scholarly journals Routine Hematoxylin and Eosin Stain is Sensitive and Specific for the Diagnosis of Cytomegalovirus (CMV) Infection in Gastrointestinal Biopsy Specimens

2013 ◽  
Vol 140 (suppl 1) ◽  
pp. A021-A021
Author(s):  
Shadi Qasem ◽  
Ling Guo ◽  
Ziyan Salih
Keyword(s):  
Cmv Infection ◽  
Biopsy Specimens ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Routine Hematoxylin and Eosin Stain Is Specific for the Diagnosis of Cytomegalovirus Infection in Gastrointestinal Biopsy Specimens

2018 ◽  
Vol 26 (6) ◽  
pp. 500-506 ◽  
Author(s):  
Ling Guo ◽  
Tom C. DeRoche ◽  
Ziyan T. Salih ◽  
Shadi A. Qasem
Keyword(s):  
Positive Predictive Value ◽  
Predictive Value ◽  
Cytomegalovirus Infection ◽  
High Specificity ◽  
Immunocompromised Patients ◽  
Cmv Infection ◽  
Biopsy Specimens ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Positive Results

Background. Gastrointestinal cytomegalovirus (CMV) infection is a serious complication in immunocompromised patients; clinicians often expect expedited results for biopsy specimens. Our goal is to determine the accuracy of identification of CMV on hematoxylin and eosin (H&E) stain. Methods and Results. A total of 361 biopsy specimens from 273 patients with suspicion for CMV infection were retrieved. CMV was detected by immunohistochemistry (IHC) in 37 specimens acquired from 33 individual patients (average age = 54 years). Among the CMV-positive patients, 29 (88%) were reported to be immunosuppressed. Colon was the most common affected location. Of 37 CMV-positive specimens by IHC, 28 were positive by H&E (76%), 6 were negative (16%), and 3 were suspicious (8%). Of the 29 positive specimens on H&E, 28 were confirmed by IHC (97%) and 1 was indeterminate (3%). The sensitivity and specificity of H&E were 84% and 94%, respectively; the positive predictive value was 97%, and the negative predictive value was 93% ( P < .00001). Conclusion. Our results show that a preliminary diagnosis of CMV infection, based on H&E stains, can be reported with high specificity and low risk for false-positive results. Suspicious cases should be deferred pending the result of IHC stains.

Cytomegalovirus infection in the patients with inflammatory bowel disease

2013 ◽  
Vol 4 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Darya Olegovna Markova ◽  
Ruslan Abdullayevich Nasyrov ◽  
Mariya Olegovna Revnova
Keyword(s):  
Inclusion Bodies ◽  
Cytomegalovirus Infection ◽  
Immunosuppressive Drugs ◽  
Treatment Resistant ◽  
Cmv Infection ◽  
Resistant Disease ◽  
Biopsy Specimens ◽  
Colon Biopsy ◽  
Inflammatory Bowel ◽  
Hematoxylin And Eosin

Cytomegalovirus infection of the gastrointestinal tract is a relatively common occurrence in patients receiving immunosuppressive drugs, those infected with HIV, or those who are transplantant recepients. CMV infection may be diagnosed if typical intranuclear inclusion bodies are seen on standard hematoxylin and eosin (H&E) stain. However, the sensitivity of H&E exam for CMV infection is low. Immunohistochemistry (IHC) exam performed on colon biopsy specimens is more sensitive than routine histologic exam and has excellent specificity. Currently IHC is considered the gold standard for detecting CMV CMV infection in patients with refractory or complicated IBD should be ruled out before aggressive immunosuppressive therapy for treatment-resistant disease to decrease morbidity and mortality.

scholarly journals Histological Remission in Ulcerative Colitis in Deep Remission under Treatment with Adalimumab

2021 ◽  
Vol 30 (1) ◽  
pp. 55-58
Author(s):  
Antonio Tursi ◽  
Rosanna Nenna ◽  
Giovanni Musci ◽  
Walter Elisei ◽  
Marcello Picchio
Keyword(s):  
Ulcerative Colitis ◽  
Biopsy Specimens ◽  
Histological Activity ◽  
Hematoxylin And Eosin ◽  
The Mean ◽  
Histological Remission ◽  
Four Quadrant ◽  
Hematoxylin And Eosin Stain ◽  
First Time

