Chemical Profiling of Pueraria lobata and Pueraria thomsonii using Total Phenolic, Flavonoid, Carbohydrate and Antioxidant Assays

Planta Medica ◽  
2012 ◽  
Vol 78 (05) ◽  
Author(s):  
KH Wong ◽  
GQ Li ◽  
KM Li ◽  
V Razmovski-Naumovski ◽  
K Chan
Keyword(s):  
Total Phenolic ◽  
Pueraria Lobata ◽  
Antioxidant Assays ◽  
Chemical Profiling

scholarly journals Contribution to the knowledge of the chemical composition, biological activities and activity concentration of 40K, 137Cs, 226Ra and 232Th of the lichen Evernia prunastri

2018 ◽  
Vol 83 (11) ◽  
pp. 1209-1221
Author(s):  
Gordana Stojanovic ◽  
Ivana Zlatanovic ◽  
Natasa Lazarevic ◽  
Violeta Mitic ◽  
Aleksandra Djordjevic ◽  
...  
Keyword(s):  
Activity Concentration ◽  
Cholinesterase Activity ◽  
Biological Activities ◽  
Human Lymphocytes ◽  
Total Phenolic ◽  
Gram Negative Bacteria ◽  
Total Phenolic Compounds ◽  
Evernia Prunastri ◽  
Chemical Profiling ◽  
Activity Concentrations

This study reports the effect of an acetone extract of Evernia prunastri on the micronucleus distribution of human lymphocytes and effect on cholinesterase activity. Furthermore, the antioxidant activity (estimated via DPPH, ABTS, TRP and CUPRAC assays), as well as total phenolic compounds (TPC) and antibacterial activity (against two Gram-positive and three Gram-negative bacteria) were determined. Chemical profiling of four E. prunastri extracts (acetone, diethyl ether, ethyl acetate and dichloromethane) was realized by GC?MS and HPLC analysis. In addition, the activity concentrations of 40K, 137Cs, 226Ra and 232Th were established.

scholarly journals Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae)

2020 ◽  
Vol 11 ◽  
Author(s):  
María José Larrazábal-Fuentes ◽  
Carlos Fernández-Galleguillos ◽  
Jenifer Palma-Ramírez ◽  
Javier Romero-Parra ◽  
Kevin Sepúlveda ◽  
...  
Keyword(s):  
Native Species ◽  
Total Flavonoid ◽  
Mass Spectrometry Analysis ◽  
Cholinesterase Inhibition ◽  
Total Phenolic ◽  
Protective Effects ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Spectrometry Analysis ◽  
Chemical Profiling

Artemisia copa Phil. (Asteraceae) (known as copa-copa) is a native species of Chile used as an infusion in traditional medicine by Atacameños people in the Altiplano, highlands of northern Chile. In this research, we have investigated for the first time the cholinesterase inhibition potential against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and the chemical profiling of the infusions prepared from the aerial parts of A. copa by high resolution spectrometry. In addition, total phenolic, total flavonoid content, antioxidant (DPPH, FRAP, and ORAC) and antiprozoal activity were tested. Artemisia copa showed good inhibitory activity against AChE and BChE (3.92 ± 0.08 µg/ml and 44.13 ± 0.10 µg/ml). The infusion displayed a total phenolics content of 155.6 ± 2.9 mg of gallic acid equivalents/g and total flavonoid content of 5.5 ± 0.2 mg quercetin equivalents/g. Additionally, trypanocidal activity against Trypanosoma cruzi was found (LD50 of 131.8 µg/ml). Forty-seven metabolites were detected in the infusion of A. copa including several phenolic acids and flavonoids which were rapidly identified using ultrahigh performance liquid chromatography orbitrap mass spectrometry analysis (UHPLC-Orbitrap-MS) for chemical profiling. The major compounds identified in the infusions were studied by molecular docking against AChE and BChE. The UHPLC-MS fingerprints generated can be also used for the authentication of these endemic species. These findings reveal that A. copa infusions can be used as beverages with protective effects.

scholarly journals Chemical and Biological Investigation of Apiculture Products from Stingless Bees Heterotrigona itama

2020 ◽  
Vol 11 (1) ◽  
pp. 7-19
Author(s):  
Khamsah Suryati Mohd ◽  
Nur Basyirah Md Zin
Keyword(s):  
Antioxidant Activity ◽  
Moisture Content ◽  
Stingless Bee ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Chemical Compositions ◽  
Nutritional Content ◽  
Chemical Profiling ◽  
Ic50 Value ◽  
Dpph Method

