Very low ileal nitrogen and amino acid digestibility of zein compared to whey protein isolate in healthy volunteers

2020 ◽  
Vol 113 (1) ◽  
pp. 70-82 ◽  
Author(s):  
Juliane Calvez ◽  
Simon Benoit ◽  
Julien Piedcoq ◽  
Nadezda Khodorova ◽  
Dalila Azzout-Marniche ◽  
...  
Keyword(s):  
Amino Acid ◽  
Healthy Volunteers ◽  
Whey Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Sampling Device ◽  
Ileal Digestibility ◽  
Healthy Humans ◽  
Single Meal ◽  
Plasma Leucine

ABSTRACT Background Whey protein and zein are of nutritional interest due to their high leucine content, but little data are available on their amino acid (AA) ileal digestibility. Objective This study aimed to determine ileal digestibility of whey protein isolate (WPI) and zein in healthy volunteers by use of the naso-ileal intubation method, which allows continuous collection of postprandial ileal digesta. Methods Twenty-two healthy volunteers were intubated with a naso-ileal sampling device positioned at the terminal ileum level. They received a single meal of protein-free biscuits and a drink containing zein (n = 8), WPI (n = 7), or no protein (protein free, n = 7). Ileal effluents and plasma samples were collected over a 9-h postprandial period. Total nitrogen and AA contents were quantified in effluents. True ileal digestibility was calculated after correction for endogenous losses evaluated in the protein-free group. Results True ileal nitrogen digestibility of zein was markedly lower than WPI (60.2 ± 4.5% and 91.2 ± 2.6%, respectively, P = 0.0003). True ileal digestibility of AAs ranged from 87.4 ± 2.7% for threonine to 98.4 ± 1.0% for methionine in the WPI group, and from 59.3 ± 5.6% for methionine to 69.0 ± 5.8% for arginine in the zein group. The digestible indispensable AA (IAA) score was 1.03 (histidine) for WPI and close to 0 for zein, owing to its negligible lysine content. Plasma IAA concentration significantly increased after WPI intake (P = 0.0319), whereas no effect of zein on aminoacidemia was observed, including plasma leucine, despite its high leucine content. Conclusions Our findings provide data on ileal digestibility of WPI and zein AAs in healthy humans and, in contrast to WPI, zein is poorly digestible. This study was registered at clinicaltrials.gov as NCT03279211.

scholarly journals True Ileal Protein Digestibility of Zein and Whey Protein Isolate in Healthy Humans (OR27-06-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Juliane Calvez ◽  
Simon Benoit ◽  
Léa Fleury ◽  
Nadezda Khodorova ◽  
Julien Piedcoq ◽  
...  
Keyword(s):  
Whey Protein ◽  
Flow Rate ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Nitrogen Flow ◽  
Nitrogen Flow Rate ◽  
Ileal Digestibility ◽  
Protein Sources ◽  
Dietary Nitrogen ◽  
True Digestibility

Abstract Objectives There are limited data available on true ileal nitrogen and amino acid digestibility of protein sources. The goal of this study was thus to determine for the first time in healthy volunteers the ileal digestibility of zein and whey proteins. Methods Twenty-two volunteers (10 women and 12 men; aged 37 ± 12y) were equipped with a double lumen intestinal tube positioned at the ileal level. They received a single meal of protein-free biscuits and a drink containing zein (n = 8), whey protein isolate (WPI, n = 7) or no protein (protein-free, n = 7). 13C-inuline was added to the drink as a non-absorbable marker. Ileal effluents were collected over a 9-h period after meal ingestion. Total nitrogen content was measured in effluents and correction for endogenous losses evaluated with the protein-free group was used to determine true digestibility of zein and WPI proteins. Results The mean ileal endogenous nitrogen flow was 3.89 ± 1.41mmol/h (mean ± SD). For zein, mean dietary nitrogen flow rate was 12.1 ± 6.9mmol/h, reaching 23.6 ± 13.5mmol/h 4h after the meal. In comparison, mean dietary nitrogen flow rate for WPI was significantly lower (1.6 ± 1.2mmol/h, P < 0.0001). Ileal apparent and true nitrogen digestibility of zein was markedly lower than WPI (11.7 ± 11.4% and 63.9 ± 5.9% for apparent digestibility of zein and WPI respectively, P < 0.0001; 32.2 ± 11.0% and 89.0 ± 5.8% for true digestibility of zein and WPI respectively, P < 0.0001). Conclusions In conclusion, we showed that zein is a poorly digestible protein. Among all the dietary proteins evaluated in humans, it presents the lowest value. Such a poor digestibility might be explained by the very low solubility of zein. On the contrary, the ileal digestibility of WPI is relatively high but lower than total milk proteins or casein measured in humans (around 95%). To further examine the digestibility of these 2 protein sources, amino acids bioavailability will be next evaluated. Funding Sources This study is funded by the Global Dairy Platform Incorporated. Supporting Tables, Images and/or Graphs

