The Fibrometer System for Routine Coagulation Tests: Prothrombin Time and Partial Thromboplastin Time, Macro and Micro

1965 ◽  
Vol 43 (5) ◽  
pp. 475-480 ◽  
Author(s):  
John B. Miale ◽  
A. Ruth Winningham ◽  
Jessie W. Kent
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Coagulation Tests ◽  
Routine Coagulation

Can RoTEM®analysis be applied for haemostatic monitoring in paediatric congenital heart surgery?

2011 ◽  
Vol 21 (6) ◽  
pp. 684-691 ◽  
Author(s):  
Jo Bønding Andreasen ◽  
Anne-Mette Hvas ◽  
Kirsten Christiansen ◽  
Hanne Berg Ravn
Keyword(s):  
Cardiac Surgery ◽  
Platelet Count ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Congenital Heart ◽  
Heart Surgery ◽  
Congenital Heart Surgery ◽  
Activated Partial Thromboplastin Time ◽  
Clotting Time ◽  
Coagulation Tests

AbstractBackgroundSuccessful management of bleeding disorders after congenital heart surgery requires detection of specific coagulation disturbances. Whole-blood rotation thromboelastometry (RoTEM®) provides continuous qualitative haemostatic profiles, and the technique has shown promising results in adult cardiac surgery.SettingTo compare the performance of RoTEM®with that of conventional coagulation tests in children, we conducted a descriptive study in children undergoing congenital cardiac surgery. For that purpose, 60 children were enrolled and had blood samples taken before, immediately after, and 1 day after surgery. Conventional coagulation tests included: activated partial thromboplastin time, prothrombin time, fibrinogen, fibrin D-dimer, thrombin clotting time, factor XIII, and platelet count.ResultsPost-surgical haemostatic impairment was present to some degree in all children, as seen by pronounced changes in activated partial thromboplastin time, prothrombin time, thrombin clotting time, and platelet count, as well as RoTEM®analysis. RoTEM®showed marked changes in clotting time – prolonged by 7–18% – clot formation time – prolonged by 46–71% – maximum clot firmness – reduced by 10–19%, and maximum velocity – reduced by 29–39%. Comparison of the two techniques showed that conventional coagulation tests and RoTEM®performed equally well with regard to negative predictive values for excessive post-operative drain production – more than 20 millilitres per kilogram per 24 hours after surgery – with an area under the curve of approximately 0.65.ConclusionRoTEM®can detect haemostatic impairments in children undergoing cardiac surgery and the method should be considered as a supplement in the perioperative care of the children where targeted transfusion therapy is necessary to avoid volume overload.

scholarly journals Effect of storage time on prothrombin time and activated partial thromboplastin time: study at a tertiary care center in Kashmir valley

2018 ◽  
Vol 4 (7) ◽  
pp. 182
Author(s):  
Sajad Geelani ◽  
Gul Sajad Wani ◽  
Subuh Parvez Khan ◽  
Syed Mudasir Qadri ◽  
Javid Rasool ◽  
...  
Keyword(s):  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Storage Temperature ◽  
Care Center ◽  
Tertiary Care ◽  
Activated Partial Thromboplastin Time ◽  
Time Interval ◽  
Tertiary Care Center ◽  
Kashmir Valley ◽  
Coagulation Tests

<p class="abstract"><strong>Background:</strong> Prothrombin time (PT) and activated partial thromboplastin time (APTT) are tests of haemostasis commonly employed in the evaluation of coagulopathies. Storage temperature and time interval between sample collection and testing can have a significant effect on results of coagulation tests. The aims of the study were investigate whether storage temperature and time influence the results of routine coagulation tests and whether any changes caused by delayed analysis results in a clinically relevant difference, as well as to establish our own acceptable storage temperature and time guidelines.</p><p class="abstract"><strong>Methods:</strong> This study was conducted at Department of Clinical Haematology, in a tertiary care center in Kashmir valley. This study included 50 cases. Individuals with chronic liver diseases or cardiovascular disorders, subjects on anticoagulant therapy were excluded. 25 samples were observed at room temperature (RT) and 25 samples at 2-8°C. PT and APTT was measured at 0, 2, 4, 8, 16 and 24 hours both at RT and 2-8°C. Findings at 0 hr were compared to findings at 2,4, 8,16 and 24 hours in both the groups.  </p><p class="abstract"><strong>Results:</strong> In case of PT, reliable results were obtained up to 24 hrs either kept at RT or at 2 to 8°C and for APTT reliable results were obtained up to 4 hours kept at RT or at 2 to 8°C as there was no significant change during this period.</p><p class="abstract"><strong>Conclusions:</strong> Coagulation test should be performed as soon as possible with PT being performed before 24 hours and APTT before 4 hours of collection of sample irrespective of whether the sample has been preserved at RT or in refrigerator.</p>

