Proliferative activity of various grades and types of breast carcinoma using AgNOR (Argyrophilic Nuclear Organizer Region) expression and its prognostic significance

Background: Breast cancers rarely occur in young women but are known to have more aggressive behaviors and poorer outcome. We here compare the significance of breast carcinoma in female below the age of 35 to the age over 35 whose specimens were submitted to Manipal teaching hospital, Pokhara. Materials and Methods: All cases of mastectomy with carcinoma from January 2000 to September 2011 were included in the study. Clinical and histopathological datas of all cases were reviewed and collated. Results: A total of 148 mastectomy specimens were received, among which, 23 cases (16%) were below 35 years; whereas 125 cases (84%) were above 35 years of age. In both groups, Stage II was the commonest stage but stage III was much more common in older group (33% versus 9%) and stage I was more common in younger age group (39% versus 27%). Bloom Richardson grading showed that in the older age group, grade 1 is the commonest grade (50%) while in the younger group; grade 3 is the commonest (39%). Patients were followed for a varying period of 6 months to 5 years. Two cases (2% of followed up cases) in older group and 3 cases (15% of followed up cases) in the younger group showed recurrence. Conclusion: Breast carcinoma in the patients younger than 35 years though presented at an early stage has higher grade tumor and poorer outcome. DOI: http://dx.doi.org/10.3126/jpn.v2i3.6021 JPN 2012; 2(3): 198-202

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Proliferative activity in breast carcinoma evaluated bv BrdU and PCNA

Pathology - Research and Practice ◽

10.1016/s0344-0338(11)80657-6 ◽

1995 ◽

Vol 191 (11) ◽

pp. 1122-1132 ◽

Cited By ~ 10

Author(s):

T. Moriki ◽

T. Takahashi ◽

F. Tanioka ◽

T. Yamane ◽

H. Hara

Keyword(s):

Breast Carcinoma ◽

Proliferative Activity

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The prognostic significance of proliferative activity in poor histology non-Hodgkin's lymphoma: a flow cytometry study using archival material

European Journal of Cancer and Clinical Oncology ◽

10.1016/0277-5379(87)90092-7 ◽

1987 ◽

Vol 23 (10) ◽

pp. 1497-1504 ◽

Cited By ~ 32

Author(s):

Graham A.R. Young ◽

David W. Hedley ◽

Cathy A. Rugg ◽

Harry J. Iland

Keyword(s):

Flow Cytometry ◽

Proliferative Activity ◽

Hodgkin’S Lymphoma ◽

Prognostic Significance ◽

Hodgkin's Lymphoma ◽

Archival Material ◽

Flow Cytometry Study ◽

Non Hodgkin’S Lymphoma ◽

Non Hodgkin's Lymphoma

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Expression of proliferation genes in formalin-fixed paraffin-embedded (FFPE) tissue from breast carcinomas. Feasibility and relevance for a routine histopathology laboratory

Journal of Clinical Pathology ◽

10.1136/jclinpath-2016-203786 ◽

2016 ◽

Vol 70 (1) ◽

pp. 25-32 ◽

Cited By ~ 1

Author(s):

Carla Thomas ◽

Cleo Robinson ◽

Ben Dessauvagie ◽

Benjamin Wood ◽

Greg Sterrett ◽

...

Keyword(s):

Gene Expression ◽

Breast Carcinoma ◽

Expression Profiling ◽

Proliferative Activity ◽

Breast Cancers ◽

Breast Carcinomas ◽

Ki 67 ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

AimBreast carcinoma proliferative activity, histological grade and commercial molecular tests are all important in prognostication and treatment. There is a particular need for improved, standardised techniques for subclassification of grade 2 breast cancers into low-risk and high-risk prognostic groups. In this study we investigated whether gene expression profiling of five proliferation genes was feasible using breast cancer tissue in a clinical setting and whether these profiles could enhance pathological assessment.MethodsExpression of five proliferation gene mRNAs; Ki-67, STK 15, CCNB1, CCND1 and MYBL2, was quantified in 27 breast carcinomas and compared with Ki-67 proliferation index (PI) and Nottingham mitotic score.ResultsExpression of Ki-67, STK15 and MYBL2 mRNA showed moderate Spearman's correlation with Ki-67 PI (p<0.01), but CCND1 and CCNB1 showed weak, non-significant correlation. Individual gene expression did not associate with mitotic score but combined mRNA expression correlated with both Ki-67 PI (p=0.018) and mitotic score (p=0.03; 0.007).ConclusionsThis study confirms mRNA analysis in breast carcinoma formalin-fixed, paraffin-embedded samples is feasible and suggests gene expression profiling, using a small set of five proliferation genes, has potential in aiding histological grading or assessment of proliferative activity of breast cancers. To fully evaluate the clinical applicability of this approach, a larger cohort study with long-term follow-up data is required.

