Phytochemical screening and antioxidant activity of ethanolic extract and ethyl acetate fraction from basil leaf (Ocimum basilicum L.) by DPPH radical scavenging method

Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractionsaccording to compound groups.Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screenedqualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were identifiedfollowing separation by thin-layer chromatography.Results: Fraction 11 exhibited the greatest DPPH radical-scavenging activity, with an IC50 value of 6.58 μg/mL, while the fraction with the greatestantioxidant activity according to the FRAP assay was fraction 10, with a ferric ion equivalent antioxidant activity value of 1015.34 μmol/g.Conclusion: Compound group identification revealed that Fractions 10 and 11 contained flavonoids, with two common to both fractions, whilefraction 10 also contained one specific flavonoid.

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Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

Journal of the Serbian Chemical Society ◽

10.2298/jsc110621183r ◽

2012 ◽

Vol 77 (4) ◽

pp. 423-435 ◽

Cited By ~ 9

Author(s):

Tauheeda Riaz ◽

Athar Abbasi ◽

A Aziz-Ur-Rehman ◽

Tayyaba Shahzadi ◽

Muhammad Ajaib ◽

...

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Ethyl Acetate ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Total Antioxidant Activity ◽

Phytochemical Screening ◽

Dpph Radical ◽

Dodonaea Viscosa ◽

Total Antioxidant

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ? 1.38% inhibition of DPPH radical at a concentration of 60 ?g/ml. The IC50 of this fraction was found to be 33.95 ? 0.58 ?g/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ? 0.89 ?g/mL. It also showed highest FRAP value (380.53 ? 0.74 ?M of trolox equivalents) as well as highest total phenolic contents (208.58 ? 1.83 GAE ?g/g) and highest value of inhibition of lipid peroxidation (58.11 ? 1.49% at concentration of 500 ?g/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ? 0.59 (eq. to BHT).

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COMPARATIVE ANALYSIS OF PHYTOCHEMICAL, ANTIBACTERIAL, AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF AZADIRACHTA INDICA LEAVES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i3.29286 ◽

2019 ◽

pp. 151-154

Author(s):

GAURAV SHARMA ◽

ANKITA THAKUR ◽

SOHAN LAL ◽

ROHIT KUMAR NADDA

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Azadirachta Indica ◽

Comparative Evaluation ◽

Ethyl Acetate Extract ◽

Radical Scavenging ◽

Dpph Radical ◽

Bacterial Strains ◽

Dpph Radical Scavenging ◽

Radical Scavenging Assay

Objective: The objective of the present study was the analysis of phytochemicals in various extracts of Azadirachta indica leaves, comparative evaluation of antibacterial activity of the various extracts of A. indica leaves against Escherichia coli and Staphylococcus aureus, and comparative evaluation of antioxidant activity in various extracts of A. indica leaves using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Methods: Various extracts were prepared by crushing the samples. Antibacterial susceptibility test, various phytochemical tests for qualitative analysis, and DPPH radical scavenging assay for antioxidant activity were performed. Results: The result suggested that alkaloids, flavonoids, and terpenoids were present in all the four extracts. Tannins were absent in the ethyl acetate extract, and phenols were only present in the ethyl acetate extract. Sterols and phlobatannins were absent in all the four extracts. Saponins were only present in the aqueous extract, and amino acids were only present in the ethyl acetate extract. The bacterial strains S. aureus and E. coli were used against the different extracts of A. indica leaves, i.e., methanol, chloroform, ethyl acetate, and aqueous. Conclusion: The results suggested that bioactive compounds found in leaves of A. indica contribute to its pharmacological activities.

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IN VITRO SCREENING OF ANTIOXIDANT AND ANTIAGING POTENTIAL OF CUCUMIS SATIVUS FRUIT EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i5.37258 ◽

2020 ◽

pp. 187-190

Author(s):

CHANCHAL GARG ◽

RAVINDER SINGH ◽

MUNISH GARG

Keyword(s):

Ethyl Acetate ◽

Cucumis Sativus ◽

Inhibitory Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Positive Control ◽

