scholarly journals The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.

1976 ◽  
Vol 144 (5) ◽  
pp. 1188-1203 ◽  
Author(s):  
A E Postlethwaite ◽  
R Snyderman ◽  
A H Kang
Keyword(s):  
Connective Tissue ◽  
Peripheral Blood ◽  
Human Fibroblasts ◽  
Peripheral Blood Lymphocytes ◽  
Dermal Fibroblasts ◽  
Chemotactic Factor ◽  
Human Dermal Fibroblasts ◽  
Heat Stable

A quantitative assay that measures fibroblast chemotaxis in vitro is described. Application of this technique has revealed that peripheral blood lymphocytes stimulated by antigen or mitogen in vitro produce a factor that is chemotactic for human dermal fibroblasts. This lymphocyte-derived chemotactic factor for fibroblasts (LDCF-F) is different from the lymphokine that is chemotactic for monocytes or macrophages. Macrophages are required for the generation of LDCF-F by T lymphocytes stimulated by phytohemagglutinin. The fibroblast chemotactic factor is heat stable (56 degrees C for 30 min), trypsin sensitive, and neuraminidase resistant. LDCF-F could function to attact connective tissue fibroblasts to sites at which cell-mediated immune reactions are occurring in vivo.

EFFECT OF l-CARNITINE TREATMENT IN VIVO ON APOPTOSIS AND CERAMIDE GENERATION IN PERIPHERAL BLOOD LYMPHOCYTES FROM AIDS PATIENTS: CORRELATION WITH IN VITRO RESULTS

1996 ◽  
Vol 24 (4) ◽  
pp. 618S-618S ◽  
Author(s):  
M. Grazia Cifone ◽  
Edoardo Alesse ◽  
Luisa Di Marzio ◽  
Paola Roncaioli ◽  
Francesca Zazzeroni ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Aids Patients ◽  
Blood Lymphocytes ◽  
Ceramide Generation

Matrix Properties, Biocompatibility and Osteoplastic Potentialities of Composite Materials Based on Polylactoglycolide and Natural Coral Skeleton Granules of Various Dispersity

2013 ◽  
Vol 20 (4) ◽  
pp. 17-23
Author(s):  
N. S Sergeeva ◽  
I. K Sviridova ◽  
G. A Frank ◽  
V. A Kirsanova ◽  
S. A Akhmedova ◽  
...  
Keyword(s):  
Composite Materials ◽  
Connective Tissue ◽  
Human Fibroblasts ◽  
Bone Defects ◽  
Coral Skeleton ◽  
Matrix Properties ◽  
Natural Coral ◽  
Tibia Defect

Results of in vitro and in vivo medico5biological study of mineral-polymer composites (MPC) based on high molecular polylactoglycolide and natural A. cervicornis coral skeleton with vari5 ous dispersity (600 µm) as materials for bone defects substitution are presented. On the model of human fibroblasts in vitro it was shown that MPC were not toxic and possessed satisfactory matrix (for cells) properties. The optimum for composite size of natural coral granules made up 200-600 µm. MPC biocompatibility was shown in subcutaneous test in mice. However comparatively slow subcutaneous substitution of both polylactoglycolide and MPC on its basis by connective tissue. Study of MPC and its components’ osteoplastic potential showed that in the zone of fenestral tibia defect in rats polylactoglycolide was substituted by connective tissue. Periosteal osteogenesis that in MPC was supplemented by enchondral osteogenesis was observed around the particles of natural coral skeleton.

scholarly journals Expression of pro-inflammatory markers by human dermal fibroblasts in a three-dimensional culture model is mediated by an autocrine interleukin-1 loop

2004 ◽  
Vol 379 (2) ◽  
pp. 351-358 ◽  
Author(s):  
Daniela KESSLER-BECKER ◽  
Thomas KRIEG ◽  
Beate ECKES
Keyword(s):  
Keratinocyte Growth Factor ◽  
Interleukin 1 ◽  
Three Dimensional ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Inflammatory Processes ◽  
Cox 2 ◽  
Extracellular Matrix Interactions

