Noninvasive in vivo 3D bioprinting

Three-dimensional (3D) printing technology has great potential in advancing clinical medicine. Currently, the in vivo application strategies for 3D-printed macroscale products are limited to surgical implantation or in situ 3D printing at the exposed trauma, both requiring exposure of the application site. Here, we show a digital near-infrared (NIR) photopolymerization (DNP)–based 3D printing technology that enables the noninvasive in vivo 3D bioprinting of tissue constructs. In this technology, the NIR is modulated into customized pattern by a digital micromirror device, and dynamically projected for spatially inducing the polymerization of monomer solutions. By ex vivo irradiation with the patterned NIR, the subcutaneously injected bioink can be noninvasively printed into customized tissue constructs in situ. Without surgery implantation, a personalized ear-like tissue constructs with chondrification and a muscle tissue repairable cell-laden conformal scaffold were obtained in vivo. This work provides a proof of concept of noninvasive in vivo 3D bioprinting.

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Role of in situ added cellulose nanocrystals as rheological modulator of novel waterborne polyurethane urea for 3D-printing technology

Cellulose ◽

10.1007/s10570-021-03826-6 ◽

2021 ◽

Author(s):

Julen Vadillo ◽

Izaskun Larraza ◽

Tamara Calvo-Correas ◽

Nagore Gabilondo ◽

Christophe Derail ◽

...

Keyword(s):

3D Printing ◽

Cellulose Nanocrystals ◽

Waterborne Polyurethane ◽

Printing Technology ◽

3D Printing Technology

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3D Printing and NIR Fluorescence Imaging Techniques for the Fabrication of Implants

Materials ◽

10.3390/ma13214819 ◽

2020 ◽

Vol 13 (21) ◽

pp. 4819

Author(s):

Yong Joon Suh ◽

Tae Hyeon Lim ◽

Hak Soo Choi ◽

Moon Suk Kim ◽

Sang Jin Lee ◽

...

Keyword(s):

3D Printing ◽

Fluorescence Imaging ◽

Near Infrared ◽

Imaging Techniques ◽

The Body ◽

Monitoring Method ◽

Printing Technology ◽

3D Printing Technology ◽

Nir Fluorescence ◽

Printing Techniques

Three-dimensional (3D) printing technology holds great potential to fabricate complex constructs in the field of regenerative medicine. Researchers in the surgical fields have used 3D printing techniques and their associated biomaterials for education, training, consultation, organ transplantation, plastic surgery, surgical planning, dentures, and more. In addition, the universal utilization of 3D printing techniques enables researchers to exploit different types of hardware and software in, for example, the surgical fields. To realize the 3D-printed structures to implant them in the body and tissue regeneration, it is important to understand 3D printing technology and its enabling technologies. This paper concisely reviews 3D printing techniques in terms of hardware, software, and materials with a focus on surgery. In addition, it reviews bioprinting technology and a non-invasive monitoring method using near-infrared (NIR) fluorescence, with special attention to the 3D-bioprinted tissue constructs. NIR fluorescence imaging applied to 3D printing technology can play a significant role in monitoring the therapeutic efficacy of 3D structures for clinical implants. Consequently, these techniques can provide individually customized products and improve the treatment outcome of surgeries.

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Current achievements in 3D bioprinting technology of chitosan and its hybrids

New Journal of Chemistry ◽

10.1039/d1nj01497h ◽

2021 ◽

Author(s):

Shadpour Mallakpour ◽

Fariba Sirous ◽

Chaudhery Mustansar Hussain

Keyword(s):

Additive Manufacturing ◽

3D Printing ◽

Three Dimensional ◽

3D Bioprinting ◽

Printing Technology ◽

3D Printing Technology ◽

The World

In recent years, additive manufacturing, or in other words three-dimensional (3D) printing technology has rapidly become one of the hot topics in the world. Among the vast majority of materials,...

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In Vitro and In Vivo Bioequivalence Study of 3D-Printed Instant-Dissolving Levetiracetam Tablets and Subsequent Personalized Dosing for Chinese Children Based on Physiological Pharmacokinetic Modeling

Pharmaceutics ◽

10.3390/pharmaceutics14010020 ◽

2021 ◽

Vol 14 (1) ◽

pp. 20

Author(s):

Xianfu Li ◽

En Liang ◽

Xiaoxuan Hong ◽

Xiaolu Han ◽

Conghui Li ◽

...

