scholarly journals Preservation of bovine semen using egg yolk substituted with extract guava in water from yellow coconut on the semen quality and fertility of Bali cattle

2020 ◽  
Vol 454 ◽  
pp. 012071
Author(s):  
A Marawali ◽  
M S Abdullah ◽  
Jalaludin ◽  
W M Nalley ◽  
T M Hine ◽  
...  
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Bovine Semen ◽  
Bali Cattle

scholarly journals Evaluation of Bali Cattle Semen Quality During Cryopreservation with Coconut Water-Based Extenders

2021 ◽  
Vol 10 (4) ◽  
pp. 329-334
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Coconut Water ◽  
Computer Assisted ◽  
Sperm Analysis ◽  
Progressive Motility ◽  
Different Types ◽  
Before And After ◽  
Computer Assisted Sperm Analysis ◽  
Bali Cattle

The aim of this research was to evaluate the Bali cattle semen quality during cryopreservation with different types of extenders in term of live, total motility, progressive motility, and abnormality of post-thawed bull sperms. The treatments were AndroMed® (T0), Tris-based egg yolk diluent (T1), Tris-based coconut water diluent (T2), Coconut water egg yolk diluent (T3). Bulls’ semen was collected from two adult Bali cattle maintained at the semen production facility at Bali Artificial Insemination Center, Tabanan Bali. The age of the bulls were 6 years old. Sperm live, total motility, progressive motility, and abnormality were analyzed with computer assisted sperm analysis (CASA) post-dilution, before and after thawed. The study was replicated five times, and data were analyzed using analysis of variance (ANOVA). The results showed that AndroMed ® and Tris-based egg yolk had significantly higher sperm live, total motility, progressive motility and abnormalities of spermatozoa for post-dilution, after equilibration and post thawed than Tris egg yolk coconut water and Coconut water egg yolk diluent. It was concluded that AndroMed® and Tris-based egg yolk can be considered as the best suitable extender for Bali cattle sperm cryopreservation. Coconut water had a deleterious effect when supplemented with 20% in tris and egg yolk.

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

THE USE OF GLYCEROL FOR THE CRYOPRESERVATION OF INCONNU’S SPERM

2017 ◽  
pp. 117-121
Author(s):  
Amina Kumarovna Karamuldaeva ◽  
Andrey Mikhailovich Tikhomirov
Keyword(s):  
Semen Quality ◽  
Life Time ◽  
Egg Yolk ◽  
Maximum Activity ◽  
Equilibration Time ◽  
Glycerol Concentration ◽  
Scientific Center ◽  
Spawning Period ◽  
Base Solution ◽  
Academy Of Sciences

The article studies the possibility to use glycerol as cryoprotectant, instead of dimethylsulfoxide for cryopreservation of sperm of inconnu ( Stenodus leucichthys Gueldenstaedtii, 1772). Investigations were carried out from 2015 to 2016 in the laboratory of the Southern Scientific Center, Russian Academy of Sciences, on the basis of the Astrakhan State Technical University. The material collected on the Alexander sturgeon hatcheries (the Astrakhan region) in the spawning period. Native sperm of 6 male inconnu species was used as a control means. The semen quality was determined in terms of moving activity (life time) of sperm after its activation by water. As the cryoprotectant there were used: base solution - 80%, sucrose - 1.71 g/l, mannite - 0.98 g/l, yolk - 10%, dimethylsulfoxide - 10% and base solution - 87%, sucrose - 1.71% g/l, mannite - 0.98 g/l, yolk - 10%, glycerol - 3 variants: 3; 5 and 10%. In order to provide the most complete penetration of cryoprotectants into the cells there were used electrostimulation of cell membranes. Equilibration time was 5 and 15 minutes. Thawing semen was performed in a water bath at a temperature of 38-40°C. For removing protectors from cells there was chosen a saline solution (0.7% NaCl) as isotonic solution. In tests using dimethylsulfoxide life activity of sex cells was 2 times lower than in tests with glycerol: 78 and 186.2 s at the end of equilibration and 52.3 and 128.9 s after thawing. Sperm showed maximum activity under 5% glycerol concentration during equilibration - 15 min. Concentration of 3% was insufficient, concentration of 10% was excessive, as it suppressed activity of sperm. Egg yolk which coagulated together with glycerol, making difficulty for observing, had to be excluded from the composition of cryoprotectant.

