scholarly journals Specializations in filopodial membranes at points of attachment to the substrate.

1980 ◽  
Vol 87 (3) ◽  
pp. 562-568 ◽  
Author(s):  
J M Robinson ◽  
M J Karnovsky
Keyword(s):  
Cell Body ◽  
Freeze Fracture ◽  
Spatial Relationship ◽  
Cholesterol Content ◽  
Mouse Cell ◽  
Striking Difference ◽  
Functional Specialization ◽  
Monolayer Cultures ◽  
Relationship Of

A mouse cell line (LM), which grows predominantly as spindle-shaped cells with numerous filopodia, was employed in this study. These filopodial projections appear to be important as sites of attachment to the substratum in LM cells. Morphologically the filopodia are slender projections from the cell body which usually attach to the substrate at their distal ends (filopodial footpads). Freeze-fracture of monolayer cultures in situ preserves the spatial relationship of filopodial processes to that of the cell body. Examination of these freeze-fracture preparations reveals a striking difference in the density of intramembrane particles (IMP) in the filopodial-footpad plasmalemma compared with the plasmalemma of the cell body (number of IMP in footpad > cell body). Additionally, there is a marked difference in the number of filipin-sterol complexes on the cell body, compared with the filopodial footpad, implying a difference in the cholesterol content in these regions (filipin-sterol complexes in footpad < cell body). These data suggest a structural and functional specialization of the filopodial-footpad plasma membrane which may be related to cell adhesion.

scholarly journals A three-dimensional structural dissection of Drosophila polytene chromosomes.

1995 ◽  
Vol 131 (2) ◽  
pp. 279-295 ◽  
Author(s):  
Y Urata ◽  
S J Parmelee ◽  
D A Agard ◽  
J W Sedat
Keyword(s):  
Rna Polymerase Ii ◽  
Polytene Chromosome ◽  
Polytene Chromosomes ◽  
Three Dimensional ◽  
Spatial Relationship ◽  
Band Shape ◽  
Structural Details ◽  
Novel Method ◽  
Relationship Of

We have analyzed the three-dimensional structural details of Drosophila melanogaster polytene chromosome bands and interbands using three-dimensional light microscopy and a novel method of sample preparation that does not involve flattening or stretching the chromosomes. Bands have been visualized in unfixed chromosomes stained with the DNA specific dye 4,6-Diamidino-2-phenylindole (DAPI). Interbands have been visualized using fixed chromosomes that have been immunostained with an antibody to RNA polymerase II. Additionally, these structures have been analyzed using in situ hybridization with probes from specific genetic loci (Notch and white). Bands are seen to be composed of approximately 36 substructural features that measure 0.2-0.4 micron in diameter. We suggest that these substructural features are in fact longitudinal fibers made up of bundles of chromatids. Band shape can be a reproducible characteristic of a particular band and is dependent on the spatial relationship of these bundles, varying from bands with a uniform distribution of bundles to bands with a peripheral concentration of chromatin. Interbands are composed of bundles of chromatids of a similar size and number as those seen in the bands. The distribution of bundles is similar between a band and the neighboring interband, implying that there is a long range organization to the DNA that includes both the coding and the noncoding portions of genes. Finally, we note that the polytene chromosome has a circular shape when viewed in cross section, whether there are one or two homologs present.

scholarly journals A simple method for freeze-fracture of monolayer cultures.

1975 ◽  
Vol 67 (3) ◽  
pp. 904-911 ◽  
Author(s):  
T R Collins ◽  
J C Bartholomew ◽  
M Calvin
Keyword(s):  
Freeze Fracture ◽  
Silicon Monoxide ◽  
Cellular Interaction ◽  
3T3 Cells ◽  
Simple Method ◽  
Mouse Embryo Fibroblasts ◽  
Membrane Specialization ◽  
Monolayer Cultures ◽  
Growth Properties

A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to determine how areas of membrane specialization are related to the cell as a whole and to regions of cellular interaction. 3T3 cells cultured on silicon monoxide show no modification of growth properties compared to cells growing on Falcon plastic, and other cell lines also appear to grow well on this substrate.

