Requirement of Cyanobacteria Synechococcus elongatus for the biomass and lipid production

Abstract Synechococcus elongatus is unicellularCyanobacteria having fast growth and can conduct photosynthesis process in its cells.This research purposed to evaluate the potential of Synechococcus elongatus as an candidate of biodiesel feedstocks by evaluating its biomass and lipid content in appropriate nutrients. The study was conducted at Asian Institute of Technology, Thailand. The Factorial Completely Randomized Design was used as experimental design in this study. The species was grown in BG II medium which was added by different doses of NaNO3 and KHPO4. Cultivation was conducted for seven days for several parameters, namely appropriate dose of nutrients, specific growth rate, biomass, and lipid content. This study resulted the optimal dose of nitrate from NaNO3 and phosphate from KHPO4 for good growth of Synechococcus elongatus which were 289.11 mg/L of NO3- and 22.26 mg/L of HPO4.This species grew well in BG-II medium added optimal nutrients at specific growth rate of 0.34µg/day. The optimal lipid productivity of Synechococcus elongatus was achieved at day 6th of cultivation which its dried biomass was 0.21±0.03 g/L and total lipid was 1.89±0.28%. Based on the results, Synechococcus elongatus cultivated in BG-II medium was potential tobe biodiesel source with its fast growth and lipid content in its cell.

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The effect of ethanol and specific growth rate on the lipid content and composition ofSaccharomyces cerevisiae grown anaerobically in a chemostat

Yeast ◽

10.1002/yea.320111006 ◽

1995 ◽

Vol 11 (10) ◽

pp. 953-959 ◽

Cited By ~ 29

Author(s):

Nils Arneborg ◽

Carl-Erik Høy ◽

O. B. Jørgensen

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Lipid Content ◽

Specific Growth

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Aktivitas Enzim Inulinase dan Laju Pertumbuhan Spesifik Isolat Bakteri IS-1 pada Medium Tepung Umbi Dahlia

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.485 ◽

2018 ◽

Vol 10 (2) ◽

pp. 48-55

Author(s):

Wijanarka Wijanarka ◽

Maulida Aqlia ◽

Kristina Kristina

Keyword(s):

Growth Rate ◽

Enzyme Activity ◽

Specific Growth Rate ◽

Incubation Time ◽

Specific Growth ◽

Bacterial Isolate ◽

Good Growth ◽

Production Medium ◽

Inulin Hydrolysis ◽

Medium Culture

Dahlia bulbs (Dahlia variabilis) contains carbohydrates in the form of inulin. Inulin is a form of fructose polymer from fructose monomers. Inulin hydrolysis produces a product in the form of fructose.Inulinase enzyme is an enzyme able to purely hydrolyze inulin. This study aims to determine the activity of inulinase enzymes and the specific growth rate of IS_1 bacterial isolates producing inulinase in medium of Dahlia tuber flour. Isolates of inulinase-producing bacteria were isolated directly from decay dahlia tubers and used as an inoculum in creating starter.The measurement of cell growth was carried out by inoculating a 20-hour starter on the production medium. Incubation is done for 24 hours using a rotary shaker with a speed of 120 rpm. Sampling was carried out every 4 hours at 0-hour (T0), 4 hours (T4), 8 hours (T8), 12 hours (T12), 16 hours (T16), 20 hours (T20) and 24 hours (T24) intervals with a sample of 5 mL measured for absorbance of λ 520 nm using spectrophotometer. Production medium culture in each sample was taken 1 mL and centrifuged at 3000 rpm for 10 minutes to obtain supernatant of crude enzyme. Measurement of inulinase enzyme activity was carried out using 3,5-dinitrosalicylic acid (DNS) method and spectrophotometric method at λ 570 nm. IS_1 bacterial isolate showed optimum growth at incubation time of 8 hours with OD of 0.647 and had a specific growth rate (µ) of 0.02 / hour with a generation time of 34.65 hours. The highest enzyme activity produced by IS_1 bacteria at 8 hours incubation time was 0.490 IU. The conclusion of this study is that IS_1 bacteria indicates good growth in medium of dahlia tuber flour and has inulinase enzyme activity which is able to hydrolyze inulin into fructose. Key words: inulin, bacterial isolate, inulinase, production

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PENGARUH CO2 TERHADAP LAJU PERTUMBUHAN DAN KANDUNGAN LIPID BOTRYOCOCCUS BRAUNII PADA MEDIA AIR LAUT

JURNAL GEOLOGI KELAUTAN ◽

10.32693/jgk.13.1.2015.257 ◽

2016 ◽

Vol 13 (1) ◽

Author(s):

