scholarly journals Development of Phaleria macrocarpa (Scheff.) Boerl Fruits Using Response Surface Methodology On Phenolic, Flavonoid and Antioxidants Properties

2018 ◽  
Author(s):  
Khurul Ain Mohamed Mahzir ◽  
Siti Salwa Abdul Gani ◽  
Nor Fadzillah Mokhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Time ◽  
Feed Ratio ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Phaleria Macrocarpa

In this study, the optimal condition for the extraction of antioxidants from the fruit Buah Mahkota Dewa (Phaleria macrocarpa) was determined by using Response Surface Methodology (RSM). The optimization was applied using central composite design (CCD) to investigate the three independent variables, namely extraction temperature (oC), extraction time (minutes) and extraction solvent to-feed ratio (%v/v) on the responses of free radical scavenging activity (DPPH), ferric ion reducing power assay (FRAP), total phenolic content (TPC) and total flavonoid content (TFC).The optimal conditions for the antioxidants extraction were found to be extraction temperature (64oC), extraction time (66 minutes) and solvent to-feed ratio (75 %v/v) with the highest percentage yield of DPPH, FRAP, TPC and TFC were 86.85%, 7.47%, 292.86 mg/g and 3.22 mg/g respectively. Moreover, the data were subjected to response surface methodology (RSM) and the results showed that the polynomial equations for all models were significant, did not show lack of fit, and presented adjusted determination coefficients (R2) above 99%, proving the yield of phenolic, flavonoid and antioxidants activities obtained experimentally were close to the predicted values and the suitability of the model employed in RSM to optimize the extraction conditions. Hence, in this study, the fruit from P.macrocarpa could be considered to have the strong antioxidant ability and can be used in various cosmeceutical or medicinal applications.

EXTRACTION OPTIMIZATION OF THREE INDEPENDENT VARIABLES OF ANTIOXIDANTS FROM BASIL LEAVES USING RESPONSE SURFACE METHODOLOGY

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (04) ◽  
pp. 20-28 ◽  
Author(s):  
Sulaiman M. Alnasser ◽  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Optimum Conditions ◽  
Antioxidant Power ◽  
Independent Variables

The current study reports the antioxidant activity of Ocimum basilicum. Central composite design (CCD) was used to study the effect of variables on the extraction using magnetic stirrer (MST). Three independent variables including temperature, extraction speed, and extraction time were studied that optimize particular responses of total phenolic content, ferric reducing antioxidant power (FRAP), and DPPH free radical scavenging activity. The effect of the extraction temperature on extraction process was investigated in the range from 26.3oC to 93.6oC, extraction time, from 1 to 30 h, and extraction speed, in the range from 263 to 936 rpm. The extraction yield was significantly (P < 0.05) affected by all the variables. Temperature and extraction speed were found to have a marked effect while the extraction time was found to have possible minor effects. Graphical optimization determined the optimum conditions for the extraction. The optimum condition predicted an extraction yield of 20.49g/100g at 75.33oC for five hours at 73 rpm. Optimum conditions were determined to obtain highest extraction yield. Results showed that water/seed ratio was the most significant parameter, followed by temperature and time. All quantitative modeling and response surface methodology recommended that extraction temperature and time were the most effective parameters of MST process. However, extraction time was found out to be an insignificant factor in MST extraction of antioxidant and total phenolic compounds of O. basilicum.

Characterization and Antioxidant Activity of Flash-Assisted Extracted Dihydroquercetin from Wood Sawdust of Larix gmelinii Using a Response Surface Methodology

2016 ◽  
Vol 12 (6) ◽  
pp. 587-597 ◽  
Author(s):  
Jianxia Li ◽  
Qiaohui Zhang ◽  
Jie Cui ◽  
Yaping Ning ◽  
Fengjun Wang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Larix Gmelinii ◽  
Wood Sawdust ◽  
Assisted Extraction

Abstract We investigate a flash-assisted extraction of dihydroquercetin (DHQ) from wood sawdust of Larix gmelinii by response surface methodology. Box–Behnken design optimized the extraction conditions, and the highest DHQ yield (6.15 %) was obtained under optimal extraction conditions: FAE time (3 min), extraction time (3 h), solid-to-liquid (g/mL) ratio (1:15), and ethanol concentration (75 %). After purification, the DHQ purity was 96 % as determined by HPLC and was further characterized by FTIR. Purified DHQ’s antioxidant activities were confirmed in vitro by determining DPPH and ABTS radical scavenging activities and reducing Fe3+ power. In the DPPH and ABTS radical scavenging activity assays, DHQ displayed prominent antioxidant activities with low IC50 values (11.568 and 12.475 μg/mL, respectively). In reducing Fe3+ power assay, high DHQ absorbance values showed that DHQ had higher reducing power than butylated hydroxyl toluene and had nearly the same consistent power as ascorbic acid at the same doses.