scholarly journals APTT (Activated Partial Thromboplastin Time) dan (Prothrombin Time) pada Penderita Diabetes Melitus Tipe 2 di RSUD dr. Doris Sylvanus Palangkaraya

2020 ◽  
Vol 2 (2) ◽  
pp. 125-129
Author(s):  
Rinny Ardina ◽  
Fera Sartika ◽  
Lidya Prihatini Nainggolan
Keyword(s):  
Diabetes Mellitus ◽  
Cardiovascular Disease ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Activated Partial Thromboplastin Time ◽  
Coagulation System ◽  
Hemostasis System ◽  
Diabetes Mellitus Management ◽  
Routine Coagulation

Patients with type 2 diabetes mellitus (T2DM) have a high risk of atherothrombotic events. Hyperglycemia and other metabolic disorders in T2DM are associated with abnormalities of hemostasis system and thrombosis that contribute to cardiovascular disease. Routine laboratory tests to examine the hemostasis system are Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT). This study aimed to describe the abnormalities of APTT and PT in patients with T2DM in RSUD dr. Doris Sylvanus Palangkaraya. Twenty subjects with T2DM in RSUD dr. Doris Sylvanus Palangkaraya were obtained using purposive sampling techniques and blood samples were examined with a COATX Biosystem coagulation using photocolorimetric method. This study found that was 70% APTT are shortened, 5% APTT are prolonged and 25% APTT were normal. While the PT results were 25% PT are shortened and 75% APTT were normal. Abnormalities of APTT and PT in T2DM patients in RSUD dr. Doris Sylvanus Palangkaraya showed an abnormality of the coagulation system so as to allow a tendency for bleeding and cardiovascular disease in T2DM patients. Routine coagulation test should be continued to help better diabetes mellitus management in order to prevent micro or macrovascular complications.

Interferences in coagulation tests – evaluation of the 570-nm method on the Dade Behring BCS analyser

2005 ◽  
Vol 43 (2) ◽  
Author(s):  
Ralf Junker ◽  
Margit Käse ◽  
Helmut Schulte ◽  
Ruth Bäumer ◽  
Claus Langer ◽  
...  
Keyword(s):  
Optical Properties ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Activated Partial Thromboplastin Time ◽  
Plasma Samples ◽  
Comparison Methods ◽  
Coagulation Tests ◽  
Elisa Technique ◽  
Roche Diagnostics ◽  
D Dimer

AbstractThe Dade Behring BCS is a coagulation analyser with optical reaction detection (standard 405nm). The present study was conducted to evaluate measurement at 570nm for analyses in interfering plasma samples. Prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen and D-dimer in normal (n=50), lipaemic (n=60), icteric (n=113), and haemolytic (n=58) samples were measured at 405 and 570nm. As they are unaffected by the optical properties of the sample, the mechanical STAcompact analyser (Roche Diagnostics) and an ELISA technique were defined as the “comparison” methods. The percentage of valid PT results using the 570-nm method varied from 54% (lipaemic samples) to 97% (haemolytic samples). Valid aPTT measurements were found in 67% (lipaemic samples) up to 93% (icteric samples) of samples. Fibrinogen measurement revealed valid results in 58% (lipaemic samples) to 100% (haemolytic samples) of samples. The number of valid D-dimer results varied from 28% (lipaemic material) up to 100% (haemolytic material). Significant inter-method differences were found: aPTT in lipaemic (BCS 405 vs. 570nm) and icteric samples (STAcompact vs. BCS 405 and 570nm); fibrinogen in lipaemic (BCS 405 vs. 570nm), icteric (BCS 405 vs. 570nm; STAcompact vs. BCS 570nm) and haemolytic samples (STAcompact vs. BCS 405 and 570nm). Differences between the BCS 570-nm and the STAcompact methods were in most cases low and less pronounced than between the BCS 570- and 405-nm methods, making the BCS 570-nm method an alternative to measurement at 405nm. Limitations have to be taken into account regarding lipaemic plasma.

