scholarly journals Analysis of genetic diversity of Arabica coffee [Coffea arabica L.] in Solok Regency by SRAP molecular markers

2020 ◽  
Vol 497 ◽  
pp. 012018 ◽  
Author(s):  
R Yunita ◽  
M Oktavioni ◽  
I Chaniago ◽  
L Syukriani ◽  
M A Setiawan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Coffea Arabica ◽  
Arabica Coffee ◽  
Coffea Arabica L

scholarly journals Assessment of coffee (coffea arabica L.) genetic erosion and genetic resources management in Ethiopia

2020 ◽  
Vol 7 (3) ◽  
pp. 223-229
Author(s):  
Afework Legesse
Keyword(s):  
Climate Change ◽  
Genetic Diversity ◽  
Coffea Arabica ◽  
Genetic Erosion ◽  
Evolutionary Potential ◽  
Arabica Coffee ◽  
Resources Management ◽  
Genetic Resources Management ◽  
Coffee Yield ◽  
Coffea Arabica L

Coffee is an important source of annual income and employment contributing significantly to the economies of many developing countries. Ethiopia is the center of origin and diversity of Coffea arabica L., there is immense genetic variability that offers great potential for improvement of the crop. The objective of this paper is to assess the status of Coffee genetic diversity, identify major factors that cause coffee genetic erosion and status of coffee genetics resources management in Ethiopia.  The presences of high genetic diversity in wild Coffea arabica in Ethiopia were reported by different authors. However, the genetic diversity of coffea arabica L. are being lost rapidly due to several factors such as human population pressures leading to conversion of land to agriculture, deforestation and land degradation; low coffee prices leading to abandoning of coffee trees in forests and gardens and shifting cultivation to other more remunerative crops; and climate change. Additionally, narrow genetic basis of commercially used Arabica coffee cultivars and increased incidence of pests and diseases associated with climate change is leading to significant crop losses, threatening livelihoods in many coffee growing countries. Therefore, Conserving the wild Arabica coffee gene pool and its evolutionary potential present in Ethiopia is critically important for maintaining coffee yield, disease resistance, drought tolerant, quality and other important traits in future breeding program

scholarly journals Identifikasi Molekular Kekerabatan Genetik Kopi Wamena Berbasis Marka Random Amplified of Polymorphic DNA (RAPD)

2021 ◽  
Vol 13 (1) ◽  
pp. 8-18
Author(s):  
I Made Budi ◽  
Arsyam Mawardi
Keyword(s):  
Molecular Markers ◽  
Genetic Relationship ◽  
Coffea Arabica ◽  
Random Amplified Polymorphic Dna ◽  
Habitat Destruction ◽  
Sample Collection ◽  
Arabica Coffee ◽  
Forest Clearing ◽  
Genetic Fingerprint ◽  
Coffea Arabica L

Baliem Coffee or Wamena Arabica Coffee is classified as a specialty coffee that has a distinctive aroma and taste as one of the world's best coffees. Wamena coffee has a high commercial value, there are many advantages of Wamena coffee. Growing on a variety of soil types, elevation, coffee variety, and durable. The quality of Wamena coffee must be maintained by the local government. However, this elite coffee germplasm is threatened with extinction and is in a dangerous situation due to habitat destruction due to forest clearing, land use change and the potential for competition with various disease-resistant varieties. This study aims to identify molecularly the genetic relationship of Wamena Arabica coffee in Jayawijaya district with the Random Amplified Polymorphic DNA (RAPD) markers as molecular markers. The stages in this study were sample collection, morphological observations, genomic DNA extracted using the CTAB method, gene amplification process using PCR-RAPD molecular markers with ten primers, measurement of DNA concentration using a spectrophotometer, and data analysis using UPGMA NTSYS version 2.1. The electroferogram results showed that pRAPD1, pRAPD2 and pRAPD5 primers produced a band pattern with high polymorphism as well as indicating the genetic diversity of the Coffea arabica L. arabica coffee samples tested. Based on the dendogram construction analysis, the arabica coffee sample Coffea arabica L. which was tested on 5 coffee genotypes, obtained 2 coffee groups, namely Group 1 consisting of one variety, namely sample (V) Arabica Typica from the Assolokobal area. Group 2 with four varieties, namely samples (I) Arabica Catimor, (II) USDA, (III) Arabica PM 88, and (IV) Arabica Linies 795, from Wollo. Phylogenetic tree construction resulted in a similarity coefficient of 83%, indicating the high relationship between Arabica coffee populations. The results of this study have obtained a genetic fingerprint profile of wamena specialty arabica coffee, namely its genetic relationship. The superiority of the genetic composition of Wamena coffee in Jayawijaya district in the future has the potential to be derived through much more advanced molecular technology.

