scholarly journals Structural diversity of ABC transporters

2014 ◽  
Vol 143 (4) ◽  
pp. 419-435 ◽  
Author(s):  
Josy ter Beek ◽  
Albert Guskov ◽  
Dirk Jan Slotboom
Keyword(s):  
Abc Transporters ◽  
Transport Mechanism ◽  
Protein Complexes ◽  
Structural Characteristics ◽  
Structural Diversity ◽  
Atp Binding ◽  
Vast Array ◽  
Dependent Protein ◽  
Insight Into ◽  
Atp Binding Cassette

ATP-binding cassette (ABC) transporters form a large superfamily of ATP-dependent protein complexes that mediate transport of a vast array of substrates across membranes. The 14 currently available structures of ABC transporters have greatly advanced insight into the transport mechanism and revealed a tremendous structural diversity. Whereas the domains that hydrolyze ATP are structurally related in all ABC transporters, the membrane-embedded domains, where the substrates are translocated, adopt four different unrelated folds. Here, we review the structural characteristics of ABC transporters and discuss the implications of this structural diversity for mechanistic diversity.

scholarly journals Evolutionary Analyses of ABC Transporters of Dictyostelium discoideum

2002 ◽  
Vol 1 (4) ◽  
pp. 643-652 ◽  
Author(s):  
Christophe Anjard ◽  
William F. Loomis
Keyword(s):  
Amino Acid ◽  
Dictyostelium Discoideum ◽  
Abc Transporters ◽  
Sequence Homology ◽  
Genome Project ◽  
The Other ◽  
Atp Binding ◽  
And Function ◽  
Insight Into ◽  
Atp Binding Cassette

ABSTRACT The ABC superfamily of genes is one of the largest in the genomes of both bacteria and eukaryotes. The proteins encoded by these genes all carry a characteristic 200- to 250-amino-acid ATP-binding cassette that gives them their family name. In bacteria they are mostly involved in nutrient import, while in eukaryotes many are involved in export. Seven different families have been defined in eukaryotes based on sequence homology, domain topology, and function. While only 6 ABC genes in Dictyostelium discoideum have been studied in detail previously, sequences from the well-advanced Dictyostelium genome project have allowed us to recognize 68 members of this superfamily. They have been classified and compared to animal, plant, and fungal orthologs in order to gain some insight into the evolution of this superfamily. It appears that many of the genes inferred to have been present in the ancestor of the crown organisms duplicated extensively in some but not all phyla, while others were lost in one lineage or the other.

Growth Promotion and Increased ATP-Binding Cassette Transporters Expression by Liraglutide in Triple Negative Breast Cancer Cell Line MDA-MB-231

Drug Research ◽  
2021 ◽  
Author(s):  
Amir Shadboorestan ◽  
Parastoo Tarighi ◽  
Mahsa Koosha ◽  
Homa Faghihi ◽  
Mohammad Hossein Ghahremani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Triple Negative Breast Cancer ◽  
Abc Transporters ◽  
Triple Negative ◽  
Epithelial Mesenchymal Transition ◽  
Growth Promotion ◽  
Atp Binding ◽  
Mesenchymal Transition ◽  
Atp Binding Cassette

Background Glucagon-like petide-1 (GLP-1) agonists such as liraglutide are widely employed in type 2 diabetes due to their glucose reducing properties and small risk of hypoglycemia. Recently, it has been shown that GLP-1agonists can inhibit breast cancer cells growth. Nonetheless, concerns are remained about liraglutide tumor promoting effects as stated by population studies. Material and Methods We evaluated the effects liraglutide on proliferation of MDA-MB-231 cells by MTT assay and then ATP-binding cassette (ABC) transporters expressions assessed by Real time PCR. Statistical comparisons were made using one-way analysis of variance followed by a post hoc Dunnett test. Results Here, we report that liraglutide can stimulate the growth of highly invasive triple negative cell line MDA-MB-231; which can be attributed to AMPK-dependent epithelial-mesenchymal transition (EMT) happening in MDA-MB-231 context. Toxicity effects were only observed with concentrations far above the serum liraglutide concentration. ATP-binding cassette (ABC) transporters expressions were upregulated, indicating the possible drug resistance and increased EMT. Conclusion In conclusion, these results suggest that liraglutide should be used with caution in patients who are suffering or have the personal history of triple negative breast cancer. However, more detailed studies are required to deepen understanding of liraglutide consequences in triple negative breast cancer. ▶Graphical Abstract.

