Matrix Properties, Biocompatibility and Osteoplastic Potentialities of Composite Materials Based on Polylactoglycolide and Natural Coral Skeleton Granules of Various Dispersity

Results of in vitro and in vivo medico5biological study of mineral-polymer composites (MPC) based on high molecular polylactoglycolide and natural A. cervicornis coral skeleton with vari5 ous dispersity (600 µm) as materials for bone defects substitution are presented. On the model of human fibroblasts in vitro it was shown that MPC were not toxic and possessed satisfactory matrix (for cells) properties. The optimum for composite size of natural coral granules made up 200-600 µm. MPC biocompatibility was shown in subcutaneous test in mice. However comparatively slow subcutaneous substitution of both polylactoglycolide and MPC on its basis by connective tissue. Study of MPC and its components’ osteoplastic potential showed that in the zone of fenestral tibia defect in rats polylactoglycolide was substituted by connective tissue. Periosteal osteogenesis that in MPC was supplemented by enchondral osteogenesis was observed around the particles of natural coral skeleton.

Download Full-text

In Vitro Evaluation of the Composite Alginate - Calcium Phosphate Materials for Prototyping Technologies in Bone Defects Substitution

N N Priorov Journal of Traumatology and Orthopedics ◽

10.32414/0869-8678-2015-1-28-34 ◽

2015 ◽

pp. 28-34 ◽

Cited By ~ 1

Author(s):

N. S. Sergeeva ◽

V. S. Komlev ◽

I. K. Sviridova ◽

V. A. Kirsanova ◽

S. A. Akhmedova ◽

...

Keyword(s):

Composite Materials ◽

Calcium Phosphate ◽

Sodium Alginate ◽

Cell Lines ◽

Mtt Assay ◽

Human Fibroblasts ◽

Bone Defects ◽

Human Osteosarcoma ◽

Matrix Properties

Porous granular composite biomaterials based on sodium alginate and calcium phosphate component: 1% sodium alginate; sodium alginate with gelatin (80/20); calcium phosphate ceramics (CHA, TCP and OCP) and composite materials alginate/CHA, alginate/TCP, alginate/OCP, alginate/gelatin (80/20)/TCP and alginate/TCP/CHA/OCP were investigated for cytotoxicity and matrix (for cells) properties in dynamics of cultivation (up to 21 days). Two adhesive cell lines - immortalized human fibroblasts (HF, strain 1608 hTERT) and human osteosarcoma (MG-63) were used for in vitro MTT assay. OCP material showed its cytotoxicity while all its composites possessed weak matrix properties. For further research on the development of prototyping technologies 3 samples of composite materials were selected: alginate/CHA, alginate/TCP and alginate/gelatin (80/20)/TCP that are characterized by cytocompatibity and the presence of either expressed or moderate matrix properties of the surface for cells.

Download Full-text

In Vitro Evaluation of the Composite Alginate - Calcium Phosphate Materials for Prototyping Technologies in Bone Defects Substitution

N.N. Priorov Journal of Traumatology and Orthopedics ◽

10.17816/vto201522128-34 ◽

2015 ◽

Vol 22 (1) ◽

pp. 28-34

Author(s):

N. S Sergeeva ◽

V. S Komlev ◽

I. K Sviridova ◽

V. A Kirsanova ◽

S. A Akhmedova ◽

...

Keyword(s):

Composite Materials ◽

Calcium Phosphate ◽

Sodium Alginate ◽

Cell Lines ◽

Mtt Assay ◽

Human Fibroblasts ◽

Bone Defects ◽

Human Osteosarcoma ◽

Matrix Properties

Download Full-text

The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.

Journal of Experimental Medicine ◽

10.1084/jem.144.5.1188 ◽

1976 ◽

Vol 144 (5) ◽

pp. 1188-1203 ◽

Cited By ~ 240

Author(s):

A E Postlethwaite ◽

R Snyderman ◽

A H Kang

Keyword(s):

Connective Tissue ◽

Peripheral Blood ◽

Human Fibroblasts ◽

Peripheral Blood Lymphocytes ◽

Dermal Fibroblasts ◽

Chemotactic Factor ◽

Human Dermal Fibroblasts ◽

Heat Stable

A quantitative assay that measures fibroblast chemotaxis in vitro is described. Application of this technique has revealed that peripheral blood lymphocytes stimulated by antigen or mitogen in vitro produce a factor that is chemotactic for human dermal fibroblasts. This lymphocyte-derived chemotactic factor for fibroblasts (LDCF-F) is different from the lymphokine that is chemotactic for monocytes or macrophages. Macrophages are required for the generation of LDCF-F by T lymphocytes stimulated by phytohemagglutinin. The fibroblast chemotactic factor is heat stable (56 degrees C for 30 min), trypsin sensitive, and neuraminidase resistant. LDCF-F could function to attact connective tissue fibroblasts to sites at which cell-mediated immune reactions are occurring in vivo.

