Engineering of brain-like tissue constructs via 3D Cell-printing technology

2020 ◽  
Vol 12 (3) ◽  
pp. 035016 ◽  
Author(s):  
Yu Song ◽  
Xiaolei Su ◽  
Kevin F. Firouzian ◽  
Yongcong Fang ◽  
Ting Zhang ◽  
...  
Keyword(s):  
Printing Technology ◽  
Cell Printing ◽  
Tissue Constructs

scholarly journals Noninvasive in vivo 3D bioprinting

2020 ◽  
Vol 6 (23) ◽  
pp. eaba7406 ◽  
Author(s):  
Yuwen Chen ◽  
Jiumeng Zhang ◽  
Xuan Liu ◽  
Shuai Wang ◽  
Jie Tao ◽  
...  
Keyword(s):  
3D Printing ◽  
Near Infrared ◽  
Clinical Medicine ◽  
Application Site ◽  
3D Bioprinting ◽  
Printing Technology ◽  
3D Printing Technology ◽  
Tissue Constructs

Three-dimensional (3D) printing technology has great potential in advancing clinical medicine. Currently, the in vivo application strategies for 3D-printed macroscale products are limited to surgical implantation or in situ 3D printing at the exposed trauma, both requiring exposure of the application site. Here, we show a digital near-infrared (NIR) photopolymerization (DNP)–based 3D printing technology that enables the noninvasive in vivo 3D bioprinting of tissue constructs. In this technology, the NIR is modulated into customized pattern by a digital micromirror device, and dynamically projected for spatially inducing the polymerization of monomer solutions. By ex vivo irradiation with the patterned NIR, the subcutaneously injected bioink can be noninvasively printed into customized tissue constructs in situ. Without surgery implantation, a personalized ear-like tissue constructs with chondrification and a muscle tissue repairable cell-laden conformal scaffold were obtained in vivo. This work provides a proof of concept of noninvasive in vivo 3D bioprinting.

scholarly journals Coaxial nozzle-assisted electrohydrodynamic printing for microscale 3D cell-laden constructs

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Hongtao Liang ◽  
Jiankang He ◽  
Jinke Chang ◽  
Bing Zhang ◽  
Dichen Li
Keyword(s):  
Feeding Rate ◽  
Encapsulated Cells ◽  
Precise Control ◽  
Cell Printing ◽  
Tissue Constructs ◽  
Coaxial Nozzle ◽  
Electrohydrodynamic Printing ◽  
Critical Process Parameters ◽  
Critical Process ◽  
Unique Capability

Cell printing has found wide applications in biomedical fields due to its unique capability in fabricating living tissue constructs with precise control over cell arrangements. However, it is still challenging to print cell-laden 3D structures simultaneously with high resolution and high cell viability. Here a coaxial nozzle-assisted electrohydrodynamic cell printing strategy was developed to fabricate living 3D cell-laden constructs. Critical process parameters such as feeding rate and stage moving speed were evaluated to achieve smaller hydrogel filaments. The effect of CaCl2 feeding rate on the printing of 3D alginate hydrogel constructs was also investigated. The results indicated that the presented strategy can print 3D hydrogel structures with relatively uniform filament dimension (about 80 μm) and cell distribution. The viability of the encapsulated cells was over 90%. We envision that the coaxial nozzle-assisted electrohydrodynamic printing will become a promising cell printing strategy to advance biomedical innovations.

scholarly journals Maturation and Protection Effect of Retinal Tissue-Derived Bioink for 3D Cell Printing Technology

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 934
Author(s):  
Jongmin Kim ◽  
Jeong Sik Kong ◽  
Hyeonji Kim ◽  
Wonil Han ◽  
Jae Yon Won ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Retinal Degeneration ◽  
Vision Loss ◽  
Model Development ◽  
Printing Technology ◽  
Cell Printing ◽  
Retinal Tissue ◽  
Regeneration Medicine

