Developing a Predictive Tool for Reactive Oxygen Species Damage During Bovine Sperm Storage at Ambient Temperature

Reactive oxygen species overgeneration as a side effect of semen cryopreservation may lead to lipid peroxidation, protein degradation, DNA fragmentation and cell death, resulting in a decrease of sperm survival and fertilisation ability. Lycopene has been proposed as a potential supplement to semen extenders because of its antioxidant properties. The aim of this study was to evaluate the effects of lycopene on the structural integrity, functional activity and selected oxidative stress parameters of cryopreserved bovine sperm. Thirty bovine ejaculates were split into two aliquots and diluted with a commercial semen extender supplemented with 1.5 mmol/l lycopene or containing no supplement (control), cooled down to 4 °C, frozen and kept in liquid nitrogen. Prior to experiments, frozen straws were thawed at 37 °C for 20 s. Lycopene addition resulted in a higher sperm motility (P < 0.001), progressive motility (P < 0.001) and all secondary motion characteristics (P < 0.001 with respect to the average path velocity, linear velocity, velocity of curvilinear motion, beat cross frequency, path straightness and linearity; P < 0.01 in the case of the amplitude of lateral head displacement). Furthermore, lycopene exhibited protective effects on the sperm membrane (P < 0.05) and acrosomal (P < 0.01) integrity in comparison to control. An assay for metabolic function revealed that lycopene supplementation to the cryopreservation medium resulted in a higher preservation of the sperm mitochondrial activity (P < 0.001). Reactive oxygen species production as well as intracellular superoxide generation were decreased following lycopene addition (P < 0.01 in the case of reactive oxygen species; P < 0.001 with respect to superoxide production). Finally, the presence of lycopene led to a decrease in protein carbonyl production (P < 0.01), lipid peroxidation (P < 0.001) as well as oxidative DNA damage (P < 0.05) when compared to control. In conclusion, lycopene exhibited significant reactive oxygen species-trapping and antioxidant properties which may prevent oxidative damage to frozen-thawed sperm, and, thus, enhance the post-thaw vitality of male reproductive cells in cattle breeding.

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Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase

Theriogenology ◽

10.1016/s0093-691x(01)00702-6 ◽

2002 ◽

Vol 57 (3) ◽

pp. 1105-1122 ◽

Cited By ~ 76

Author(s):

Jean-François Bilodeau ◽

Sophie Blanchette ◽

Nathaly Cormier ◽

Marc-André Sirard

Keyword(s):

Reactive Oxygen Species ◽

Sperm Motility ◽

Reactive Oxygen ◽

Bovine Liver ◽

Egg Yolk ◽

Oxygen Species ◽

Metal Chelators ◽

Bovine Sperm ◽

Oviductal Fluid

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Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm

Theriogenology ◽

10.1016/j.theriogenology.2016.02.007 ◽

2016 ◽

Vol 86 (2) ◽

pp. 562-571 ◽

Cited By ~ 53

Author(s):

H. Gürler ◽

E. Malama ◽

M. Heppelmann ◽

O. Calisici ◽

C. Leiding ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Dna Damage ◽

Reactive Oxygen ◽

Oxygen Species ◽

Sperm Viability ◽

Bovine Sperm

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Response of Posidonia oceanica (L.) Delile and its associated N2 fixers to different combinations of temperature and light levels

10.1101/2021.08.13.456246 ◽

2021 ◽

Author(s):

Manuela Gertrudis García-Márquez ◽

Víctor Fernández-Juárez ◽

José Carlos Rodríguez-Castañeda ◽

Nona S. R. Agawin

Keyword(s):

Reactive Oxygen Species ◽

Alkaline Phosphatase ◽

Primary Production ◽

Ambient Temperature ◽

Elevated Temperatures ◽

Reactive Oxygen ◽

Posidonia Oceanica ◽

Oxygen Species ◽

Seagrass Meadows ◽

Light Levels

Ocean warming and water turbidity are threats for the persistence of seagrass meadows and their effects on the productivity of seagrasses and the functioning of their associated microorganisms have not been studied extensively. The purpose of this study was to assess the effects of different light levels and temperatures on Posidonia oceanica, the endemic seagrass species in the Mediterranean Sea, and their N2 fixing community, which contributes importantly to the nitrogen requirements and high productivity of the plants. Aquarium experiments were conducted in winter when the plants are more vulnerable to changes in temperature, subjecting them to short-term exposures to ambient (15.5 °C) and elevated temperatures (ambient+5.5 °C) and at limited (13 μmol photons m-2 s-1) and saturating light conditions (124 μmol photons m-2 s-1). Primary production, chlorophyll content, reactive oxygen species production, polyphenols content, the nifH gene expression, N2 fixation and alkaline phosphatase activities were measured in different plant tissues. Plants incubated at ambient temperature and high light exhibited enhanced total chlorophyll production and significantly higher gross and net primary production, which were approximately two-fold compared to the rest of the treatments. The oxidative stress analyses revealed increased production of reactive oxygen species in young leaves incubated at ambient temperature and saturating light, while the polyphenols content in top leaves was considerably higher under elevated temperatures. In contrast, N2 fixation and alkaline phosphatase rates were significantly higher under elevated temperature and low light levels. The presence of the N2 fixing phylotypes UCYN-A, -B and -C was detected through genetic analyses, with UCYN-B demonstrating the highest nifH gene transcription levels at elevated temperatures. These findings emphasize the significant role of irradiance on the productivity of P. oceanica and the temperature dependence of the N2 fixation process in winter.

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Mitochondria as a source of reactive oxygen species

Biochemical Journal ◽

10.1042/bj20090056 ◽

2009 ◽

pp. c3 ◽

Cited By ~ 1

Author(s):

Helena M. Cochemé ◽

Michael P. Murphy

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Oxygen Species

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Clinical aspects of reactive oxygen and nitrogen species

Biochemical Society Symposium ◽

10.1042/bss0710121 ◽

2004 ◽

Vol 71 ◽

pp. 121-133 ◽

Cited By ~ 25

Author(s):

Ascan Warnholtz ◽

Maria Wendt ◽

Michael August ◽

Thomas Münzel

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Risk Factor ◽

Endothelial Dysfunction ◽

Vascular Tissue ◽

Rapid Development ◽

Reactive Oxygen ◽

Clinical Aspects ◽

No Production ◽

Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

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