Optimization of Enzyme-Assisted Extraction of Carotenoids Antioxidants from Cordyceps militaris Using Response Surface Methodology

2017 ◽  
Vol 13 (5) ◽  
Author(s):  
Bai-Juan Wang ◽  
Qing-Song Yang ◽  
Ting Chen ◽  
Xiang-Dong Qin ◽  
Jun-Rong Ma ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cordyceps Militaris ◽  
Extraction Temperature ◽  
Scavenging Activity ◽  
Assisted Extraction ◽  
Development And Utilization ◽  
Enzyme Assisted Extraction

Abstract Response surface methodology (RSM) with a Box–Behnken design (BBD) was used to optimize the extraction conditions of carotenoid antioxidants from Cordyceps militaris. An enzyme-assisted extraction (EAE) method was developed and optimized to enhance 2,2-diphenyl-1-picrylhydrazil (DPPH) radical-scavenging activity of carotenoid antioxidants from C. militaris. The optimum conditions were as follows: a pH of 4.1, an extraction time of 40.4 min, an extraction temperature of 48.7 °C and 0.43 % enzyme (cellulose: pectinase, 1:1, w/w) concentration. Under these conditions, the DDPH radical-scavenging activity of C. militaris extracts was 81.62±2.26 %, indicating that the BBD is an efficient approach to develop mathematical models for predictingcarotenoids antioxidant extraction from C. militaris. The successful extraction of carotenoid antioxidants from C. militaris provides a basis for the development and utilization of C. militaris resources.

scholarly journals Optimization of Extraction of Total Phenolic and Total Flavonoid from Seeds of Alpinia blepharocalyx K. Schum. Using The Response Surface Methodology

2019 ◽  
Vol 57 (3B) ◽  
pp. 105
Author(s):  
Thanh Tan Nguyen ◽  
Huyen Thi Thanh Mai ◽  
Tuan Ngoc Nguyen ◽  
Quang Dinh Tran ◽  
Thang Dinh Tran
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Flavonoid Compounds ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Seeds of Alpinia blepharocalyx contain high amounts of phenolic and flavonoid compounds with potential antioxidant properties. In this study, the effects of the extraction method on the recovery of phenolic and flavonoid compounds from Alpinia blepharocalyx seeds were investigated. Response surface methodology (RSM) has been used to optimize the extraction conditions of total phenolic and total flavonoid from seeds of Alpinia blepharocalyx. A Box-Behnken design was used to investigate the effects of four independent variables, namely extraction temperature (°C), extraction time (min), (v/m) and ethanol concentration (%) on the responses: total phenolic content (TPC) and yield. The optimal conditions obtained from response RSM were 52.66 % v/v for the solvent composition, 62.34 (°C) for extraction temperature, 34.48/1 (ml/g) for solvent/material ratio and 125.42 (min) for extraction time. The experimental values of TPC, TFC and yield were 39.31±0.05 mgGAE/g, 12.75±0.07 mgCE/g and 6.97±0.05%, respectively.   

Analysis of Antioxidant Activity and Total Phenolic Content in Garcinia mangostana Using Response Surface Methodology

2015 ◽  
Vol 1113 ◽  
pp. 217-222
Author(s):  
Ummi Kalthum Ibrahim ◽  
Amira Sofea Mahamad Husin ◽  
Ruzitah Mohd Salleh
Keyword(s):  
Antioxidant Activity ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Garcinia Mangostana ◽  
Different Parts

