scholarly journals Induction of fibroblast chemotaxis by fibronectin. Localization of the chemotactic region to a 140,000-molecular weight non-gelatin-binding fragment.

1981 ◽  
Vol 153 (2) ◽  
pp. 494-499 ◽  
Author(s):  
A E Postlethwaite ◽  
J Keski-Oja ◽  
G Balian ◽  
A H Kang
Keyword(s):  
Molecular Weight ◽  
Tissue Injury ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Human Monocytes ◽  
Directional Migration

Plasma and cell-derived fibronectin are potent chemoattractants for human dermal fibroblasts in vitro. The chemotactic property of fibronectin resides in a major 140,000-mol wt non-gelatin-binding fragment of the native molecule. Human monocytes and neutrophils do not recognize fibronectin as a chemotactic stimulus. These findings suggest that fibronectin and perhaps certain fragments of fibronectin may function in vivo as a specific chemoattractant for fibroblasts and could, therefore, induce directional migration of fibroblasts to sites of tissue injury, remodeling or morphogenesis.

scholarly journals Expression of pro-inflammatory markers by human dermal fibroblasts in a three-dimensional culture model is mediated by an autocrine interleukin-1 loop

2004 ◽  
Vol 379 (2) ◽  
pp. 351-358 ◽  
Author(s):  
Daniela KESSLER-BECKER ◽  
Thomas KRIEG ◽  
Beate ECKES
Keyword(s):  
Keratinocyte Growth Factor ◽  
Interleukin 1 ◽  
Three Dimensional ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Inflammatory Processes ◽  
Cox 2 ◽  
Extracellular Matrix Interactions

In vivo, fibroblasts reside in connective tissues, with which they communicate in a reciprocal way. Such cell–extracellular matrix interactions can be studied in vitro by seeding fibroblasts in collagen lattices. Depending upon the mechanical properties of the system, fibroblasts are activated to assume defined phenotypes. In the present study, we examined a transcriptional profile of primary human dermal fibroblasts cultured in a relaxed collagen environment and found relative induction (>2-fold) of 393 out of approx. 7100 transcripts when compared with the same system under mechanical tension. Despite down-regulated proliferation and matrix synthesis, cells did not become generally quiescent, since they induced transcription of numerous other genes including matrix metalloproteinases (MMPs) and growth factors/cytokines. Of particular interest was the induction of gene transcripts encoding pro-inflammatory mediators, e.g. cyclo-oxygenase-2 (COX-2), and interleukins (ILs)-1 and -6. These are apparently regulated in a hierarchical fashion, since the addition of IL-1 receptor antagonist prevented induction of COX-2, IL-1 and IL-6, but not that of MMP-1 or keratinocyte growth factor (KGF). Our results suggest strongly that skin fibroblasts are versatile cells, which adapt to their extracellular environment by displaying specific phenotypes. One such phenotype, induced by a mechanically relaxed collagen environment, is the ‘pro-inflammatory’ fibroblast. We propose that fibroblasts that are embedded in a matrix environment can actively participate in the regulation of inflammatory processes.

scholarly journals The chemotactic attraction of human fibroblasts to a lymphocyte-derived factor.

1976 ◽  
Vol 144 (5) ◽  
pp. 1188-1203 ◽  
Author(s):  
A E Postlethwaite ◽  
R Snyderman ◽  
A H Kang
Keyword(s):  
Connective Tissue ◽  
Peripheral Blood ◽  
Human Fibroblasts ◽  
Peripheral Blood Lymphocytes ◽  
Dermal Fibroblasts ◽  
Chemotactic Factor ◽  
Human Dermal Fibroblasts ◽  
Heat Stable

A quantitative assay that measures fibroblast chemotaxis in vitro is described. Application of this technique has revealed that peripheral blood lymphocytes stimulated by antigen or mitogen in vitro produce a factor that is chemotactic for human dermal fibroblasts. This lymphocyte-derived chemotactic factor for fibroblasts (LDCF-F) is different from the lymphokine that is chemotactic for monocytes or macrophages. Macrophages are required for the generation of LDCF-F by T lymphocytes stimulated by phytohemagglutinin. The fibroblast chemotactic factor is heat stable (56 degrees C for 30 min), trypsin sensitive, and neuraminidase resistant. LDCF-F could function to attact connective tissue fibroblasts to sites at which cell-mediated immune reactions are occurring in vivo.

