The Detection of Hemoglobin Variants by Isoelectrofocusing Using EDTA-Collected and Filter Paper-Dried Cord Blood Specimens

1990 ◽  
Vol 94 (2) ◽  
pp. 199-202 ◽  
Author(s):  
Abdullah Kutlar ◽  
Ozan Ozcan ◽  
Jennie T. Brisco ◽  
Marcia C. Ansley ◽  
Titus H. J. Huisman
Keyword(s):  
Cord Blood ◽  
Filter Paper ◽  
Hemoglobin Variants ◽  
Blood Specimens

beta-Lipoprotein quantitation in cord blood spotted on filter paper: a screening test.

1980 ◽  
Vol 26 (9) ◽  
pp. 1285-1290 ◽  
Author(s):  
G D Vladutiu ◽  
C J Glueck ◽  
M T Schultz ◽  
S McNeely ◽  
R Guthrie
Keyword(s):  
Cord Blood ◽  
Total Cholesterol ◽  
Mass Screening ◽  
Filter Paper ◽  
Screening Test ◽  
Type Ii ◽  
Blood Samples ◽  
Paired Samples ◽  
Familial Type ◽  
Blood Specimens

Abstract We describe an electroimmunodiffusion technique for measuring beta-lipoprotein in cord blood spotted on filter paper. A series of cord-blood samples, taken from 916 consecutive live-birth infants, was spotted directly onto filter paper and assayed for beta-lipoprotein. Eleven had above-normal beta-lipoprotein. Of these 11, seven were tested two to six months later, along with their parents, for total cholesterol and triglyceride concentrations. Five infants had increased cholesterol values, and four parents of these infants had either increased cholesterol or triglyceride values. We also measured beta-lipoprotein concentrations in 63 paired samples of dried cord-blood and three-day post-delivery blood specimens, routinely collected for phenylketonuria screening. We saw a significant correlation between results for the specimens, but detected no cases of increased beta-lipoprotein. beta-Lipoprotein assay in dried specimens of cord blood is simple, inexpensive, and potentially is useful in mass screening of newborns for familial type II and combined hyperlipidemia.

SERUM C-REACTIVE PROTEIN IN INFECTIONS DURING THE FIRST SIX MONTHS OF LIFE

PEDIATRICS ◽  
1966 ◽  
Vol 37 (2) ◽  
pp. 270-277
Author(s):  
Natalie S. Felix ◽  
Hironori Nakajima ◽  
B. M. Kagan
Keyword(s):  
Cord Blood ◽  
Positive Reaction ◽  
Clinical Judgment ◽  
C Reactive Protein ◽  
Protein Determination ◽  
Suspected Infection ◽  
Reactive Protein ◽  
Blood Specimens ◽  
Usual Laboratory ◽  
Single Laboratory

Serum C-reactive protein determinations were done on 66 cord blood specimens and on capillary blood specimens of 669 apparently normal infants and 266 infants ill with known or suspected infection during the first 6 months of life. All but one of the cord blood specimens were negative for C-reactive protein. The incidence of positive reactors increased soon after birth with 50% of the normal newborns having positive reactions during the first week of life. This rapidly decreased after this period so that by 1 month to 6 months of age the incidence was only 2%. The intensity of the positive reaction in the majority was only up to 1 mm. Two-thirds of the infants with infection had positive reactions. The more severe the infection the greater the intensity of the positive reaction. C-reactive protein was also present in a variety of conditions besides infections. Serum C-reactive protein determination can be a valuable diagnostic aid in infections during the first 6 months of life when the usual laboratory criteria for infections are often not helpful. However, it has its limitations. Like any other single laboratory test, it must be used in conjunction with critical clinical judgment.

Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper.

1980 ◽  
Vol 26 (8) ◽  
pp. 1198-1200 ◽  
Author(s):  
A P Orfanos ◽  
E W Naylor ◽  
R Guthrie
Keyword(s):  
Filter Paper ◽  
Colorimetric Method ◽  
Dried Blood Spots ◽  
Arginase Activity ◽  
Kinetic Reaction ◽  
Hematological Disorders ◽  
Blood Spots ◽  
Blood Specimens ◽  
The Stability

Abstract We describe a microfluorometric method for determination of arginase activity in dried blood spots on filter paper. The arginase in discs punched from such dried blood specimens is activated by preincubation with Mn2+ at 37 degrees C. After incubation with substrate at the same temperature, urea is determined fluorometrically by oxidation of NADH to NAD+ in a coupled kinetic reaction. We compare the results of this method with those of a colorimetric method involving liquid blood samples, and assess the stability of the enzyme in dried blood on filter paper. The presence of serum has no effect on the activity. This method may be useful in the early detection of arginase deficiency and certain hematological disorders.

