
Photosynthetic Machinery ◽  
Stress Responsive Genes ◽  
Antioxidant Machinery

Melatonin (N-acetyl-5-methoxytryptamine) is a pleiotropic signaling molecule that plays a crucial role in the regulation of various environmental stresses, including heat stress (HS). In this study, a 100 μM melatonin (MT) pretreatment followed by exposure to heat stress for different time periods was found to efficiently reduce oxidative stress by preventing the over-accumulation of hydrogen peroxide (H2O2), lowering the lipid peroxidation content (malondialdehyde (MDA) content), and increasing proline (Pro) biosynthesis. Moreover, the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), were increased substantially in MT-pretreated wheat seedlings. The presence of MT significantly improved the heat tolerance of wheat seedlings by modulating their antioxidant defense system, activating the ascorbate–glutathione (AsA–GSH) cycle comprising ascorbate peroxidase (APX), and increasing glutathione reductase (GR) activities. It also held the photosynthetic machinery stable by increasing the chlorophyll content. Enhancement in the endogenous MT contents was also observed in the MT+HS-treated plants. Furthermore, the expression of reactive oxygen species (ROS)-related genes TaSOD, TaPOD, and TaCAT, and anti-stress responsive genes, such as TaMYB80, TaWRKY26, and TaWRKY39, was also induced in MT-treated seedlings. Due to these notable changes, an improvement in stress resistance was observed in MT-treated seedlings compared with control. Taken together, our findings suggest that MT can play a key role in boosting the stress tolerance of plants by modulating the antioxidant defense system and regulating the transcription of stress-responsive genes.

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