A method for determining long nucleotide sequences of double-stranded DNA is being developed. It involves (a) the synchronous digestion of the DNA from the 3' ends with EL coli exonuclease III (Exo III) followed by (b) resynthesis with labeled nucleotides and DNA polymerase. A crucial factor in the success of this method is the degree to which the enzyme digestion proceeds synchronously under proper conditions of incubation (step a). Dark field EM is used to obtain accurate measurements on the lengths and distribution of the DNA molecules before and after digestion with Exo III, while gel electrophoresis is used in parallel to obtain a mean length for these molecules. It is the measurements on a large enough sample of individual molecules by EM that provides the information on how synchronously the digestion proceeds. For length measurements, the DNA molecules were picked up on 20-30 Å thick carbon-aluminum films, using the aqueous Kleinschmidt technique and stained with 7.5 x 10-5M uranyl acetate in 90% ethanol for 3 minutes.
Abstract
In this study, the effect of zinc interlayer on the adhesion of nickel coatings reinforced with micrometric Al2O3 particles was examined. Nickel coating was applied by electroplating on EN AW - 5754 aluminium alloy using Watts bath at a concentration of 150 g/l of nickel sulphate with the addition of 50 g/l of Al2O3. The influence of zinc intermediate coating deposited in single, double and triple layers on the adhesion of nickel coating to aluminium substrate was also studied. The adhesion was measured by the thermal shock technique in accordance with PN-EN ISO 2819. The microhardness of nickel coating before and after heat treatment was additionally tested.
It was observed that the number of zinc interlayers applied does not significantly affect the adhesion of nickel which is determined by thermal shock. No defect that occurs after the test, such as delamination, blistering or peeling of the coating was registered. Microhardness of the nickel coatings depends on the heat treatment and the amount of zinc in the interlayer. For both single and double zinc interlayer, the microhardness of the nickel coating containing Al2O3 particles increased after heat treatment, but decreased when a triple zinc interlayer was applied.
Abstract
ELECTROLESS NICKEL is a nickel coating deposited by chemical reduction of nickel ions. The most widely used reducing agent is sodium hypophosphite. The thickness of the deposited coating is uniform over all areas of the work-piece that are in continuous contact with fresh plating solution. The process is applicable to a wide variety of metal and nonmetal substrates. This datasheet provides information on composition, physical properties, hardness, elasticity, and tensile properties. It also includes information on corrosion and wear resistance as well as heat treating and joining. Filing Code: Ni-332. Producer or source: Occidental Chemical Corporation.
ABSTRACTPure aluminum films are deposited under a variety of conditions to vary the crystallographic texture. After patterning and annealing at 400°C for 1 hour, electromigration tests are performed at several temperatures. Failure data are compared on the basis of t50 and standard deviation. Microstructure is quantified by transmission electron microscopy for grain size and grain size distribution and by X-ray diffraction for texture. A strong (111) texture significantly improves the electromigration lifetime and decreases the standard deviation in time to failure. This improvement correlates with both the fraction and sharpness of the (111) texture component.
ABSTRACTThe technique of picosecond electron diffraction is used to time resolve the
laser-induced melting of thin aluminum films. It is observed that under
rapid heating conditions, the long range order of the lattice subsists for
lattice temperatures well above the equilibrium point, indicative of
superheating. This superheating can be verified by directly measuring the
lattice temperature. The collapse time of the long range order is measured
and found to vary from 20 ps to several nanoseconds according to the degree
of superheating. Two interpretations of the delayed melting are offered,
based on the conventional nucleation and point defect theories. While the
nucleation theory provides an initial nucleus size and concentration for
melting to occur, the point defect theory offers a possible explanation for
how the nuclei are originally formed.