Evaluation of total phenolic, flavonoid contents, antioxidant and cytotoxicity activities of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit

In this study, the optimal condition for the extraction of antioxidants from the fruit Buah Mahkota Dewa (Phaleria macrocarpa) was determined by using Response Surface Methodology (RSM). The optimization was applied using central composite design (CCD) to investigate the three independent variables, namely extraction temperature (oC), extraction time (minutes) and extraction solvent to-feed ratio (%v/v) on the responses of free radical scavenging activity (DPPH), ferric ion reducing power assay (FRAP), total phenolic content (TPC) and total flavonoid content (TFC).The optimal conditions for the antioxidants extraction were found to be extraction temperature (64oC), extraction time (66 minutes) and solvent to-feed ratio (75 %v/v) with the highest percentage yield of DPPH, FRAP, TPC and TFC were 86.85%, 7.47%, 292.86 mg/g and 3.22 mg/g respectively. Moreover, the data were subjected to response surface methodology (RSM) and the results showed that the polynomial equations for all models were significant, did not show lack of fit, and presented adjusted determination coefficients (R2) above 99%, proving the yield of phenolic, flavonoid and antioxidants activities obtained experimentally were close to the predicted values and the suitability of the model employed in RSM to optimize the extraction conditions. Hence, in this study, the fruit from P.macrocarpa could be considered to have the strong antioxidant ability and can be used in various cosmeceutical or medicinal applications.

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In vitro evaluation of the total phenolic and flavonoid contents and the antimicrobial and cytotoxicity activities of crude fruit extracts with different polarities from Ficus sycomorus

Pacific Science Review A Natural Science and Engineering ◽

10.1016/j.psra.2016.02.002 ◽

2015 ◽

Vol 17 (3) ◽

pp. 103-108 ◽

Cited By ~ 4

Author(s):

Sheikha Khamis Al-matani ◽

Ruqaiya Nasser Said Al-Wahaibi ◽

Mohammed Amzad Hossain

Keyword(s):

Total Phenolic ◽

In Vitro Evaluation ◽

Fruit Extracts ◽

Ficus Sycomorus ◽

Cytotoxicity Activities ◽

Phenolic And Flavonoid

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Effect of extraction temperature and time on the extraction of phenolic compounds and antioxidant capacity of Phaleria macrocarpa fruit

Asia Proceedings of Social Sciences ◽

10.31580/apss.v2i1.271 ◽

2018 ◽

Vol 2 (1) ◽

pp. 14-18

Author(s):

Peng Yee Lim ◽

Sook Fun Pang ◽

Mashitah Mohd Yusoff ◽

Jolius Gimbun

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Pearson Correlation ◽

Total Phenolic ◽

Extraction Temperature ◽

Pearson Correlation Coefficient ◽

Flavonoid Content ◽

Phaleria Macrocarpa ◽

Optimum Extraction

Phaleria macrocarpa (ver. name: â€˜mahkota dewaâ€™) is a plant which has many medically useful antioxidant activities (Anggraini & Lewandowsky, 2015). The polyphenols responsible for this antioxidant activity has to be extracted before it can be routinely used (Shwter et al., 2016). This study investigates the extraction of polyphenols from P. macrocarpa fruits and its antioxidant activity (DPPH-RSA) under influence of extraction time and temperature. By employing maceration technique, the P. macrocarpa fruits extract showed the maximum total phenolic content (TPC), total flavonoid content (TFC) and DPPH-RSA with value of 69.5 mg QE/g DW, 183.2 mg GA/g DW and 171. 8 mg BHA/g DW, respectively at optimum extraction conditions of 60 min and 80 ÂºC (Fig. 1). Excellent and positive Pearson correlation coefficient with R2 > 0.91 between the TPC, TFC and antioxidant activities was observed.

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The influence of Gum Arabic on the physicochemical and antimicrobial activity of the microencapsulated Mahkota Dewa (Phaleria macrocarpa) leaves

Food Research ◽

10.26656/fr.2017.5(3).580 ◽

2021 ◽

Vol 5 (3) ◽

pp. 203-213

Author(s):

Z. Mohd Zin ◽

N.H. Razman ◽

Hasmadi M. ◽

M.N. Abd Manap ◽

M.K. Zainol

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Gum Arabic ◽

Total Phenolic ◽

Flavonoid Content ◽

Spherical Structure ◽

Ultrasonic Spray ◽

Phaleria Macrocarpa ◽

Coating Matrix ◽

Phenolic And Flavonoid

Mahkota Dewa (Phaleria macrocarpa) also known as God's Crown has been historically used as an indispensable alternative herbal medicine. Microencapsulation is a process whereby tiny particles or droplets are engulfed or enclosed in a coating matrix to produce small capsules. Generally, without microencapsulation, powders are fragile materials that could easily interfere with other components that are difficult to dissolve in water, lose their beneficial properties and decrease shelf life. It is hoped that the microencapsulation would increase the consistency of the powder during storage and maintain its beneficial properties. The goal of this research is to investigate the physicochemical and antimicrobial activity of Mahkota Dewa leaves encapsulated in different concentrations of gum Arabic (GA) and to determine the form of antioxidant and their role and properties. Mahkota Dewa leaves powders were microencapsulated in 0%, 2%, 4%, 6%, 8% and 10% gum Arabic using an ultrasonic spray dryer at 90οC. The microencapsulated Mahkota Dewa leaves (MMDL) samples were subjected to physicochemical and antimicrobial activity. The results showed that the 6% GA MMDL exhibited the highest yield (3.91%) while 0% GA was the lowest yield (1.64%). The highest total phenolic and flavonoid content was exhibited by 2% GA. The highest DPPH inhibition was depicted in 0% GA which indicates the highest antioxidant activity (54.9±0.01%) and is significantly (p<0.05) different from other samples. The highest inhibition was exhibited in 0% GA in the TBA method and FTC analysis. The encapsulated powders were identified to have weak antimicrobial activity against Bacillus cereus, Escherichia coli, Staphylococcus aureus, Salmonella and Listeria monocytogenes. The powders produced have an irregularly spherical structure and smooth surface with some dented spots on the surface. The different concentration of gum Arabic resulted in different antioxidant activity, flavonoid content and antimicrobial activity of MMDL.

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