The effectiveness of banana tuber and goat rumen as bio activator of liquid biopesticide fertilizers

Microorganisms, particularly bacteria, in the ruminant's rumen are valuable genetic resources that many scientists interested in. In recent years, the application of next-generation sequencing technologies allows direct decoding an extracted DNA metagenome in each ecological community without culture, increasing the efficiency of exploiting interested genes. Notably, the quantity and quality of extracted DNA play an important role in getting a reliable metagenome database. In this study, DNA metagenome from goat rumen fluid was extracted by five different methods RBB (repeated bead beating plus column), RBBC (repeated bead beating), PSP1, PSP2 (PSP®Spin Stool DNA Kit, protocol 1, 2, Germany) và QIA (QIAamp® DNA Stool Mini Kit, Germany). The results showed that DNA metagenome obtained by all methods had A260/280 greater than 1.8. DNA extracted by the RBB method had high DNA concentration but low A260/230 values (less than 1.4) and still contained Taq polymerase inhibitor. After purifying by QIA column, A260/230 values of RBB-extracted DNA significantly increased up to 2.0 and Taq polymerase inhibitor in samples were removed. However, the concentrations decreased by 57% that nearly equivalent to concentration of DNA metagenome obtained by QIA. The method using PSP®Spin Stool DNA kit produced the highest DNA concentrations (from 149.7 to 195.5 ng/µl) with A260/280 ratios of 1.9 and A260/230 ratios of 1.8 to 1.9. Morever, this method was able to remove polymerase inhibitor and be performed on short time. Therefore, the PSP®Spin Stool DNA kit is a suitable method for DNA metagenome extraction of bacteria from goat rumen. DNA obtained by this method fulfilled all criteria about quality and concentration for sequencing by next-generation sequencing Illumina.

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Defaunation effects of medium-chain fatty acids and their derivatives on goat rumen protozoa.

The Journal of General and Applied Microbiology ◽

10.2323/jgam.37.439 ◽

1991 ◽

Vol 37 (5) ◽

pp. 439-445 ◽

Cited By ~ 42

Author(s):

MITSUTO MATSUMOTO ◽

TAKERU KOBAYASHI ◽

AKIO TAKENAKA ◽

HISAO ITABASHI

Keyword(s):

Fatty Acids ◽

Medium Chain ◽

Medium Chain Fatty Acids ◽

Rumen Protozoa ◽

Goat Rumen ◽

Chain Fatty Acids

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Nutrient digestibility, Haematological indices and carcass measurements of rabbits fed graded levels of goat rumen content

Global Journal of Agricultural Sciences ◽

10.4314/gjass.v4i1.2241 ◽

2005 ◽

Vol 4 (1) ◽

Cited By ~ 1

Author(s):

G Mohammed ◽

JU Igwebuike ◽

ID Kwari

Keyword(s):

Nutrient Digestibility ◽

Rumen Content ◽

Haematological Indices ◽

Goat Rumen

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Utilization of Blood Acetate and Butyrate by the Isolated, Perfused Goat Rumen

Journal of Dairy Science ◽

10.3168/jds.s0022-0302(65)88201-7 ◽

1965 ◽

Vol 48 (2) ◽

pp. 228-233 ◽

Cited By ~ 3

Author(s):

S.L. Spahr ◽

E.M. Kesler ◽

R.J. Flipse

Keyword(s):

Goat Rumen

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Molecular cloning, recombinant protein expression, tissue distribution and functional analysis of a new c-type lysozyme from Lezhi black goat rumen

Canadian Journal of Animal Science ◽

10.4141/cjas2012-150 ◽

2014 ◽

Vol 94 (1) ◽

pp. 27-34 ◽

Cited By ~ 1

Author(s):

Peng Zhang ◽

Yong Wang ◽

Mingfeng Jiang ◽

Lianlian Zhu ◽

Jianbo Li ◽

...

