Investigation of the cytotoxicity of silver nitrate and silver-cysteine nanocomplexes

Abstract Currently, a large number of studies are devoted to the investigation of the antitumor activity of silver nanoparticles and compounds, one of which is silver nitrate. However, silver nitrate has systemic and local toxic effects. In this work, a method was proposed for the synthesis of non-metallic complexes that do not contain toxic nitrate ions, and the cytotoxicity of silver nitrate and silver-amino acid nanocomplexes was investigated.

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Low concentration effects and different outcome in repeated reproduction tests with silver nanoparticles, silver nitrate and Folsomia candida (Collembola)

Environmental Sciences Europe ◽

10.1186/s12302-020-00413-7 ◽

2020 ◽

Vol 32 (1) ◽

Author(s):

Xin Zhang ◽

Juliane Filser

Keyword(s):

Silver Nanoparticles ◽

Silver Nitrate ◽

Reproductive Toxicity ◽

Aquatic Organisms ◽

Toxic Effects ◽

Adult Survival ◽

Reproduction Rate ◽

Folsomia Candida ◽

Concentration Effects ◽

Reproduction Tests

Abstract Background Toxicity of silver nanoparticles (AgNP) has been studied frequently due to a rise in application in various products. Various studies on AgNP toxicity with terrestrial and aquatic organisms confirmed their negative effects. In our previous experiments, strong variability was observed in the reproduction of Collembola (Folsomia candida) in different repeats. To investigate the effects of silver on the reproduction of Folsomia candida, they were exposed in laboratory-controlled conditions to AgNP and silver nitrate (AgNO3) at a concentration of 30 mg/kg dry soil for 28 days and compared to controlled individuals not exposed to silver. We repeated reproduction tests on the toxicity of silver to Folsomia candida four times throughout one year (April, July, October and January) in order to explore the temporal variability of their outcome. Results While adult survival was similar in all treatments and seasons, reproduction in the control increased from April to October. Significantly lower reproduction was found in January with only 385–424 juveniles per vessel, compared to 504–633 individuals in other months. Strong toxic effects of both silver treatments were observed in July, April and October. However, AgNP showed no toxic effects on the reproduction of F. candida in January. The relative toxicity of both substances varied between single experiments: AgNP were more toxic than AgNO3 in April and July, and less toxic in October and January. Conclusion These findings indicate that the reproduction of F. candida in the control had a significant effect on the results of the toxicology experiments. Moreover, we demonstrated the reproductive toxicity of AgNP in soil at a much lower concentration than reported thus far. Therefore, to guarantee reliability and reproducibility, we recommend to disregard any test results where the reproduction rate of F. candida in the control is significantly different from the average in the respective laboratory, even if the validity criteria of the test are met.

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Seasonal effects on the outcome of reproduction tests with silver nanoparticles, silver nitrate and the Collembola Folsomia candida

10.21203/rs.3.rs-22318/v1 ◽

2020 ◽

Author(s):

Xin Zhang ◽

Juliane Filser

Keyword(s):

Silver Nanoparticles ◽

Silver Nitrate ◽

Reproductive Toxicity ◽

Aquatic Organisms ◽

Toxic Effects ◽

Seasonal Effects ◽

Soil Conditions ◽

Folsomia Candida ◽

Different Seasons ◽

Reproduction Tests

Abstract Background Toxicity of silver nanoparticles (AgNP) is increasingly studied due to a rise in application in various products. Various studies on AgNP toxicity with terrestrial and aquatic organisms confirmed their negative effects. In our previous experiments, strong variability was observed in the reproduction of Collembola in different seasons. To investigate the effects of silver nanoparticles (AgNP) on the reproduction of Collembola in different seasons, Folsomia candida were exposed to AgNP and silver nitrate (AgNO 3 ) at a concentration of 30 mg/kg dry soil for 28 days. The reproduction tests were repeated during different seasons throughout one year in order to assess if animals’ sensitivity varied with the season. Results Significantly lower reproduction was found in the control in winter with only 101 (± 7) juveniles per adult, compared to 126-158 individuals in other seasons. Strong toxic effects (inhibition of reproduction by up to 50%) were observed during summer, spring and autumn in both treatments. However, AgNP showed no toxic effects on the reproduction of F. candida in winter. The relative toxicity of both substances varied with the seasons: AgNP were more toxic than AgNO 3 in spring and summer, and less toxic in autumn and winter. Conclusion These findings indicate that seasonal effects on the reproduction of Folsomia candida are significant. Moreover, we demonstrated the reproductive toxicity of AgNP in soil at a much lower concentration than reported thus far. These effects can mainly be attributed to soil conditions, which raises concern whether these commonly used test substrates are really protective.