In vivo, fibroblasts reside in connective tissues, with which they communicate in a reciprocal way. Such cell–extracellular matrix interactions can be studied in vitro by seeding fibroblasts in collagen lattices. Depending upon the mechanical properties of the system, fibroblasts are activated to assume defined phenotypes. In the present study, we examined a transcriptional profile of primary human dermal fibroblasts cultured in a relaxed collagen environment and found relative induction (>2-fold) of 393 out of approx. 7100 transcripts when compared with the same system under mechanical tension. Despite down-regulated proliferation and matrix synthesis, cells did not become generally quiescent, since they induced transcription of numerous other genes including matrix metalloproteinases (MMPs) and growth factors/cytokines. Of particular interest was the induction of gene transcripts encoding pro-inflammatory mediators, e.g. cyclo-oxygenase-2 (COX-2), and interleukins (ILs)-1 and -6. These are apparently regulated in a hierarchical fashion, since the addition of IL-1 receptor antagonist prevented induction of COX-2, IL-1 and IL-6, but not that of MMP-1 or keratinocyte growth factor (KGF). Our results suggest strongly that skin fibroblasts are versatile cells, which adapt to their extracellular environment by displaying specific phenotypes. One such phenotype, induced by a mechanically relaxed collagen environment, is the ‘pro-inflammatory’ fibroblast. We propose that fibroblasts that are embedded in a matrix environment can actively participate in the regulation of inflammatory processes.

scholarly journals Induction of fibroblast chemotaxis by fibronectin. Localization of the chemotactic region to a 140,000-molecular weight non-gelatin-binding fragment.

1981 ◽  
Vol 153 (2) ◽  
pp. 494-499 ◽  
Author(s):  
A E Postlethwaite ◽  
J Keski-Oja ◽  
G Balian ◽  
A H Kang
Keyword(s):  
Molecular Weight ◽  
Tissue Injury ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Human Monocytes ◽  
Directional Migration

Plasma and cell-derived fibronectin are potent chemoattractants for human dermal fibroblasts in vitro. The chemotactic property of fibronectin resides in a major 140,000-mol wt non-gelatin-binding fragment of the native molecule. Human monocytes and neutrophils do not recognize fibronectin as a chemotactic stimulus. These findings suggest that fibronectin and perhaps certain fragments of fibronectin may function in vivo as a specific chemoattractant for fibroblasts and could, therefore, induce directional migration of fibroblasts to sites of tissue injury, remodeling or morphogenesis.

scholarly journals Defect in B cell function in HTLV III/LAV positive hemophilia patients

Blood ◽  
1987 ◽  
Vol 69 (5) ◽  
pp. 1388-1393 ◽  
Author(s):  
EJ Sjamsoedin-Visser ◽  
CJ Heijnen ◽  
BJ Zegers ◽  
JW Stoop
Keyword(s):  
B Cells ◽  
Peripheral Blood ◽  
Cell Function ◽  
Cell Activity ◽  
Peripheral Blood Lymphocytes ◽  
T Helper Cell ◽  
Helper Cell ◽  
T Helper

Abstract The capacity of the peripheral blood lymphocytes (PBL) to generate an antibody response in vitro T cell-dependent antigen ovalbumin was studied in 12 severe hemophilia patients who were otherwise in good health. PBL from four of 12 patients were not capable of generating such a response after stimulation in vitro, whereas all controls were normal. This negative plaque-forming cell (PFC) response coincided with the presence of antibodies directed toward human T-lymphotropic virus III/lymphadenopathy-associated virus (HTLV-III/LAV). Only one patient with antibodies against HTLV-III/LAV had a normal PFC response. The negative PFC response was not due to a deficient T helper cell activity, nor to an excessive T suppressor cell function. However, in the peripheral blood of these four patients, the presence of activated B cells that are refractory to antigen-specific T helper cell signals and secrete specific antibodies spontaneously could be demonstrated. Most of the patients showed a hyperimmunoglobulinemia. No correlation between the T4/T8 ratio and the level of the PFC response was demonstrable. From the data obtained in these investigations we raise the hypothesis that infection with HTLV-III/LAV in hemophilia patients will lead to in vivo (pre)activation of B cells that results in unresponsiveness or decreased response to antigen-specific signals.

scholarly journals Nivalenol induced apoptosis in human peripheral blood lymphocytes in vitro and mouse peripheral blood lymphocytes in vivo

1997 ◽  
Vol 1997 (45) ◽  
pp. 33-37 ◽  
Author(s):  
Naoto YOSHINO ◽  
Mari TAKIZAWA ◽  
Hiroki OKUMURA ◽  
Tomomi IHARA ◽  
Masao SUGAMATA ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Induced Apoptosis

Effect of amiodarone on the phospholipid and lamellar body content of lymphoblasts in vitro and peripheral blood lymphocytes in vivo

1991 ◽  
Vol 41 (6-7) ◽  
pp. 1007-1013 ◽  
Author(s):  
Karl Y. Hostetler ◽  
Jerry Vande Berg ◽  
Kathy A. Aldern ◽  
Gregory T. Brophy
Keyword(s):  
Peripheral Blood ◽  
Lamellar Body ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes
Sign in / Sign up

Export Citation Format

Share Document