Effect of Chloroquine on antioxidant enzymes and oxidative system on (Vigna radiata) plant tissues exposed to water stress

The present work reports the assessment of the effectiveness of a foliar-spray of salicylic acid (SA) on growth attributes, biochemical characteristics, antioxidant activities and osmolytes accumulation in wheat grown under control (100% field capacity) and water stressed (60% field capacity) conditions. The total available water (TAW), calculated for a rooting depth of 1.65 m was 8.45 inches and readily available water (RAW), considering a depletion factor of 0.55, was 4.65 inches. The water contents corresponding to 100 and 60% field capacity were 5.70 and 1.66 inches, respectively. For this purpose, seeds of two wheat cultivars (Fsd-2008 and S-24) were grown in pots subjected to water stress. Water stress at 60% field capacity markedly reduced the growth attributes, photosynthetic pigments, total soluble proteins (TSP) and total phenolic contents (TPC) compared with control. However, cv. Fsd-2008 was recorded as strongly drought-tolerant and performed better compared to cv. S-24, which was moderately drought tolerant. However, water stress enhanced the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2) and membrane electrolyte leakage (EL) and modulated the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as accumulation of ascorbic acid (AsA), proline (Pro) and glycine betaine (GB) contents. Foliar-spray with salicylic acid (SA; 0, 3 mM and 6 mM) effectively mitigated the adverse effects of water stress on both cultivars. SA application at 6 mM enhanced the shoot and root length, as well as their fresh and dry weights, and improved photosynthetic pigments. SA foliage application further enhanced the activities of antioxidant enzymes (SOD, POD, and CAT) and nonenzymatic antioxidants such as ascorbic acid and phenolics contents. However, foliar-spray of SA reduced MDA, H2O2 and membrane permeability in both cultivars under stress conditions. The results of the present study suggest that foliar-spray of salicylic acid was effective in increasing the tolerance of wheat plants under drought stress in terms of growth attributes, antioxidant defense mechanisms, accumulation of osmolytes, and by reducing membrane lipid peroxidation.

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Effects of Seed Priming on the Antioxidant Enzymes Activity of Mungbean (Vigna radiata) Seedlings

Pakistan Journal of Nutrition ◽

10.3923/pjn.2012.140.144 ◽

2012 ◽

Vol 11 (2) ◽

pp. 140-144 ◽

Cited By ~ 5

Author(s):

Adnan Umair ◽

Safdar Ali ◽

Muhammad Javed Tareen ◽

Ijaz Ali ◽

Muhammad Naveed Tareen

Keyword(s):

Antioxidant Enzymes ◽

Vigna Radiata ◽

Seed Priming ◽

Enzymes Activity

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Abscisic acid mediated differential growth responses of root and shoot of Vigna radiata (L.) Wilczek seedlings under water stress

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2017.12.016 ◽

2018 ◽

Vol 123 ◽

pp. 213-221 ◽

Cited By ~ 6

Author(s):

Satyajit Das ◽

Rup Kumar Kar

Keyword(s):

Abscisic Acid ◽

Water Stress ◽

Vigna Radiata ◽

Growth Responses ◽

Differential Growth

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Influence of Diazotrophic Bacteria on Antioxidant Enzymes and Some Biochemical Characteristics of Soybean Subjected to Water Stress

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(12)60187-7 ◽

2012 ◽

Vol 11 (11) ◽

pp. 1828-1835 ◽

Cited By ~ 5

Author(s):

Hamed Zakikhani ◽

Mohammad Reza Ardakani ◽

Farhad Rejali ◽

Majid Gholamhoseini ◽

Aydin Khodaei Joghan ◽

...

Keyword(s):

Antioxidant Enzymes ◽

Water Stress ◽

Diazotrophic Bacteria ◽

Biochemical Characteristics

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Effects of Water Stress and Potassium on Quantity Traits of Two Varieties of Mung Bean (Vigna Radiata L.)

Cercetari Agronomice in Moldova ◽

10.2478/cerce-2014-0011 ◽

2014 ◽

Vol 47 (1) ◽

pp. 107-114

Author(s):

Z. Fooladivanda ◽

M. Hassanzadehdelouei ◽

N. Zarifinia

Keyword(s):

Water Stress ◽

Grain Yield ◽

Vigna Radiata ◽

Mung Bean ◽

Dry Matter ◽

Block Design ◽

Growth And Yield ◽

Plant Responses ◽

Potassium Fertilizer ◽

The Impact

ABSTRACT Water stress is known as the major threat to reduced growth and yield of plants in arid and semi-arid regions. Potassium is one of the indicators of plant responses to water stress. To evaluate the impact of water stress and levels of potassium on yield and yield components of two varieties of mung bean (Vigna radiata) (promising lines VC6172 and Indian), an experiment in the form of split factorial, based on randomized complete block design with three replicates was conducted in 2011, at the research farm of Safi-Abad Dezfool, Iran (latitude 32°16’ N, longitude 48°26’ E and altitude 82.9 m above sea level) .Water stress in three levels: irrigation at 120 (no stress), 180 (moderate stress) and 240 (severe stress) mm evaporation from pan, were allocated to the main plots and potassium fertilizer at three levels (0, 90, 180 kg /ha) and two varieties of mung bean (promising line VC6172 and Indian) were allotted to the sub-plots. Results showed that water stress and potassium fertilizer significantly affect all traits. The highest grain yield (2093 kg /ha) was obtained from no stress treatment in the case of 180 kg /ha potassium. Total dry matter, number of pods and grain yield, were significantly different between the two varieties. The interaction between fertilizer and variety, on dry matter and grain yield and the interaction between irrigation and variety, on dry matter were significant. We conclude that use of potassium fertilizer can reduce the adverse effects of water stress.

