scholarly journals Melt electrospun fibrous architectures with target geometries

2021 ◽  
Vol 1208 (1) ◽  
pp. 012004
Author(s):  
Budimir Mijović ◽  
Josip Jelić ◽  
Petra Brać ◽  
Snježana Kirin
Keyword(s):  
Fibre Diameter ◽  
Polymer Melt ◽  
Direct Writing ◽  
Tissue Engineering Scaffolds ◽  
Electrospinning Technique ◽  
Collector Plate ◽  
Melt Electrospinning ◽  
Melt Spun ◽  
Tissue Reconstruction ◽  
2D And 3D

Abstract In the melt electrospinning technique, the polymer melt is stretched under high voltage and the cooled to form microfibers structures with a fibre diameter in the tens of micrometres range, although some studies have reported values ranging from hundreds of nanometres to hundreds of micrometres. In this respect, this technique has significance in the biomedical field, where tissue engineering scaffolds with bimodal (nano and micro) fibrous structures are preferred in regard to cell adhesion, spreading and infiltration to final tissue reconstruction. This paper gives a review of recently reported melt electrospinning devices, especially those based on the direct writing principle, and of their comparison with the new melt Spraybase electrospinning device. The Spraybase device provides high precision melt jet deposition into 2D and 3D programmed architectures, with versatile translation speeds of the collector plate in the X-Y and the melt head in the Z direction. The melt spun fibrous architectures are designed depending on the types of tissue cells used in scaffold development.

Reconstituted Extracellular Matrix Improve Osteoblastic Differentiation onto Titanium Surfaces

2012 ◽  
Vol 706-709 ◽  
pp. 584-588
Author(s):  
Lia Rimondini ◽  
Federica Demarosi ◽  
Ismaela Foltran ◽  
Nadia Quirici
Keyword(s):  
Extracellular Matrix ◽  
Electrostatic Force ◽  
Biological Tissues ◽  
Osteoblastic Differentiation ◽  
Cell Phenotype ◽  
Tissue Engineering Scaffolds ◽  
Electrospinning Technique ◽  
Differentiation Potential ◽  
Oral Implants

Electrospinning technique is an efficient processing method to manufacture micro-and nanosized fibrous structures by electrostatic force for different applications. In biomaterial field, electrospinning technique has been successfully utilized to prepare new drug delivery materials and tissue engineering scaffolds. Fiber mats of biodegradable polymers having a diameter in the nanoto submicro-scale can be considered to mimic the nanofibrous structure of native extracellular matrix (ECM). Native extracellular matrix, constituted of proteins and polysaccharides improving cells growth in its nanofibrous porous structure, controls not only the cell phenotype, but the whole structure of the biological tissues. In the present study we investigated the effect of electrospun reconstituted collagen fibers onto metals for oral implants devices manufacturing as far as the osteoblastic differentiation potential of stem cells and cytofunctionality of osteoblasts in-vitro. The cells cultured onto titanium samples coated with ECM constituents showed faster osteoblastic differentiation and more efficient deposition of mineralized matrix in comparison with those onto uncoated substrates.

scholarly journals Adsorption/desorption and biofunctional properties of oleuropein loaded on different types of silk fibroin matrices

2017 ◽  
Vol 36 (1) ◽  
Author(s):  
Oguz Bayraktar ◽  
Ali Bora Balta ◽  
Guldemet Basal Bayraktar
Keyword(s):  
Silk Fibroin ◽  
Antibacterial Properties ◽  
Average Diameter ◽  
Electrospinning Technique ◽  
Collector Plate ◽  
Regenerated Silk Fibroin ◽  
Different Types ◽  
Healing Property ◽  
Silk Fibroin Nanofibers ◽  
Adsorption Desorption

The objective of this study was to investigate the adsorption/desorption behavior of oleuropein on different types of silk fibroin matrices including silk fibroin microfibers (MF), regenerated silk fibroin (RSF), and silk fibroin nanofibers (NF). Nanofibers with an average diameter of ranging between 24 and 326 nm were successfully prepared using the electrospinning technique. The effects of the silk fibroin concentration, the voltage applied and the distance between needle tip and collector plate on the morphology of the NF were investigated. The adsorption capacities of MF, RSF and NF were determined as 104.92, 163.07 and 228.34 mg oleuropein per gram of material, respectively. The percentage of initially adsorbed oleuropein that was desorbed was 86.08, 91.29 and 96.67% for MF, RSF and NF, respectively.NF and RSF discs loaded with oleuropein were subjected to disc diffusion assays to determine their antibacterial activity against test microorganisms Staphylococcus epidermidis (Gram +) and Escherichia coli (Gram – ). The results showed that both biomaterials possessed antibacterial properties after loading with oleuropein. Wound scratch assays using oleuropein released from NF revealed an enhancement of cell migration, indicating a wound healing property of the material.In conclusion, the NF can be utilized as a biofunctional polymeric material with better performance for the adsorption and desorption of oleuropein compared with MF and RSF.