Interference of Blood Cell Lysis on Routine Coagulation Testing

2006 ◽  
Vol 130 (2) ◽  
pp. 181-184 ◽  
Author(s):  
Giuseppe Lippi ◽  
Martina Montagnana ◽  
Gian Luca Salvagno ◽  
Gian Cesare Guidi
Keyword(s):  
Blood Cell ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Cell Lysis ◽  
Activated Partial Thromboplastin Time ◽  
Analytical Quality ◽  
Coagulation Assays ◽  
Coagulation Testing ◽  
Quality Specifications ◽  
Routine Coagulation

Abstract Context.—Preanalytical factors influencing the reliability of laboratory testing are commonplace. It is traditionally accepted that hemolytic samples are unsuitable for coagulation assays because of the release of hemoglobin, intracellular components, and thromboplastic substances from damaged blood cells. Objective.—To evaluate the influence of blood cell lysis on routine coagulation testing. Design.—Twelve aliquots prepared by serial dilutions of homologous lysated samples collected from 10 different subjects, and displaying a final percentage of lysis ranging from 0% to 9.1%, were tested for prothrombin time, activated partial thromboplastin time, fibrinogen, and dimerized plasmin fragment D. Lysis was achieved by subjecting citrated whole blood to a freeze-thaw cycle. Outcome Measures.—Interference from blood cell lysis on routine coagulation testing. Results.—Statistically significant increases in prothrombin time and dimerized plasmin fragment D were observed in samples containing final lysate concentrations of 0.5% and 2.7% respectively, whereas significant decreases were observed in activated partial thromboplastin time and fibrinogen in samples containing a final lysate concentration of 0.9%. The current analytical quality specifications for desirable bias are ±2.0% for prothrombin time, ±2.3% for activated partial thromboplastin time, and ±4.8% for fibrinogen. Percent variations from the baseline values exceeding the current analytical quality specifications for desirable bias were achieved for lysate concentrations of 0.9% (prothrombin time and activated partial thromboplastin time) and 1.8% (fibrinogen), corresponding to average free plasma hemoglobin concentrations of 1.7 and 3.4 g/L, respectively. Conclusion.—Our results confirm that, although slightly hemolyzed specimens might still be analyzable, a moderate blood cell lysis, as low as 0.9%, influences the reliability of routine coagulation testing. Because the interference in coagulation assays has a wide interindividual bias, we do not recommend lysis correction and we suggest that the most appropriate corrective measure should be free hemoglobin quantification and sample recollection.

Effect of Centrifuge Temperature on Routine Coagulation Tests

2018 ◽  
Vol 139 (3) ◽  
pp. 158-163 ◽  
Author(s):  
Hayrullah Yazar ◽  
Fatma Özdemir ◽  
Elif Köse
Keyword(s):  
Partial Thromboplastin Time ◽  
International Normalized Ratio ◽  
Activated Partial Thromboplastin Time ◽  
Time Intervals ◽  
Blood Samples ◽  
Test Parameters ◽  
Coagulation Tests ◽  
Volume Blood ◽  
D Dimer ◽  
Routine Coagulation

Background: This study investigated the effects of cooled and standard centrifuges on the results of coagulation tests to examine the effects of centrifugation temperature. Methods: Equal-volume blood samples from each patient were collected at the same time intervals and subjected to standard (25°C) and cooled centrifugation (2–4°C). Subsequently, the prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), fibrinogen, and D-dimer values were determined in runs with the same lot numbers in the same coagulation device using the Dia-PT R (PT and INR), Dia-PTT-liquid (aPTT), Dia-FIB (fibrinogen), and Dia-D-dimer kits, respectively. Results: The study enrolled 771 participants. The PT was significantly (p < 0.018) higher in participants on anticoagulant therapy. The respective median values of the test parameters determined using the standard and cooled centrifuges were as follows: PT 10.30 versus 10.50 s; PT (INR) 1.04 versus 1.09 s; APTT 28.90 versus 29.40 s; fibrinogen 321.5 versus 322.1 mg/dL; and D-dimer 179.5 versus 168.7 µg FEU/mL. There were significant differences (p < 0.001) in the parameters between the values obtained with the standard and cooled centrifuges. Conclusions: Centrifuge temperature can have a significant effect on the results of coagulation tests. However, broad and specific disease-based studies are needed.