Localization of HPV and SC35 Domains in SiHa and CaSki Cells by TSA Using Fluorescent Tyramides

1997 ◽  
Vol 3 (S2) ◽  
pp. 141-142
Author(s):  
K.E. Adler ◽  
P.T. Moen ◽  
T.J. Erickson ◽  
M.N. Bobrow
Keyword(s):  
Spatial Relationship ◽  
High Sensitivity ◽  
Single Copy ◽  
Hpv Dna ◽  
Siha Cells ◽  
Specialized Equipment ◽  
Related Proteins ◽  
Relationship Of ◽  
Antigen 5

Detection of integrated HPV DNA in SiHa and CaSki cells has been demonstrated by in situ PCR (1,2,3) and more recently TSA (4). These reports focused on high sensitivity single copy HPV detection using chromogenic techniques. Here we utilize high resolution and high sensitivity fluorescent methods of detection to analyze the spatial relationship of HPV DNA with respect to subnuclear domains enriched in splicing-related proteins, shown here by immunofluorescent detection of the SC-35 antigen (5). In situhybridization was performed using conventional reagents and the resulting probe signal amplified using fluorescent labeled tyramides (6). In contrast to techniques such as in situ PCR which require specialized procedures and equipment, detection of HPV DNA in SiHa cells was rapidly and routinely achieved using no specialized equipment. In addition, high target resolution was maintained, in contrast to in situ PCR, which is plagued by signal dispersion and difficult localization (7).

Ultrastructural evidence for mast cell-macrophage interrelationships in the dura mater of the rat: Infrequent mast cell-nerve associations

1990 ◽  
Vol 48 (3) ◽  
pp. 352-353
Author(s):  
Ruth V.W. Dimlich
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Dura Mater ◽  
Spatial Relationship ◽  
Potassium Phosphate ◽  
Plasma Extravasation ◽  
Close Spatial Relationship ◽  
Male Wistar Rats ◽  
Headache Pain ◽  
Relationship Of

Mast cells in the dura mater of the rat may play a role in cerebral pathologies including neurogenic inflammation (vasodilation; plasma extravasation) and headache pain . As has been suggested for other tissues, dural mast cells may exhibit a close spatial relationship to nerves. There has been no detailed ultrastructural description of mast cells in this tissue; therefore, the goals of this study were to provide this analysis and to determine the spatial relationship of mast cells to nerves and other components of the dura mater in the rat.Four adult anesthetized male Wistar rats (290-400 g) were fixed by perfusion through the heart with 2% glutaraldehyde and 2.8% paraformaldehyde in a potassium phosphate buffer (pH 7.4) for 30 min. The head of each rat was removed and stored in fixative for a minimum of 24 h at which time the dural coverings were removed and dissected into samples that included the middle meningeal vasculature. Samples were routinely processed and flat embedded in LX 112. Thick (1 um) sections from a minimum of 3 blocks per rat were stained with toluidine blue (0.5% aqueous).

scholarly journals Geo-Spatial Analysis of Population Density and Annual Income to Identify Large-Scale Socio-Demographic Disparities

2021 ◽  
Vol 10 (7) ◽  
pp. 432
Author(s):  
Nicolai Moos ◽  
Carsten Juergens ◽  
Andreas P. Redecker
Keyword(s):  
Population Density ◽  
Large Scale ◽  
Methodological Approach ◽  
Spatial Relationship ◽  
Postal Code ◽  
Annual Income ◽  
Choropleth Maps ◽  
Clustering Approach ◽  
Relationship Of ◽  
Insight Into