Background and Aims: Histological remission (HR) has been recently demonstrated as the last therapeutic goal in ulcerative colitis (UC), but it is unknown whether and how it may occur. Our aim was to assess the histology during the follow-up of an UC population in deep remission under treatment with adalimumab (ADA). Methods: We performed a retrospective study on 22 UC patients who were in deep remission and followed-up while receiving therapy with ADA. Colonoscopy in those patients was performed every year. Four-quadrant biopsies every 10 cm were obtained during each colonoscopy and assessed by hematoxylin and eosin stain. Histological activity was classified using the Geboes scale. Results: A total of 22 patients were enrolled in the study. The mean follow-up of those patients was 28±7 months, and 2,592 biopsy specimens in total were taken during 108 colonoscopies performed during the follow-up. At the beginning of the follow-up, histological inflammation was found in 15/22 (68.2%) of patients in deep remissio while receiving maintenance ADA therapy, 8/22 (36.4%) of them with Geboes score ≥3.1. At the end of the follow-up, when patients were still in deep remission while receiving maintenance ADA therapy, only 4 patients (18.2%) had at least one biopsy specimen with evidence of any histological inflammation during the follow-up; only two patients (9.1%) had Geboes score ≥3.1. Conclusions: Our study shows for the first time that UC patients in deep remission under ADA may reach HR, but it seems slower than other clinical or endoscopic goals.

Histologic Evaluation of Osseous Regeneration Following Combination Therapy With Platelet-Rich Plasma and Bio-Oss in a Rat Calvarial Critical-Size Defect Model

2015 ◽  
Vol 41 (5) ◽  
pp. 543-549 ◽  
Author(s):  
Philip J. DeNicolo ◽  
M. Kelly Guyton ◽  
Michael F. Cuenin ◽  
Steven D. Hokett ◽  
Mohamed Sharawy ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Growth ◽  
Critical Size ◽  
Close Contact ◽  
Platelet Rich Plasma ◽  
Critical Size Defect ◽  
Histologic Evaluation ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Osseous Regeneration

Platelet-rich plasma (PRP) is an autogenous source of growth factors shown to facilitate human bone growth. Bio-Oss, an osteoconductive xenograft, is used clinically to regenerate periodontal defects, restore dental alveolar ridges, and facilitate sinus-lift procedures. The purpose of this study was to analyze whether a combination of PRP and Bio-Oss would enhance bone regeneration better than either material alone. PRP and/or Bio-Oss were administered in an 8-mm critical-size defect (CSD) rat calvarial model of bone defect between 2 polytetrafluoroethylene membranes to prevent soft tissue incursion. Eight weeks after the induction of the CSD, histologic sections were stained with hematoxylin and eosin stain and analyzed via light microscopy. Qualitative analyses revealed new bone regeneration in all 4 groups. The Bio-Oss and PRP plus Bio-Oss groups demonstrated greater areas of closure in the defects than the control or PRP-only groups because of the space-maintaining ability of Bio-Oss. The groups grafted with Bio-Oss showed close contact with new bone growth throughout the defects, suggesting a stronger graft. The use of PRP alone or in combination with Bio-Oss, however, did not appear to enhance osseous regeneration at 8 weeks. Areas grafted with Bio-Oss demonstrated greater space-maintaining capacity than controls, and PRP was an effective vehicle for placement of the Bio-Oss. However, at 8 weeks this study was unable to demonstrate a significant advantage of using PRP plus Bio-Oss over using Bio-Oss alone.

scholarly journals Role of S-100 Immunostain as An Auxiliary Diagnostic Aid in Leprosy