scholarly journals Use of an Automated Coagulation Analyzer to Perform Heparin Neutralization With Polybrene in Blood Samples for Routine Coagulation Testing: Practical, Rapid, and Inexpensive

2013 ◽  
Vol 137 (11) ◽  
pp. 1641-1647 ◽  
Author(s):  
Panutsaya Tientadakul ◽  
Chulalak Kongkan ◽  
Wimol Chinswangwatanakul
Keyword(s):  
Prothrombin Time ◽  
Blood Samples ◽  
Coagulation Testing ◽  
Coagulation Tests ◽  
Clinically Significant ◽  
Aptt Reagent ◽  
Heparin Neutralization ◽  
Coagulation Analyzer ◽  
Suitable Sequence ◽  
Routine Coagulation

Context.—Heparin contamination in blood samples may cause false prolongation of activated partial thromboplastin time (aPTT) and prothrombin time results. Polybrene can neutralize heparin, but it affects coagulation by itself. Objectives.——To determine the optimal concentration of polybrene to neutralize heparin, to determine the suitable sequence of reagents for the neutralization method performed on the analyzer at the same time as prothrombin time and aPTT testing, and to detect the heparin contamination in blood samples for coagulation tests in our hospital using this method. Design.—Various concentrations of heparin were added to 10 normal and 76 abnormal plasma samples to study the efficacy of polybrene. Two programs of reagent sequencing for aPTT with polybrene performed on the analyzer were tested. Samples suspected of heparin contamination according to our criteria were selected for neutralization during a 3-month period. Results.——The optimal final concentration of polybrene was 25 μg/mL. Polybrene should be added after the aPTT reagent to minimize its interference effect. Even though results of prothrombin time and aPTT after neutralization did not equal those before the spike of heparin, the differences might not be clinically significant. Eighty-one of 4921 samples (1.6%) were selected for aPTT with the neutralization method, and the detection rate of heparin contamination was 84% (68 of 81), giving an overall incidence of 1.4% (68 of 4921). Conclusions.—This method is inexpensive and can be performed rapidly with prothrombin time and aPTT on the automated analyzer, which makes it easy to practice with no need for extra plasma volumes.

scholarly journals Rotational Thromboelastometry (ROTEM) for Assessing the Anticoagulant Effect of Rivaroxaban: A Single-Centre Cohort Study

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 5018-5018
Author(s):  
Thomas Andrew Fox ◽  
Andrew Wood ◽  
Anne Riddell ◽  
Pratima Chowdary ◽  
Anja B Drebes
Keyword(s):  
Creatinine Clearance ◽  
Prothrombin Time ◽  
Board Of Directors ◽  
Partial Thromboplastin Time ◽  
Research Funding ◽  
Activated Partial Thromboplastin Time ◽  
Anticoagulant Effect ◽  
Advisory Committees ◽  
Rotational Thromboelastometry ◽  
Coagulation Tests