104 COMPARING DIFFERENT LEVELS OF OSMOLALITY OF SUCROSE EXTENDER ON THE VIABILITY OF SPERMATOZOA IN BACTRIAN CAMEL (CAMELUS BACTRIANUS)

2006 ◽  
Vol 18 (2) ◽  
pp. 160 ◽  
Author(s):  
S. Mosaferi ◽  
A. Niasari-Naslaji ◽  
N. Bahmani ◽  
A. A. Gharahdaghi ◽  
A. Abarghani ◽  
...  
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Bactrian Camel ◽  
Dromedary Camel ◽  
Camelus Bactrianus ◽  
Semen Collection ◽  
History Of ◽  
Different Levels ◽  
Artificial Vagina

Disaccharides have been used as an extender for dromedary camel semen (Bravo et al. 2000 Anim. Reprod. Sci. 62, 173-193). More recently we have investigated the effect of different concentrations of lactose extender on the viability of Bactrian camel spermatozoa (Mosafer et al. 2005 Reprod. Fertil. Dev. 17, 197). Considering the osmolality (316.1 � 1.48 mOsm/kg) and pH (7.4 � 0.03) of Bactrian camel semen (Mosaferi et al. 2005 Theriogenology 63, 92-101), the objective of this study was to investigate the effect of osmolality of sucrose extender on the viability of Bactrian camel spermatozoa. Sucrose at the concentrations of 9, 10, 11, 12, and 13% with osmolalities of 292, 331, 356, 386, and 410 mOsm/kg, respectively, were prepared. All extenders contained 20% egg yolk and antibiotics, with pH adjusted to 6.9. Semen was collected from camels with a sound history of semen quality and fertility (n = 3) using a modified artificial vagina and divided into different treatments after mechanical reduction of semen viscosity (3). Progressive forward motility of spermatozoa was examined at the time of semen collection and at 4, 12, and 24 h after incubation at 4�C. Data were analyzed using the GLM procedure in SAS/STAT after arcsin transformation. At the time of semen dilution, the progressive forward motility of spermatozoa was greater at osmolality of 331 (23%) compared with 292 (1%), 386 (6%), and 410 (3.5%) mOsm/kg (P < 0.05). No progressive forward motility of spermatozoa was noticed after 4 h incubation at 4�C at osmolalities of 292, 386, and 410 mOsm/kg. At this time, a significant decrease (P < 0.05) of progressive forward motility occurred at osmolalities of 331 (4%) and 356 (0.5%) compared with that of the time of dilution. After 12 and 24 h incubation at 4�C, no progressive forward motility of spermatozoa was detected in any of these extenders. In conclusion, 10% sucrose (331 mOsm/kg) at the adjusted pH of 6.9 was the most suitable concentration of this disaccharide for preserving Bactrian camel semen for less than 4 h under chilled conditions.

Effects of Fescue Toxicosis on Bovine Semen Quality.

2012 ◽  
Vol 87 (Suppl_1) ◽  
pp. 429-429
Author(s):  
Heather M. Stowe ◽  
Maggie C. Miller ◽  
Samantha M. Calcatera ◽  
Matthew G. Burns ◽  
John G. Andrae ◽  
...  
Keyword(s):  
Semen Quality ◽  
Fescue Toxicosis ◽  
Bovine Semen

scholarly journals Cryopreservation of boar semen

2020 ◽  
Vol 65 (No. 4) ◽  
pp. 115-123
Author(s):  
Marija Jovičić ◽  
Eva Chmelíková ◽  
Markéta Sedmíková
Keyword(s):  
Animal Species ◽  
Semen Quality ◽  
Egg Yolk ◽  
High Rate ◽  
Freezing And Thawing ◽  
Long Term Storage ◽  
Boar Semen ◽  
Boar Spermatozoa ◽  
Term Storage

Sperm cryopreservation is the best technology for long-term storage of the semen. However, the damage of boar spermatozoa by cryopreservation is more severe than in other animal species and a standardized freezing protocol for efficient cryopreservation has not been established yet. Semen quality and freezability vary greatly between breeds as well as between individual boars and even the season. Boar spermatozoa are sensitive to low temperatures; they sustain damage and a high rate of mortality and freezing/thawing the boar semen may strongly impair the sperm function and decrease the semen quality. The freezability of boar semen can be influenced by a cryopreservation procedure, and also by using various additives to freezing and thawing extenders such as antioxidants. In order to obtain acceptable results after thawing the boar semen, it is necessary to combine an optimal amount of additives (glycerol, egg yolk, sugars, antioxidants), cooling and warming velocities.