Heterotrigona itama is one of common stingless bee species in Malaysia. Similar to sting bee, stingless bee also produce honey, propolis and beebread in their hive. Propolis and beebread of Malaysian stingless bee were less explored compared to honey. This work examine nutritional content, antioxidant activity and chemical profiling of honey, propolis and beebread produced by stingless bee species Heterotrigona itama. Nutritional contents such as moisture content, ash, protein, fat and carbohydrate were measured. Antioxidant activity was determined using DPPH method; Folin-Ciocalteau method for total phenolic content (TPC) and Aluminium Chloride method for total flavonoid content (TFC). Chemical profiling was carried out by using thin layer chromatography (TLC) in solvent system of toluene: ethyl acetate: formic acid with ratio 8 : 2 : 0.1 v/v/v. The results revealed that honey possessed the highest moisture content and total carbohydrate with values of 30.67%±1.30 and 65.88%±1.70, respectively. Meanwhile, propolis contained the highest total fat (49.2% ± 5.31) which due to its compositions of wax and resin. Total protein and ash contents were abundant in beebread with values of 30.43%±0.40 and 3.28%±0.13, respectively. The highest total phenolic (70.64%±01.81 µg/mL) and flavonoid contents (31.75%±0.05 µg/mL) were found in propolis. Propolis also possessed the highest antioxidant activity with the lowest IC50 value of 5 µg/mL as comparable to positive control, Trolox (IC50 value: 3µg/mL). These results revealed that propolis is a good source of antioxidant as compared to honey and beebread. TLC analysis revealed that propolis also contained higher chemical compositions as compared to honey and beebread. This finding shows that stingless bee products have potential in therapeutic as strong natural antioxidant (propolis) and high in their nutritional content (honey and beebread).

scholarly journals Antioxidant and Antimicrobial Activity in Flower and Stem of Banana (Musa acuminata ‘Berangan’ and Musa X paradisiaca ‘Nangka’)

2020 ◽  
Vol 2 (1) ◽  
pp. 69
Keyword(s):  
Antimicrobial Activity ◽  
Musa Acuminata ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Steady Increase ◽  
Banana Plant ◽  
Antioxidant Assays ◽  
Musa Spp ◽  
Antioxidant And Antimicrobial Activity ◽  
Banana Flower

Banana plant (Musa spp.) is exploited in many researches as a potential source of therapeutic options to treat diseases. The aim of this study is to evaluate the antioxidant and antimicrobial activity of the banana flower and stem. Two selected Musa spp. banana plants were dried, powdered, and extracted using the Soxhlet method with solvents of increasing polarity, petroleum ether (PE), ethyl acetate (EA), and methanol (ME). The extracts were then subjected to antioxidant assays like DPPH, FRAP, Total Phenolic Count (TPC), and Total Flavonoid Count (TFC). The antimicrobial activity against selected microorganisms was investigated using the agar well diffusion method. The moisture content was found to be higher in banana stems compared to flowers. The banana flower extracts of increasing polarity shown a steady increase in all antioxidant assays, while the banana stem extracts were seen to vary across different antioxidant capacity assays. The highest in DPPH assay was Musa acuminata ‘Berangan’ flower (91.8%) ME extract, FRAP with Musa x Paradisiaca ‘Nangka’ stem (4393.9 mg BHT/g) PE extract, TPC (594.85 mg GAE/g) and TFC (391.01 mg QE/g) with Musa x Paradisiaca ‘Nangka’ stem EA extract. The extracts showed higher antimicrobial activity against S.aureus, followed by E.coli and E.faecalis. The EA extract of Musa x Paradisiaca ‘Nangka’ stem recorded the highest activity across the antioxidant, and antimicrobial assay carried out in this study. In conclusion, both the flower and the stem exhibited good antioxidant and antimicrobial capacity in solvent of increasing polarity.

scholarly journals Various antioxidant assays of agarwood extracts (Gyrinops versteegii) from West Lombok, West Nusa Tenggara, Indonesia

2019 ◽  
Vol 3 (01) ◽  
pp. 1-5 ◽  
Author(s):  
AMALIA INDAH PRIHANTINI ◽  
KANTI DEWI RIZQIANI
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dry Extract ◽  
Antioxidant Assays ◽  
Β Carotene