2017 ◽  
Vol 56 (205) ◽  
pp. 141-144 ◽  
Author(s):  
Ramesh Dhakhwa ◽  
Sneh Acharya ◽  
Sailesh Pradhan ◽  
Sanju Babu Shrestha ◽  
Tomoo Itoh
Keyword(s):  
Erythema Nodosum ◽  
Lupus Vulgaris ◽  
Common Pattern ◽  
Tissue Sections ◽  
Histopathologic Diagnosis ◽  
S 100 ◽  
Skin Biopsies ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Introduction: Histopathologic diagnosis of leprosy is difficult when Bacillary Index (BI) is zero and neural involvement are not easily identifiable on routine Hematoxylin and Eosin stain. This study was undertaken to study the role of S-100 immunostaining in demonstrating different patterns of nerve involvement in various types of leprosy. Methods: Thirty one skin biopsies with clinico-histopathologic diagnoses of leprosy over a period of two years were included in the study. Ten cases of non-lepromatous granulomatous dermatoses (including eight cases of lupus vulgaris and two cases of erythema nodosum) were used as controls. Tissue sections from all cases and controls were stained with Hematoxylin and Eosin (H&E) stain, Fite stain and S-100 immunostain. The H&E stained slides were used to study the histopathological features, Fite stained slides for Bacillary Index and S-100 for nerve changes. Results: Neural changes could be demonstrated in the entire spectrum of leprosy using S-100 immunostaining. The most common pattern of nerve destruction in the tuberculoid spectrum was fragmented and infiltrated whereas lepromatous spectrum showed mostly fragmented nerve twigs. Intact nerves were not detected in any of the leprosy cases. Conclusions:  S-100 immunostain is a useful auxiliary aid to the routine  H&E stain in the diagnosis of leprosy especially tuberculoid spectrum and intermediate leprosy.  Keywords: bacillary index; leprosy; S-100 immunostain.

scholarly journals Mucormycosis of the hard palate masquerading as carcinoma

2012 ◽  
Vol 2 (1) ◽  
pp. 28 ◽  
Author(s):  
Bhari Sharanesha Manjunatha ◽  
Nagarajappa Das ◽  
Rakesh V. Sutariya ◽  
Tanveer Ahmed
Keyword(s):  
Clinical Presentation ◽  
Fungal Infections ◽  
Survival Rates ◽  
Positive Culture ◽  
Staining Technique ◽  
Necrotic Bone ◽  
Chronic Ulcers ◽  
Hematoxylin And Eosin ◽  
Clinical Differential Diagnosis ◽  
Hematoxylin And Eosin Stain

A growing number of medically compromised patients are encountered by dentists in their practices. Opportunistic fungal infections such as mucormycosis usually occur in immunocompromised patients but can infect healthy individuals as well. Mucormycosis is an acute opportunistic, uncommon, frequently fatal fungal infection, caused by a saprophytic fungus that belongs to the class of phycomycetes. Among the clinical differential diagnosis we can consider squamous cell carcinoma. Such cases present as chronic ulcers with raised margins causing exposure of underlying bone. There is a close histopathological resemblance between mucormycosis and aspergillosis. Microscopically, aspergillosis has septate branching hyphae, which can be distinguished from mucormycotic hyphae by a smaller width and prominent acute angulations of branching hyphae. A definitive diagnosis of mucormycosis can be made by tissue biopsy that identifies the characteristic hyphae, by positive culture or both. The culture of diseased tissue may be negative and histopathologic examination is essential for early diagnosis. Mucormycosis was long regarded as a fatal infection with poor prognosis. However with early medical and surgical management survival rates are now thought to exceed 80%. In the present case, the fungus was identified by hematoxylin and eosin stain and confirmed by Grocott’s silver methenamine special staining technique. Removal of the necrotic bone, which acted as a nidus of infection, was done. Post-operatively patient was advised an obturator to prevent oronasal regurgitation. Since mucormycosis occurs infrequently, it may pose a diagnostic and therapeutic dilemma for those who are not familiar with its clinical presentation.

Crooked Calf Disease: A Histological and Histochemical Examination of Eight Affected Calves

1986 ◽  
Vol 23 (6) ◽  
pp. 734-740 ◽  
Author(s):  
L. C. Abbott ◽  
R. H. Finnell ◽  
G. F. Chernoff ◽  
S. M. Parish ◽  
C. C. Gay
Keyword(s):  
Congenital Anomalies ◽  
Adenosine Triphosphatase ◽  
Normal Muscle ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Histochemical Examination

Macroscopic, histopathologic, and histochemical investigations were made on a group of eight neonatal Angus × Hereford calves, selected from an ongoing outbreak of crooked calf disease among calving heifers. Arthrogryposis of the forelimbs was seen to varying degrees in all eight animals, and torticollis was present in six calves. Histopathology, using hematoxylin and eosin stain, did not reveal any striking or consistent lesion in the affected animals; the majority of the tissues sampled were normal. Muscle samples were processed for adenosine triphosphatase (ATPase) and NADH-tetrazolium reductase (NADH-tr) histochemistry, and the data suggest that a primary myopathy is not responsible for the congenital anomalies in the affected calves.

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