scholarly journals Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 653
Author(s):  
Maja Zakošek Pipan ◽  
Margret L. Casal ◽  
Nataša Šterbenc ◽  
Irma Virant Klun ◽  
Janko Mrkun
Keyword(s):  
Semen Quality ◽  
Reproductive Function ◽  
Egg Yolk ◽  
Final Concentration ◽  
Freezing Process ◽  
Soy Lecithin ◽  
Group A ◽  
Short And Long Term

A challenge in freezing semen for short and long-term availability is avoiding damage to intact spermatozoa caused by the freezing process. Vitrification protocols provide better results through less manipulation of semen and shorter freezing time compared to slow freezing techniques. Our research was aimed at improving vitrification methods for canine semen. Semen quality was determined in 20 ejaculates after collection. Each ejaculate was divided into eight aliquots, each with a different extender. The control extender contained TRIS, citric acid, fructose, and antibiotics. Soy lecithin and sucrose were added to the control extender at different concentrations to make up the test extenders and final concentration of 50 × 106 spermatozoa/mL. From each group, a 33 µL (1.65 × 106 spermatozoa) suspension of spermatozoa was dropped directly into liquid nitrogen and devitrified at least one week later and evaluated as before. Soy lecithin at 1% and 0.25 M sucrose added to the base vitrification media effectively preserved all sperm qualities. Our results demonstrate the effectiveness of our methods. Vitrification media containing sucrose and soy lecithin cause a minimal decline in quality of canine semen after devitrification. Furthermore, extenders used in our research did not contain egg yolk, which was replaced by soy lecithin, thus allowing for ease of shipping to other countries with strict requirements.

Effect of low-density lipoproteins and trehalose on cryopreserved bovine semen quality

2020 ◽  
Author(s):  
Elizabeth Varela-Giraldo ◽  
Alexandra Úsuga-Suárez ◽  
Juan E Uque-Cortés ◽  
Jorge Gómez-Oquendo ◽  
Giovanni Restrepo-Betancur
Keyword(s):  
Semen Quality ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Bovine Semen

scholarly journals Manganese Provides Antioxidant Protection for Sperm Cryopreservation that May Offer New Consideration for Clinical Fertility

2009 ◽  
Vol 2 (3) ◽  
pp. 152-159 ◽  
Author(s):  
Ranjna S. Cheema ◽  
Amrit K. Bansal ◽  
Gurmail Singh Bilaspuri
Keyword(s):  
Protective Effect ◽  
Semen Quality ◽  
Maximum Level ◽  
Egg Yolk ◽  
Quality Parameters ◽  
Vitro Fertilization ◽  
Bull Semen ◽  
Protein Leakage ◽  
A Chain

Reactive oxygen species (ROS) are generated by sperm metabolism. While, ROS are required for maturation, capacitation and acrosome reaction, they also modify many peroxidable cellular compounds. There is production of ROS during cryopreservation and frozen spermatozoa are highly sensitive to lipid peroxidation (LPO). Antioxidants exert a protective effect on the plasma membrane of frozen bovine sperm preserving both metabolic activity and cellular viability. Manganese (Mn++) is proved to be a chain breaking antioxidant in biological system. Therefore, we examined the role of (Mn++) during cryopreservation of cattle bull semen. Semen was divided into four parts and cryopreserved in egg-yolk-citrate extender + glycerol (EYC-G), EYC-G + 100 µM of Mn++, EYC-G + 150 µM of Mn++and EYC-G + 200 µM of Mn++. After four hours of cooling and 24 hrs of freezing, the spermatozoa were examined for percentage motility, Hypo-osmotic swelling (HOS), LPO and protein leakage. Addition of manganese to the semen during cryopreservation showed a protective effect and accounted for an increase in semen quality parameters [percentage motility, HOS percent and decrease in malondialdehyde (MDA) production and protein leakage]. The effect of manganese on motility and HOS was non-significant (p < 0.05) in cooled spermatozoa but significant with 150 µM of Mn++in frozen-thawed spermatozoa. MDA production and protein leakage decreased to a significant and maximum level (p < 0.05) on addition of 200 µM of manganese. The addition of manganese to EYC-G dilutor will improve the quality/fertility of semen, which will result in improvement of in vitro fertilization and artificial insemination success rate.

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