scholarly journals Effect of dose and time of saline-tolerant Rhizobium sp. inoculation to productivity of black soybean (Glycine soja (l) merrit) on saline media

2021 ◽  
Vol 803 (1) ◽  
pp. 012024
Author(s):  
A.S.L. Mariati ◽  
E. Fuskhah ◽  
E. D. Purbajanti
Keyword(s):  
Glycine Soja ◽  
Black Soybean ◽  
Rhizobium Sp

scholarly journals Pengaruh pemberian ekstrak kedelai hitam (Glycine soja.) berbagai dosis terhadap kadar glukosa darah, kadar insulin, dan HOMA-IR

2017 ◽  
Vol 6 (1) ◽  
pp. 44-50
Author(s):  
Dewi M Kurniawati ◽  
Edi Dharmana ◽  
Banundari Rachmawati ◽  
Tjokorda Gde Dalem Pemayun
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Glycine Soja ◽  
Sprague Dawley ◽  
Black Soybean ◽  
Insulin Levels ◽  
Soybean Extract ◽  
Before And After ◽  
Post Test ◽  
Decrease Insulin Sensitivity

Background: Obese is risk factor for abnormal glucose homeostasis and causes oxidative stress that can decrease insulin sensitivity resulting in hyperglycemia and hyperinsulinemia. Black soy bean contains anthocyanins and isoflavones, it is expected to reduce oxidative stress. Objective: Determine the effect of various dosage of black soybean extract (Glycine soja.) on blood glucose, insulin levels, and HOMA - IR in obese Sprague Dawley (SD) rats.Methods: True experimental study with randomized controlled pre test-post test design in male SD rats. Twenty four samples divided into 4 groups: control (K), 500 (P1), 750 (P2), and 1000 mg/kgBW (P3). Rats were induced to become obese with High Fat Sucrose Diet (HFSD) for 4 weeks then black soybean extracts were given for 2 weeks..Results: There was no difference in blood glucose before and after treatment (p> 0.05). There were differences on insulin levels in 500 (p = 0.012) and 750mg/kgBW (p = 0.020) doseges. There were differences on HOMA-IR in 500 (p = 0.043) and 750mg/kgBW (p = 0.028) dosages. There was difference in insulin levels’s difference (p = 0.009) between groups.Conclusion: There are differences on insulin levels and HOMA – IR in 500 and 750 mg/kgBW dosages groups. However, there is no difference on blood glucose before and after black soybean extract treatment. The most decreased levels of blood glucose, insulin and HOMA- IR were in 750mg/kgBW dosage.

Purification of glysojanin, an antifungal protein, from the black soybean Glycine soja

2003 ◽  
Vol 81 (6) ◽  
pp. 387-394 ◽  
Author(s):  
Patrick H.K Ngai ◽  
T B Ng
Keyword(s):  
Liquid Chromatography ◽  
Ion Exchange ◽  
Glycine Soja ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Fast Protein Liquid Chromatography ◽  
Antifungal Protein ◽  
Black Soybean

A monomeric protein, with a molecular mass of 25 kDa and an N-terminal sequence resembling a segment of chitin synthase, was isolated from the seeds of the black soybean Glycine soja. The protein, designated glysojanin, demonstrated potent antifungal activity against the fungi Fusarium oxysporum and Mycosphaerella arachidicola. It inhibited HIV-1 reverse transcriptase with an IC50 of 47 µmol/L, [methyl-3H]thymidine incorporation by mouse spleen cells with an IC50 of 175 µmol/L, and translation in the rabbit reticulocyte lysate with an IC50 of 20 µmol/L. Glysojanin was purified using a procedure that involved ion-exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion-exchange chromatography by fast protein liquid chromatography on Mono S, and gel filtration by fast protein liquid chromatography on Superdex 75.Key words: antifungal protein, seeds, soybean, purification.

Rhizobium infection threads in root hairs of Glycine max (L.) Merr., Glycine soja Sieb. & Zucc, and Vigna unguiculata (L.) Walp.

