scholarly journals THE EFFECT OF EPITHELIAL REMNANT AND WHOLE ORGAN GRAFTS OF THYMUS ON THE RECOVERY OF THYMECTOMIZED IRRADIATED MICE

1969 ◽  
Vol 129 (6) ◽  
pp. 1235-1246 ◽  
Author(s):  
Esther F. Hays
Keyword(s):  
Direct Action ◽  
Lymphoid Cells ◽  
Lymphoid Tissues ◽  
Functional Development ◽  
Reticular Cells ◽  
Microscopic Morphology ◽  
Two Parameters ◽  
A Minor ◽  
Cellular Components

Work has been presented which suggests that thymus epithelial reticular cells are not effective in restoring the microscopic morphology of lymphoid tissues and their immunologic capacities. They function in recruiting precursors of thymus lymphocytes from the host animals to produce an organ which, after it becomes architecturally normal, can reconstitute the defective host. Intact thymus grafts in situ from 10–14 days, but not for shorter periods of time, have been shown to result in a return toward normal of these two parameters. Evidence is offered to show that few dividing cellular components in the lymphoid tissue originate from the thymus remnant grafts, and that a minor cellular component is contributed by the intact grafts. These data support the concept that the structural and functional development of the lymphatic tissue in thymectomized animals is dependent on thymus lymphoid cells and/or their products, and that the epithelial-reticular cells do not have a direct action in peripheral lymphoid reconstitution.

scholarly journals THE LYMPHOID TISSUES AND IMMUNE RESPONSES OF NEONATALLY THYMECTOMIZED MICE BEARING THYMUS TISSUE IN MILLIPORE DIFFUSION CHAMBERS

1964 ◽  
Vol 119 (1) ◽  
pp. 177-194 ◽  
Author(s):  
David Osoba ◽  
J. F. A. P. Miller
Keyword(s):  
Immune Responses ◽  
Intestinal Tract ◽  
Lymphoid Cells ◽  
Lymphoid Tissues ◽  
Lymphoid Follicles ◽  
Diffusion Chambers ◽  
Wasting Syndrome ◽  
Thymus Tissue ◽  
Reticular Cells ◽  
Thymectomized Mice

Neonatally thymectomized mice were implanted intraperitoneally at 7 days of age with Millipore diffusion chambers containing either embryonic or neonatal thymus tissue. Mice which received either empty diffusion chambers or no further treatment following neonatal thymectomy served as controls. In contrast to these controls, most of the mice implanted with thymus-filled chambers gained weight satisfactorily, did not develop a wasting syndrome, and had the capacity to produce serum antibodies in response to sheep erythrocytes and to reject allogeneic skin grafts. Lymphoid follicles were present in the lymph nodes, spleen, and intestinal tract of the implanted mice but most still showed some diminution in the population of lymphocytes in both blood and tissues. Control thymectomized mice had markedly depleted lymphoid tissues and low peripheral blood lymphocyte levels. The tissue recovered after 1 to 2 months from the diffusion chambers showed only epithelial-reticular cells but no lymphoid cells. It is suggested that a humoral factor produced by the thymus epithelial-reticular complex may be responsible for endowing lymphoid cells with immunological competence.

scholarly journals IL7R⍺ is required for hematopoietic stem cell reconstitution of tissue-resident lymphoid cells

2021 ◽  
Author(s):  
Atesh K Worthington ◽  
Taylor S Cool ◽  
Donna M Poscablo ◽  
Adeel Hussaini ◽  
Anna E Beaudin ◽  
...  
Keyword(s):  
Cell Types ◽  
Lineage Tracing ◽  
Lymphoid Cells ◽  
Lymphoid Tissues ◽  
Hematopoietic Stem ◽  
Functional Roles ◽  
Reciprocal Transplants ◽  
B1a Cells