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Proliferative Activity in Invasive Breast Carcinoma

Acta Cytologica ◽

10.1159/000328372 ◽

2001 ◽

Vol 45 (6) ◽

pp. 965-972 ◽

Cited By ~ 9

Author(s):

Mary L. Ostrowski ◽

Jana Pindur ◽

Rodolfo Laucirica ◽

Subhendu Chakraborty ◽

Ibrahim Ramzy

Keyword(s):

Breast Carcinoma ◽

Proliferative Activity ◽

Invasive Breast Carcinoma

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Oncogenes and Male Breast Carcinoma: c-erbB-2 and p53 Coexpression Predicts a Poor Survival

Journal of Clinical Oncology ◽

10.1200/jco.2000.18.16.2948 ◽

2000 ◽

Vol 18 (16) ◽

pp. 2948-2956 ◽

Cited By ~ 55

Author(s):

Achille Pich ◽

Elena Margaria ◽

Luigi Chiusa

Keyword(s):

Breast Carcinoma ◽

Prognostic Value ◽

Ductal Carcinoma ◽

Univariate Analysis ◽

Prognostic Significance ◽

Male Breast ◽

Male Breast Carcinoma ◽

P53 Immunoreactivity ◽

Mib 1

PURPOSE: To investigate the prognostic value of biomarkers in male breast carcinoma (MBC). PATIENTS AND METHODS: Fifty patients (mean age, 62.2 years) with invasive ductal carcinoma were retrospectively studied. All patients received surgery; 35 had adjuvant postoperative therapy. The median follow-up was 59 months (range, 1 to 230 months). c-myc, c-erbB-2, p53, and bcl-2 proteins were immunohistochemically detected on sections from formalin-fixed, paraffin-embedded tissues using 9E11, CB11, DO7, and bcl-2 124 monoclonal antibodies (mAbs). Estrogen, progesterone, and androgen receptors were detected using specific mAbs. Cell proliferation was assessed by MIB-1 mAb. RESULTS: In univariate analysis, c-myc, c-erbB-2, and p53 protein overexpression was significantly correlated with prognosis. The median survival was 107 months for c-myc–negative and 52 months for c-myc–positive patients (P = .01), 96 months for c-erbB-2–negative and 39 months for c-erbB-2–positive patients (P = .02), and 100 months for p53-negative and 33 months for p53-positive patients (P = .0008). Tumor histologic grade (P = .01), tumor size (P = .02), patient age at diagnosis (P = .03), and MIB-1 scores (P = .0004) also had prognostic value. In multivariate analysis, only c-erbB-2 and p53 immunoreactivity retained independent prognostic significance. All nine patients who did not express c-erbB-2 and p53 proteins were alive after 58 months, whereas none of the 14 patients expressing both proteins survived at 61 months follow-up (P = .0002). CONCLUSION: Overexpression of c-myc, c-erbB-2, and p53 proteins may be regarded as an additional prognostic factor in MBC. The combination of c-erbB-2 and p53 immunoreactivity can stratify patients into different risk groups.

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Prognostic Significance of Substance P/Neurokinin 1 Receptor and Its Association with Hormonal Receptors in Breast Carcinoma

BioMed Research International ◽

10.1155/2021/5577820 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Riffat Mehboob ◽

Syed Amir Gilani ◽

Amber Hassan ◽

Sadaf ◽

Imrana Tanvir ◽

...

Keyword(s):

Breast Carcinoma ◽

Substance P ◽

Tumor Cells ◽

Prognostic Significance ◽

Positive Staining ◽

Ki 67 ◽

Neurokinin 1 Receptor ◽

Group A ◽

Neurokinin 1 ◽

Group B

Expression and immunolocalization of Substance P (SP)/Neurokinin-1 Receptor (NK-1R) in breast carcinoma (BC) patients and its association with routine proliferative markers (ER, PR, HER2/neu, and Ki-67) were evaluated. A cross-sectional study was performed on 34 cases of BC. There were 23 cases of group A (grade III), 8 of group B (grade II), and only 3 cases of group C (grade I). All samples were then processed for SP and NK-1R immunohistochemistry for few cases. 14/23 cases (61%) of group A, 7/8 cases (88%) of group B, and 2/3 (67%) cases of group C were SP positive. Overall, strong staining (≥10% tumor cells), labeled as “+3,” was observed in 9/14 (64.2%) cases of group A and 1/8 (12.5%) cases of group B. Moderate staining labelled as “+2” (in ≥10% tumor cells) was observed in 3/14 (21.4%) cases of group A and 4/8 (50%) cases of group B. Weak positive staining “+1” was observed in only 2/14 (14.28%) cases of group A, 2/8 (25%) cases of group B, and all 2/2 (100%) cases of group C. SP and NK-1R are overexpressed in breast carcinomas, and there is significant association between the grade of tumor and their overexpression.

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