Genetic Analysis of p17S-208 Plasmid Encoding the Colistin Resistance mcr-3 Gene in Escherichia coli Isolated from Swine in South Korea

2019 ◽  
Vol 25 (3) ◽  
pp. 457-461 ◽  
Author(s):  
Kuastros Mekonnen Belaynehe ◽  
Seung Won Shin ◽  
Kyung Yoon Park ◽  
Ji Young Jang ◽  
Ho Geun Won ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Analysis ◽  
South Korea ◽  
Colistin Resistance

scholarly journals First Report of an Escherichia coli Strain Carrying the Colistin Resistance Determinant mcr-1 from a Dog in South Korea

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 768
Author(s):  
Dong Chan Moon ◽  
Abraham Fikru Mechesso ◽  
Hee Young Kang ◽  
Su-Jeong Kim ◽  
Ji-Hyun Choi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South Korea ◽  
Companion Animals ◽  
Coli Strain ◽  
Colistin Resistance ◽  
First Report ◽  
E Coli ◽  
Risk Gene ◽  
Additional Resistance ◽  
Third Generation Cephalosporins

We studied the presence of the mobile colistin resistance gene mcr-1 in Escherichia coli isolates recovered from fecal and urine samples of companion animals, that were collected from South Korea in 2018 and 2019. The mcr-1 gene was detected in one colistin-resistant E. coli isolated from a diarrheic dog. The isolate exhibited additional resistance to multiple antimicrobials, including fluoroquinolones and third-generation cephalosporins. The mcr-1 carrying isolate belonged to ST160. The pulsed-field gel electrophoresis pattern of our strain differed from those ST160 E. coli strains previously identified from chickens in Korea. The mcr-1 gene was identified in the IncI2 plasmid. It was also transferred to E. coli J53 recipient strain, with a conjugation efficiency of 2.8 × 10−4. Average nucleotide identity analysis demonstrated that the mcr-1-carrying plasmid in this study was closely related to those from patients in Korea. To the best of our knowledge, this is the first report of mcr-1 carrying E. coli from a companion animal in South Korea. Our findings support One Health approach is necessary to prevent the dissemination of this high-risk gene.

scholarly journals Emergence of mcr-1 and mcr-3 variants coding for plasmid-mediated colistin resistance in Escherichia coli isolates from food- producing animals in South Korea

2018 ◽  
Vol 72 ◽  
pp. 22-24 ◽  
Author(s):  
Kuastros Mekonnen Belaynehe ◽  
Seung Won Shin ◽  
Kyung Yoon Park ◽  
Ji Young Jang ◽  
Ho Geun Won ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
South Korea ◽  
Colistin Resistance

scholarly journals Decreased colistin resistance and mcr-1 prevalence in pig-derived Escherichia coli in Japan after banning colistin as a feed additive

2021 ◽  
Vol 24 ◽  
pp. 383-386
Author(s):  
Masaru Usui ◽  
Yohei Nozawa ◽  
Akira Fukuda ◽  
Tomomi Sato ◽  
Michi Yamada ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Feed Additive ◽  
Colistin Resistance

scholarly journals Mobile Colistin Resistance Gene mcr-1 Detected on an IncI2 Plasmid in Salmonella Typhimurium Sequence Type 19 from a Healthy Pig in South Korea

2021 ◽  
Vol 9 (2) ◽  
pp. 398
Author(s):  
Dong Chan Moon ◽  
Su-Jeong Kim ◽  
Abraham Fikru Mechesso ◽  
Hee Young Kang ◽  
Hyun-Ju Song ◽  
...  
Keyword(s):  
South Korea ◽  
Salmonella Typhimurium ◽  
Bacterial Infections ◽  
Salmonella Enteritidis ◽  
Sequence Type ◽  
Intestinal Epithelial ◽  
Colistin Resistance ◽  
Salmonella Gallinarum ◽  
Additional Resistance ◽  
Food Animals

Colistin is considered the last resort for the treatment of multi-drug resistant Gram-negative bacterial infections. We studied colistin resistance and the mcr-1 gene carriage in Salmonella isolates recovered from food animals in South Korea between 2010 and 2018. Colistin resistance was found in 277 isolates, predominantly in Salmonella Enteritidis (57.1%) and Salmonella Gallinarum (41.9%). However, the mcr-1 gene was identified in only one colistin-resistant Salmonella Typhimurium (MIC = 16 µg/mL) isolated from a healthy pig. The mcr-1 carrying isolate presented additional resistance to multiple antimicrobials. The strain belonged to sequence type (ST)19 and carried various virulence factor genes that are associated with adhesion and invasion of Salmonella into intestinal epithelial cells, as well as its survival in macrophages. The mcr-1 gene was identified on an IncI2 plasmid and it was also transferred to the E. coli J53 recipient strain. The mcr-1-carrying plasmid (pK18JST013) in this study was closely related to that previously reported in S. Indiana (pCFSA664-3) from chicken in China. This is the first report of mcr-1 carrying S. Typhimurium in South Korea. The finding indicates the importance of regular screening for the presence of the mcr-1 gene in S. Typhimurium in food animals to prevent the spread to humans.

