scholarly journals A CORRELATIVE STUDY BY ELECTRON AND LIGHT MICROSCOPY OF THE DEVELOPMENT OF TYPE 5 ADENOVIRUS

1960 ◽  
Vol 112 (2) ◽  
pp. 383-402 ◽  
Author(s):  
Gabriel C. Godman ◽  
Councilman Morgan ◽  
Peter M. Breitenfeld ◽  
Harry M. Rose
Keyword(s):  
Electron Microscope ◽  
Nucleic Acid ◽  
Light Microscopy ◽  
Hela Cells ◽  
Light Microscope ◽  
Protein Bodies ◽  
Correlative Study ◽  
Electron And Light Microscopy

The evolution of the intranuclear lesion produced by type 5 adenovirus in HEp-2 and HeLa cells is described as seen in the light microscope and the bodies formed in the course of the infection characterized histochemically. Some 12 hours after infection acidophilic protein bodies, without appreciable nucleic acid, first appear in the nucleus and coalesce into a network. Within or in association with this material, DNA-containing masses (viral aggregates) are formed which rapidly increase in amount and then coalesce. At the same time, a protein is produced, histochemically different from that of the acidophilic or basophilic structures mentioned, within the infected nucleus, which constitutes a matrix within which regular cytstals of a protein, (presumably non-viral) materialize. These structural and histochemical features are correlated with details which have been observed in parallel studies with the electron microscope.

Selective Staining of Acid Mucopolysaccharides By Ruthenium Red

1968 ◽  
Vol 26 ◽  
pp. 38-39
Author(s):  
J. H. Luft
Keyword(s):  
Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Osmium Tetroxide ◽  
Single Cells ◽  
Ruthenium Red ◽  
Collagen Fibers ◽  
Selective Staining ◽  
Pectic Substances ◽  
Solid Tissues

Ruthenium red is one of the few completely inorganic dyes used to stain tissues for light microscopy. This novelty is enhanced by ignorance regarding its staining mechanism. However, its continued usefulness in botany for demonstrating pectic substances attests to selectivity of some sort. Whether understood or not, histochemists continue to be grateful for small favors.Ruthenium red can also be used with the electron microscope. If single cells are exposed to ruthenium red solution, sufficient mass can be bound to produce observable density in the electron microscope. Generally, this effect is not useful with solid tissues because the contrast is wasted on the damaged cells at the block surface, with little dye diffusing more than 25-50 μ into the interior. Although these traces of ruthenium red which penetrate between and around cells are visible in the light microscope, they produce negligible contrast in the electron microscope. However, its presence can be amplified by a reaction with osmium tetroxide, probably catalytically, to be easily visible by EM. Now the density is clearly seen to be extracellular and closely associated with collagen fibers (Fig. 1).

Comparison of Larval Stored Product Beetle Mandibles by Light Microscope and Scanning Electron Microscope

1981 ◽  
Vol 64 (1) ◽  
pp. 199-224
Author(s):  
John E Kvenberg
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Morphological Characteristics ◽  
Scanning Electron ◽  
Conventional Light Microscopy

Abstract Larval stored product beetle mandibles were studied by comparing images made by scanning electron microscopy with those made by conventional light microscopy. Discussion of morphological characteristics is based on illustrations of 25 species

A Simple Light-Microscope Technique for the Examination of Cell Surfaces

1973 ◽  
Vol 13 (1) ◽  
pp. 317-319
Author(s):  
A. ROSS ◽  
I. P. GORMLEY
Keyword(s):  
Heavy Metal ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Simple Technique ◽  
Cell Surfaces ◽  
Microscope Technique ◽  
Scanning Electron

A simple technique for the examination of cell surfaces by shadowing them with a heavy metal and examining by light microscopy has been described. Stained preparations can be examined and photographed initially prior to investigation by this technique. It is suggested that this technique could be used instead of the scanning electron microscope or replica techniques where the limited resolution of the light microscope is sufficient.

scholarly journals STRUCTURE AND DEVELOPMENT OF VIRUSES OBSERVED IN THE ELECTRON MICROSCOPE

1956 ◽  
Vol 2 (3) ◽  
pp. 351-360 ◽  
Author(s):  
Councilman Morgan ◽  
Calderon Howe ◽  
Harry M. Rose ◽  
Dan H. Moore
Keyword(s):  
Electron Microscope ◽  
Electron Micrographs ◽  
Nuclear Membrane ◽  
Hela Cells ◽  
Light Microscope ◽  
Thin Sections ◽  
Viral Particles ◽  
Crystalline Aggregates ◽  
Internal Body

Representative viruses of the RI-APC group were observed with the electron microscope in thin sections of infected HeLa cells. The viral particles varied in density, were approximately 60 mµ in diameter and had a center to center spacing when close packed of about 65 mµ. Many of the less dense particles exhibited an internal body averaging 24 mµ in diameter. It was suggested that within the nucleus the virus differentiated from dense granular and reticular material and formed crystals. Disintegration of the crystals and disruption of the nuclear membrane with release of virus into the cytoplasm appeared to occur at any stage. No evidence to suggest development of the virus in the cytoplasm was obtained. It was possible to deduce the structure of the viral crystal from the electron micrographs. The viral particles are packed in a cubic body—centered lattice. Correlative histochemical observations in the light microscope which are now in progress revealed that the crystals and non-crystalline aggregates of virus were strongly Feulgen-positive.