Traditional, adult-derived lymphocytes that circulate provide adaptive immunity to infection and pathogens. However, subsets of lymphoid cells are also found in non-lymphoid tissues and are called tissue-resident lymphoid cells (TLCs). TLCs encompass a wide array of cell types that span the spectrum of innate-to-adaptive immune function. Unlike traditional lymphocytes that are continuously generated from hematopoietic stem cells (HSCs), many TLCs are of fetal origin and poorly generated from adult HSCs. Here, we sought to understand the development of murine TLCs across multiple tissues and therefore probed the roles of Flk2 and IL7R⍺, two cytokine receptors with known roles in traditional lymphopoiesis. Using Flk2- and Il7r-Cre lineage tracing models, we found that peritoneal B1a cells, splenic marginal zone B (MZB) cells, lung ILC2s and regulatory T cells (Tregs) were highly labeled in both models. Despite this high labeling, highly quantitative, in vivo functional approaches showed that the loss of Flk2 minimally affected the generation of these cells in situ. In contrast, the loss of IL7R⍺, or combined deletion of Flk2 and IL7R⍺, dramatically reduced the cell numbers of B1a cells, MZBs, ILC2s, and Tregs both in situ and upon transplantation, indicating an intrinsic and more essential role for IL7Rα. Surprisingly, reciprocal transplants of WT HSCs showed that an IL7Rα-/- environment selectively impaired reconstitution of TLCs when compared to TLC numbers in situ. Taken together, our data revealed functional roles of Flk2 and IL7Rα in the establishment of tissue-resident lymphoid cells.

scholarly journals Delineating spatiotemporal and hierarchical development of human fetal innate lymphoid cells

Cell Research ◽  
2021 ◽  
Author(s):  
Chen Liu ◽  
Yandong Gong ◽  
Han Zhang ◽  
Hua Yang ◽  
Yang Zeng ◽  
...  
Keyword(s):  
Single Cell ◽  
Progenitor Cells ◽  
Fetal Liver ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells ◽  
Lymphoid Tissues ◽  
Hematopoietic Stem ◽  
Molecular Features ◽  
Lymphoid Progenitors ◽  
B Lineage

AbstractWhereas the critical roles of innate lymphoid cells (ILCs) in adult are increasingly appreciated, their developmental hierarchy in early human fetus remains largely elusive. In this study, we sorted human hematopoietic stem/progenitor cells, lymphoid progenitors, putative ILC progenitor/precursors and mature ILCs in the fetal hematopoietic, lymphoid and non-lymphoid tissues, from 8 to 12 post-conception weeks, for single-cell RNA-sequencing, followed by computational analysis and functional validation at bulk and single-cell levels. We delineated the early phase of ILC lineage commitment from hematopoietic stem/progenitor cells, which mainly occurred in fetal liver and intestine. We further unveiled interleukin-3 receptor as a surface marker for the lymphoid progenitors in fetal liver with T, B, ILC and myeloid potentials, while IL-3RA– lymphoid progenitors were predominantly B-lineage committed. Notably, we determined the heterogeneity and tissue distribution of each ILC subpopulation, revealing the proliferating characteristics shared by the precursors of each ILC subtype. Additionally, a novel unconventional ILC2 subpopulation (CRTH2– CCR9+ ILC2) was identified in fetal thymus. Taken together, our study illuminates the precise cellular and molecular features underlying the stepwise formation of human fetal ILC hierarchy with remarkable spatiotemporal heterogeneity.

Size Effects on Photocatalytic H2 Generation with CdSe/CdS Core-Shell Nanocrystals

2015 ◽  
Vol 229 (1-2) ◽  
Author(s):  
Florian Ehrat ◽  
Thomas Simon ◽  
Jacek K. Stolarczyk ◽  
Jochen Feldmann
Keyword(s):  
Hydrogen Generation ◽  
Aqueous Dispersion ◽  
Core Shell ◽  
Ni Nanoparticles ◽  
Cdse Core ◽  
Photocatalytic Hydrogen Generation ◽  
Generation Capacity ◽  
Photocatalytic H2 Generation ◽  
Two Parameters

AbstractCdSe/CdS core-shell nanocrystals with controlled CdS shell thickness and CdSe core size were synthesized for several different values of these two parameters. The particles in aqueous dispersion were in situ decorated with Ni nanoparticles and evaluated for photocatalytic hydrogen generation capacity. The highest H

scholarly journals In situ visualisation of immunoglobulin genes in normal and malignant lymphoid cells