Dpph Radical ◽

Dpph Radical Scavenging

Objective: The present study was designed to screen the anti-aging and anti-wrinkle potential of Cucumis sativus fruit through in vitro estimation of antioxidant, anti-hyaluronidase, anti-elastase, anti-collagenase/anti-matrix metalloproteinase (MMP)-1, and anti-tyrosinase activity. Methods: Raw juice of cucumber was taken, filtered and fractionated with ethyl acetate and n-butanol. The obtained extracts were then evaluated for their antioxidant potential through 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay taking ascorbic acid as positive control and other enzymatic activities in reference to hyaluronidase inhibition, MMP-1/collagenase inhibition, and elastase inhibition taking catechin as reference standard whereas for tyrosinase inhibition the standard used was quercetin. Results: All the evaluations were performed in triplicates and results were noted down. It was observed that aqueous extract of C. sativus fruits showed a maximum DPPH radical scavenging activity (p<0.0001), half-maximal inhibitory concentration (IC50) at a concentration of 122.67 μg/ml. The ethyl acetate fraction of C. sativus fruits exhibited maximum hyaluronidase (p<0.0001), MMP-1/collagenase (p<0.04), and tyrosinase (p<0.04) inhibitory activity, IC50 at a concentration of 59.54, 45.79, and 24.46 μg/ml, respectively. The elastase (p<0.0001) inhibitory activity by n-butanol fraction of C. sativus fruits extract was maximum, IC50 at a concentration of 52.76 μg/ml. Conclusion: A potent anti-aging and anti-wrinkle properties were well demonstrated by C. sativus, as depicted from the results obtained.

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PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF MILLINGTONIA HORTENSIS (L)

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2017v9i5.2217 ◽

2017 ◽

pp. 162-167

Author(s):

Janaki A. ◽

Kaleena P. K. ◽

Elumalai D. ◽

Hemalatha P. ◽

Babu M. ◽

...

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Antibacterial Activities ◽

Phytochemical Screening ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Leaf Extracts ◽

Micro Organisms ◽

Dpph Radical Scavenging

Objective: Millingtonia hortensis Linn (Bignoniaceae) is commonly known as cork tree and Akash neem. Aim of studies to determine the antioxidant activity and antibacterial activity.Methods: The antioxidant activity of different solvent extracts were measured by chemical analyses involving the assay of 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and super oxide radical scavenging activity.Results: Phytochemicals (secondary metabolites) screening of methanol, chloroform, ethanol, petroleum ether, aqueous leaf extracts revealed the presence of carbohydrates, tannins, saponins, flavonoids, alkaloids, betacyanins, phenols and coumarins.Conclusion: The presence of these phytochemicals and antioxidant capacity support the use of this plant as an antibacterial agent against the group of micro organisms tested.Â

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ANTIOXIDATIVE ACTIVITIES AND CHEMICAL CONSTITUENTS OF THE ETHYL ACETATE EXTRACT FROM JASMINUM UNDULATUM KER-GAWL

Science and Technology Development Journal ◽

10.32508/stdj.v14i2.1943 ◽

2011 ◽

Vol 14 (2) ◽

pp. 50-57

Author(s):

Anh Thi Tran ◽

Binh Thanh Nguyen ◽

Hoai Thi Cam Ho ◽

Huong Dang Thien Bui ◽

Mai Thi Thanh Nguyen

Keyword(s):

Nitric Oxide ◽

Ethyl Acetate ◽

Petroleum Ether ◽

Antioxidative Activity ◽

Chemical Constituents ◽

Radical Scavenging ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Dpph Radical ◽

Dpph Radical Scavenging

From the total crude ethanol extract of Jasminum undulatum Ker Gawl.’s leaves and stems, five fractionss were obtained by partitioning with petroleum ether, chloroform, ethyl acetate and n-butanol solvents. These five fractions were investigated for antioxidative activity using the DPPH radical scavenging and nitric oxide-inhibitory assay. All the fractions showed antioxidative activity except the petroleum ether fraction. Among the fractionss, the ethyl acetate fraction was the most potent fraction in both assays with the SC50 values of 5.30 μg/ml and 80.90 μg/ml, respectively. Further investigation on the eight sub-fractions isolated and extracted from the ethyl acetate fraction showed that one of these sub-fractions, the TE6 sub-fraction, showed the most significant antioxidative activity with the SC50 values of 3.15 μg/ml and 61.83 μg/ml respectively in the DPPH radical scavenging and nitric oxide-inhibitory assay. From the TE4 and TE6 sub-fractions, three compounds were isolated, including p-tyrosol (1), protocatechuic acid (2) and hydroxytyrosol (3). The structure of those compounds were elucidated by spectrometric methods IR, MS, 1D-NMR, and 2D-NMR.