Quercetin Shows the Pharmacological Activity to Simultaneously Downregulate the Inflammatory and Fibrotic Responses to Tissue Injury in Association with its Ability to Target Multi-Kinases

Pharmacology ◽  
2018 ◽  
Vol 102 (3-4) ◽  
pp. 142-153 ◽  
Author(s):  
Ju-Yong Song ◽  
Do Vinh Truong ◽  
Beom-Seok Yang
Keyword(s):  
Signaling Pathways ◽  
Pharmacological Activity ◽  
Tissue Injury ◽  
Dermal Fibroblasts ◽  
Organ Injury ◽  
Cutaneous Wound ◽  
Raw264.7 Macrophages ◽  
Chronic Disorders

Aim: Previous studies have suggested that quercetin is effective for treating diverse chronic disorders including organ fibrosis and airway and cardiovascular disorders. To access the pharmacological background for its broad efficacy, we examined the ability of quercetin to modulate the inflammatory and fibrotic responses associated with organ injury that commonly underlie the pathogenesis of those disorders. Methods: A cutaneous wound model on rabbit ear was used for in vivo study. Quercetin was topically applied to the wounds, and the number of macrophages and myofibroblasts and the size of the hypertrophic scar formed were estimated. An in vitro study examined the ability of quercetin to inhibit cell-signaling pathways that activate RAW264.7 macrophages and primary dermal fibroblasts and the tyrosine kinase activity of discoidin domain receptor 2. Results: Quercetin reduced the population of macrophages and myofibroblasts and the scar formation in cutaneous wound healing. Quercetin suppressed the signaling pathways activating RAW264.7 macrophages and dermal fibroblasts, which is associated with its inhibition of multiple tyrosine kinases to regulate the pathways. This pharmacological activity of quercetin to simultaneously inhibit the inflammatory and fibrotic responses upon tissue damage by targeting multi-kinases could be the action mechanism to support its broad efficacy for various chronic disorders.

Wound Healing Activity of Carbonized Rice Husk

2012 ◽  
Vol 602-604 ◽  
pp. 1196-1199
Author(s):  
Nuraly Akimbekov ◽  
Zulhair A. Mansurov ◽  
J. Jandosov ◽  
Ilya E. Digel ◽  
Mathias Gossmann ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rice Husk ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Rat Skin ◽  
Cellular Behavior ◽  
Proliferation In Vitro ◽  
Wound Healing Activity

The carbonized rice husk (CRH) was evaluated for its wound healing activity in rats using excision models. In this study, the influences of CRH on wound healing in rat skin in vivo and cellular behavior of human dermal fibroblasts in vitro were investigated. The obtained results showed that the CRH treatment promoted wound epithelization in rats and exhibited moderate inhibition of cell proliferation in vitro. CRH with lanolin oil treated wounds were found to epithelize faster as compared to controls.

Fibrin-Reinforced Collagen Matrix Enhances Tissue Regeneration and Angiogenesis

2005 ◽  
Vol 288-289 ◽  
pp. 257-260
Author(s):  
Dong Lim Seol ◽  
Won Hee Jang ◽  
Sung Jae Lee ◽  
Young Il Yang
Keyword(s):  
Tissue Regeneration ◽  
Nude Mice ◽  
Functional Integration ◽  
Collagen Matrix ◽  
Dermal Fibroblasts ◽  
Surrounding Tissue ◽  
Human Dermal Fibroblasts ◽  
Collagen Matrices

The goal of this study was to investigate effects of fibrin reinforcement of collagen sponges on fibroblasts-mediated contraction and in vivo tissue regeneration, especially angiogenesis. Human dermal fibroblasts (HDFs)-populated collagen sponges reinforced with or without fibrin were cultivated via the free-floating method in vitro. They were then evaluated using xenogeneic implantation into nude mice. The HDFs-populated collagen sponges reinforced with fibrin exhibited significantly decreased HDFs-mediated contraction in vitro (p<0.05). Microvascular and cellular densities of the collagen sponges were significantly higher with fibrin than without (p<0.01). Cell ingrowths, neovascularization, and deposition of ECM matrix were more evenly distributed in the fibrin-reinforced collagen matrices. The results demonstrated that fibrin reinforcement of porous collagen sponges can reduce cell-mediated contraction in vitro while enhancing functional integration with surrounding tissue in vivo.

scholarly journals Cell-Free Fat Extract Increases Dermal Thickness by Enhancing Angiogenesis and Extracellular Matrix Production in Nude Mice