Abstract Background: The direct, oral, factor Xa inhibitor, rivaroxaban, is increasingly used to provide effective anticoagulation in atrial fibrillation and venous thromboembolism. Whilst rivaroxaban does not require therapeutic monitoring there are situations when it is useful to estimate the anticoagulant effect of the drug such as during bleeding episodes or before emergency surgery. It has previously been shown that conventional coagulation tests can provide a crude estimation of anticoagulant effect of rivaroxaban if a sensitive reagent is used. In this study we explore whether rotational thromboelastometry (ROTEM) would provide a more accurate measure of rivaroxaban effect. Methods: Peak serum rivaroxaban levels were taken 3-5 hours post-dose in 121 consecutive patients established on a once-daily anticoagulation regime with rivaroxaban. Conventional coagulation tests, prothrombin time (PT) and activated partial thromboplastin time (APTT) were performed alongside rivaroxaban level and rotational thromboelastometry (ROTEM, TEM Ltd, Germany) on native citrated whole blood. PT and APTT used HemosIL Recombiplastin 2G and SynthasIL reagents (Instrumentation Laboratory (IL), USA) respectively. Rivaroxaban levels were measured using HemosIL Liquid Anti Xa kit (IL, USA) with rivaroxaban calibrators from Hyphen Biomed, France on an ACL TOP 700 coagulometer (IL, USA)). Demographic and biochemical data was collected on each patient. Results were analysed to determine if ROTEM can be used to assess the anticoagulant effect of rivaroxaban in real-world patients with different demographics and organ function. Results: Significant positive correlation was seen between rivaroxaban level and prothrombin time (PT) (R=0.796, Pearson's correlation coefficient). Weaker correlation was observed between rivaroxaban level and activated partial thromboplastin time (APTT) (R=0.425). There was modest positive correlation between the clotting time (CT) parameter using ROTEM and rivaroxaban level (R=0.328). However, when grouped into low (<200ng/ml), intermediate (200-300ng/ml) and high (>300ng/ml) rivaroxaban levels, the CTs show no meaningful association and therefore cannot be used as a surrogate marker to predict anticoagulant effect. There is no significant difference between the mean rivaroxaban levels for patients on 15mg rivaroxaban, those on 20mg with creatinine clearance <60ml/min and those on 20mg with creatinine clearance >60ml/min (Analysis of Variance, n=121, F=2.009, P=0.159), suggesting that with dose adjustment a similar anticoagulant effect is achieved in patients with different renal function. Conclusion: Our data suggests that the correlation between rivaroxaban levels and ROTEM CT parameter is not sufficiently strong to reliably predict the anticoagulant effect of rivaroxaban and does not confer any advantage over conventional clotting tests. Disclosures Chowdary: Baxalta: Honoraria, Membership on an entity's Board of Directors or advisory committees; Biogen: Honoraria, Membership on an entity's Board of Directors or advisory committees; CSL Behring: Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding; Sobi: Honoraria; Bayer: Honoraria; Pfizer: Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding; Novo Nordisk: Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding. Drebes:Bayer: Consultancy; Bayer: Other: Educational Grant.

Inhibition of Coagulation and Fibrinolysis by Aromatic Amidino Compounds

1971 ◽  
Vol 25 (03) ◽  
pp. 391-404 ◽  
Author(s):  
J.D Geratz
Keyword(s):  
Prothrombin Time ◽  
Human Plasma ◽  
Partial Thromboplastin Time ◽  
Inhibitory Effect ◽  
Clot Lysis ◽  
Similar Degree ◽  
Contact Activation ◽  
Plasma Clot ◽  
Human Thrombin ◽  
Canine Plasma

Summary1. Aromatic diamidines which are potent inhibitors of trypsin possess a marked inhibitory effect on the clotting activity of human thrombin and on the prothrombin time and partial thromboplastin time of human plasma. They also block the contact activation phase of the coagulation process. The strongest inhibitor among the compounds tested was M & B 4596 which was followed in second place by pentamidine.2. Pentamidine was 10 times more active than ε-ACA in impeding streptokinase-induced lysis of human plasma clots. It was 100-200 times stronger than ε-ACA in inhibiting the activation of bovine plasminogen by activators formed from the interaction between streptokinase and either human plasmin(ogen) or human plasma.3. The prothrombin time and partial thromboplastin time of canine plasma were less susceptible to inhibition by pentamidine than the same tests on human plasma. Clot lysis in the canine system was inhibited by pentamidine to a similar degree as in the human system. After intravenous injection of pentamidine in the dog there occurred the expected prolongation of the partial thromboplastin time and of the clot lysis time.

