Comparison of phenanthrene removal by Aspergillus niger ATC 16404 (filamentous fungi) and Pseudomonas putida KT2442 (bacteria) in enriched nutrient-liquid medium

ABSTRACTAdaptation to a changing environment is essential for the survival and propagation of sessile organisms, such as plants or fungi. Filamentous fungi commonly respond to a worsening of their growth conditions by differentiation of asexually or sexually produced spores. The formation of these specialized cell types is, however, also triggered as part of the general life cycle by hyphal age or density. Spores typically serve for dispersal and, therefore, translocation but can also act as resting states to endure times of scarcity. Eukaryotic differentiation in response to environmental and self-derived signals is commonly mediated by three-tiered mitogen-activated protein (MAP) kinase signaling cascades. Here, we report that the MAP kinase Fus3 of the black moldAspergillus niger(AngFus3) and its upstream kinase AngSte7 control vegetative spore formation and secondary metabolism. Mutants lacking these kinases are defective in conidium induction in response to hyphal density but are fully competent in starvation-induced sporulation, indicating that conidiation inA. nigeris triggered by various independent signals. In addition, the mutants exhibit an altered profile of volatile metabolites and secrete dark pigments into the growth medium, suggesting a dysregulation of the secondary metabolism. By assigning the AngFus3 MAP kinase pathway to the transduction of a potentially self-derived trigger, this work contributes to the unraveling of the intricate signaling networks controlling fungal differentiation. Moreover, our data further support earlier observations that differentiation and secondary metabolism are tightly linked in filamentous fungi.

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Functional characterization of Rho GTPases in Aspergillus niger uncovers conserved and diverged roles of Rho proteins within filamentous fungi

Molecular Microbiology ◽

10.1111/j.1365-2958.2010.07524.x ◽

2011 ◽

Vol 79 (5) ◽

pp. 1151-1167 ◽

Cited By ~ 77

Author(s):

Min Jin Kwon ◽

Mark Arentshorst ◽

Eelke D. Roos ◽

Cees A. M. J. J. van den Hondel ◽

Vera Meyer ◽

...

Keyword(s):

Aspergillus Niger ◽

Filamentous Fungi ◽

Rho Gtpases ◽

Functional Characterization ◽

Rho Proteins

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Organic Fertilizer Potential Using Aspergillus niger, Pseudomonas putida and Effective Microorganisms from Coconut Water Waste in Ponorogo, East Java - Indonesia

MATEC Web of Conferences ◽

10.1051/matecconf/201815603028 ◽

2018 ◽

Vol 156 ◽

pp. 03028

Author(s):

Raden Darmawan ◽

Sri Rachmania Juliastuti ◽

Nuniek Hendrianie ◽

Setiyo Gunawan ◽

Hakun Wirawasista Aparamarta ◽

...

Keyword(s):

Organic Matter ◽

Aspergillus Niger ◽

Pseudomonas Putida ◽

Organic Fertilizer ◽

Aeration Rate ◽

Coconut Water ◽

Effective Microorganisms ◽

Inorganic Fertilizers ◽

Liquid Fertilizer ◽

Water Waste

The abundance of coconut water as a waste of household industry activities in Ponorogo can be obtained. There is still organic matter considered to become a liquid fertilizer where it can be expected to substitute for the use of inorganic fertilizers. The research aims to determine the potential of coconut water as a liquid organic fertilizer involving three types of microorganisms, such as Aspergillus niger, Pseudomonas putida and Effective Microorganisms (EM4). The experiment was conducted using a mixed bacth reactor with 4 L/ minute of aeration rate and initial microorganism`s number of approximately 107 cells/ mL for 10 days. The results suggest that the highest reduction of C-organic and the biggest enhancement of N, P, K can be achieved by a combination of A. niger and P. putida with 29 %; 100 %, 110 %, 552%, respectively.