2019 ◽  
Vol 40 (8) ◽  
pp. 904-913 ◽  
Author(s):  
Yuda Xu ◽  
Mingwu Deng ◽  
Yizuo Cai ◽  
Hongjie Zheng ◽  
Xiangsheng Wang ◽  
...  
Keyword(s):  
Nude Mice ◽  
Dermal Fibroblasts ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Human Dermal Fibroblasts ◽  
Dermal Thickness ◽  
Extracellular Matrix Production ◽  
Matrix Production ◽  
Cellular Components

Abstract Background Although adipose-derived stem cells (ADSCs) and nanofat exert antiaging effects on skin, they contain cellular components that have certain limitations in clinical practice. Cell-free fat extract (Ceffe) is a fraction purified from nanofat through removal of cellular components and lipid remnants that contains various growth factors. Objectives The purpose of this study was to evaluate the effects of Ceffe on cultured human dermal fibroblasts in vitro and on the dermis of nude mice in vivo. Methods In the in vitro study, human dermal fibroblasts were cultured with Ceffe for 72 hours, followed by flow cytometry measurement of cell proliferation and cell cycle. In the in vivo study, different concentrations of Ceffe were injected into the dorsal skin of nude mice for 4 weeks. The thickness of the dermis; proliferation of cells; density of the capillary; and expressions of type I and III collagen (Col-1 and Col-3), matrix metalloproteinase-1, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-3 were measured through histologic and Western blot analyses. Results Ceffe significantly increased cell proliferation in cultured dermal fibroblasts. In the mouse skin, Ceffe significantly increased the thickness of the dermis, number of proliferating cells, density of the capillary, and expressions of Col-1 and Col-3. Conclusions Ceffe increased the dermal thickness of nude mice, possibly by enhancing angiogenesis and extracellular matrix production, and can therefore be used for skin rejuvenation.

scholarly journals Effect of Bioactive Phytochemicals from Phlomis viscosa Poiret on Wound Healing

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 609 ◽  
Author(s):  
Ludmila Yarmolinsky ◽  
Arie Budovsky ◽  
Leonid Yarmolinsky ◽  
Boris Khalfin ◽  
Vladimir Glukhman ◽  
...  
Keyword(s):  
Wound Healing ◽  
Beneficial Effect ◽  
Chronic Wounds ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
In Vivo Models ◽  
Bioactive Phytochemicals ◽  
Pro Inflammatory Cytokines

Phlomis viscosa Poiret is an evergreen shrub growing in Israel, Turkey, Lebanon, and Syria with acknowledged pro-wound healing (WH) properties. In this study, we evaluated the pro-WH potential of selected compounds found in this plant. Among the pro-WH compounds (identified by us) was a combination of three chemicals—diosmin, 1-octen-3-ol, and himachala-2,4-diene which enhanced WH significantly both in in vitro and in vivo models. The determined phytochemicals combination could be used for the treatment of chronic wounds. The effect of the extracts, diosmin, 1-octen-3-ol on the secretion of pro-inflammatory cytokines, IL-6 (A) and IL-8 (B) by human dermal fibroblasts was significant (p < 0.001). In addition, the beneficial effect of extracts of P. viscosa and its phytochemicals on WH was evidenced by inhibiting the growth of several WH delaying microorganisms.

Alginate Scaffolds with Modified Micro Pores for Tissue Engineering Applications

2015 ◽  
Vol 749 ◽  
pp. 457-460
Author(s):  
Bon Kang Gu ◽  
Sang Jun Park ◽  
Min Sup Kim ◽  
Chun Ho Kim
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Tissue Regeneration ◽  
Dermal Fibroblasts ◽  
Swelling Kinetics ◽  
Human Dermal Fibroblasts ◽  
Kinetics Analysis ◽  
Swelling Rate

In this study, we developed the porous alginate (AL) scaffolds with modified pores size and distributions to actively control tissue regeneration. An addition of 5 and 10% (v/v) butanol to AL solution was effective to control pores structures of AL scaffolds. Especially, increased amount of butanol induced that proportion of smaller pores (size of around 5~10 μm) on AL scaffolds increased. Using swelling kinetics analysis, we confirmed that micro pore modified AL scaffolds show faster swelling rate than pristine scaffolds. During in vitro study, the enhanced viability and proliferation of human dermal fibroblasts (HDFs) were observed by the pore size and distribution from micro pore modified AL scaffolds. However, AL scaffolds added 10 % butanol with excessive proportion of smaller pores induced the decreased viability of HDFs for 7 days. From our results, AL scaffolds with modified pores structures represent a potential implants to control biological in vitro and in vivo functions in a variety of tissue engineering.

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