EFFECTS OF NICKEL CHLORIDE ON INDICES OF HEMOCOAGULATION AND LIPID PEROXIDATION IN RATS

2019 ◽  
pp. 83-90
Author(s):  
Э.М. Гаглоева ◽  
В.Б. Брин ◽  
С.В. Скупневский ◽  
Н.В. Боциева ◽  
Т.В. Молдован
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Platelet Aggregation ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Nickel Chloride ◽  
Activated Partial Thromboplastin Time ◽  
Fibrinogen Concentration ◽  
Thrombin Time ◽  
Hemostasis System

Цель исследования - изучить состояние системы гемостаза при хронической интоксикации хлоридом никеля, исследовать взаимосвязь показателей гемокоагуляции с процессами липопероксидации у крыс в эксперименте. Методика. Опыты проводили на крысах-самцах Вистар (n=50, 230-250 г). Раствор NiCl2 (5 мг/кг) вводили внутрижелудочно ежедневно в течение 2 нед, 1 и 2 мес. По завершении эксперимента исследовали состояние тромбоцитарного и коагуляционного звеньев гемостаза, антикоагулянтную и фибринолитическую активность крови, а также определяли активность процессов перекисного окисления липидов и антиоксидантных ферментов. Результаты. Установлено, что через 2 нед и 1 мес интоксикации у крыс отмечались гиперкоагуляционные изменения показателей свертывающей системы крови: повышение агрегационной активности тромбоцитов, увеличение концентрации фибриногена, снижение активированного частичного тромбопластинового времени (АЧТВ) и протромбинового времени. В этот период регистрировалось увеличение антитромбиновой и фибринолитической активности крови. Через 2 мес наблюдалось подавление активности клеточного звена гемостаза - тромбоцитопения, ослабление степени АДФ-индуцируемой агрегации тромбоцитов. Выявлялась тенденция к уменьшению концентрации фибриногена. На фоне снижения АЧТВ и тромбинового времени отмечалось увеличение протромбинового времени. В то же время регистрировалось угнетение противосвертывающего звена системы гемостаза (снижалась активность антитромбина III), наблюдалось истощение резервных возможностей фибринолитического звена (замедление фXIIа-зависимого эуглобулинового лизиса) и увеличение содержания растворимых фибрин мономерных комплексов, что свидетельствует о наличии тромбинемии. Через 2 нед, один и два месяца интоксикации у животных выявлялись корреляционные связи между основными показателями системы гемостаза и активностью процессов перекисного окисления липидов и антиоксидантных ферментов. Заключение. Полученные данные подтверждают наличие взаимосвязи активности процессов липопероксидации и системы гемостаза, в том числе при хронической никелевой интоксикации. Результаты исследования позволяют рекомендовать применение антиоксидантов для разработки способов коррекции гемостатических сдвигов при воздействии на организм тяжелых металлов. The aim. To study the state of the hemostasis system in chronic nickel intoxication and to investigate the relationship between hemocoagulation indices and lipoperoxidation processes in rats. Methods. Experiments were carried out on male Wistar rats (n=50, 230-250 g). A solution of nickel chloride (5 mg/kg) was administered daily intragastrically for two weeks, one and two months. At the end of the experiments, indices of platelet and coagulation hemostasis systems, anticoagulant and fibrinolytic activity of blood plasma, and activities of lipid peroxidation and antioxidant enzymes were studied. Results. Hypercoagulative changes in indices of the coagulation system were observed in rats after two weeks and one month of intoxication, including increased platelet aggregation and fibrinogen concentration and shortened activated partial thromboplastin time and prothrombin time. During the same period, increased antithrombin and fibrinolytic activities were observed. The depressed activity of the cellular component of hemostasis evident as thrombocytopenia and impaired ADP-induced platelet aggregation was detected after two months of intoxication. A tendency to decrease in fibrinogen concentration was observed. The shortened activated partial thromboplastin time and thrombin time were associated with prolonged prothrombin time. At the same time, inhibition of the anticoagulant component of hemostasis (decreased antithrombin III activity), exhaustion of the fibrinolysis system reserve (delayed fXIIa-dependent euglobulin lysis), and a significant increase in soluble fibrin monomeric complexes indicative of thrombinemia were observed. After two weeks, one and two months of nickel intoxication, a correlation was found between the major indices of the hemostasis system and the activities of lipid peroxidation and antioxidant enzymes. Conclusion. The study confirmed a relationship between the lipid peroxidation activity and the hemostasis system, specifically in chronic nickel intoxication. This result allows to recommend the use of antioxidants in developing methods for correction of hemostatic induced affected by heavy metals.

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