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Recherche de nouvelles activités saccharifiantes thermostables chez les champignons filamenteux

Canadian Journal of Microbiology ◽

10.1139/m90-108 ◽

1990 ◽

Vol 36 (9) ◽

pp. 625-630 ◽

Cited By ~ 1

Author(s):

P. Vanacker ◽

B. Bacle ◽

G. Vidal ◽

L. Lacoste

Keyword(s):

Aspergillus Niger ◽

Aspergillus Fumigatus ◽

Key Words ◽

Filamentous Fungi ◽

Half Life ◽

The Other ◽

Thermostable Enzymes ◽

Life Measure ◽

Industrial Use ◽

Better Than

We have searched for producers of a saccharifying activity with improved thermostability compared with industrial amyloglucosidases. These producers were chosen among thermophilic, thermotolerant, or even mesophilic fungi. Among the 846 isolated strains, five species (two Thermoascus spp., a member of the Aspergillus fumigatus group, and two members of the Aspergillus niger group) showed an amyloglucosidasic complex with the required property. Whereas the first three of these were thermophilic or thermotolerant strains, the latter two strengthen the idea that mesophilic strains can produce thermostable enzymes. The thermostability of the saccharifying complex of the Thermoascus spp., established with a half-life measure, was found to be far better than the other ones. The industrial use of these strains was discussed, and Thermoascus crustaceus seems to be the most advantageous one. Key words: filamentous fungi, amyloglucosidases, thermostability, Thermoascus.

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Aislamiento e identificación de microorganismos con potencial biofertilizante de suelos arroceros del distrito de riego del rio zulia, norte de santander

Respuestas ◽

10.22463/0122820x.599 ◽

2016 ◽

Vol 11 (2) ◽

pp. 5-13

Author(s):

Marilyn Tatiana Santos-Torres ◽

Marelys Tibisay Santos-Torres ◽

Diana Cárdenas-Caro

Keyword(s):

Pseudomonas Aeruginosa ◽

Aspergillus Niger ◽

Bacillus Cereus ◽

Pseudomonas Putida ◽

Bacillus Megaterium ◽

Trichoderma Sp ◽

Del Rio

Se evalúo la población microbiana de suelos cultivados con arroz en algunas zonas del Distrito de Riego del Río Zulia, Norte de Santander, Colombia, y suelos no cultivados con arroz. Se encontró que los suelos cultivados con arroz fueron significativamente baios en poblaciones de hongos, bacterias y actinomicetos, comparados con los suelos no cultivados. Esta situación se debe posiblemente al excesivo laboreo y aplicación de pesticidas y al monocultivo al que se han sometido estos suelos arroceros por varios años. Por lo tanto, con el uso de microorganismos biofertilizantes, aislados en suelos de zonas con similares condiciones agroecológicas, favorecerá la adaptación de éstos microorganismos en la rizósfera y promoverán el reestablecimiento del equilibrio biológico e incremento de la actividad biótica de los suelos, meiorando la calidad y productividad de los cultivos. Un total de 43 bacterias y 4 hongos fueron aislados en diferentes suelos de esta zona de las cuales fueron identificados 25 microorganismos. Según los métodos de aislamiento, las bacterias se dividieron en dos grupos: diazotróficas, especies del género Azotobacter sp. Azospirillum sp. y Beiierinclia sp. y las solubilizadoras de fosfato inorgánico como, Bacillus megaterium, Bacillus cereus, Pseudomonas putida y Pseudomonas aeruginosa. Para la identificación de hongos se evaluaron las características micro y macroscópicas en el medio agar Czapecl siguiendo las claves fúngicas de Samson, Hoel<stra y Oorsehot (1981) que permitió reconocer especies como Trichoderma sp., Aspergillus niger, Penicillium pupurogenum y Penicillium pinophyllum.Palabras Clave: Díazofrófícas; fosfatosolubílízadores; bioferrfílízantes; banco de cepas.

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The Cultivation Method Affects the Transcriptomic Response of Aspergillus niger to Growth on Sugar Beet Pulp

Microbiology Spectrum ◽

10.1128/spectrum.01064-21 ◽

2021 ◽

Author(s):

Sandra Garrigues ◽

Roland S. Kun ◽

Mao Peng ◽

Birgit S. Gruben ◽

Isabelle Benoit Gelber ◽

...