This study investigates the antioxidant activity and total phenolic content on different parts of Garcinia mangostana which are pericarp, leaf, fruit, and seed. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to determine the antioxidant activity and the total phenolic content was determined by Folin-Ciocalteu method. Response surface method (RSM) was used to determine the effect of the two extraction variables: extraction time (30-120 minutes) and extraction temperature (30-50°C) on yield of total phenolic content and antioxidant activity of different parts of Garcinia mangostana. The significant factors on each experimental design response were identified from the analysis of variance (ANOVA). The best condition for total phenolic content of different parts of Garcinia mangostana was chosen based on the desirability obtained by using extraction temperature of 30°C and extraction time of 60.09 minutes, which resulted in 0.0274 mg GAE/g of total phenolic content in pericarp, 0.2501 mg GAE/g of total phenolic content in leaf, 0.0202 mg GAE/g of total phenolic content in fruit, and 0.0198 mg GAE/g of total phenolic content in seed of Garcinia mangostana. Under this conditions, it was found that the antioxidant activity of pericarp, leaf, fruit, and seed of Garcinia mangostana were 89.45%, 86.58%, 93.33%, and 78.80% of radical scavenging activity, respectively.

scholarly journals Process Optimization of Ultrasonic-Assisted Extraction of Arabinogalactan from Dihydroquercetin Extracted Residues by Response Surface Methodology and Evaluation of Its Antioxidant Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Zaizhi Liu ◽  
Chunhui Ma ◽  
Lei Yang ◽  
Yuangang Zu
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Before And After ◽  
Ultrasonic Assisted ◽  
Optimal Extraction Condition

Ultrasound was used for the extraction of larch arabinogalactan fromLarix gmelinii. The optimal conditions for ultrasound extraction were determined by response surface methodology. Specifically, the Box-Behnken design was employed to evaluate the effects of three independent variables: ultrasound time, temperature, and liquid-solid ratio. The highest arabinogalactan yield (11.18%) was obtained under the optimal extraction condition (extraction temperature 41.5°C, extraction time 24.3 min, and liquid-solid ratio 40 mL/g). In addition, the antioxidant activity of arabinogalactan that was extracted from dihydroquercetin extraction residues exhibited a moderate and concentration-dependent hydroxyl radical-scavenging capacity, ferric-reducing power, and reducing power. The wood material was characterized before and after processing by scanning electron microscopy and Fourier-transform infrared spectroscopy.

Biological activities of Saussurea lappa antioxidants recovered by solid-liquid, ultrasound and Ired-Irrad®

2020 ◽  
Vol 16 ◽  
Author(s):  
Hiba N. Rajha ◽  
Gisèle El Khoury ◽  
Nada El Darra ◽  
Karim Raafat ◽  
Espérance Debs ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Maximum Yield ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Medicinal Uses ◽  
Saussurea Lappa ◽  
Solid Liquid

Background: Saussurea lappa is a traditionally well-known plant appreciated for its biological activities and medicinal uses. Objective: In the present study, the recovery of antioxidants from Saussurea lappa was optimized using Response Surface Methodology (RSM). The efficiency of a newly-patented infrared (IR) technology, Ired-Irrad®, was compared to that of the emerging ultrasound method (US) and the conventional solid liquid water bath (WB) extraction. Methods: The effects of time (t) and temperature (T), mostly known to affect the extraction process, were tested on maximizing the total phenolic compounds concentration (TPC) and the radical scavenging activity (AA). Response surface methodology was used for the optimization process. Results: A multiple response optimization of both time (t) and temperature (T) was conducted, showing the best extraction conditions to be for WB: t= 43.86 min, T=33.79°C, for US: t= 65.47 min, T= 57.97°C and for IR: t= 42.5 min, T=34.19°C. The quantity of the optimally extracted polyphenols by WB, US and IR; as well as many of their bioactivities were compared. IR extraction gave the highest yield of TPC (15.3 mg GAE/g DM) followed by US (14.8 mg GAE/g DM) and lastly WB (13.9 mg GAE/g DM). The highest antioxidant and antiradical activities were also obtained by the IR treatment. The optimal IR extract inhibited the growth of Staphylococcus aureus and Escherichia coli up to 65 and 35%, respectively. Moreover, all Saussurea lappa extracts (WB, US and IR) inhibited up to 96% the production of Aflatoxin B1 (AFB1) by Aspergillus flavus. Conclusion: Our findings on the extraction of antioxidants from Saussurea lappa demonstrated that IR technology is an efficient novel method that can be used to extract the maximum yield of polyphenols, with the highest antioxidant, antiradical and antibacterial activities.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolics from Sideritis raeseri Using Response Surface Methodology