Verina J. Wargadalam ◽

Edi Saadudin ◽

Silvy R. Fitri

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Total Lipid ◽

Specific Growth ◽

Sea Water ◽

Fatty Acid Content ◽

Lipid Production ◽

Botryococcus Braunii ◽

Cultivation Process ◽

Co2 Addition

Mikroalga sebagai salah satu opsi penyedia sumber bahan bakar nabati telah banyak diteliti. Salah satu yang perlu diperhatikan untuk keberlajutannya adalah sangat besarnya kebutuhan air yang harus disediakan dalam proses budidaya. Untuk menghindari kompetisi kebutuhan air tersebut, budidaya mikroalga dalam media air laut/salin dapat dipertimbangkan. Penelitian ini mempelajari pengaruh penambahan CO2 terhadap laju pertumbuhan dan kandungan lipid mikroalga Botryococcus braunii KÃ¼tzing strain NIES-836 yang dibudidaya dalam media salin dengan sistem terbuka. Penambahan CO2 sebesar 2% hingga 20% meningkatkan laju pertumbuhan spesifik, dengan nilai tertinggi dicapai pada penambahan 2% CO2, yaitu 0,53/hari, dan laju pertumbuhan mulai menurun signifikan pada penambahan 20% CO2. Produksi lipid tertinggi yang dapat dipulihkan juga dicapai pada penambahan 2% CO2, yaitu sebesar 0,34 g/L. Kandungan asam lemak dalam total lipid diketahui hanya mencapai 25% dengan komposisi utamanya Metil Palmitat, Metil Palmitoleat dan Metil Oleat.Kata kunci: Botryococcus braunii, CO2, laju pertumbuhan, lipid, air laut Microalgae as an alternative resource for biofuel have attracted many research works, and one of the concerns is its sustainability due to the need of a huge amount of water in the cultivation process. To avoid such water competition, cultivation of microalgae in media of saline/sea water can be considered. In this works, the effects of CO2 on the growth and lipid accumulation of Botryococcus braunii KÃ¼tzing strain NIES-836 in sea water/saline media and open system have been studied. The CO2 additions of 2% to 20% were found to increase the specific growth rate. The highest value was reached at 2% CO2 addition, i.e.: 0.53/day, whereas, the specific growth rate started to decrease significantly at 20% CO2 addition. The highest lipid production that could be recovered was 0.34 g/L, and that was observed at 2% CO2 addition. The fatty acid content in the total lipid was 25%, mainly consists of Methyl Palmitate, Methyl Palmitoleate, and Methyl Oleate. Keywords: Botryococcus braunii, CO2, growth rate, lipid, sea water

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Impact of Nitrogen Source on the Growth, Specific Growth Rate and Lipid Synthesis of Monoraphidium sp.FXY-10 under Different Culture Conditions

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.864-867.71 ◽

2013 ◽

Vol 864-867 ◽

pp. 71-78

Author(s):

Ke Ding ◽

Tao Li ◽

Peng Zhao ◽

Lin Wang ◽

Xu Ya Yu

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Nitrogen Source ◽

Lipid Content ◽

Specific Growth ◽

Lipid Synthesis ◽

Culture Conditions ◽

Optimal Conditions ◽

Maximum Biomass ◽

The Impact

The NaNO3concentration influencing the growth and lipid synthesis ofMonoraphidiumsp.FXY-10 under three cultures (autotrophy, heterotrophy and mixotrophy) was studied through flask-shaking experiment. Additionally, the impact of nitrogen source on the growth and lipid content ofMonoraphidiumsp.FXY-10 was investigated. The NaNO3concentration for maximum biomass and highest lipid content ofMonoraphidiumsp.FXY-10 was obtained and the optimal conditions for its N absorption were concluded through the Andrew model.

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Simultaneous increases in specific growth rate and specific lipid content ofChlorella vulgaristhrough UV-induced reactive species

Biotechnology Progress ◽

10.1002/btpr.1854 ◽

2013 ◽

Vol 30 (2) ◽

pp. 291-299 ◽

Cited By ~ 6

Author(s):

Ranjini Balan ◽

G. K. Suraishkumar

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Lipid Content ◽

Specific Growth ◽

Reactive Species ◽

Specific Lipid

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Strategy for Managing Industrial Anaerobic Sludge through the Heterotrophic Cultivation of Chlorella sorokiniana: Effect of Iron Addition on Biomass and Lipid Production

Bioengineering ◽

10.3390/bioengineering8060082 ◽

2021 ◽

Vol 8 (6) ◽

pp. 82

Author(s):

Esteban Charria-Girón ◽

Vanessa Amazo ◽

Daniela De Angulo ◽

Eliana Hidalgo ◽

María Francisca Villegas-Torres ◽

...

Keyword(s):

Growth Rate ◽

Specific Growth Rate ◽

Specific Growth ◽

Lipid Production ◽

Oxygen Transfer Rate ◽

Treatment Plant ◽

Acid Synthesis ◽

Chlorella Sorokiniana ◽

Anaerobic Sludge ◽

Heterotrophic Cultivation

Microalgae provides an alternative for the valorization of industrial by-products, in which the nutritional content varies substantially and directly affects microalgae system performance. Herein, the heterotrophic cultivation of Chlorella sorokiniana was systematically studied, allowing us to detect a nutritional deficiency other than the carbon source through assessing the oxygen transfer rate for glucose or acetate fermentation. Consequently, a mathematical model of the iron co-limiting effect on heterotrophic microalgae was developed by exploring its ability to regulate the specific growth rate and yield. For instance, higher values of the specific growth rate (0.17 h−1) compared with those reported for the heterotrophic culture of Chlorella were obtained due to iron supplementation. Therefore, anaerobic sludge from an industrial wastewater treatment plant (a baker’s yeast company) was pretreated to obtain an extract as a media supplement for C. sorokiniana. According to the proposed model, the sludge extract allowed us to supplement iron values close to the growth activation concentration (KFe ~12 mg L−1). Therefore, a fed-batch strategy was evaluated on nitrogen-deprived cultures supplemented with the sludge extract to promote biomass formation and fatty acid synthesis. Our findings reveal that nitrogen and iron in sludge extract can supplement heterotrophic cultures of Chlorella and provide an alternative for the valorization of industrial anaerobic sludge.

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