Keyword(s):

3D Printing ◽

Pbpk Model ◽

Dose Range ◽

Chinese Children ◽

Pbpk Modeling ◽

Printing Technology ◽

3D Printing Technology ◽

Pbpk Models ◽

3D Printed

Recently, the development of Binder Jet 3D printing technology has promoted the research and application of personalized formulations, which are especially useful for children’s medications. Additionally, physiological pharmacokinetic (PBPK) modeling can be used to guide drug development and drug dose selection. Multiple technologies can be used in combination to increase the safety and effectiveness of drug administration. In this study, we performed in vivo pharmacokinetic experiments in dogs with preprepared 3D-printed levetiracetam instant-dissolving tablets (LEV-IDTs). Bioequivalence analysis showed that the tablets were bioequivalent to commercially available preparations (Spritam®) for dogs. Additionally, we evaluated the bioequivalence of 3D-printed LEV-IDTs with Spritam® by a population-based simulation based on the established PBPK model of levetiracetam for Chinese adults. Finally, we established a PBPK model of oral levetiracetam in Chinese children by combining the physiological parameters of children, and we simulated the PK (pharmacokinetics) curves of Chinese children aged 4 and 6 years that were administered the drug to provide precise guidance on adjusting the dose according to the effective dose range of the drug. Briefly, utilizing both Binder jet 3D printing technology and PBPK models is a promising route for personalized drug delivery with various age groups.

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3D Bioprinting at the Frontier of Regenerative Medicine, Pharmaceutical, and Food Industries

Frontiers in Medical Technology ◽

10.3389/fmedt.2020.607648 ◽

2021 ◽

Vol 2 ◽

Author(s):

Qasem Ramadan ◽

Mohammed Zourob

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Regenerative Medicine ◽

Production Process ◽

Paradigm Shift ◽

3D Bioprinting ◽

Printing Technology ◽

Food Industries ◽

3D Printing Technology ◽

Key Driver

3D printing technology has emerged as a key driver behind an ongoing paradigm shift in the production process of various industrial domains. The integration of 3D printing into tissue engineering, by utilizing life cells which are encapsulated in specific natural or synthetic biomaterials (e.g., hydrogels) as bioinks, is paving the way toward devising many innovating solutions for key biomedical and healthcare challenges and heralds' new frontiers in medicine, pharmaceutical, and food industries. Here, we present a synthesis of the available 3D bioprinting technology from what is found and what has been achieved in various applications and discussed the capabilities and limitations encountered in this technology.

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Application of 3D Printing Technology in Bone Tissue Engineering: A Review

Current Drug Delivery ◽

10.2174/1567201817999201113100322 ◽

2020 ◽

Vol 17 ◽

Author(s):

Yashan Feng ◽

Shijie Zhu ◽

Di Mei ◽

Jiang Li ◽

Jiaxiang Zhang ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Bone Defects ◽

3D Bioprinting ◽

Printing Technology ◽

Bone Defect Repair ◽

3D Printing Technology ◽

Defect Repair

: Clinically, the treatment of bone defects remains a significant challenge, as it requires autogenous bone grafts or bone graft substitutes. However, the existing biomaterials often fail to meet the clinical requirements in terms of structural support, bone induction and controllable biodegradability. In the treatment of bone defects, 3D porous scaffolds have at-tracted much attention in the orthopedic field. In terms of appearance and microstructure, complex bone scaffolds created by 3D printing technology are similar to human bone. On this basis, the combination of active substances including cells and growth factors is more conducive to bone tissue reconstruction, which is of great significance for the personalized treatment of bone defects. With the continuous development of 3D printing technology, it has been widely used in bone defect repair as well as diagnosis and rehabilitation, creating an emerging industry with excellent market potential. Meanwhile, the di-verse combination of 3D printing technology with multi-disciplinary fields such as tissue engineering, digital medicine, and materials science has made 3D printing products with good biocompatibility, excellent osteo-inductive capacity and stable mechanical properties. In the clinical application of the repair of bone defects, various biological materials and 3D printing methods have emerged to make patient-specific bioactive scaffolds. The microstructure of 3D printed scaffolds can meet the complex needs of bone defect repair and support the personalized treatment of patients. Some of the new materials and technologies that emerged from the 3D printing industry's advent in the past decade successfully translated into clinical practice. In this article, we first introduced the development and application of different types of materials that were used in 3D bioprinting, including metal, ceramic materials, polymer materials, composite materials, and cell tissue. The combined application of 3D bioprinting and other manufacturing method used for bone tissue engineering are also discussed in this ar-ticle. Finally, we discussed the bottleneck of 3D bioprinting technique and forecasted its research orientation and prospect.