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3949
Author(s):  
Katarina Šavikin ◽  
Jelena Živković ◽  
Teodora Janković ◽  
Nada Ćujić-Nikolić ◽  
Gordana Zdunić ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In this study we define the optimal conditions for ultrasound-assisted extraction of bioactive polyphenols from S. raeseri aerial parts using response surface methodology. The influence of ethanol concentration (10–90%), extraction temperature (20–80 °C), extraction time (10–60 min), and solid-to-solvent ratio (1:10–1:50) on total phenolic content as well as on content of individual flavonoids, and hypolaetin and isoscutellarein derivatives was studied. For the experimental design, a central composite design was chosen. In the obtained extracts, the following ranges of targeted compounds were detected: total phenol from 19.32 to 47.23 mg GAE/g dw, HYP from 1.05 to 11.46 mg/g dw, ISC 1 from 0.68 to 10.68 mg/g dw, and ISC 2 from 0.74 to 15.56 mg/g dw. The optimal extraction conditions were set as: ethanol concentration of 65%, extraction time of 50 min, extraction temperature of 63 °C, and solid-to-solvent ratio of 1:40. Contents of TP, HYP, ISC 1, and ISC 2 in optimal extracts were 47.11 mg GAE/g dw, 11.73 mg/g dw, 9.54 mg/g dw, and 15.40 mg/g dw, respectively. Experimentally set values were in good agreement with those predicted by the response surface methodology model, indicating suitability of the used model, as well as the success of response surface methodology in optimizing the conditions of the extraction.

Optimization of Extraction of D-pinitol and Phenolics from Cultivated and Wild Types of Carob Pods Using Response Surface Methodology

2011 ◽  
Vol 7 (6) ◽  
Author(s):  
Irfan Turhan
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Dilution Rate ◽  
Response Surface ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Total Phenolic Compounds ◽  
Beneficial Effects ◽  
Carob Pod

The carob pod, an important fruit of the food industry in the production of concentrated syrup, is rich in potentially health-promoting phenolic compounds and sugars. Moreover, one of the important bioactive components of carob is D-pinitol, and it has some beneficial effects on human metabolism. The optimum conditions for extraction of D-pinitol and total phenolic compounds (TPC) from cultivated and wild types of carob pods were determined using response surface methodology (RSM). The Box-Behnken Design (BBD) was used to investigate the effects of three independent variables; extraction temperature (ºC), dilution rate (w/v), and extraction time (h) on the response, D-pinitol and the total phenolic compounds. The optimum extraction conditions obtained using the response optimizer were an extraction temperature of 80ºC, a dilution rate of 1:4, and an extraction time of 2h. Under the mentioned above conditions, the maximum D-pinitol and TPC concentrations were 9.67 g/L and 5916.55 mg/L in wild carob pod extract, respectively.

scholarly journals Accelerated solvent extraction of bioactive compounds from carrot: Optimization of response surface methodology

2018 ◽  
Vol 83 (11) ◽  
pp. 1223-1228 ◽  
Author(s):  
Vanja Seregelj ◽  
Vesna Tumbas-Saponjac ◽  
Anamarija Mandic ◽  
Gordana Cetkovic ◽  
Jasna Canadanovic-Brunet ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Solvent Extraction ◽  
Response Surface ◽  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Accelerated Solvent Extraction ◽  
Carotenoid Content ◽  
Extraction Temperature ◽  
Significant Difference

Carrot is considered to be rich in bioactive antioxidants, both lipophilic (carotenoids) and hydrophilic (phenolic compounds). In the present study, the conditions for accelerated solvent extraction (ASE) of bioactive compounds from carrots (Daucus carota L.) were optimized using response surface methodology (RSM). Box?Behnken design was employed for the experimental design to obtain the optimized combination of extraction temperature, time, and number of extraction cycles. Total carotenoid content (TCar), total polyphenol content (TPh), free radical scavenging activity (SA) and reducing power (RP) of the obtained extracts were used as responses for the optimization. Considering the four quality indicators, the ideal extraction conditions were found to be: 120 ?C, 60 min and three extraction cycles. Under these conditions, predicted values of 28.84 mg ?-carotene/100 g for TCar; 530.81 mg GAE/100 g for TPh; 2572.29 ?mol TE/100 g for SA and 1336.26 ?mol TE/100 g for RP were obtained with high desirability (0.975) and no significant difference (p < 0.05) with the experimental values.

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