Physiologic differences in hemoglobin variants

1965 ◽  
Vol 208 (1) ◽  
pp. 198-202 ◽  
Author(s):  
Robert B. Thompson ◽  
Richard L. Warrington ◽  
Warren N. Bell
Keyword(s):  
Cord Blood ◽  
Whole Blood ◽  
Fold Increase ◽  
Twofold Increase ◽  
Hemoglobin A ◽  
Hb E ◽  
Hb S ◽  
Hemoglobin Variants

Studies were carried out on whole blood, concentrated hemoglobin solutions, and isolated hemoglobin fractions as related to oxygen association. In hemoglobins Ao, A1, Dalpha, GPhiladelphia, and C, no difference was found in the oxygen association curve. Hemoglobins A1, B2, and Lepore showed a 2½ fold increased oxygen association over normal Hb-A. Hemoglobin solutions from cord blood showed no difference from Hb-A, whereas cord blood showed a twofold increase. Hb-H and Hb-Barts showed identical oxygen association curves with a 10- to 12-fold increase over Hb-A; no heme-heme interaction and no Bohr was found with Hb-H or Barts. Hb-E and Hb-S showed a slight decrease in oxygen equilibria over hemoglobin A.

Immunofluorometry of thyrotropin, from whole-blood spots on filter paper, to screen for congenital hypothyroidism.

1986 ◽  
Vol 32 (10) ◽  
pp. 1854-1856 ◽  
Author(s):  
J Arends ◽  
B Nørgaard-Pedersen
Keyword(s):  
Monoclonal Antibodies ◽  
Congenital Hypothyroidism ◽  
Filter Paper ◽  
Clinical Evidence ◽  
Type System ◽  
Time Resolved ◽  
Sandwich Type ◽  
Blood Specimens ◽  
Positive Results ◽  
Paper Disc

Abstract We have evaluated a time-resolved immunofluorometric assay (IFMA) for determining thyrotropin. This "sandwich"-type system involves two monoclonal antibodies directed against different epitopes. A linear relationship between signal and thyrotropin concentration was observed up to 6000 milli-int. units/L. This procedure takes one day, vs six days with our present RIA technique, and requires only a tenth as much sample. Furthermore, intra- and interassay CVs are lower than with RIA. Assay of 19 paper-disc blood specimens from newborns identified as having congenital hypothyroidism, both by RIA and by clinical evidence, also gave positive results with IFMA. In prospective assay of 3944 specimens by both methods we identified one case of congenital hypothyroidism, which was detected by both techniques. Technical false-positive reactions, identified as such by repeated analyses, were fewer with the IFMA method than with RIA.

Measles seroconfirmation using dried capillary blood specimens in filter paper

1984 ◽  
Vol 3 (2) ◽  
pp. 117-121 ◽  
Author(s):  
STEVEN G. F. WASSILAK ◽  
ROGER H. BERNIER ◽  
KENNETH L. HERRMANN ◽  
WALTER A. ORENSTEIN ◽  
KENNETH J. BART ◽  
...  
Keyword(s):  
Filter Paper ◽  
Capillary Blood ◽  
Blood Specimens

Effect of lot-to-lot variability in filter paper on the quantification of thyroxin, thyrotropin, and phenylalanine in dried-blood specimens.

