Segmentation of Tumour Region on Breast Histopathology Images for Assessment of Glandular Formation in Breast Cancer Grading
Abstract Breast cancer is the most silent killer among cancers nowadays. NHG system is widely accepted worldwide as a gold standard in providing the overall grade to breast cancer. One of the breast cancer features used in the NHG system is tubule formation. Assessment of tubule formation requires pathologist to identify tumour regions. However, colour variation on breast histopathology could influence tumour regions detection on breast histopathology images. Manual identification of tumour regions using microscope may also vary between pathologists. Thus, automatic segmentation is crucial to segment tumour regions. In this study, a simple approach of segmentation was proposed to segment tumour region on breast histopathology images. The proposed segmentation involved three stages: pre-processing, segmentation and post-processing. The proposed approach using GHE and median filter in the pre-processing stage; Otsu thresholding in the segmentation stage and; morphological operation and pixel removal in the post-processing stage was found able to segment the tumour region with average segmentation accuracy of 90.4 %.