scholarly journals Postprandial Plasma Amino Acid Responses Between Standard Whey Protein Isolate and Whey Protein Isolate Plus Novel Technology

2019 ◽  
Vol 12 ◽  
pp. 117863881982797 ◽  
Author(s):  
Matthew H Sharp ◽  
Matthew W Stefan ◽  
Ryan P Lowery ◽  
Jacob M Wilson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Whey Protein ◽  
Plasma Levels ◽  
Muscle Protein ◽  
Plasma Concentrations ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Plasma Amino Acid ◽  
Whole Body

Background: Muscle mass is an important determinant of metabolic health and physical function. It has previously been demonstrated that the postprandial rise in circulating essential amino acids (EAA) acts as the main stimulus for muscle protein synthesis (MPS). This study investigated postprandial plasma amino acid (AA) responses of 2 different forms of whey protein isolate (WPI) with iso-caloric and iso-nitrogenous profiles to investigate plasma concentrations of EAA. Methods: In all, 12 healthy men (n = 12) between 19 and 32 years of age were recruited for a randomized, cross-over design, which involved consumption of protein supplements on 2 testing days separated by a 6-day washout period between conditions. On each testing day, subjects consumed either 29.6 g of WPI or WPI + io (whey protein isolate plus Ingredient Optimized Protein®) mixed with 236 mL of water. Plasma EAA and branch chain amino acid (BCAA) concentrations were assessed from whole body donated by subjects at pre-consumption and 30, 60, 90, 120, and 180 minutes post consumption. Results: Plasma levels of total EAA concentration was significantly greater in WPI + io at 30, 60, 90, and 120 minutes post consumption ( P < .01, P < .001, P < .01, and P < .01, respectively). Plasma levels of total BCAA concentration was significantly greater in WPI + io at 30, 60, 90, and 120 minutes post consumption ( P < .01, P < .001, P < .01, and P < .05, respectively) compared with WPI. For leucine, only WPI + io had elevated levels compared with pre-test at 90 minutes post consumption ( P < .001). Discussion: Both conditions significantly elevated EAA, BCAA, and leucine from basal levels. However, we conclude that the consumption of the treated WPI significantly raises plasma EAA, BCAA, and leucine to a greater extent compared with WPI with no treatment. Thus, supplementation with WPI that has undergone Ingredient Optimized® technology may be highly beneficial for those who partake in regular exercise, elderly individuals, or those affected by a reduced sensitivity to amino acids.

scholarly journals Real Ileal Digestibility of Sunflower Protein and Amino Acids in Humans

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 667-667
Author(s):  
Romain Tessier ◽  
Juliane Calvez ◽  
Nadezda Khodorova ◽  
Alain Quinsac ◽  
Romain Kapel ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Isolate ◽  
National Agency ◽  
Ileal Digestibility ◽  
Dietary Nitrogen ◽  
Healthy Humans ◽  
Indispensable Amino Acid ◽  
15N Enrichment ◽  
Sunflower Protein ◽  
Exogenous Nitrogen