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Synthesis of Silver Nanoparticles Using Tyrosine as Reductor and Capping Agent

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.840.360 ◽

2020 ◽

Vol 840 ◽

pp. 360-367

Author(s):

Desi Indriyani Saragih ◽

Devita Cahyani Varin Arifin ◽

Bambang Rusdiarso ◽

Suyanta Suyanta ◽

Sri Juari Santosa

Keyword(s):

Silver Nanoparticles ◽

Amino Acid ◽

Reaction Time ◽

Silver Nitrate ◽

Color Change ◽

Capping Agent ◽

Boiling Water Bath ◽

Round Shape ◽

Tyrosine Concentration ◽

Average Size

Synthesis and stability of silver nanoparticles (AgNPs) using tyrosine as a reducing and capping agent have been done. Synthesis of AgNPs was performed by mixing silver nitrate (AgNO3) solution as a precursor with tyrosine amino acid and heating it in a boiling water bath until characterized by the appearance of color change from colorless to yellow. Variations in pH, concentration, and reaction time affecting the formation of AgNPs were studied using UV-Vis spectrophotometry in the wavelength range of 300-700 nm as the main device. The synthesis was successfully conducted at pH 11 for 45 min with the optimum tyrosine concentration was 3 mM for 0.5 mM AgNO3. The optimum mole ratio AgNO3 0.5 mM to tyrosine 3 mM was 1:6. TEM and PSA characterizations showed that the particle was a round shape and 29.5 nm is average size, respectively.

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Low concentration effects and different outcome in repeated reproduction tests with silver nanoparticles, silver nitrate and Folsomia candida (Collembola)

10.21203/rs.3.rs-22318/v2 ◽

2020 ◽

Author(s):

Xin Zhang ◽

Juliane Filser

Keyword(s):

Silver Nanoparticles ◽

Silver Nitrate ◽

Reproductive Toxicity ◽

Aquatic Organisms ◽

Toxic Effects ◽

Adult Survival ◽

Reproduction Rate ◽

Folsomia Candida ◽

Negative Effects ◽

Concentration Effects

Abstract Background Toxicity of silver nanoparticles (AgNP) has been studied frequently due to a rise in application in various products. Various studies on AgNP toxicity with terrestrial and aquatic organisms confirmed their negative effects. In our previous experiments, strong variability was observed in the reproduction of Collembola (Folsomia candida) in different repeats. To investigate the effects of silver on the reproduction of Folsomia candida, they were exposed in laboratory controlled conditions to AgNP and silver nitrate (AgNO3) at a concentration of 30 mg/kg dry soil for 28 days and compared to controlled individuals not exposed to silver. The temporal variability of the reproduction using Folsomia candida and its sensitivity to silver was explored by repeating the tests were four times in April, July, October and January through out one year. Results While adult survival was similar in all treatments and seasons, reproduction in the control increased from April to October. Significantly lower reproduction was found in January with only 385-424 juveniles per vessel, compared to 504-633 individuals in other months. Strong toxic effects of both silver treatments were observed in July, April and October. However, AgNP showed no toxic effects on the reproduction of F. candida in January. The relative toxicity of both substances varied between single experiments: AgNP were more toxic than AgNO3 in April and July, and less toxic in October and January. Conclusion These findings indicate that the reproduction of F. candida in the control had a significant effect on the results of the toxicology experiments. Moreover, we demonstrated the reproductive toxicity of AgNP in soil at a much lower concentration than reported thus far. Therefore, to guarantee reliability and reproducibility, we recommend to disregard any test results where the reproduction rate of F. candida in the control is significantly different from the average in the respective laboratory, even if the validity criteria of the test are met.