1995 ◽  
Vol 48 (3) ◽  
pp. M158-M164 ◽  
Author(s):  
C Carvalho ◽  
M Telhada ◽  
M do Carmo-Fonseca ◽  
L Parreira
Keyword(s):  
Lymphoid Cells ◽  
Immunoglobulin Genes

In situ cytokine gene expression in early stage of virulent Newcastle disease in chickens

2021 ◽  
pp. 030098582110459
Author(s):  
Corrie Brown ◽  
Jian Zhang ◽  
Mary Pantin-Jackwood ◽  
Kiril Dimitrov ◽  
Helena Lage Ferreira ◽  
...  
Keyword(s):  
B Cells ◽  
Newcastle Disease ◽  
Early Stage ◽  
Severe Disease ◽  
Cytokine Gene Expression ◽  
Lymphoid Tissues ◽  
Tnf Α ◽  
Virulent Newcastle Disease Virus ◽  
Ifn Γ

Selected lymphoid and reproductive tissues were examined from groups of 3-week-old chickens and 62-week-old hens that were inoculated choanally and conjunctivally with 106 EID50 of a virulent Newcastle disease virus (NDV) isolate from the California 2018–2020 outbreak, and euthanized at 1, 2, and 3 days postinfection. In the 3-week-old chickens, immunohistochemistry for NDV and for T and B cell lymphocytes, as well as in situ hybridization for IL-1β, IL-6, IFN-γ, and TNF-α revealed extensive expression of IL-1β and IL-6 in lymphoid tissues, often coinciding with NDV antigen. IFN-γ was only expressed infrequently in the same lymphoid tissues, and TNF-α was rarely expressed. T-cell populations initially expanded but by day 3 their numbers were below control levels. B cells underwent a similar expansion but remained elevated in some tissues, notably spleen, cecal tonsils, and cloacal bursa. Cytokine expression in the 62-week-old hens was overall lower than in the 3-week-old birds, and there was more prolonged infiltration of both T and B cells in the older birds. The strong pro-inflammatory cytokine response in young chickens is proposed as the reason for more severe disease.

scholarly journals Current treatment of double hit and double expressor lymphoma

Hematology ◽  
2017 ◽  
Vol 2017 (1) ◽  
pp. 295-297 ◽  
Author(s):  
Patrick M. Reagan ◽  
Andrew Davies
Keyword(s):  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Total Abdominal Hysterectomy ◽  
Abdominal Hysterectomy ◽  
Current Treatment ◽  
Current Data ◽  
Lymphoid Cells ◽  
High Grade ◽  
Computed Tomography Scans

Abstract A 60-year-old female presented with abdominal pain and distension. Following computed tomography scans of the abdomen and pelvis, she was taken urgently to the operating room, with the belief that she had appendicitis with perforation. At laparotomy, the findings were consistent with an ovarian carcinoma; there was extensive infiltration of the ovary, bowel, and omental deposits. Cytoreductive surgery was performed including total abdominal hysterectomy and bilateral salpingo-oophorectomy. The final pathology, however, revealed infiltration with medium-sized atypical lymphoid cells positive for CD20, CD10, MYC, BLC2, and BCL6 by immunohistochemistry. MYC and BCL2 translocations were identified by fluorescence in situ hybridization consistent with a diagnosis of high-grade B-cell lymphoma with rearrangements of MYC and BCL2. With the current data available, what is the optimal treatment of this patient?

Gene expression profiling of chicken lymphoid cells after treatment with Lactobacillus acidophilus cellular components

2008 ◽  
Vol 32 (5) ◽  
pp. 563-574 ◽  
Author(s):  
Jennifer T. Brisbin ◽  
Huaijun Zhou ◽  
Joshua Gong ◽  
Parviz Sabour ◽  
Mohammad Reza Akbari ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Lactobacillus Acidophilus ◽  
Expression Profiling ◽  
Lymphoid Cells ◽  
Cellular Components
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