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Phytochemical Investigation and In Vitro Antioxidant Activities of Tetracarpidium conophorum (African Walnut) Seeds

Avicenna Journal of Medical Biochemistry ◽

10.15171/ajmb.2018.12 ◽

2018 ◽

Vol 6 (2) ◽

pp. 56-61

Author(s):

Kelly Oriakhi ◽

Kissinger Orumwensodia ◽

Patrick Uadia

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Methanol Extract ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Phytochemical Screening ◽

Dpph Radical ◽

Dpph Radical Scavenging ◽

Crude Methanol Extract

Background: Tetracarpidium conophorum (African walnut) is an African plant with ethnobotanical uses. Objectives: The purpose of this study was to evaluate the phytochemical screening and in vitro antioxidant activities of methanol extract and fractions (F) [n-hexane (HEX-F), dichloromethane (DCM-F) and, ethyl acetate (EA-F)] of T. conophorum seeds. Methods: Phytochemical screening and in vitro antioxidant activity study were carried out using DPPH, ABTs radical scavenging assays, nitric oxide inhibitory and reducing potential assays. Results: Methanol extract and its fractions contain phenols, flavonoids, saponins, tannins, terpenoids, and alkaloids. The concentrations of total phenols and flavonoids content were significantly higher in EA-F and crude methanol extract compared to other fractions. Crude methanol and EA-F contain higher concentrations of tannin while hexane fraction had the lowest tannin content but relatively higher proanthocyanidin content compared to other fractions. The antioxidant activity study showed that both methanol crude extract and fractions of T. conophorum seeds have significant activities for DPPH radical scavenging, reducing power, ferric reducing antioxidant potential, nitric oxide inhibitory activities, ABTS and hydroxyl radical scavenging for. DPPH radical scavenging activities of EA-F showed the lowest IC50 of 33.11 µg/mL, followed by Hex-F, DCM-F and crude methanol extract with IC50 of 33.43, 42.09 and 45.44 µg/mL, respectively, when compared to ascorbic acid with IC50 of 17.08 µg/mL. Conclusion: The study showed that T. conophorum seed is a rich source of secondary metabolites, which may be responsible for its antioxidant activities.

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Antioxidant Activity of Ethanolic Extract from Tandui Leaves (Mangifera rufocostata Kosterm.) by DPPH Radical Scavenging Method

Borneo Journal of Pharmacy ◽

10.33084/bjop.v2i2.1070 ◽

2019 ◽

Vol 2 (2) ◽

pp. 114-118

Author(s):

Revita Saputri ◽

Tia Mariati Risma Melati ◽

Fitriyanti Fitriyanti

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Dpph Radical ◽

Ic50 Value ◽

Dpph Radical Scavenging

Tandui (Mangifera rufocostata Kosterm.) is a typical plant from South Kalimantan which belongs to the genus of Mangifera. Several species of Mangifera are known to have antioxidant activity. This study aimed to determine the antioxidant activity of ethanol extract of Tandui leaves. Tandui leaves that were obtained from the maceration method used 70% ethanol. Antioxidant activity was conducted quantitative using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. The result of the antioxidant activity of Ethanol extract of Tandui leaves quantitatively obtained IC50 value was 60.7042 �g/mL. The ethanol extract of Tandui leaves has strong antioxidant activity

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PENENTUAN TOTAL FENOLIK DAN AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DARI TEPUNG PELEPAH AREN (Arenga pinnata)

JURNAL ILMIAH SAINS ◽

10.35799/jis.16.2.2016.14067 ◽

2016 ◽

Vol 16 (2) ◽

pp. 68

Author(s):