1983 ◽  
Vol 29 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Steven G. Pueppke
Keyword(s):  
Glycine Max ◽  
Vigna Unguiculata ◽  
Glycine Soja ◽  
Primary Root ◽  
Wild Soybean ◽  
Root Hairs ◽  
Infected Root ◽  
Infection Threads ◽  
Basal Portion ◽  
Rhizobium Sp

Eight lines of soybean (Glycine max), four of wild soybean (Glycine soja), and one cowpea (Vigna unguiculata) cultivar were inoculated with 18 Rhizobium strains. After 4 days, root hairs were examined for infection threads. Threads were produced by all hosts but exclusively in nodulating combinations. Only Rhizobium sp. strains 3G4b9a and 3G4b19 were inconsistent; they nodulated soybean and G. soja in some experiments, but rarely formed infection threads. Soybean and G. soja were indistinguishable in their interactions with rhizobia, as were lele soybean lines (genetically lack soybean lectin), Hardee soybean (contains the noduation-influencing genes Rj2 and Rj3), and several other soybean cultivars. Threads formed in cowpea with all of the R. japonicum strains and most Rhizobium sp. but not with R. lupini. Infection of all three host species occurred in portions of the primary root containing immature or no root hairs at the time of inoculation; proximal tissues having elongated root hairs lacked infection threads. Infected root hairs were short and commonly shaped like question marks. Threads usually branched and sometimes intertwined prior to elongation into the basal portion of root hairs.

scholarly journals PERTUMBUHAN DAN PRODUKSI 2 VARIETAS KEDELAI HITAM (Glycine soja) TERHADAP PEMBERIAN BEBERAPA JENIS PUPUK ORGANIK (BOKASHI) DI LAHAN KERING

2020 ◽  
Vol 3 (2) ◽  
pp. 129-142
Author(s):  
Muhammad Rizal ◽  
St Subaedah ◽  
Aminah Muchdar
Keyword(s):  
Rice Straw ◽  
Agricultural Development ◽  
Glycine Soja ◽  
Organic Fertilizer ◽  
Farming System ◽  
Organic Fertilizers ◽  
Black Soybean ◽  
Significant Difference ◽  
Soybean Plants ◽  
Difference Test

This research aims, 1). To assess the growth and production of two black soybean varieties. 2). To examine the effect of using organic fertilizer on the growth and production of black soybean. 3). To examine the interaction between various types of organic fertilizers and varieties on the growth and production of black soybeans.This research was conducted at the practice site of the Gowa Agricultural Development Polytechnic (Polbangtan), located at Romanglompoa Village, Bontomarannu, Gowa District. This research was conducted from July to December 2018. Data obtained from observations were analysed statistically (Analysis of variance with the F test) in accordance with the design used. In this study the experiments were analysed using the Split Plot Design. If the variance shows that F count is real or very real, then the data analysis is continued with the test using the Least Significant Difference test (LSD). The results showed that Detam-3 varieties had a significantly different effect with Detam-4 on the growth and production of black soybean plants, mixed organic fertilizer (bokashi manure, rice straw, and rice husk) showed the best effect on the growth and production of black soybean, and there is no interaction between the type of organic fertilizer and soybean varieties on the growth and production of black soybean. It is recommended to use Detam-3 black soybean varieties accompanied by the use of bokashi organic fertilizer made from rice straw as part of a zero-waste farming system to support optimum production

scholarly journals Efek ekstrak etanol kedelai hitam (Glycine soja) terhadap ketebalan dermis mencit (Mus musculus L.) pasca unilateral ovariektomi

2019 ◽  
Vol 23 (2) ◽  
pp. 80
Author(s):  
Isna Kurotul Akyun ◽  
Susantin Fajariyah ◽  
Mahriani Mahriani
Keyword(s):  
Mus Musculus ◽  
Reproductive System ◽  
Glycine Soja ◽  
Steroid Hormone ◽  
Soybean Seed ◽  
Estrogen Deficiency ◽  
Female Reproductive System ◽  
Black Soybean ◽  
Unilateral Ovariectomy ◽  
Soybean Extract

Estrogen is a steroid hormone produced by the ovaries. Estrogen is a hormone that triggers collagensynthesis by fibroblast.Collagen has affects for thickness of dermis.Estrogen deficiency can lead disruptioncollagen synthesis, so has an impact on decline dermis thickness. Unilateral ovariectomy is the act ofremoval of one ovary in the female reproductive system and is a model for estrogen deficiency. Thecondition of estrogen deficiency can be overcome by giving phytoestrogens. Black soybean seed is one ofphytoestrogens source. The purpose of this study was to determine the effect of black soybean extract afterovariectomy on increasing on dermis thickness (Mus musculus L.). The dosage of black soybean ethanolextract used was 0.31 g / ml / day and 0.63 g / ml / day administered orally (gavage) for 20 days. The resultsshowed a dose of 0.31 g / ml / day may and 0.63 g / ml / day increase dermis thickness of mice.

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