scholarly journals Effect of various solvent on the specific amino acids of black soybean (Glycine soja) sprout

Author(s):  
B Kanetro ◽  
A Slamet ◽  
A Wazyka
2021 ◽  
Vol 19 (1) ◽  
pp. 35-43
Author(s):  
Awatsaya Chotekajorn ◽  
Takuyu Hashiguchi ◽  
Masatsugu Hashiguchi ◽  
Hidenori Tanaka ◽  
Ryo Akashi

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.


2017 ◽  
Vol 6 (1) ◽  
pp. 44-50
Author(s):  
Dewi M Kurniawati ◽  
Edi Dharmana ◽  
Banundari Rachmawati ◽  
Tjokorda Gde Dalem Pemayun

Background: Obese is risk factor for abnormal glucose homeostasis and causes oxidative stress that can decrease insulin sensitivity resulting in hyperglycemia and hyperinsulinemia. Black soy bean contains anthocyanins and isoflavones, it is expected to reduce oxidative stress. Objective: Determine the effect of various dosage of black soybean extract (Glycine soja.) on blood glucose, insulin levels, and HOMA - IR in obese Sprague Dawley (SD) rats.Methods: True experimental study with randomized controlled pre test-post test design in male SD rats. Twenty four samples divided into 4 groups: control (K), 500 (P1), 750 (P2), and 1000 mg/kgBW (P3). Rats were induced to become obese with High Fat Sucrose Diet (HFSD) for 4 weeks then black soybean extracts were given for 2 weeks..Results: There was no difference in blood glucose before and after treatment (p> 0.05). There were differences on insulin levels in 500 (p = 0.012) and 750mg/kgBW (p = 0.020) doseges. There were differences on HOMA-IR in 500 (p = 0.043) and 750mg/kgBW (p = 0.028) dosages. There was difference in insulin levels’s difference (p = 0.009) between groups.Conclusion: There are differences on insulin levels and HOMA – IR in 500 and 750 mg/kgBW dosages groups. However, there is no difference on blood glucose before and after black soybean extract treatment. The most decreased levels of blood glucose, insulin and HOMA- IR were in 750mg/kgBW dosage.


2003 ◽  
Vol 81 (6) ◽  
pp. 387-394 ◽  
Author(s):  
Patrick H.K Ngai ◽  
T B Ng

A monomeric protein, with a molecular mass of 25 kDa and an N-terminal sequence resembling a segment of chitin synthase, was isolated from the seeds of the black soybean Glycine soja. The protein, designated glysojanin, demonstrated potent antifungal activity against the fungi Fusarium oxysporum and Mycosphaerella arachidicola. It inhibited HIV-1 reverse transcriptase with an IC50 of 47 µmol/L, [methyl-3H]thymidine incorporation by mouse spleen cells with an IC50 of 175 µmol/L, and translation in the rabbit reticulocyte lysate with an IC50 of 20 µmol/L. Glysojanin was purified using a procedure that involved ion-exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion-exchange chromatography by fast protein liquid chromatography on Mono S, and gel filtration by fast protein liquid chromatography on Superdex 75.Key words: antifungal protein, seeds, soybean, purification.


Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1029
Author(s):  
Yu Xiao ◽  
Yuxin Huang ◽  
Yulian Chen ◽  
Ziyi Fan ◽  
Ruyang Chen ◽  
...  

In this study, black soybean was firstly processed by solid-state fermentation (SSF) with a probiotic fungus Eurotium cristatum YL-1. The effect of SSF on the nutritional components (including proximate, amino acids, minerals, and fatty acids), total phenolics, isoflavones, antioxidant activity, and volatile organic compounds of black soybeans were revealed. Results of this work demonstrated that black soybean processed by SSF with E. cristatum greatly increased the contents of protein, essential amino acids, and some minerals (e.g., calcium, phosphorus, and magnesium). GC results revealed that more than 80% of the total lipids from both fermented and non-fermented black soybeans were unsaturated fatty acids, and SSF influenced the fatty acids composition. Higher contents of total phenolics and aglycone isoflavones (i.e., genistein, daidzein, and glycitein) of fermented black soybeans were achieved by SSF. Furthermore, SSF with E. cristatum considerably augmented the ferric reducing antioxidant power, scavenging effects against ABTS·+ and DPPH radical, reducing power, and chelating ability of black soybeans, which evaluated with various polarity solvent extracts. HS-GC-IMS analysis detected a total of 66 volatile compounds in FBS and BS, and 56 volatile organic compounds were successfully identified. The intensities of main volatile compounds (i.e., 10 esters, 11 alcohols, and 19 aldehydes) differed remarkably by fermentation with E. cristatum. The intensities of seven alcohols and nine aldehydes considerably decreased, whereas higher levels of esters were achieved by SFF. Thus, our results confirmed that black soybeans processed by SSF with E. cristatum is a promising approach to substantially improve its nutritional value, flavor characteristics, and biological effect, and might have great potential in the development of new functional foods or be used as a new nutritional ingredient applied in food design.


2020 ◽  
Vol 3 (2) ◽  
pp. 129-142
Author(s):  
Muhammad Rizal ◽  
St Subaedah ◽  
Aminah Muchdar

This research aims, 1). To assess the growth and production of two black soybean varieties. 2). To examine the effect of using organic fertilizer on the growth and production of black soybean. 3). To examine the interaction between various types of organic fertilizers and varieties on the growth and production of black soybeans.This research was conducted at the practice site of the Gowa Agricultural Development Polytechnic (Polbangtan), located at Romanglompoa Village, Bontomarannu, Gowa District. This research was conducted from July to December 2018. Data obtained from observations were analysed statistically (Analysis of variance with the F test) in accordance with the design used. In this study the experiments were analysed using the Split Plot Design. If the variance shows that F count is real or very real, then the data analysis is continued with the test using the Least Significant Difference test (LSD). The results showed that Detam-3 varieties had a significantly different effect with Detam-4 on the growth and production of black soybean plants, mixed organic fertilizer (bokashi manure, rice straw, and rice husk) showed the best effect on the growth and production of black soybean, and there is no interaction between the type of organic fertilizer and soybean varieties on the growth and production of black soybean. It is recommended to use Detam-3 black soybean varieties accompanied by the use of bokashi organic fertilizer made from rice straw as part of a zero-waste farming system to support optimum production


2019 ◽  
Vol 23 (2) ◽  
pp. 80
Author(s):  
Isna Kurotul Akyun ◽  
Susantin Fajariyah ◽  
Mahriani Mahriani

Estrogen is a steroid hormone produced by the ovaries. Estrogen is a hormone that triggers collagensynthesis by fibroblast.Collagen has affects for thickness of dermis.Estrogen deficiency can lead disruptioncollagen synthesis, so has an impact on decline dermis thickness. Unilateral ovariectomy is the act ofremoval of one ovary in the female reproductive system and is a model for estrogen deficiency. Thecondition of estrogen deficiency can be overcome by giving phytoestrogens. Black soybean seed is one ofphytoestrogens source. The purpose of this study was to determine the effect of black soybean extract afterovariectomy on increasing on dermis thickness (Mus musculus L.). The dosage of black soybean ethanolextract used was 0.31 g / ml / day and 0.63 g / ml / day administered orally (gavage) for 20 days. The resultsshowed a dose of 0.31 g / ml / day may and 0.63 g / ml / day increase dermis thickness of mice.


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