scholarly journals Correction to: ATP binding cassette (ABC) transporters: expression and clinical value in glioblastoma

2018 ◽  
Vol 138 (3) ◽  
pp. 487-487
Author(s):  
Antonin Dréan ◽  
Shai Rosenberg ◽  
François-Xavier Lejeune ◽  
Larissa Goli ◽  
Aravindan Arun Nadaradjane ◽  
...  
Keyword(s):  
Abc Transporters ◽  
Atp Binding ◽  
Clinical Value ◽  
Atp Binding Cassette

scholarly journals Expression of some ATP-binding cassette transporters in acute myeloid leukemia

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Antonella Maria Salvia ◽  
Flavia Cuviello ◽  
Sabrina Coluzzi ◽  
Roberta Nuccorini ◽  
Immacolata Attolico ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Myeloid Leukemia ◽  
Abc Transporters ◽  
Healthy Subjects ◽  
Myeloid Leukemia ◽  
Resistance Mechanisms ◽  
Atp Binding ◽  
Atp Binding Cassette Transporters ◽  
Acute Myeloid ◽  
Atp Binding Cassette

Hematopoietic cells express ATP binding cassette (ABC) transporters in relation to different degrees of differentiation. One of the known multidrug resistance mechanisms in acute myeloid leukemia (AML) is the overexpression of efflux pumps belonging to the superfamily of ABC transporters such as ABCB1, ABCG2 and ABCC1. Although several studies were carried out to correlate ABC transporters expression with drug resistance, little is known about their role as markers of diagnosis and progression of the disease. For this purpose we investigated the expression, by real-time PCR, of some ABC genes in bone marrow samples of AML patients at diagnosis and after induction therapy. At diagnosis, ABCG2 was always down-regulated, while an up regulated trend for ABCC1 was observed. After therapy the examined genes showed a different expression trend and approached the values of healthy subjects suggesting that this event could be considered as a marker of AML regression. The expression levels of some ABC transporters such as ABCC6, seems to be related to gender, age and to the presence of FLT3/ITD gene mutation.

scholarly journals Stickleback embryos use ATP-binding cassette transporters as a buffer against exposure to maternally derived cortisol

2016 ◽  
Vol 283 (1826) ◽  
pp. 20152838 ◽  
Author(s):  
Ryan T. Paitz ◽  
Syed Abbas Bukhari ◽  
Alison M. Bell
Keyword(s):  
Abc Transporters ◽  
Gasterosteus Aculeatus ◽  
Transcriptional Response ◽  
Threespine Stickleback ◽  
Egg Laying ◽  
Atp Binding ◽  
Active Efflux ◽  
Atp Binding Cassette Transporters ◽  
Stress Influences ◽  
Atp Binding Cassette

Offspring from females that experience stressful conditions during reproduction often exhibit altered phenotypes and many of these effects are thought to arise owing to increased exposure to maternal glucocorticoids. While embryos of placental vertebrates are known to regulate exposure to maternal glucocorticoids via placental steroid metabolism, much less is known about how and whether egg-laying vertebrates can control their steroid environment during embryonic development. We tested the hypothesis that threespine stickleback ( Gasterosteus aculeatus ) embryos can regulate exposure to maternal steroids via active efflux of maternal steroids from the egg. Embryos rapidly (within 72 h) cleared intact steroids, but blocking ATP-binding cassette (ABC) transporters inhibited cortisol clearance. Remarkably, this efflux of cortisol was sufficient to prevent a transcriptional response of embryos to exogenous cortisol. Taken together, these findings suggest that, much like their placental counterparts, developing fish embryos can actively regulate their exposure to maternal cortisol. These findings highlight the fact that even in egg-laying vertebrates, the realized exposure to maternal steroids is mediated by both maternal and embryonic processes and this has important implications for understanding how maternal stress influences offspring development.

scholarly journals Two B-type ATP-binding cassette (ABC) transporters localize to the plasma membrane in Thalictrum minus

2015 ◽  
Vol 32 (3) ◽  
pp. 243-247 ◽  
Author(s):  
Nobukazu Shitan ◽  
Kazuyoshi Terasaka ◽  
Hirobumi Yamamoto ◽  
Fumihiko Sato ◽  
Kazufumi Yazaki
Keyword(s):  
Plasma Membrane ◽  
Abc Transporters ◽  
Atp Binding ◽  
Thalictrum Minus ◽  
Atp Binding Cassette
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