Download Full-text

Influence of the prolonged form of lysocyme in the composition of polyurethane implant on cells and tissues in vitro and in vivo.

Morphologia ◽

10.26641/1997-9665.2021.2.16-24 ◽

2021 ◽

Vol 15 (2) ◽

pp. 16-24

Author(s):

N.A. Galatenko ◽

D.V. Kuliesh ◽

V.P. Gritsenko ◽

L.F. Narazhayko

Keyword(s):

Tissue Culture ◽

Composite Materials ◽

Connective Tissue ◽

Polymer Composite ◽

Growth And Development ◽

The Body ◽

Polymer Composite Materials ◽

Prolonged Release

Background. The development of biocompatible polymeric implant materials and providing them with biological activity is an urgent task that can significantly increase the effectiveness of such materials when used in medical practice. Objective. Investigation of the interaction of connective tissue culture matrix cells with a prolonged form of the proteolytic enzyme lysozyme as part of a polyurethane implant in vitro and study of the effect of this form on cells and tissues during implantation in experimental animals in vivo. Methods. In order to study the possible cytotoxicity of the components of polymer composite materials and the effect of lysozyme on the growth and development of the culture of fibroblastic elements, studies were performed by tissue culture in vitro. Polymeric composite materials based on polyurethaneurea without and with lysozyme were implanted into the body of white laboratory rats of the Wistar line. Cellular responses of the body after implantation were studied by light microscopy by analysis of histological micropreparations. Results. Biological studies have assessed the effect of prolonged lysozyme on cells and tissues in vitro and in vivo. Conclusion. The fibroblast tissue culture method showed the effectiveness of biologically active polyurethaneureas with prolonged release of lysozyme, which was expressed in the prolongation of the dynamics of growth and development of cellular elements in vitro. It has been shown that lysozyme in the composition of polymer composite materials had a biological effect and helped to reduce the cellular response to the implantation of polymer samples, accelerating the formation of connective tissue capsules around the implants.

Download Full-text

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053425 ◽

1982 ◽

Vol 40 ◽

pp. 142-145

Author(s):

E. J. Kollar

Keyword(s):

Connective Tissue ◽

Oral Mucosa ◽

Basal Lamina ◽

Functional Derivatives ◽

Specific Source ◽

Dental Epithelium ◽

Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Download Full-text

Antimalarial drugs—are they beneficial in rheumatic and viral diseases?—considerations in COVID-19 pandemic

Clinical Rheumatology ◽

10.1007/s10067-021-05805-5 ◽

2021 ◽

Author(s):

Bogna Grygiel-Górniak

Keyword(s):

Connective Tissue ◽

Viral Infections ◽

Current Knowledge ◽

Inflammatory Diseases ◽

Antiviral Drugs ◽

In Vivo Studies ◽

Viral Diseases ◽

Cardiac Complications

AbstractThe majority of the medical fraternity is continuously involved in finding new therapeutic schemes, including antimalarial medications (AMDs), which can be useful in combating the 2019-nCoV: coronavirus disease (COVID-19). For many decades, AMDs have been widely used in the treatment of malaria and various other anti-inflammatory diseases, particularly to treat autoimmune disorders of the connective tissue. The review comprises in vitro and in vivo studies, original studies, clinical trials, and consensus reports for the analysis, which were available in medical databases (e.g., PubMed). This manuscript summarizes the current knowledge about chloroquine (CQ)/hydroxychloroquine (HCQ) and shows the difference between their use, activity, recommendation, doses, and adverse effects on two groups of patients: those with rheumatic and viral diseases (including COVID-19). In the case of connective tissue disorders, AMDs are prescribed for a prolonged duration in small doses, and their effect is observed after few weeks, whereas in the case of viral infections, they are prescribed in larger doses for a short duration to achieve a quick saturation effect. In rheumatic diseases, AMDs are well tolerated, and their side effects are rare. However, in some viral diseases, the effect of AMDs is questionable or not so noticeable as suggested during the initial prognosis. They are mainly used as an additive therapy to antiviral drugs, but recent studies have shown that AMDs can diminish the efficacy of some antiviral drugs and may cause respiratory, kidney, liver, and cardiac complications.