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Rapid manufacturing of micro-drilling devices using FFF-type 3D printing technology

Scientific Reports ◽

10.1038/s41598-021-91149-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Sangyeun Park ◽

Byeongjo Ko ◽

Heewon Lee ◽

Hongyun So

Keyword(s):

3D Printing ◽

Three Dimensional ◽

Dynamics Simulation ◽

Drilling Depth ◽

Printing Technology ◽

3D Printing Technology ◽

Drilling Performance ◽

Micro Drilling ◽

Fluidic Resistance

AbstractMicro-drilling devices with different blade shapes were fabricated with a rapid and facile manufacturing process using three-dimensional (3D) printing technology. The 3D-printed casting mold was utilized to customize the continuous shape of the blades without the need for expensive manufacturing tools. A computational fluid dynamics simulation was performed to estimate the pressure differences (fluidic resistance) around each rotating device in a flowing stream. Three types of blades (i.e., 45°, 0°, and helical type) were manufactured and compared to a device without blades (i.e., plain type). As a result, the device with the 45° blades exhibited the best drilling performance. At a rotational speed of 1000 rpm, the average drilling depth of the device with the 45° blades to penetrate artificial thrombus for 90 s was 3.64 mm, which was ~ 2.4 times longer than that of helical blades (1.51 mm). This study demonstrates the feasibility of using 3D printing to fabricate microscale drilling devices with sharp blades for various applications, such as in vivo microsurgery and clogged water supply tube maintenance.

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Vascularized Bone Regeneration Accelerated by 3D-Printed Nanosilicate-Functionalized Polycaprolactone Scaffold

Regenerative Biomaterials ◽

10.1093/rb/rbab061 ◽

2021 ◽

Author(s):

Xiongcheng Xu ◽

Long Xiao ◽

Yanmei Xu ◽

Jin Zhuo ◽

Xue Yang ◽

...

Keyword(s):

3D Printing ◽

Bone Regeneration ◽

Bone Repair ◽

Cytokine Secretion ◽

Printing Technology ◽

3D Printing Technology ◽

3D Printed ◽

Vascularized Bone

Abstract Critical oral-maxillofacial bone defects, damaged by trauma and tumors, not only affect the physiological functions and mental health of patients but are also highly challenging to reconstruct. Personalized biomaterials customized by 3D printing technology have the potential to match oral-maxillofacial bone repair and regeneration requirements. Laponite nanosilicates have been added to biomaterials to achieve biofunctional modification owing to their excellent biocompatibility and bioactivity. Herein, porous nanosilicate-functionalized polycaprolactone (PCL/LAP) was fabricated by 3D printing technology, and its bioactivities in bone regeneration were investigated in vitro and in vivo. In vitro experiments demonstrated that PCL/LAP exhibited good cytocompatibility and enhanced the viability of BMSCs. PCL/LAP functioned to stimulate osteogenic differentiation of BMSCs at the mRNA and protein levels and elevated angiogenic gene expression and cytokine secretion. Moreover, BMSCs cultured on PCL/LAP promoted the angiogenesis potential of endothelial cells by angiogenic cytokine secretion. Then, PCL/LAP scaffolds were implanted into the calvarial defect model. Toxicological safety of PCL/LAP was confirmed, and significant enhancement of vascularized bone formation was observed. Taken together, 3D-printed PCL/LAP scaffolds with brilliant osteogenesis to enhance bone regeneration could be envisaged as an outstanding bone substitute for a promising change in oral-maxillofacial bone defect reconstruction.

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One-step fabrication of an organ-on-a-chip with spatial heterogeneity using a 3D bioprinting technology

Lab on a Chip ◽

10.1039/c6lc00450d ◽

2016 ◽

Vol 16 (14) ◽

pp. 2618-2625 ◽

Cited By ~ 133

Author(s):

Hyungseok Lee ◽

Dong-Woo Cho

Keyword(s):

3D Printing ◽

Spatial Heterogeneity ◽

3D Bioprinting ◽

Fabrication Method ◽

Microfluidic Systems ◽

Printing Technology ◽

3D Printing Technology ◽

One Step ◽

Organ On A Chip ◽

Spatial Cell

A one-step fabrication method using a 3D printing technology for whole organ-on-a-chip platforms, including microfluidic systems, which possess spatial cell/ECM heterogeneity.

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