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Drought Tolerant Enterobacter sp./Leclercia adecarboxylata Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of Vigna radiata (L.) R. Wilczek in Water Deficit Conditions

Biology ◽

10.3390/biology10111149 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1149

Author(s):

Bilal Ahmed ◽

Mohammad Shahid ◽

Asad Syed ◽

Vishnu D. Rajput ◽

Abdallah M. Elgorban ◽

...

Keyword(s):

Acetic Acid ◽

Water Stress ◽

Water Deficit ◽

Vigna Radiata ◽

Bioactive Molecules ◽

Carotenoid Content ◽

Drought Tolerant ◽

Nodule Biomass ◽

Intrinsic Water Use Efficiency ◽

Indole 3 Acetic Acid

Drought or water stress is a limiting factor that hampers the growth and yield of edible crops. Drought-tolerant plant growth-promoting rhizobacteria (PGPR) can mitigate water stress in crops by synthesizing multiple bioactive molecules. Here, strain PAB19 recovered from rhizospheric soil was biochemically and molecularly characterized, and identified as Enterobacter sp./Leclercia adecarboxylata (MT672579.1). Strain PAB19 tolerated an exceptionally high level of drought (18% PEG-6000) and produced indole-3-acetic acid (176.2 ± 5.6 µg mL−1), ACC deaminase (56.6 ± 5.0 µg mL−1), salicylic acid (42.5 ± 3.0 µg mL−1), 2,3-dihydroxy benzoic acid (DHBA) (44.3 ± 2.3 µg mL−1), exopolysaccharide (204 ± 14.7 µg mL−1), alginate (82.3 ± 6.5 µg mL−1), and solubilized tricalcium phosphate (98.3 ± 3.5 µg mL−1), in the presence of 15% polyethylene glycol. Furthermore, strain PAB19 alleviated water stress and significantly (p ≤ 0.05) improved the overall growth and biochemical attributes of Vigna radiata (L.) R. Wilczek. For instance, at 2% PEG stress, PAB19 inoculation maximally increased germination, root dry biomass, leaf carotenoid content, nodule biomass, leghaemoglobin (LHb) content, leaf water potential (ΨL), membrane stability index (MSI), and pod yield by 10%, 7%, 14%, 38%, 9%, 17%, 11%, and 11%, respectively, over un-inoculated plants. Additionally, PAB19 inoculation reduced two stressor metabolites, proline and malondialdehyde, and antioxidant enzymes (POD, SOD, CAT, and GR) levels in V. radiata foliage in water stress conditions. Following inoculation of strain PAB19 with 15% PEG in soil, stomatal conductance, intercellular CO2 concentration, transpiration rate, water vapor deficit, intrinsic water use efficiency, and photosynthetic rate were significantly improved by 12%, 8%, 42%, 10%, 9% and 16%, respectively. Rhizospheric CFU counts of PAB19 were 2.33 and 2.11 log CFU g−1 after treatment with 15% PEG solution and 8.46 and 6.67 log CFU g−1 for untreated controls at 40 and 80 DAS, respectively. Conclusively, this study suggests the potential of Enterobacter sp./L. adecarboxylata PAB19 to alleviate water stress by improving the biological and biochemical features and of V. radiata under water-deficit conditions.

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Biochemical responses of Digitaria commutata and Cenchrus ciliaris to water stress: antioxidative reactions, proline and soluble sugars accumulation

Enero-Abril 2021 - Bioagro ◽

10.51372/bioagro333.3 ◽

2021 ◽

Vol 33 (3) ◽

pp. 171-180

Author(s):

Taoufik Amari ◽

Chedly Abdelly

Keyword(s):

Water Stress ◽

Soluble Sugars ◽

Antioxidant Enzyme Activities ◽

Stress Exposure ◽

Cenchrus Ciliaris ◽

Psii Photochemistry ◽

Mda Content ◽

The Impact ◽

Quantum Yield Of Psii ◽

Oxidative System

The impact of water stress on antioxidant enzyme activities, proline, soluble sugars, and carotenoids contents found in Digitaria commutata and Cenchrus ciliaris plants was investigated. Two different watering regimes were used on plants over a period of three months. Water stress decreased total chlorophyll content in plants, but increased carotenoids content. Interestingly, no change was observed in the quantum yield of PSII photochemistry (Fv/Fm). Malondialdehyde (MDA) content increased to a higher extent in both species. Enhanced activities of all the enzymes (peroxidase, catalase, and superoxide dismutase) studied, except for catalase in the roots were observed. Proline and soluble sugars contents increased significantly following water stress exposure. No clear differences were found between both species. The results link drought tolerance of Digitaria commutata and Cenchrus ciliaris plants with better capabilities of anti-oxidative system. Additionally, it is linked to the accretion of osmoprotectants proline and soluble sugars when exposed to drought.

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