Phenotypic Fitness of Primary Endothelial Cells Cultured from Patients with High Cardiovascular Risk or Chronic Kidney Disease for Vascular Tissue Engineering

2014 ◽  
Vol 20 (5-6) ◽  
pp. 1049-1059
Author(s):  
Irma L. Geenen ◽  
Sanne Verbruggen ◽  
Daniel G. Molin ◽  
Henri M. Spronk ◽  
Jos G. Maessen ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Tissue Engineering ◽  
Endothelial Cells ◽  
Cardiovascular Risk ◽  
Kidney Disease ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
High Cardiovascular Risk ◽  
Phenotypic Fitness ◽  
Cells Cultured

scholarly journals Chronic kidney disease and bleeding risk in patients at high cardiovascular risk: a cohort study

2017 ◽  
Vol 16 (1) ◽  
pp. 65-73 ◽  
Author(s):  
G. Ocak ◽  
M. B. Rookmaaker ◽  
A. Algra ◽  
G. J. de Borst ◽  
P. A. Doevendans ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Cohort Study ◽  
Cardiovascular Risk ◽  
Kidney Disease ◽  
Bleeding Risk ◽  
High Cardiovascular Risk

Endothelial cells (ECs) for vascular tissue engineering: venous ECs are less thrombogenic than arterial ECs

2012 ◽  
Vol 9 (5) ◽  
pp. 564-576 ◽  
Author(s):  
I. L. A. Geenen ◽  
D. G. M. Molin ◽  
N. M. S. van den Akker ◽  
F. Jeukens ◽  
H. M. Spronk ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering

scholarly journals Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jeremy A. Antonyshyn ◽  
Vienna Mazzoli ◽  
Meghan J. McFadden ◽  
Anthony O. Gramolini ◽  
Stefan O. P. Hofer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Reliable Method ◽  
Vascular Tissue ◽  
Human Adipose Tissue ◽  
Vascular Tissue Engineering ◽  
Microvascular Endothelial Cells ◽  
Specific Uptake

AbstractEndothelial cells are among the fundamental building blocks for vascular tissue engineering. However, a clinically viable source of endothelium has continued to elude the field. Here, we demonstrate the feasibility of sourcing autologous endothelium from human fat – an abundant and uniquely dispensable tissue that can be readily harvested with minimally invasive procedures. We investigate the challenges underlying the overgrowth of human adipose tissue-derived microvascular endothelial cells by stromal cells to facilitate the development of a reliable method for their acquisition. Magnet-assisted cell sorting strategies are established to mitigate the non-specific uptake of immunomagnetic microparticles, enabling the enrichment of endothelial cells to purities that prevent their overgrowth by stromal cells. This work delineates a reliable method for acquiring human adipose tissue-derived microvascular endothelial cells in large quantities with high purities that can be readily applied in future vascular tissue engineering applications.

Identification and Reduction of Cryoinjury in Endothelial Cells: A First Step toward Establishing a Cell Bank for Vascular Tissue Engineering

2006 ◽  
Vol 0 (0) ◽  
pp. 061114080139001
Author(s):  
Karla Lehle ◽  
Markus Hoenicka ◽  
Volker R. Jacobs ◽  
Franz X. Schmid ◽  
Dietrich E. Birnbaum
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Cell Bank ◽  
A Cell

scholarly journals Is Chronic Kidney Disease Associated with a High Ankle Brachial Index in Adults at High Cardiovascular Risk?

2011 ◽  
Vol 18 (3) ◽  
pp. 224-230 ◽  
Author(s):  
Hao Liu ◽  
Hong Shi ◽  
Jinming Yu ◽  
Fang Chen ◽  
Qingwu Jiang ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Cardiovascular Risk ◽  
Kidney Disease ◽  
Ankle Brachial Index ◽  
High Cardiovascular Risk
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