Abstract. Prihantini AI, Rizqiani K. 2019. Various antioxidant assays of agarwood extracts (Gyrinops versteegii) from West Lombok, West Nusa Tenggara, Indonesia. Asian J Agric 3: 1-5. However, the species have not been widely explored as a source of natural products in particular antioxidant agents, which protect cells from damage caused by free radicals. The present study was aimed to evaluate antioxidant activities of agarwood extracts from West Nusa Tenggara using various antioxidant assays. The antioxidant activity of leaf, fruit and fruit bark extracts were investigated based on DPPH radicals scavenging activity, reducing power, and β-carotene bleaching assays. The total phenolic content was also investigated. The result showed that leaf extract revealed the strongest antioxidant activity on all assays performed such as DPPH radicals scavenging activity (IC50 22.13±0.71 μg/mL); reducing power (251.85±0.03 mg QE/g dry extract); and β-carotene bleaching activity (IC50 24.23±2.60 μg/mL). The total phenolic content (TPC) in the leaf was higher (184.90±0.76 mg GAE/g dry extract) than fruit bark and bark extracts. The high content of phenolic compounds in G. versteegii leaves indicated that these compounds might contribute to the antioxidant activities. In conclusion, these findings showed that G. versteegii leaves are potential for development as an antioxidant source.

scholarly journals Hystrix brachyura Bezoar Characterization, Antioxidant Activity Screening, and Anticancer Activity on Melanoma Cells (A375): A Preliminary Study

Antioxidants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 39 ◽  
Author(s):  
Al’aina Yuhainis Firus Khan ◽  
Faizah Abdullah Asuhaimi ◽  
Tara K. Jalal ◽  
Fatimah Opeyemi Roheem ◽  
Hatim Abdullah Natto ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Melanoma Cells ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Antioxidant Assays ◽  
Β Carotene

Porcupine bezoars (PBs) are masses of undigested calcareous concretions formed within the gastrointestinal tract. There are undocumented claims that PBs have antioxidant activity and can treat cancers. However, limited scientific study has been carried out to verify these traditional claims. Hence, this study was conducted to characterize the chemical profile and validate the antioxidant and anticancer activity against melanoma cells (A375). PB extract was initially subjected to Fourier-transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GCMS), total phenolic content (TPC), and total flavonoid content (TFC) analyses. The bioautography of antioxidant assays, namely 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazy (DPPH), and β-carotene was performed. An in vitro A375 cell viability assay, apoptosis assay, cell cycle arrest assay, and gene expression assay were carried out as well. The experimental finding revealed 5,10-diethoxy-2,3,7,8-tetrahydro-1H,6H-dipyrrolo[1,2-a:1’,2’-d]pyrazine, ursodeoxycholic acid, and cholest-5-en-3-ol (3 beta)-, carbonochloridate are major compounds detected in PB extract. PB extract has low phenolic content, viz. 698.7 ± 0.93 (µg GAE/5 mg dry weight). The bioautography antioxidant assays revealed a potent antioxidant effect (ABTS > DPPH > β-carotene), with free radical scavenging activity. Furthermore, PB extract exhibited dose- and time-dependent inhibition of cancer activity on A375 cells due to the exhibition of apoptosis via an intrinsic pathway.

scholarly journals Inclusive Scenario of Natural Products Obtained From Agar Plant (Aquilaria SP.) In Bangladesh- A Review

2021 ◽  
Vol 29 (2) ◽  
pp. 151-173
Author(s):  
MN Hoque ◽  
MF Mondal ◽  
GHM Sagor ◽  
MM Hasan ◽  
A Hannan
Keyword(s):  
Chemical Constituents ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Chemical Profiling ◽  
Phenolic Contents ◽  
Volatile Aromatic Compounds ◽  
Positive Results ◽  
Medicines Quality

Agarwood is the resinous heartwood obtained from the injured parts of trees under Thymelaeaceae family particularly from Aquilaria species. Agarwood is considered as the most prized non-timber forest product (NTFP) used in attars as well as medicines. Quality of agarwood is the determiner for defining its commercial value. Different countries use different grading system to explain the quality of agarwood. In Bangladesh, more than 45 compounds have been identified so far, mostly sesquiterpenoids, alkanes, fatty acid and other volatile aromatic compounds that are responsible for its fragrance and high prices. Ether extract, total phenolic contents, flavonoid, antioxidant and microbiological tests have shown tremendous positive results. Chemical profiling of agar products is the best identified using GC-MS technique by the various authors. Present review discusses the full scenario of Aquilaria production, inoculation, extraction methods, determination of agarwood quality and chemical constituents of agar oils and possibilities and barriers of this industry in Bangladesh. J. Bio-Sci. 29(2): 151-173, 2021 (December)

scholarly journals COMPARISON OF FIVE ANTIOXIDANT ASSAYS FOR ESTIMATING ANTIOXIDANT CAPACITY FROM THREE SOLANUM SP. EXTRACTS