This paper describes a methodological approach that is able to analyse socio-demographic and -economic data in large-scale spatial detail. Based on the two variables, population density and annual income, one investigates the spatial relationship of these variables to identify locations of imbalance or disparities assisted by bivariate choropleth maps. The aim is to gain a deeper insight into spatial components of socioeconomic nexuses, such as the relationships between the two variables, especially for high-resolution spatial units. The used methodology is able to assist political decision-making, target group advertising in the field of geo-marketing and for the site searches of new shop locations, as well as further socioeconomic research and urban planning. The developed methodology was tested in a national case study in Germany and is easily transferrable to other countries with comparable datasets. The analysis was carried out utilising data about population density and average annual income linked to spatially referenced polygons of postal codes. These were disaggregated initially via a readapted three-class dasymetric mapping approach and allocated to large-scale city block polygons. Univariate and bivariate choropleth maps generated from the resulting datasets were then used to identify and compare spatial economic disparities for a study area in North Rhine-Westphalia (NRW), Germany. Subsequently, based on these variables, a multivariate clustering approach was conducted for a demonstration area in Dortmund. In the result, it was obvious that the spatially disaggregated data allow more detailed insight into spatial patterns of socioeconomic attributes than the coarser data related to postal code polygons.

Intrasellar Intercarotid Communicating Artery Associated with Agenesis of the Right Internal Carotid Artery: Case Report

Neurosurgery ◽  
1988 ◽  
Vol 23 (6) ◽  
pp. 770-773 ◽  
Author(s):  
Masahiko Udzura ◽  
Hiroo Kobayashi ◽  
Yoshio Taguchi ◽  
Hiroaki Sekino
Keyword(s):  
Magnetic Resonance Imaging ◽  
Carotid Artery ◽  
Internal Carotid Artery ◽  
Internal Carotid ◽  
Spatial Relationship ◽  
Resonance Imaging ◽  
Anomalous Artery ◽  
Magnetic Resonance Imaging Mri ◽  
The Right ◽  
Relationship Of

Abstract A 54-year-old man with a right hemiparesis was found to have an intrasellar intercarotid communicating artery associated with agenesis of the right internal carotid artery. Magnetic resonance imaging (MRI) studies demonstrated the spatial relationship of the anomalous artery to the surrounding structures, thus suggesting an embryonic enlargement of the capsular artery as a source of this anomalous artery.

Relationship of corneal asphericity to intraocular lens power calculations after myopic laser in situ keratomileusis

2016 ◽  
Vol 42 (5) ◽  
pp. 703-709 ◽  
Author(s):  
Yosai Mori ◽  
Kimiya Shimizu ◽  
Keiichiro Minami ◽  
Kazutaka Kamiya ◽  
Nobuyuki Shoji ◽  
...  
Keyword(s):  
Intraocular Lens ◽  
Laser In Situ Keratomileusis ◽  
Power Calculations ◽  
Intraocular Lens Power ◽  
Corneal Asphericity ◽  
Lens Power ◽  
Relationship Of

scholarly journals Correlation analysis between multifractal characteristics of regional geomorphology and development of geological disasters

2021 ◽  
Vol 25 (1) ◽  
pp. 49-55
Author(s):  
Yiying Xiong
Keyword(s):  
Correlation Analysis ◽  
Spatial Relationship ◽  
Analysis Method ◽  
Self Similarity ◽  
Geological Disasters ◽  
Geological Disaster ◽  
Correlation Analysis Method ◽  
Fractal Spectrum ◽  
Fractal Characteristics ◽  
Relationship Of

In view of the inaccuracy of the traditional correlation analysis method, this paper proposes a correlation analysis method between the multifractal characteristics of regional landforms and the development of geological disasters. Firstly, the multifractal characteristics of regional landforms are described by using the basic fractal characteristics of self-similarity or scale invariance. Then the corresponding relation table is established according to the width of the fractal spectrum and the number of landslides and hidden dangers, and the spatial relationship of geological disaster development is analyzed. Combined with the above-mentioned spatial relationship of geological disaster development and the multifractal characteristic data of regional landforms, the correlation coefficient between the two is calculated to complete the correlation analysis between the multifractal characteristics of regional geomorphology and the development of geological disasters. The experimental results show that compared with the traditional correlation analysis method, the correlation analysis results of the multifractal characteristics of regional geomorphology and the development of geological disasters are more accurate.

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