Keyword(s):

Aspergillus Niger ◽

Sugar Beet ◽

Filamentous Fungi ◽

Scientific Community ◽

Nitrogen Sources ◽

Submerged Cultures ◽

Transcriptomic Response ◽

Cultivation Method ◽

Beet Pulp ◽

Natural Response

Understanding the interaction between filamentous fungi and their natural and biotechnological environments has been of great interest for the scientific community. Submerged cultures are preferred over solid cultures at a laboratory scale to study the natural response of fungi to different stimuli found in nature (e.g., carbon/nitrogen sources, pH).

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Microbial Transformations of 3-methoxyflavone by the Strains of Aspergillus niger

Polish Journal of Microbiology ◽

10.33073/pjm-2014-016 ◽

2014 ◽

Vol 63 (1) ◽

pp. 111-114 ◽

Cited By ~ 10

Author(s):

EDYTA KOSTRZEWA-SUSŁOW ◽

MONIKA DYMARSKA ◽

TOMASZ JANECZKO

Keyword(s):

Aspergillus Niger ◽

Filamentous Fungi ◽

Wild Strain ◽

Microbial Transformation ◽

Microbial Transformations

Microbial transformation of 3-methoxyflavone into 3'-hydroxyflavon-3-yloxymethyl myristate was presented. Six filamentous fungi were used as biocatalysts: a wild strain of Aspergillus niger KB, its four UV mutants (A. niger MB, SBP, SBJ, 13/5) and the strain of Penicillium chermesinum 113. The highest yields were observed for the strains of A. niger KB and A. niger SBP (69.8% and 63.1%, respectively).

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Basic interactions of Aspergillus niger with Se(IV)

Nova Biotechnologica et Chimica ◽

10.36547/nbc.1219 ◽

2021 ◽

Vol 9 (2) ◽

pp. 141-145

Author(s):

Martin Urík ◽

Jaroslav Ševc ◽

Pavol Littera ◽

Marek Kolenčík ◽

Slavomír Čerňanský

Keyword(s):

Aspergillus Niger ◽

Liquid Medium ◽

Filamentous Fungus ◽

Indoor Environment ◽

Fungal Growth ◽

Fungal Strain ◽

Aerobic Conditions ◽

Negligible Amount ◽

Total Selenium ◽

Toxicity Effects

Filamentous fungus Aspergillus niger is commonly found on decaying vegetation or in indoor environment and has a number of uses, including application in bioremediation. Hence, the basic interactions of this common mould with selenite were studied, including biovolatilization, bioaccumulation and toxicity effects of selenite on fungal growth. The fungal strain, originally isolated from noncontaminated soil, was cultivated under aerobic conditions on liquid cultivation media with concentration of Se(IV) 19 or 27 mg.l-1 during 25 days. The fungal growth in the presence of selenite was not inhibited when compared to control, only the sporulation was reduced. The concentration of Se(IV) in liquid medium decreased rapidly within first ten days to 1 mg.l-1. However, according to results from the 25th day of cultivation, the concentration of total selenium in medium did not change significantly and only negligible amount of selenium (less then 1%) was bioaccumulated. That indicates some biotransformation of selenite into other selenium species. During the cultivation, up to 21% of total amount of selenium was transformed into volatile derivatives (biovolatilization) by filamentous fungus A. niger.

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Biodegradation of Acrylic-Based Drilling Reagents

Safety in Technosphere ◽

10.12737/137 ◽

2012 ◽

Vol 1 (4) ◽

pp. 26-29

Author(s):

Барахнина ◽

V. Barakhnina

Keyword(s):

Pseudomonas Putida ◽

Liquid Medium ◽

Rhodococcus Erythropolis ◽

Inhibitory Effect ◽

Biological Stability ◽

Fusarium Sp

As a result of biodegradation studies of the most common drilling acrylic-based reagents in the liquid medium by microorganisms Pseudomonas putida VKM 1742 D, Rhodococcus erythropolis AS 1339 D, Fusarium sp. Number 56 in the form of monocultures and associations in a 1:1:1 ratio revealed a significant biological stability of drilling additives IKSTABL, DK-DRILL A-1, CYPAN compared to GIVPAN-N and LAKRIS-20B. The evaluation of phytotoxicity of drilling additives in studied derivatives showed that the inhibitory effect of polymers decreases with their biodegradation, and the accumulated products are non-phytotoxic.

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