2016 ◽  
pp. 123 ◽  
Author(s):  
Irda Fidrianny ◽  
Atina Rizkiya Choirunnisa ◽  
Komar Ruslan
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Linear Correlation ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Major Contributor ◽  
Fruit Extract ◽  
Antioxidant Assays ◽  
Fruit Extracts ◽  
Black Nightshade

<p>ABSTRACT<br />Objectives: The aims of this research were to determine antioxidant capacity of various extracts from black nightshade, turkey berry, and round<br />green eggplant using five antioxidant assays which were ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC),<br />H<br />2<br />O<br /> scavenging, phosphomolybdenum assay, and beta-carotene bleaching (BCB), correlation of total phenolic, flavonoid, and carotenoid content with<br />their inhibitory concentration 50% (IC<br />2<br />50<br />) and exhibitory concentration 50% (EC<br />) of five antioxidant assay and correlation between five antioxidant<br />assays.<br />50<br />Methods: Extraction was conducted by reflux using gradient polarity solvents. The extracts were evaporated using rotary evaporator. The antioxidant<br />capacity study, determination of phenolic, flavonoid, and carotenoid content were performed by ultraviolet-visible spectophotometry, while its<br />correlation with IC<br />50<br /> and EC<br />50<br /> of five methods were analyzed by Pearson’s method.<br />Results: Ethanolic and ethyl acetate fruit extracts of turkey berry denoted the highest antioxidant capacity using FRAP (EC<br />41.32 µg/ml),<br />H<br />2<br />O<br />2<br /> scavenging assay (IC<br />50: <br />1.01 µg/ml), and CUPRAC (EC<br />117.56 µg/ml). While ethyl acetate fruit extract of round green eggplant gave the<br />highest phosphomolybdenum capacity (EC<br />50<br />50: <br />: 375.47 µg/ml), and ethyl acetate fruit extract of black nightshade showed the highest BCB capacity<br />(EC<br />50: <br />158.66 µg/ml). Phenolic content of all fruit extracts had a tendency to correlate with FRAP and H<br />2<br />O<br /> scavenging antioxidant capacity, meanwhile<br />flavonoid and carotenoid content had a tendency to correlate with CUPRAC, phosphomolybdenum, and BCB antioxidant capacity.<br />2<br />Conclusions: Phenolic compounds were a major contributor in antioxidant capacity of black nightshade, turkey berry, and round green eggplant<br />extracts using FRAP and H<br />2<br />O<br /> scavenging, meanwhile flavonoid and carotenoid compounds were a major contributor in antioxidant capacity using<br />CUPRAC, phosphomolybdenum and BCB assays. FRAP assay had linear correlation with H<br />2<br />2<br />O<br /> scavenging, meanwhile CUPRAC had linear correlation<br />with phosphomolybdenum and BCB.<br />Keywords: Antioxidant, Antioxidant assays, Solanum fruits.<br />2</p><p>50:</p>

scholarly journals Chemical Profiling of Polyfloral Belgian Honey: Ellagic Acid and Pinocembrin as Antioxidants and Chemical Markers

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Izabela Jasicka-Misiak ◽  
Steven Gruyaert ◽  
Anna Poliwoda ◽  
Paweł Kafarski
Keyword(s):  
Antioxidant Activity ◽  
Ellagic Acid ◽  
Total Phenolic ◽  
Chemical Markers ◽  
Chemical Marker ◽  
Standard Analysis ◽  
Phenolic Fraction ◽  
Phenolic Profile ◽  
Chemical Profiling ◽  
Phenolic And Flavonoid

Chemical profiling of northern Belgian polyfloral honeys was performed to analyse their phenolic compound content (flavonoids and phenolic acids). First, samples were subjected to a standard analysis of their physicochemical properties, and then, the phenolic fraction was isolated and analysed using a HPLC/PAD method. All of the tested honeys showed a common and specific phenolic profile that could be the basis for the differentiation of polyfloral honeys of the Antwerp region from other polyfloral honeys. Chromatographic data indicated a high content of ellagic acid (9.13–13.66 mg/100 g honey), as well as the flavonoid pinocembrin (1.60–1.85 mg/100 g honey) in these honeys. Ellagic acid, a compound with well-defined prohealth activities, might be used as a chemical marker for these honeys. With respect to total phenolic and flavonoid contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were determined spectrophotometrically. The honey exhibited a moderate antioxidant activity, typical for light honeys.

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