1988 ◽  
Vol 34 (1) ◽  
pp. 53-58 ◽  
Author(s):  
W E Slazyk ◽  
D L Phillips ◽  
B L Therrell ◽  
W H Hannon
Keyword(s):  
Filter Paper ◽  
Whole Blood ◽  
Neonatal Screening ◽  
Intact Cell ◽  
High Concentration ◽  
Mean Values ◽  
Screening Programs ◽  
Blood Specimens

Abstract We prepared whole-blood pools to contain various concentrations of phenylalanine (Phe), thyroxin (T4), and thyrotropin (TSH) and applied them to six different lots of Schleicher & Schuell Grade 903 filter paper, two of which represented extremes for serum-absorbancy. Individual measured T4 values showed minimal overlap among all pools for each individual filter-paper lot and for all lots combined, but Phe values overlapped considerably among the high-concentration pools within and among lots. Individual TSH values also showed considerable overlap among the high-concentration pools for all lots combined, but little overlap within each lot. Maximum differences in mean observed values among lots ranged from 6% to 36% for all analytes. Assay results from hemolyzed blood specimens generally were lower than from intact-cell blood specimens for T4 and TSH, but slightly higher for Phe. Maximum among-lot differences in mean values ranged from 13% to 29% for all analytes when each tested lot was used for assay calibration. Lot-to-lot differences in measured values were not strongly related to serum absorbancy values. We conclude that routinely encountered within- and among-lot filter paper variability, as measured by serum-absorbancy, is not alone sufficient to cause gross quantification errors in neonatal screening programs.

scholarly journals Influence of Hepatitis B immunization to prevent vertical transmission of Hep-B virus in infants bam from Hep-B positive mother

2016 ◽  
Vol 50 (6) ◽  
pp. 321 ◽  
Author(s):  
Liza Fitria ◽  
Hartono Gunardi ◽  
Arwin A. P. Akib
Keyword(s):  
Hepatitis B ◽  
Cord Blood ◽  
Vertical Transmission ◽  
Perinatal Transmission ◽  
Term Infants ◽  
Positive Mother ◽  
Positive Hbsag ◽  
Blood Specimens ◽  
Hbsag Positive Mother ◽  
Hep B

Background Hepatitis B is one of the most common infectious diseases worldv.ide. Indonesia has moderate-high endemicity for hepatitis B infection. Perinatal transmission increases the risk for chronic hepatitis B. Infants from HBsAg-positive mothers should receive hepatitis B immunoglobulin (HBIG) and vaccination within 12 hours of birth, but this practice is not routinely done in Indonesia due to financial constraints.Objectives To detennine the influence of Hep-B immunization on preventing Hepatitis B vertical transmission. Methods A descriptive cohort study was conducted from May 2009 - January 2010. Subjects were term infants born from HBsAg-positive mothers v.ith no history of HBIG administration. They had received complete hepatitis B immunization and 1 month after the last dose were evaluated for HBsAg and anti-HBs. Cord blood was also taken during labor to measure HBsAg.Results There were 22 infants born from HBsAg-positive mother who met the inclusion criteria. HBsAgwas positive in 6 of22 cord blood specimens. There were 15 infants who completed this study. One of 15 infants had positive HBsAg after completed hepatitis B immunization and 12 of 15 infants had protective level of anti-HBs. Effectiveness of hepatitis B immunization to prevent vertical transmission in this study was 70-90%.Conclusion Hepatitis B immunization can prevent vertical transmission of hepatitis B in infants born to mothers who are HBsAg-positive even without administration of HBIG.

Use of blood specimens collected on filter paper in screening for abnormal hemoglobins.

1976 ◽  
Vol 22 (5) ◽  
pp. 685-687 ◽  
Author(s):  
R M Schmidt ◽  
E M Brosious ◽  
S Holland ◽  
J M Wright ◽  
G R Serjeant
Keyword(s):  
Disease Control ◽  
Cellulose Acetate ◽  
Filter Paper ◽  
Whole Blood ◽  
Sample Collection ◽  
Capillary Tubes ◽  
Cellulose Acetate Electrophoresis ◽  
Blood Samples ◽  
Blood Specimens ◽  
The U.S

Abstract Both cellulose acetate electrophoresis and citrate agar electrophoresis were performed on 834 blood samples collected on filter paper in Jamaica and shipped for testing to the National Hemoglobinopathy Standardization Laboratory at the U.S. National Center for Disease Control. Additionally, 30 blood samples collected locally were stored on filter paper, in microhematocrit capillary tubes, and as whole blood specimens; at selected times the samples were tested for stability to determine the best sample-collection technique for hemoglobin electrophoresis. Results were most nearly accurate when both cellulose acetate electrophoresis and citrate agar testing were used. The methods are easy to perform, but results are unreliable if the blood samples on filter paper are stored at 4 degrees C for longer than two weeks before they are tested.

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