Yudhie E Prasetyo ◽

Meiske S Sangi ◽

Audy D Wuntu

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Ic50 Value ◽

Dpph Radical Scavenging

PENENTUAN TOTAL FENOLIK DAN AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DARI TEPUNG PELEPAH AREN (Arenga pinnata) ABSTRAK Tujuan penelitian ini untuk menentukan total fenolik dan aktivitas antioksidan fraksi etil asetat dari tepung pelepah aren (Arenga pinnata). Tepung pelepah aren diekstraksi maserasi menggunakan etanol hasil redestilasi cap tikus kemudian di partisi menggunakan pelarut petroleum eter, etil asetat dan aquades. Penentuan kandungan total fenolik menggunakan metode Folin-Ciocalteu dan aktivitas antioksidan menggunakan metode serapan radikal 1,1-difenil-2-pikrilhidrazil (DPPH). Didapati kandungan total fenolik fraksi etil asetat sebesar 172.857 ± 4.762 mg/kg dan aktivitas penangkal radikal bebas fraksi etil asetat sebesar 90.573 µg/mL. Hasil ini menunjukan bahwa fraksi etil asetat berpotensi sebagai antioksidan alami. Kata kunci: Tepung Pelepah aren, fenolik, aktivitas antioksidan, DPPH DETERMINATION TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY ETHYL ACETATE FRACTION OF AREN’S MIDRIB POWDER (Arenga pinnata) ABSTRACT This research aims to determine the total phenolic content and antioxidant activity from ethyl acetate fraction of Aren’s midrib powder (Arenga pinnata). Aren’s midrib powder extracted with maceration method with cap tikus redistillated ethanol and then partitioned with solvents such as petroleum ether, ethyl acetate and distillate water. The determination of phenolic content was using Folin-Ciocalteu method and the antioxidant activity was done using diphenylpycrylhydrazyl (DPPH) radical scavenging. Total phenolic value of ethyl acetate fraction is 172.857 ± 4.762 and IC50 value of ethyl acetate fraction is 90.573 µg/mL. This result shows ethyl acetate fraction as natural antioxidant potential. Keywords: Aren’s midrib powder, phenolic, antioxidant activity, DPPH

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Antioxidant Potency of Red Dragon Fruit Flesh and Peel Prepared by Different Methods

Current Nutrition & Food Science ◽

10.2174/1573401316666191216124950 ◽

2020 ◽

Vol 16 (7) ◽

pp. 1106-1111

Author(s):

Novi Febrianti ◽

Purwanti P. Purbosari ◽

Triana Hertiani ◽

Sukarti Moeljopawiro ◽

Sofia M. Haryana

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Total Phenolic ◽

Dpph Radical ◽

Fruit Peel ◽

Dry Extract ◽

Dragon Fruit ◽

Dpph Radical Scavenging

Background: Red dragon fruit (Hylocereus polyrhizus, (F.A.C. Weber) Britton & Rose) is widely consumed all over the world nowadays. The peel and flesh of red dragon fruit contain many bioactive compounds with high antioxidant activity. The preparation process is critical to maximizing the yield of the antioxidant content. Objective: The objectives of this research were to evaluate total phenolic content (TPC), total flavonoid content (TFC), as well as the antioxidant activity of peel and flesh of red dragon fruit prepared by various methods. Methods: The fresh and dried samples of peel and flesh of red dragon fruit were prepared via maceration and non-maceration process. Ethanol (96%) was used as the solvent in maceration. In the nonmaceration process, the samples were ground using a blender and pressed using a juicer. TPC was analyzed by Folin-Ciocalteau methods, while TFC was determined by spectrophotometry UV-Vis with AlCl3. Antioxidant activity was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene bleaching (BCB) tests. Results: TPC from all of the measured samples varied from 22.43 ± 0.27 to 80.54 ± 0.43 mg GAE/g dry extract. The highest TPC concentration was found in the blended peel via maceration and the lowest concentration was found in the blended flesh without maceration. The dried peel via maceration treatment had the highest TFC (51.96 ± 0.084 mg of QE/g dry extract). Regarding to the antioxidant activity, the blended flesh ethanolic extract and blended peel ethanolic extract had the highest DPPH radical scavenging, IC50=966.83 ± 11.62 and 973.81 ± 3.571ppm, respectively. While the blended peel ethanolic extract had the highest BCB antioxidant activity (IC50= 45.48 ± 6,79 mg/mL). Conclusion: Preparation methods affect the antioxidant activity of red dragon fruit peel and flesh. The highest TPC and antioxidant activity (BCB test) can be found in the ethanolic extract of the blended peel. The highest TFC can be found in the ethanolic extract of dried-peel. Both the ethanolic extracts, blended peel and blended flesh, had the same DPPH radical scavenging activity.

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