Increased Proportion of Hematopoietic Stem and Progenitor Cell Population in Cord Blood of Neonates Born to Mothers with Gestational Diabetes Mellitus

Abstract Background and Purpose There are abundant hematopoietic stem cells (HSCs) in cord blood. It was known that HSCs continue to differentiate into common lymphoid progenitors (CLPs) and common myeloid progenitors (CMPs). Furthermore, the CMPs could differentiate into megakaryocyte erythroid progenitors (MEPs), and MEPs ultimately differentiated into platelets and erythrocytes. It had been reported that the proportion of HSCs in cord blood was higher than that in healthy pregnant women, so as the incidence of neonatal polycythemia in gestational diabetes mellitus (GDM) patients. We aimed to investigate the HSCs population and the change of their differentiation in the cord blood of neonates of GDM mothers. Methods In this study, we collected cord blood from GDM and healthy pregnant women at delivery. Totally 75 cases were included, in which 52 were for healthy control group and 23 were for GDM group. Then the number and differentiation status of HSCs in cord blood of the two groups were observed. HSC (CD45+ CD34+), CLP (CD45+ CD34+ CD7+) and CMP (CD45+ CD34+ CD33+) cells were identified and quantified by flow cytometry. Results Compared to healthy controls, HSC number in cord blood from GDM group were significantly increased (0.77±0.063% vs.1.28±0.176%，p=0.0113). The number of CMP cells which were differentiated from HSCs were higher than control (28.69±3.223% vs.46.43±4.927%, p=0.0033), while CLP cell number were lower (27.04±2.044% vs.21.11±2.406%, p=0.0475). Conclusion In conclusion, compared with non-diabetic pregnant women, there were more HSCs in the cord blood of the newborns of GDM patients, and the differentiation of HSCs to CMP cells was increased, while the differentiation to CLP cells was decreased. Those findings were probably caused by the high glucose microenvironment and insulin medication in pregnant women with GDM, and the HSCs differentiation changes might be influencing factors of the high incidence of neonatal erythrocytosis in GDM patients.

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Umbilical Cord Blood Acidosis in Term Pregnancies With Gestational Diabetes Mellitus and Its Relations to Maternal Factors and Neonatal Outcomes

Iranian Red Crescent Medical Journal ◽

10.5812/ircmj.59267 ◽

2018 ◽

Vol 20 (S1) ◽

Author(s):

Soroush Aalipour ◽

Sedigheh Hantoushzadeh ◽

Mamak Shariat ◽

Sadaf Sahraian ◽

Mahdi Sheikh

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Neonatal Outcomes ◽

Maternal Factors

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Gestational diabetes mellitus increases the baseline level of procalcitonin in maternal blood but not in umbilical cord blood in late pregnancy

Medicine ◽

10.1097/md.0000000000014914 ◽

2019 ◽

Vol 98 (11) ◽

pp. e14914

Author(s):

Yan Ma ◽

Wenfeng Ye ◽

Yanhong Tang

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Baseline Level ◽

Late Pregnancy ◽

Maternal Blood

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PP-308. Cord blood ferritin and serum transferrin receptor levels of infants born to mothers with gestational diabetes mellitus

Early Human Development ◽

10.1016/j.earlhumdev.2010.09.364 ◽

2010 ◽

Vol 86 ◽

pp. S139

Author(s):

Senay Coskun ◽

Hulya Bilgen ◽

Hulya Ozdemir ◽

Su Gulsun Berrak ◽

Sedef Bilgic ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Transferrin Receptor ◽

Serum Transferrin ◽

Serum Transferrin Receptor

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Serum Levels and Placental Expression of NGAL in Gestational Diabetes Mellitus

International Journal of Endocrinology ◽

10.1155/2020/8760563 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Xiaoqian Yin ◽

Yan Huo ◽

Li Liu ◽

Yixing Pan ◽

Suxin Liu ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Cord Blood ◽

Umbilical Cord ◽

Serum Levels ◽

Maternal Blood ◽

Maternal Serum ◽

Positive Correlation ◽

Protein Levels

Objectives. The aim was to investigate neutrophil gelatinase-associated lipocalin (NGAL) levels in the serum and term placentas and its potential role in gestational diabetes mellitus (GDM). Methods. A total of 49 GDM subjects and 39 age-matched women with normal pregnancies were recruited. We examined serum concentrations of NGAL and tumor necrosis factor-α (TNF-α) in maternal blood and cord blood and their expression levels in the term placentas and umbilical cord. Results. Serum NGAL levels were significantly higher in GDM patients than in normal pregnant controls both in the maternal blood (4.80 ± 1.99 vs. 3.66 ± 1.13, P=0.001) and the cord blood (4.70 ± 2.08 vs. 3.85 ± 1.44, P=0.027). Moreover, serum NGAL levels exhibited a positive correlation with various parameters of insulin resistance. Maternal serum NGAL levels positively correlated with the NGAL levels found in the cord blood of the control (r = 0.399, P=0.012) and the GDM subjects (r = 0.349, P=0.014). Finally, the expression of NGAL protein levels in the placenta (1.22 ± 0.39 vs. 0.65 ± 0.23, P<0.001) and umbilical cord (0.65 ± 0.23 vs. 0.25 ± 0.10, P<0.001) were higher in GDM women than those noted in the control subjects. In the GDM group, maternal serum NGAL levels exhibited a positive correlation with placental NGAL mRNA and protein levels (r = 0.848, P=0.008; r = 0.636, P=0.011, respectively). Conclusions. NGAL may be an important adipokine involved in GDM and fetal development. The oversecretion of NGAL from the placenta may contribute to the elevated levels of serum NGAL in gestational diabetes mellitus.

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