Numerical simulation of gas‐assisted polymer‐melt electrospinning: Parametric study of a multinozzle system for mass production

2020 ◽  
Vol 60 (9) ◽  
pp. 2111-2121
Author(s):  
Youbin Kwon ◽  
Jihyun Yoon ◽  
Seung‐Yeol Jeon ◽  
Daehwan Cho ◽  
Kwangjin Lee ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Mass Production ◽  
Parametric Study ◽  
Polymer Melt ◽  
Melt Electrospinning

scholarly journals Bioprinting for Liver Transplantation

2019 ◽  
Vol 6 (4) ◽  
pp. 95 ◽  
Author(s):  
Christina Kryou ◽  
Valentina Leva ◽  
Marianneza Chatzipetrou ◽  
Ioanna Zergioti
Keyword(s):  
Direct Writing ◽  
Liver Tissue Engineering ◽  
Liver Model ◽  
Cell Structures ◽  
Wide Range ◽  
Ink Jet ◽  
Functional Complex ◽  
On Chip ◽  
2D And 3D

Bioprinting techniques can be used for the in vitro fabrication of functional complex bio-structures. Thus, extensive research is being carried on the use of various techniques for the development of 3D cellular structures. This article focuses on direct writing techniques commonly used for the fabrication of cell structures. Three different types of bioprinting techniques are depicted: Laser-based bioprinting, ink-jet bioprinting and extrusion bioprinting. Further on, a special reference is made to the use of the bioprinting techniques for the fabrication of 2D and 3D liver model structures and liver on chip platforms. The field of liver tissue engineering has been rapidly developed, and a wide range of materials can be used for building novel functional liver structures. The focus on liver is due to its importance as one of the most critical organs on which to test new pharmaceuticals, as it is involved in many metabolic and detoxification processes, and the toxicity of the liver is often the cause of drug rejection.

scholarly journals Melt Electrospinning Writing Process Guided by a “Printability Number”

2017 ◽  
Vol 139 (8) ◽  
Author(s):  
Filippos Tourlomousis ◽  
Houzhu Ding ◽  
Dilhan M. Kalyon ◽  
Robert C. Chang
Keyword(s):  
Three Dimensional ◽  
Polymer Melt ◽  
Volumetric Flow Rate ◽  
Thermal Conditions ◽  
Electrospinning Process ◽  
Critical Parameters ◽  
Experimental Investigations ◽  
Dimensionless Numbers ◽  
Melt Electrospinning ◽  
Resistive Forces

The direct electrostatic printing of highly viscous thermoplastic polymers onto movable collectors, a process known as melt electrospinning writing (MEW), has significant potential as an additive biomanufacturing (ABM) technology. MEW has the hitherto unrealized potential of fabricating three-dimensional (3D) porous interconnected fibrous mesh-patterned scaffolds in conjunction with cellular-relevant fiber diameters and interfiber distances without the use of cytotoxic organic solvents. However, this potential cannot be readily fulfilled owing to the large number and complex interplay of the multivariate independent parameters of the melt electrospinning process. To overcome this manufacturing challenge, dimensional analysis is employed to formulate a “Printability Number” (NPR), which correlates with the dimensionless numbers arising from the nondimensionalization of the governing conservation equations of the electrospinning process and the viscoelasticity of the polymer melt. This analysis suggests that the applied voltage potential (Vp), the volumetric flow rate (Q), and the translational stage speed (UT) are the most critical parameters toward efficient printability. Experimental investigations using a poly(ε-caprolactone) (PCL) melt reveal that any perturbations arising from an imbalance between the downstream pulling forces and the upstream resistive forces can be eliminated by systematically tuning Vp and Q for prescribed thermal conditions. This, in concert with appropriate tuning of the translational stage speed, enables steady-state equilibrium conditions to be achieved for the printing of microfibrous woven meshes with precise and reproducible geometries.