Diversity in coffee assessed with SSR markers: structure of the genus Coffea and perspectives for breeding

Genome ◽  
2008 ◽  
Vol 51 (1) ◽  
pp. 50-63 ◽  
Author(s):  
Philippe Cubry ◽  
Pascal Musoli ◽  
Hyacinte Legnaté ◽  
David Pot ◽  
Fabien de Bellis ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Microsatellite Markers ◽  
Wild Species ◽  
Comparative Mapping ◽  
Coffea Arabica ◽  
Bac Libraries ◽  
Cultivated Species ◽  
Coffee Species ◽  
Coffea Arabica L

The present study shows transferability of microsatellite markers developed in the two cultivated coffee species ( Coffea arabica L. and C. canephora Pierre ex Froehn.) to 15 species representing the previously identified main groups of the genus Coffea. Evaluation of the genetic diversity and available resources within Coffea and development of molecular markers transferable across species are important steps for breeding of the two cultivated species. We worked on 15 species with 60 microsatellite markers developed using different strategies (SSR-enriched libraries, BAC libraries, gene sequences). We focused our analysis on 4 species used for commercial or breeding purposes. Our results establish the high transferability of microsatellite markers within Coffea. We show the large amount of diversity available within wild species for breeding applications. Finally we discuss the consequences for future comparative mapping studies and breeding of the two cultivated species.

scholarly journals Unveiling a unique genetic diversity of cultivated Coffea arabica L. in its main domestication center: Yemen

2021 ◽  
Author(s):  
C. Montagnon ◽  
A. Mahyoub ◽  
W. Solano ◽  
F. Sheibani
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Coffea Arabica ◽  
Genetic Improvement ◽  
The World ◽  
Genetic Clusters ◽  
Domestication Process ◽  
Almost All ◽  
Coffea Arabica L ◽  
Cup Quality

AbstractWhilst it is established that almost all cultivated coffee (Coffea arabica L.) varieties originated in Yemen after some coffee seeds were introduced into Yemen from neighboring Ethiopia, the actual coffee genetic diversity in Yemen and its significance to the coffee world had never been explored. We observed five genetic clusters. The first cluster, which we named the Ethiopian-Only (EO) cluster, was made up exclusively of the Ethiopian accessions. This cluster was clearly separated from the Yemen and cultivated varieties clusters, hence confirming the genetic distance between wild Ethiopian accessions and coffee cultivated varieties around the world. The second cluster, which we named the SL-17 cluster, was a small cluster of cultivated worldwide varieties and included no Yemen samples. Two other clusters were made up of worldwide varieties and Yemen samples. We named these the Yemen Typica-Bourbon cluster and the Yemen SL-34 cluster. Finally, we observed one cluster that was unique to Yemen and was not related to any known cultivated varieties and not even to any known Ethiopian accession: we name this cluster the New-Yemen cluster. We discuss the consequences of these findings and their potential to pave the way for further comprehensive genetic improvement projects for the identification of major resilience/adaptation and cup quality genes that have been shaped through the domestication process of C. arabica.

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

scholarly journals Organoleptic, Sensory and Biochemical Traits of Arabica Coffee and their Arabusta Hybrids

2021 ◽  
Author(s):  
Kahiu Ngugi ◽  
Jane Jerono Cheserek ◽  
Chrispine Ogutu Omondi
Keyword(s):  
Experimental Data ◽  
Genetic Diversity ◽  
Genetic Variation ◽  
Coffea Arabica ◽  
Small Scale ◽  
Arabica Coffee ◽  
Biochemical Compounds ◽  
Small Scale Farmers ◽  
Organoleptic Traits ◽  
Cup Quality

Coffee as a cash crop, reduces food insecurity by providing regular incomes and is a major foreign exchange earner in more than fifty tropical countries where it is grown either as Arabica (Coffea arabica) or Robust (Coffea canepora). In Kenya which grow some Robusta but mostly Arabica coffee, the production has been declining, mainly because world coffee prices have plummeted to about 5 USD for a 650Kg of un-hulled beans per acre. The only way world prices are likely to increase and benefit the small-scale farmers, is by improving the cup quality and enabling these countries to sell their coffee in specialty markets. This review, underscores the importance of analyzing and estimating organoleptic, sensory and biochemical compounds diversity in Arabica coffee, since these are the factors that determine cup quality. In an attempt to do so, the chapter presents experimental data that analyzed various sensory and organoleptic traits of Arabica coffee and their Arabusta hybrids that proves that tremendous genetic diversity exists in coffee genotypes grown in Kenya and it is possible to utilize this genetic variation to improve cup quality.

Teratogenic Effects of Arabica Coffee (Coffea arabica L) on Rats During the Organogenesis Period

2021 ◽  
Vol 3 (2) ◽  
pp. 25-31
Author(s):  
Reza Destri Anggi ◽  
Yuandani ◽  
Aminah Dalimunthe
Keyword(s):  
Weight Loss ◽  
Body Length ◽  
Coffea Arabica ◽  
Teratogenic Effect ◽  
Teratogenic Effects ◽  
Arabica Coffee ◽  
Coffea Arabica L

The purpose of the research was to evaluate the teratogenic effect caused by theprovision of Arabica coffee solution with variations of 0.36 ml, 0.72 ml and 1.08 ml withpositive control of caffeine at a dose of 300 mg / kg bw to rats during organogenesis. Themeasurement parameters in this study are the appearance of reproduction, externalmalformations and scalal malformations. The results in this study found that theadministration of Arabica coffee solution with a volume of 1.08 ml and caffeine 300 mg /kg bb caused an abnormality in the reproductive appearance of weight loss and body length,whereas for extrenal malformation and scalal malforation no abnormalities were found ineach administration of the solution Arabica coffee and caffeine.  

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