Retinal degeneration is a leading cause of incurable vision loss and blindness. The increasing incidence of retinal degeneration has triggered research into the development of in vitro retinal models for drug development and retinal alternatives for transplantation. However, the complex retinal structure and the retinal microenvironment pose serious challenges. Although 3D cell printing technology has been widely used in tissue engineering, including in vitro model development and regeneration medicine, currently available bioinks are insufficient to recapitulate the complex extracellular matrix environment of the retina. Therefore, in this study, we developed a retinal decellularized extracellular matrix (RdECM) from the porcine retina and evaluated its characteristics. The RdECM conserved the ECM components from the native retina without cellular components. Then, we mixed the RdECM with collagen to form a bioink and confirmed its suitability for 3D cell printing. We further studied the effect of the RdECM bioink on the differentiation of Muller cells. The retinal protective effect of the RdECM bioink was confirmed through a retinal degeneration animal model. Thus, we believe that the RdECM bioink is a promising candidate for retinal tissue engineering.

Complex heterogeneous tissue constructs containing multiple cell types prepared by inkjet printing technology

Biomaterials ◽  
2013 ◽  
Vol 34 (1) ◽  
pp. 130-139 ◽  
Author(s):  
Tao Xu ◽  
Weixin Zhao ◽  
Jian-Ming Zhu ◽  
Mohammad Z. Albanna ◽  
James J. Yoo ◽  
...  
Keyword(s):  
Inkjet Printing ◽  
Cell Types ◽  
Printing Technology ◽  
Tissue Constructs ◽  
Multiple Cell ◽  
Heterogeneous Tissue ◽  
Inkjet Printing Technology

4D printing technology, modern era: A short review

2020 ◽  
pp. 92-111
Author(s):  
Khodadad Mostakim ◽  
Nahid Imtiaz Masuk ◽  
Md. Rakib Hasan ◽  
Md. Shafikul Islam
Keyword(s):  
Smart Materials ◽  
Computer Aided Design ◽  
Short Review ◽  
Stimuli Responsive ◽  
4D Printing ◽  
Space Applications ◽  
Printing Technology ◽  
Practical Applications ◽  
Biomedical Technologies ◽  
Novel Material

The advancement in 3D printing has led to the rapid growth of 4D printing technology. Adding time, as the fourth dimension, this technology ushered the potential of a massive evolution in fields of biomedical technologies, space applications, deployable structures, manufacturing industries, and so forth. This technology performs ingenious design, using smart materials to create advanced forms of the 3-D printed specimen. Improvements in Computer-aided design, additive manufacturing process, and material science engineering have ultimately favored the growth of 4-D printing innovation and revealed an effective method to gather complex 3-D structures. Contrast to all these developments, novel material is still a challenging sector. However, this short review illustrates the basic of 4D printing, summarizes the stimuli responsive materials properties, which have prominent role in the field of 4D technology. In addition, the practical applications are depicted and the potential prospect of this technology is put forward.

Effect of ink and paperboard characteristics on flexographic print quality based on print density

TAPPI Journal ◽  
2011 ◽  
Vol 10 (9) ◽  
pp. 7-13
Author(s):  
KHODADAD MALMIRCHEGINI ◽  
FARSHAD SARKHOSH RAHMANI
Keyword(s):  
Water Absorption ◽  
Significant Influence ◽  
Particle Diameter ◽  
Print Quality ◽  
Printing Technology ◽  
Plastic Films ◽  
Printing Inks ◽  
Packaging Industry ◽  
Solids Content ◽  
Density Results

Flexography is an evolving printing technology that is suitable for printing on coated and uncoated paperboard and board, nonporous substrates including metalized and paperboard foils, and plastic films used especially in the packaging industry. This study evaluated the effect of paperboard and ink characteristics on flexographic print density in paperboard. Three commercial paperboards from different companies were prepared: brown kraft from Thailand, white kraft from Spain, and test liner from Iran. Four samples of process print inks from Iran were used in this investigation. Paperboard properties, such as roughness and water absorption, and ink characteristics, including solids content, PH and particle diameter, were measured. The inks were printed on paperboards using a roll no.15 applicator with a blade metering device, and the print densities were measured. Results showed that solids content, pH, and particle diameter of printing inks influenced print density, while the roughness and water absorption of the three types of paperboard had no significant influence on print density. Results also illustrated that two levels of ink viscosity (25–30 and 50–55 mPa·s) were insignificant to print density.

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