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Synthesis and Characterization of Silver Nanoplates by a Seed-mediated Method

Communications in Physics ◽

10.15625/0868-3166/29/3/13763 ◽

2019 ◽

Vol 29 (3) ◽

Author(s):

Mai Ngọc Tuan Anh

Keyword(s):

Hydrogen Peroxide ◽

Silver Nanoparticles ◽

Ascorbic Acid ◽

Silver Nitrate ◽

Sodium Borohydride ◽

Trisodium Citrate ◽

Synthesis And Characterization ◽

Silver Nanoplates ◽

Seed Mediated

Silver nanoplates (SNPs) having different size were synthesized by a seed-mediated method. The seeds -silver nanoparticles with 4 – 6 nm diameters were synthesized first by reducing silver nitrate with sodium borohydride in the present of Trisodium Citrate and Hydrogen peroxide. Then these seeds were developed by continue reducing Ag\(^+\) ions with various amount of L-Ascorbic acid to form SNPs. Our analysis showed that the concentratrion of L-Ascorbic acid, a secondary reducing agent, played an important role to form SNPs. In addition, the size and in-plane dipole plasmon resonance wavelenght of silver nanoplates were increased when the concentration of added silver nitrate increased. The characterization of SNPs were studied by UV-Vis, FE-SEM, EDS and TEM methods.

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Comparison between the Influences of Silver Nanoparticles and Silver Nitrate on the Growth and Phytochemical Properties of Borage (Borago officinalis L.)

Current Nanoscience ◽

10.2174/1573413711309020013 ◽

2013 ◽

Vol 9 (2) ◽

pp. 241-247 ◽

Cited By ~ 4

Author(s):

Mehdi Seifsahandi ◽

Ali Sorooshzadeh

Keyword(s):

Silver Nanoparticles ◽

Silver Nitrate ◽

Borago Officinalis

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Modulation of Innate Immune Toxicity by Silver Nanoparticle Exposure and the Preventive Effects of Pterostilbene

International Journal of Molecular Sciences ◽

10.3390/ijms22052536 ◽

2021 ◽

Vol 22 (5) ◽

pp. 2536

Author(s):

Rong-Jane Chen ◽

Chiao-Ching Huang ◽

Rosita Pranata ◽

Yu-Hsuan Lee ◽

Yu-Ying Chen ◽

...

Keyword(s):

Silver Nanoparticles ◽

Good Alternative ◽

Innate Immune ◽

Toxic Effects ◽

Transgenic Zebrafish ◽

Zebrafish Model ◽

Cytokines And Chemokines ◽

Living Organisms ◽

And Function ◽

Immune Toxicity

Silver nanoparticles pose a potential risk to ecosystems and living organisms due to their widespread use in various fields and subsequent gradual release into the environment. Only a few studies have investigated the effects of silver nanoparticles (AgNPs) toxicity on immunological functions. Furthermore, these toxic effects have not been fully explored. Recent studies have indicated that zebrafish are considered a good alternative model for testing toxicity and for evaluating immunological toxicity. Therefore, the purpose of this study was to investigate the toxicity effects of AgNPs on innate immunity using a zebrafish model and to investigate whether the natural compound pterostilbene (PTE) could provide protection against AgNPs-induced immunotoxicity. Wild type and neutrophil- and macrophage-transgenic zebrafish lines were used in the experiments. The results indicated that the exposure to AgNPs induced toxic effects including death, malformation and the innate immune toxicity of zebrafish. In addition, AgNPs affect the number and function of neutrophils and macrophages. The expression of immune-related cytokines and chemokines was also affected. Notably, the addition of PTE could activate immune cells and promote their accumulation in injured areas in zebrafish, thereby reducing the damage caused by AgNPs. In conclusion, AgNPs may induce innate immune toxicity and PTE could ameliorate this toxicity.

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