Download Full-text

In Vitro and In Vivo Activities of a Triterpenoid Saponin Extract (PX-6518) from the Plant Maesa balansae against Visceral Leishmania Species

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.48.1.130-136.2004 ◽

2004 ◽

Vol 48 (1) ◽

pp. 130-136 ◽

Cited By ~ 59

Author(s):

Louis Maes ◽

Dirk Vanden Berghe ◽

Nils Germonprez ◽

Ludo Quirijnen ◽

Paul Cos ◽

...

Keyword(s):

Leishmania Donovani ◽

Human Fibroblasts ◽

Subcutaneous Administration ◽

Leishmania Species ◽

Host Cells ◽

Triterpenoid Saponin ◽

Sodium Stibogluconate ◽

Triterpenoid Saponins

ABSTRACT The in vitro and in vivo activities of a mixture of six oleane triterpene saponins, recovered from the methanolic extract of the leaves of the Vietnamese plant Maesa balansae (PX-6518), were evaluated against drug-sensitive visceral Leishmania strains. The in vitro 50% inhibitory concentration (IC50) against intracellular Leishmania infantum amastigotes was 0.04 μg/ml. The cytotoxic concentrations causing 50% cell death (CC50s) were about 1 μg/ml in murine macrophage host cells and >32 μg/ml in human fibroblasts (MRC-5 cell line). Evaluation in the Leishmania donovani BALB/c mouse model indicated that a single subcutaneous administration of 0.4 mg/kg at 1 day after infection reduced liver amastigote burdens by about 95% in all treated animals. If treatment was delayed until 14 days after infection, a dose of 1.6 mg/kg of body weight was required to maintain the same level of activity. Single 250-mg/kg doses of sodium stibogluconate (Pentostam) 1 and 14 days after infection produced comparable efficacies. A single dose of PX-6518 at 2.5 mg/kg administered 5 days before infection was still 100% effective in preventing liver infection, suggesting a particularly long residual action. Spleen and bone marrow could not be cleared by PX-6518 nor sodium stibogluconate. PX-6518 did not show activity after oral dosing at up to 200 mg/kg for 5 days. This study concludes that triterpenoid saponins from M. balansae show promising in vitro and in vivo antileishmanial potential and can be considered as new lead structures in the search for novel antileishmanial drugs.

Download Full-text

Biocompatibility and Bone Formation of Flexible, Cotton Wool-like PLGA/Calcium Phosphate Nanocomposites in Sheep

The Open Orthopaedics Journal ◽

10.2174/1874325001105010063 ◽

2011 ◽

Vol 5 (1) ◽

pp. 63-71 ◽

Cited By ~ 35

Author(s):

Oliver D Schneider ◽

Dirk Mohn ◽

Roland Fuhrer ◽

Karina Klein ◽

Käthi Kämpf ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Formation ◽

Antimicrobial Properties ◽

Drill Hole ◽

Bone Defects ◽

Long Bone ◽

Minimal Amount ◽

Cotton Wool

Background: The purpose of this preliminary study was to assess the in vivo performance of synthetic, cotton wool-like nanocomposites consisting of a biodegradable poly(lactide-co-glycolide) fibrous matrix and containing either calcium phosphate nanoparticles (PLGA/CaP 60:40) or silver doped CaP nanoparticles (PLGA/Ag-CaP 60:40). Besides its extraordinary in vitro bioactivity the latter biomaterial (0.4 wt% total silver concentration) provides additional antimicrobial properties for treating bone defects exposed to microorganisms. Materials and Methods: Both flexible artificial bone substitutes were implanted into totally 16 epiphyseal and metaphyseal drill hole defects of long bone in sheep and followed for 8 weeks. Histological and histomorphological analyses were conducted to evaluate the biocompatibility and bone formation applying a score system. The influence of silver on the in vivo performance was further investigated. Results: Semi-quantitative evaluation of histology sections showed for both implant materials an excellent biocompatibility and bone healing with no resorption in the adjacent bone. No signs of inflammation were detectable, either macroscopically or microscopically, as was evident in 5 µm plastic sections by the minimal amount of inflammatory cells. The fibrous biomaterials enabled bone formation directly in the centre of the former defect. The area fraction of new bone formation as determined histomorphometrically after 8 weeks implantation was very similar with 20.5 ± 11.2 % and 22.5 ± 9.2 % for PLGA/CaP and PLGA/Ag-CaP, respectively. Conclusions: The cotton wool-like bone substitute material is easily applicable, biocompatible and might be beneficial in minimal invasive surgery for treating bone defects.

Download Full-text