scholarly journals Genomic Characterization of New Variant of Hydrogen Sulfide (H2S)-Producing Escherichia coli with Multidrug Resistance Properties Carrying the mcr-1 Gene in China

Antibiotics ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 80 ◽  
Author(s):  
Silpak Biswas ◽  
Mohammed Elbediwi ◽  
Guimin Gu ◽  
Min Yue
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Sulfide ◽  
Multidrug Resistance ◽  
Bacterial Infections ◽  
Multidrug Resistant ◽  
Health Concern ◽  
Colistin Resistance ◽  
New Variant ◽  
Genomic Characterization ◽  
Human Infections

Colistin is considered to be a ‘last-resort’ antimicrobial for the treatment of multidrug-resistant Gram-negative bacterial infections. Identification of Enterobacteriaceae, carrying the transferable colistin resistance gene mcr-1, has recently provoked a global health concern. This report presents the first detection of a hydrogen sulfide (H2S)-producing Escherichia coli variant isolated from a human in China, with multidrug resistance (MDR) properties, including colistin resistance by the mcr-1 gene, which could have great implications for the treatment of human infections.

scholarly journals Emergence of Plasmid-Mediated Colistin Resistance Genemcr-1in a Clinical Escherichia coli Isolate from Egypt

2016 ◽  
Vol 60 (5) ◽  
pp. 3249-3250 ◽  
Author(s):  
Shimaa S. Elnahriry ◽  
Hazim O. Khalifa ◽  
Ahmed M. Soliman ◽  
Ashraf M. Ahmed ◽  
Alaaddin M. Hussein ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Colistin Resistance ◽  
Coli Isolate

scholarly journals Emergence of colistin resistance genes (mcr-1) in Escherichia coli among widely distributed wild ungulates

2021 ◽  
pp. 118136
Author(s):  
Rita Tinoco Torres ◽  
Mónica V. Cunha ◽  
Débora Araujo ◽  
Helena Ferreira ◽  
Carlos Fonseca ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Wild Ungulates ◽  
Colistin Resistance

Further genetic analysis of the C-terminal external loop region in Escherichia coli maltoporin

1997 ◽  
Vol 148 (5) ◽  
pp. 375-387 ◽  
Author(s):  
P.E. Klebba ◽  
S.M.C. Newton ◽  
A. Charbit ◽  
V. Michel ◽  
D. Perrin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Analysis ◽  
External Loop ◽  
Loop Region

scholarly journals Molecular survey of mcr1 and mcr2 plasmid mediated colistin resistance genes in Escherichia coli isolates of animal origin in Iran

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Kayhan Ilbeigi ◽  
Mahdi Askari Badouei ◽  
Hossein Vaezi ◽  
Hassan Zaheri ◽  
Sina Aghasharif ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Companion Animals ◽  
Multidrug Resistant ◽  
Animal Origin ◽  
Colistin Resistance ◽  
E Coli ◽  
High Level ◽  
Farming Industry

Abstract Objectives The emergence of colistin-resistant Enterobacteriaceae from human and animal sources is one of the major public health concerns as colistin is the last-resort antibiotic for treating infections caused by multidrug-resistant Gram-negative bacteria. We aimed to determine the prevalence of the prototype widespread colistin resistance genes (mcr-1 and mcr-2) among commensal and pathogenic Escherichia coli strains isolated from food-producing and companion animals in Iran. Results A total of 607 E. coli isolates which were previously collected from different animal sources between 2008 and 2016 used to uncover the possible presence of plasmid-mediated colistin resistance genes (mcr-1 and mcr-2) by PCR. Overall, our results could not confirm the presence of any mcr-1 or mcr-2 positive E. coli among the studied isolates. It is concluded that despite the important role of food-producing animals in transferring the antibiotic resistance, they were not the main source for carriage of mcr-1 and mcr-2 in Iran until 2016. This study suggests that the other mcr variants (mcr-3 to mcr-9) might be responsible for conferring colistin resistance in animal isolates in Iran. The possible linkage between pig farming industry and high level of mcr carriage in some countries needs to be clarified in future prospective studies.

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