Abstract Objectives Sunflower protein has a relatively well-balanced amino acid profile but it is little used in human food. Nutritional quality of sunflower protein isolate has never been directly measured in humans. This study aims to determine the ileal digestibility of protein and amino acid of a sunflower isolate incorporated in biscuits, in healthy humans. Methods Healthy volunteers (n = 7) were intubated with a naso-ileal tube. They ingested every 30 min for 4 h, sunflower biscuits for a total of 156 g of biscuits and 25 g of 15N intrinsically labeled sunflower protein isolate. A non-absorbable marker was perfused into the ileum. Ileal samples were continuously collected during 8 h after the first meal. Blood was sampled every 30 min for 4 h and every hour after and total urine was collected every 2 h. To measure nitrogen ileal digestibility, the percentage of nitrogen and 15N enrichment in ileal contents were assessed by an elemental analyzer coupled with and isotope ratio mass spectrometer (EA-IRMS). Indispensable amino acid (IAA) ileal digestibility was determined by measuring amino acid 15N enrichment by GC-C-IRMS and their quantification by UHPLC. Metabolic losses of dietary nitrogen were assessed by measuring 15N in urine and plasma. The digestible indispensable amino acid score (DIAAS) and the net post-prandial utilization (NPPU) were calculated. Results Exogenous nitrogen appeared in the ileum 1 h after the first meal, its flow was stable between 4 h and 7 h. Cumulated dietary nitrogen in the ileum was 14.0 ± 4.0% over 8 h. Sunflower nitrogen real ileal digestibility was 86.0 ± 4.0% of ingested nitrogen. Mean IAA ileal digestibility was 87.0 ± 6.1% with values ranging from 84.4% for lysine and threonine to 89.3 ± 7.8% for methionine. DIAAS was 0.93 for lysine. After 8 h, 4.0 ± 1.0% of dietary nitrogen was incorporated in plasma protein. Cumulated exogenous nitrogen in urinary urea was 4.0 ± 1.4% and exogenous nitrogen in body urea after 8 h was 9.8 ± 2.5%. The NPPU was 72.2 ± 3.7% at 8 h. Conclusions Sunflower isolate ileal digestibility is relatively low compared to other protein isolate assessed in similar conditions (ileal tube and 15N labeling of protein). Despite a moderate deficiency in lysine, sunflower protein isolate has a good post-prandial metabolic utilization. Funding Sources French Research National Agency (ANR), financial support of SOFIPROTEOL under the FASO Project PRODIA.

scholarly journals Real ileal digestibility of sunflower protein and amino acids in humans

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Romain Tessier ◽  
Juliane Calvez ◽  
Nadezda Khodorova ◽  
Alain Quinsac ◽  
Romain Kapel ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Healthy Volunteers ◽  
Protein Isolate ◽  
Nitrogen Flow ◽  
Dual Isotope ◽  
Ileal Digestibility ◽  
Isotope Method ◽  
Indispensable Amino Acids ◽  
Sunflower Protein

AbstractIntroduction:Sunflower protein is not used in human nutrition despite a relatively good amino acid composition. However, the bioavailability of sunflower isolate has never been measured in Humans. The goal of this work was to determine ileal digestibility of protein and amino acids from a sunflower isolate in healthy volunteers and to challenge newly developed dual isotope method.Materials and methods:Eight healthy volunteers were equipped with a naso-ileal tube. They received during four hours twelve doses of biscuits containing, in total, 25 g of 15N sunflower protein isolate together with 400 mg of a mixture of free 13C amino acids incorporated in chocolates. Polyethylene glycol was perfused as non-absorbable marker and ileal contents were collected during 8 hours after ingestion of the first meal. Real ileal digestibility was measured by assessing nitrogen and carbon content as well as 15N and 13C enrichments by EA-IRMS. Amino acid digestibility was determined by measuring 15N and 13C enrichments by GC-C-IRMS and quantity of amino acids by UPLC. Blood was collected for 8 h to determine 15N and 13C enrichments by GC-C-IRMS.Results:The ileal nitrogen flow was 2.7 ± 0.5 mL/min (mean ± SD). In average, 53.1 ± 12.0 mmol of exogenous nitrogen was recovered during the eight hours of experiment, resulting in an ileal digestibility of sunflower isolate was 85.6 ± 2.6 % of nitrogen ingested. 13C amino acids were also recovered at the ileal level. The mixture of free 13C revealed an ileal digestibility of 94.9 ± 0.9 %. Ileal indispensable amino acids digestibility and DIAAS are in progress.Discussion:Ileal digestibility of sunflower isolate incorporated in toasted biscuits was lower than the value found or a raw isolate in a rat model (94.5%). The study revealed that 5 % of free amino acids were not absorbed in the ileum. Amino acid digestibility will complete the study to evaluate the DIAAS of sunflower isolate and to compare values obtained with the standard method and with the dual isotope method.