Keyword(s):

Functional Analysis ◽

Amino Acid ◽

Recombinant Protein ◽

Protein Expression ◽

Tissue Distribution ◽

Molecular Mass ◽

Molecular Cloning ◽

Recombinant Protein Expression ◽

Black Goat ◽

Goat Rumen

Zhang, P., Wang, Y., Jiang, M., Zhu, L., Li, J., Luo, M., Ren, H. and Liu, L. 2014. Molecular cloning, recombinant protein expression, tissue distribution and functional analysis of a new c-type lysozyme from Lezhi black goat rumen. Can. J. Anim. Sci. 94: 27–34. Three major distinct types of lysozymes have been identified in the animal kingdom and most lysozymes cloned from ruminants belong to the chicken-type (c-type). In this study, a new c-type lysozyme gene, named LZRLyz, was cloned and sequenced from the Lezhi black goat rumen. The LZRLyz cDNA has a 444 bp open reading frame (ORF) encoding a 147 amino acid polypeptide. The encoded polypeptide is predicted to have an 18 amino acid signal peptide, and a 129 amino acid mature protein with an isoelectric point (pI) of 6.08. The LZRLyz amino acid sequence shares 70.27% identity with the Capra hircus blood lysozyme and is grouped with other ruminants c-type lysozymes using the phylogenetic tree estimated by Neighbor-Jointing method. The recombinant expressed LZRLyz protein (pET-rLZR) shows a molecular mass of ∼33 kDa, which is consistent with the predicted fusion protein molecular mass and shows antimicrobial activity. Quantitative real-time RT-PCR analyses revealed that LZRLyz transcripts are expressed in all tested tissues with the predominant expression being observed in rumen and the weakest one in spleen. Results of this study suggest that the LZRLyz gene represents a new c-type lysozyme gene that likely functions in Lezhi black goat host immunity and digestive systems.

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Molecular cloning and characterization of a novel SGNH arylesterase from the goat rumen contents

Applied Microbiology and Biotechnology ◽

10.1007/s00253-011-3289-0 ◽

2011 ◽

Vol 91 (6) ◽

pp. 1561-1570 ◽

Cited By ~ 7

Author(s):

Guozeng Wang ◽

Kun Meng ◽

Huiying Luo ◽

Yaru Wang ◽

Huoqing Huang ◽

...

Keyword(s):

Molecular Cloning ◽

Goat Rumen

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In vitro fermentation of browse species using goat rumen fluid in relation to browse polyphenol content and composition

Animal Feed Science and Technology ◽

10.1016/j.anifeedsci.2017.05.021 ◽

2017 ◽

Vol 231 ◽

pp. 1-11 ◽

Cited By ~ 5

Author(s):

G. Mengistu ◽

M. Karonen ◽

J.-P. Salminen ◽

W.H. Hendriks ◽

W.F. Pellikaan

Keyword(s):

Rumen Fluid ◽

Polyphenol Content ◽

In Vitro Fermentation ◽

Browse Species ◽

Goat Rumen

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Anaerobic biodegradation of cassava wastewater under different temperatures and inoculums

Comunicata Scientiae ◽

10.14295/cs.v10i1.3009 ◽

2019 ◽

Vol 10 (1) ◽

pp. 65-76

Author(s):

Miriam Cleide Cavalcante de Amorim ◽

Paula Tereza De Souza Silva ◽

Patricia Silva Barbosa ◽

Nayara Evelyn Montefusco

Keyword(s):

Anaerobic Digestion ◽

Methane Production ◽

Semiarid Region ◽

Anaerobic Sludge ◽

Bovine Rumen ◽

Different Temperatures ◽

Influence Of Temperature On ◽

Cassava Wastewater ◽

Kinetics Of ◽

Goat Rumen

The production of starch generates, as a by-product, the cassava wastewater (manipueira), which can be treated by anaerobic digestion to provide biogas and minimize its polluting potential. The most commonly utilized biomass in the anaerobic digestion is the anaerobic sludge. The literature presents, as an alternative to sludge, bovine manure and ruminal fluids, being scarce the studies with the cassava wastewater. This research evaluated the influence of temperature on the microbial ability of cattle and goat rumen in anaerobically biodegrading the manipueira in substitution to the anaerobic sludge. The cattle and goat rumen specific methanogenic activities (SMA) were compared with that of the anaerobic sludge. Subsequently, by using the inoculum which had the best SMA results, cassava wastewater biodegradability tests were performed, investigating the kinetics of the organic matter removal and methane production at 32 ° C and 39 ° C. The bovine rumen presented better results in the SMA (0,315 g COD-CH4 g VSS.d-1) and methane production (1,026 mL). The temperature of 32 °C did not influence the activity of bovine ruminal inoculum as the kinetics of the biodegradation of the manipueira did not differ for the evaluated temperatures (0.1799 d-1 at 32°C and 0.1781 d-1 at 39°C). Bovine rumen achieved glucose reduction of 76% and 80% and methane yield of 77% and 79% for the tests at 32°C and 39°C, respectively. It is inferred that this type of inoculum might be used in reactors of anaerobic digestion processes for the treatment of the cassava wastewater at the ambient temperature of the semiarid region.

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