Eye Pigment Granule Ultrastructure in Drosophila Melanogaster

1973 ◽  
Vol 31 ◽  
pp. 512-513
Author(s):  
Donald C. Sun ◽  
Richard E. Crang
Keyword(s):  
Drosophila Melanogaster ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Osmium Tetroxide ◽  
Pigment Granule ◽  
Compound Eyes ◽  
Wild Type ◽  
Transmission Electron ◽  
Structural Differences ◽  
Electron And Light Microscopy

The compound eyes of wild type Drosophila melanogaster normally contain two pigments—ommochrome (brown) and drosopterine (red). However, sepiapterine (yellow), which is a biochemical precursor of drosopterine, is present in several mutant flies instead of drosopterine—or in addition to it. Transmission electron microscope observations reported here reveal fine structural differences between the pigment granules at various times post-pupation in D. melanogaster wild type as well as the mutants sepia and clot.Eyes from pupae at 54, 72 and 90 hours post-pupation were fixed with glutaraldehyde and osmium tetroxide followed by dehydration and embedment in Epon 812. Alternate thin (50-70 nm) and thick (1-2 μ) sections from the embedded material were sectioned with an ultramicrotome for electron and light microscopy respectively.

A Correlative Study of Immunofluorescence, Electron, and Light Microscopy in Immunologically Mediated Renal Tubular Disease in Man

Nephron ◽  
1979 ◽  
Vol 23 (1) ◽  
pp. 14-22 ◽  
Author(s):  
Claudine Orfila ◽  
Joël Rakotoarivony ◽  
Dominique Durand ◽  
Jean-Michel Suc
Keyword(s):  
Light Microscopy ◽  
Correlative Study ◽  
Electron And Light Microscopy ◽  
Renal Tubular ◽  
Renal Tubular Disease

scholarly journals A CORRELATIVE STUDY BY ELECTRON AND LIGHT MICROSCOPY OF THE DEVELOPMENT OF TYPE 5 ADENOVIRUS

1960 ◽  
Vol 112 (2) ◽  
pp. 373-382 ◽  
Author(s):  
Councilman Morgan ◽  
Gabriel C. Godman ◽  
Peter M. Breitenfeld ◽  
Harry M. Rose
Keyword(s):  
Light Microscopy ◽  
Potassium Permanganate ◽  
Osmium Tetroxide ◽  
Low Density ◽  
Correlative Study ◽  
Viral Particles ◽  
Nuclear Changes ◽  
Electron And Light Microscopy ◽  
Intracellular Virus ◽  
Viral Development

Stages in the nuclear changes consequent to infection with type 5 adenovirus are shown and described. Viral development seems to be confined to the nucleus where characteristic particles are found. The shape of the intracellular virus depends upon the method of preservation employed, appearing spherical after osmium tetroxide or freezing-substitution, occasionally exhibiting angulated faces after formalin and often assuming an hexagonal profile after potassium permanganate. The non-viral crystals are encountered in zones of low density, and it is suggested that crystallization results from the accumulation of protein in these areas. An hypothesis is presented to explain why these crystals, in contrast to the insect polyhedra, contain few viral particles.

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

The Broad Applications of the Scanning Electron Microscope

1969 ◽  
Vol 27 ◽  
pp. 20-21
Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Surface Topography ◽  
Great Depth ◽  
Resolving Power ◽  
Depth Of Field ◽  
Pictorial Representations ◽  
Scanning Electron

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.

Electron Microscopy of Mycoplasma Pneumoniae

1971 ◽  
Vol 29 ◽  
pp. 258-259 ◽  
Author(s):  
E. S. Boatman ◽  
G. E. Kenny
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Growth Phase ◽  
Hela Cells ◽  
Sulfonic Acid ◽  
Gamma Globulin ◽  
Horse Serum ◽  
Morphological Aspects ◽  
The Family

Information concerning the morphology and replication of organism of the family Mycoplasmataceae remains, despite over 70 years of study, highly controversial. Due to their small size observations by light microscopy have not been rewarding. Furthermore, not only are these organisms extremely pleomorphic but their morphology also changes according to growth phase. This study deals with the morphological aspects of M. pneumoniae strain 3546 in relation to growth, interaction with HeLa cells and possible mechanisms of replication.The organisms were grown aerobically at 37°C in a soy peptone yeast dialysate medium supplemented with 12% gamma-globulin free horse serum. The medium was buffered at pH 7.3 with TES [N-tris (hyroxymethyl) methyl-2-aminoethane sulfonic acid] at 10mM concentration. The inoculum, an actively growing culture, was filtered through a 0.5 μm polycarbonate “nuclepore” filter to prevent transfer of all but the smallest aggregates. Growth was assessed at specific periods by colony counts and 800 ml samples of organisms were fixed in situ with 2.5% glutaraldehyde for 3 hrs. at 4°C. Washed cells for sectioning were post-fixed in 0.8% OSO4 in veronal-acetate buffer pH 6.1 for 1 hr. at 21°C. HeLa cells were infected with a filtered inoculum of M. pneumoniae and incubated for 9 days in Leighton tubes with coverslips. The cells were then removed and processed for electron microscopy.

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