scholarly journals Fabrication of Oil-in-Water Emulsions with Whey Protein Isolate–Puerarin Composites: Environmental Stability and Interfacial Behavior

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 705
Author(s):  
Yejun Zhong ◽  
Jincheng Zhao ◽  
Taotao Dai ◽  
Jiangping Ye ◽  
Jianyong Wu ◽  
...  
Keyword(s):  
Interfacial Tension ◽  
Whey Protein ◽  
Surface Protein ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Environmental Stability ◽  
Dependent Manner ◽  
Emulsifying Properties ◽  
Interfacial Behavior ◽  
Concentration Dependent Manner

Protein–polyphenol interactions influence emulsifying properties in both directions. Puerarin (PUE) is an isoflavone that can promote the formation of heat-set gels with whey protein isolate (WPI) through hydrogen bonding. We examined whether PUE improves the emulsifying properties of WPI and the stabilities of the emulsions. We found that forming composites with PUE improves the emulsifying properties of WPI in a concentration-dependent manner. The optimal concentration is 0.5%, which is the highest PUE concentration that can be solubilized in water. The PUE not only decreased the droplet size of the emulsions, but also increased the surface charge by forming composites with the WPI. A 21 day storage test also showed that the maximum PUE concentration improved the emulsion stability the most. A PUE concentration of 0.5% improved the stability of the WPI emulsions against environmental stress, especially thermal treatment. Surface protein loads indicated more protein was adsorbed to the oil droplets, resulting in less interfacial WPI concentration due to an increase in specific surface areas. The use of PUE also decreased the interfacial tension of WPI at the oil–water interface. To conclude, PUE improves the emulsifying activity, storage, and environmental stability of WPI emulsions. This result might be related to the decreased interfacial tension of WPI–PUE composites.

scholarly journals Whey Protein Isolate Microgel Properties Tuned by Crosslinking with Organic Acids to Achieve Stabilization of Pickering Emulsions

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1296
Author(s):  
Jéssica Thaís do Prado Silva ◽  
João Vitor Munari Benetti ◽  
Taís Téo de Barros Alexandrino ◽  
Odilio Benedito Garrido Assis ◽  
Jolet de Ruiter ◽  
...  
Keyword(s):  
Contact Angle ◽  
Organic Acids ◽  
Whey Protein ◽  
Physical Stability ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Pickering Emulsions ◽  
Food Grade ◽  
Oil Water ◽  
Coffee Oil

Whey protein isolate (WPI) can be used effectively to produce food-grade particles for stabilizing Pickering emulsions. In the present study, crosslinking of WPI microgels using organic acids (tannic and citric acids) is proposed to improve their functionality in emulsions containing roasted coffee oil. It was demonstrated that crosslinking of WPI by organic acids reduces the microgels’ size from ≈1850 nm to 185 nm and increases their contact angle compared to conventional WPI microgels, achieving values as high as 60°. This led to the higher physical stability of Pickering emulsions: the higher contact angle and smaller particle size of acid-crosslinked microgels contribute to the formation of a thinner layer of particles on the oil/water (O/W) interface that is located mostly in the water phase, thus forming an effective barrier against droplet coalescence. Particularly, emulsions stabilized by tannic acid-crosslinked WPI microgels presented neither creaming nor sedimentation up to 7 days of storage. The present work demonstrates that the functionality of these crosslinked WPI microgels can be tweaked considerably, which is an asset compared to other food-grade particles that mostly need to be used as such to comply with the clean-label policy. In addition, the applications of these particles for an emulsion are much more diverse as of the starting material.

Novel Whey Protein Isolate/Polyvinyl Biocomposite for Packaging: Improvement of Mechanical and Water Barrier Properties by Incorporation of Nano-silica

2021 ◽  
Author(s):  
Bruna Rage Baldone Lara ◽  
Paulo Sérgio de Andrade ◽  
Mario Guimarães Junior ◽  
Marali Vilela Dias ◽  
Lizzy Ayra Pereira Alcântara
Keyword(s):  
Whey Protein ◽  
Barrier Properties ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Nano Silica ◽  
Water Barrier
Sign in / Sign up

Export Citation Format

Share Document