Fibre pulsing during melt electrospinning writing

2016 ◽  
Vol 17 (3-4) ◽  
Author(s):  
Gernot Hochleitner ◽  
Almoatazbellah Youssef ◽  
Andrei Hrynevich ◽  
Jodie N. Haigh ◽  
Tomasz Jungst ◽  
...  
Keyword(s):  
Additive Manufacturing ◽  
Processing Parameters ◽  
Simple Approach ◽  
Direct Writing ◽  
Melt Electrospinning ◽  
Printing Quality ◽  
Constant Diameter ◽  
Feeding Pressure ◽  
The Stability ◽  
Acceleration Voltage

AbstractAdditive manufacturing with electrohydrodynamic direct writing is a promising approach for the production of polymeric microscale objects. In this study we investigate the stability of one such process, melt electrospinning writing, to maintain accurate placement of the deposited fibre throughout the entire print. The influence of acceleration voltage and feeding pressure on the deposited poly(ε-caprolactone) fibre homogeneity is described, and how this affects the variable lag of the jet drawn by the collector movement. Three classes of diameter instabilities were observed that led to poor printing quality: (1) temporary pulsing, (2) continuous pulsing, and (3) regular long bead defects. No breakup of the electrified jet was observed for any of the experiments. A simple approach is presented for the melt electrospinning user to evaluate fibre writing integrity, and adjust the processing parameters accordingly to achieve reproducible and constant diameter fibres.

Electrohydrodynamic quenching in polymer melt electrospinning

2011 ◽  
Vol 23 (7) ◽  
pp. 073102 ◽  
Author(s):  
Eduard Zhmayev ◽  
Daehwan Cho ◽  
Yong Lak Joo
Keyword(s):  
Polymer Melt ◽  
Melt Electrospinning

scholarly journals Hybrid Process Chain for the Integration of Direct Ink Writing and Polymer Injection Molding

Micromachines ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 509 ◽  
Author(s):  
Dario Loaldi ◽  
Leonardo Piccolo ◽  
Eric Brown ◽  
Guido Tosello ◽  
Corey Shemelya ◽  
...  
Keyword(s):  
Injection Molding ◽  
Polymer System ◽  
Polymer Melt ◽  
Hybrid Process ◽  
Direct Write ◽  
Mold Surface ◽  
Direct Writing ◽  
Process Chain ◽  
Polymer Injection ◽  
Functional Features

The integration of additive manufacturing direct-writing technologies with injection molding provides a novel method to combine functional features into plastic products, and could enable mass-manufacturing of custom-molded plastic parts. In this work, direct-write technology is used to deposit conductive ink traces on the surface of an injection mold. After curing on the mold surface, the printed trace is transferred into the plastic part by exploiting the high temperature and pressure of a thermoplastic polymer melt flow. The transfer of the traces is controlled by interlocking with the polymer system, which creates strong plastic/ink interfacial bonding. The hybrid process chain uses designed mold/ink surface interactions to manufacture stable ink/polymer interfaces. Here, the process chain is proposed and validated through systematic interfacial analysis including feature fidelity, mechanical properties, adhesion, mold topography, surface energy, and hot polymer contact angle.

Antithrombogenic properties of Tulbaghia violacea–loaded polycaprolactone nanofibers

2020 ◽  
Vol 35 (2) ◽  
pp. 102-116
Author(s):  
Lerato N Madike ◽  
Michael Pillay ◽  
Ketul C Popat
Keyword(s):  
Medical Devices ◽  
Plant Extracts ◽  
Platelet Adhesion ◽  
Blood Compatibility ◽  
Fibre Diameter ◽  
Electrospinning Technique ◽  
Implantable Medical Devices ◽  
Biomaterial Surfaces ◽  
Rate Of Failure ◽  
Novel Scaffold

A broad range of polymers have been utilized for the development of blood-contacting implantable medical devices; however, their rate of failure has raised the need for developing more hemocompatible biomaterial surfaces. In this study, a novel scaffold based on polycaprolactone incorporated with 10% and 15% (w/w) Tulbaghia violacea plant extracts were fabricated using electrospinning technique. The fabricated scaffolds were then treated with T. violacea aqueous plant extracts (100 and 1000 µg/mL) to investigate their use as interfaces for blood-contacting implants. The 10% Tvio scaffold produced the lowest mean fibre diameter (193 ± 30 nm), whereas the 15% Tvio scaffold produces the highest mean fibre diameter (538 ± 236 nm) when compared with the control polycaprolactone (275 ± 61 nm) scaffold. The number of adhered platelets was directly linked to fibre diameter and concentration of plant extract in such a way that the lowest fibre diameter scaffold (10% Tvio) inhibited platelet adhesion, whereas more platelets adhered to the scaffold with the highest fibre diameter (15% Tvio scaffolds). There was also an increase in platelet adhesion as the concentration of T. violacea was increased from 100 to 1000 µg/mL for all designed scaffolds. The improved blood compatibility demonstrated by the 10% Tvio scaffold suggests that the plant possesses antithrombogenic properties, particularly at lower concentrations.

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